Control of genetic identity by protein electrophoresis in varietal reproduction of wheat
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Održanje i kontrola genetske čistoće, odnosno genetskog identiteta, jedan je
od krajnjih ciljeva sjemenarstva nakon priznavanja neke sorte pšenice. Primjena
pozitivne i negativne selekcije, na razini fenotipa pri sortnoj reprodukciji
pšenice, omogućila je sortnu čistoću od 99 i 100% u usjevima svih sjemenskih
kategorija.
U cilju ispitivanja genetske identičnosti, u ovom su radu analizirane
rezervne bjelačevine pšenice – glijadini, koji su metodom poliakrilne gel
elektroforeze razdvojeni na veliki broj frakcija koje pokazuju visoku
heterogenost. Od analiziranih sorti pšenice jedino je sorta PKB Rodika imala
genetsku čistoću od 100% za sve kategorije sjemena. Sorta PKB Kristina je
imala genetsku čistoću od 99% za kategoriju predosnovno sjeme, a sorta BG
Merkur 99% genetske čistoće za kategoriju – sjeme prve generacije C1.
Elektroforeza bjelančevina pokazala je da postoji divergentnost između
ispitivanih sorti pšenice po sastavu komponenti glijadinske frakcije, dok unutar
so...rti postoji ujednačenost.
Maintenance and control of genetic purity and genetic identity is one of the
ultimate goals in seed production after the recognition of some varieties of
wheat. The application of positive and negative selection at the phenotypic level
in varietal reproduction of wheat, enabled 99 and 100% of varietal purity in
crops of all seed categories. In order to study the genetic identity, in this paper the reserve proteins of
wheat – gliadins were analyzed. They were separated by polyacrylic gel
electrophoresis into numerous components that showed great heterogeneity.
Of all the analyzed wheat varieties, only PKB Rodika variety had the
genetic purity of 100% in all categories of seed. PKB Kristina had the genetic
purity of 99% in the pre-basic seed category and the variety BG Merkur had
genetic purity of 99% in the seed of first generation – C1.
Protein electrophoresis showed there was the divergence among the
varieties of wheat in its composition of gliadin fractions, while there w...as
uniformity among defferent varietal reproductions within each variety.
Кључне речи:
pšenica / genetski identitet / elektroforeza proteina / wheat / genetic identity / protein electrophoresisИзвор:
Agronomski glasnik, 2015, 77, 4-6, 173-183Издавач:
- Zagreb : Hrvatsko agronomsko društvo
Институција/група
Institut za povrtarstvoTY - JOUR AU - Đurić, Nenad AU - Cvijanović, Gorica AU - Matković, Mirela AU - Dozet, Gordana AU - Branković, Gordana PY - 2015 UR - http://RIVeC.institut-palanka.rs/handle/123456789/324 AB - Održanje i kontrola genetske čistoće, odnosno genetskog identiteta, jedan je od krajnjih ciljeva sjemenarstva nakon priznavanja neke sorte pšenice. Primjena pozitivne i negativne selekcije, na razini fenotipa pri sortnoj reprodukciji pšenice, omogućila je sortnu čistoću od 99 i 100% u usjevima svih sjemenskih kategorija. U cilju ispitivanja genetske identičnosti, u ovom su radu analizirane rezervne bjelačevine pšenice – glijadini, koji su metodom poliakrilne gel elektroforeze razdvojeni na veliki broj frakcija koje pokazuju visoku heterogenost. Od analiziranih sorti pšenice jedino je sorta PKB Rodika imala genetsku čistoću od 100% za sve kategorije sjemena. Sorta PKB Kristina je imala genetsku čistoću od 99% za kategoriju predosnovno sjeme, a sorta BG Merkur 99% genetske čistoće za kategoriju – sjeme prve generacije C1. Elektroforeza bjelančevina pokazala je da postoji divergentnost između ispitivanih sorti pšenice po sastavu komponenti glijadinske frakcije, dok unutar sorti postoji ujednačenost. AB - Maintenance and control of genetic purity and genetic identity is one of the ultimate goals in seed production after the recognition of some varieties of wheat. The application of positive and negative selection at the phenotypic level in varietal reproduction of wheat, enabled 99 and 100% of varietal purity in crops of all seed categories. In order to study the genetic identity, in this paper the reserve proteins of wheat – gliadins were analyzed. They were separated by polyacrylic gel electrophoresis into numerous components that showed great heterogeneity. Of all the analyzed wheat varieties, only PKB Rodika variety had the genetic purity of 100% in all categories of seed. PKB Kristina had the genetic purity of 99% in the pre-basic seed category and the variety BG Merkur had genetic purity of 99% in the seed of first generation – C1. Protein electrophoresis showed there was the divergence among the varieties of wheat in its composition of gliadin fractions, while there was uniformity among defferent varietal reproductions within each variety. PB - Zagreb : Hrvatsko agronomsko društvo T2 - Agronomski glasnik T1 - Control of genetic identity by protein electrophoresis in varietal reproduction of wheat EP - 183 IS - 4-6 SP - 173 VL - 77 UR - https://hdl.handle.net/21.15107/rcub_rivec_324 ER -
@article{ author = "Đurić, Nenad and Cvijanović, Gorica and Matković, Mirela and Dozet, Gordana and Branković, Gordana", year = "2015", abstract = "Održanje i kontrola genetske čistoće, odnosno genetskog identiteta, jedan je od krajnjih ciljeva sjemenarstva nakon priznavanja neke sorte pšenice. Primjena pozitivne i negativne selekcije, na razini fenotipa pri sortnoj reprodukciji pšenice, omogućila je sortnu čistoću od 99 i 100% u usjevima svih sjemenskih kategorija. U cilju ispitivanja genetske identičnosti, u ovom su radu analizirane rezervne bjelačevine pšenice – glijadini, koji su metodom poliakrilne gel elektroforeze razdvojeni na veliki broj frakcija koje pokazuju visoku heterogenost. Od analiziranih sorti pšenice jedino je sorta PKB Rodika imala genetsku čistoću od 100% za sve kategorije sjemena. Sorta PKB Kristina je imala genetsku čistoću od 99% za kategoriju predosnovno sjeme, a sorta BG Merkur 99% genetske čistoće za kategoriju – sjeme prve generacije C1. Elektroforeza bjelančevina pokazala je da postoji divergentnost između ispitivanih sorti pšenice po sastavu komponenti glijadinske frakcije, dok unutar sorti postoji ujednačenost., Maintenance and control of genetic purity and genetic identity is one of the ultimate goals in seed production after the recognition of some varieties of wheat. The application of positive and negative selection at the phenotypic level in varietal reproduction of wheat, enabled 99 and 100% of varietal purity in crops of all seed categories. In order to study the genetic identity, in this paper the reserve proteins of wheat – gliadins were analyzed. They were separated by polyacrylic gel electrophoresis into numerous components that showed great heterogeneity. Of all the analyzed wheat varieties, only PKB Rodika variety had the genetic purity of 100% in all categories of seed. PKB Kristina had the genetic purity of 99% in the pre-basic seed category and the variety BG Merkur had genetic purity of 99% in the seed of first generation – C1. Protein electrophoresis showed there was the divergence among the varieties of wheat in its composition of gliadin fractions, while there was uniformity among defferent varietal reproductions within each variety.", publisher = "Zagreb : Hrvatsko agronomsko društvo", journal = "Agronomski glasnik", title = "Control of genetic identity by protein electrophoresis in varietal reproduction of wheat", pages = "183-173", number = "4-6", volume = "77", url = "https://hdl.handle.net/21.15107/rcub_rivec_324" }
Đurić, N., Cvijanović, G., Matković, M., Dozet, G.,& Branković, G.. (2015). Control of genetic identity by protein electrophoresis in varietal reproduction of wheat. in Agronomski glasnik Zagreb : Hrvatsko agronomsko društvo., 77(4-6), 173-183. https://hdl.handle.net/21.15107/rcub_rivec_324
Đurić N, Cvijanović G, Matković M, Dozet G, Branković G. Control of genetic identity by protein electrophoresis in varietal reproduction of wheat. in Agronomski glasnik. 2015;77(4-6):173-183. https://hdl.handle.net/21.15107/rcub_rivec_324 .
Đurić, Nenad, Cvijanović, Gorica, Matković, Mirela, Dozet, Gordana, Branković, Gordana, "Control of genetic identity by protein electrophoresis in varietal reproduction of wheat" in Agronomski glasnik, 77, no. 4-6 (2015):173-183, https://hdl.handle.net/21.15107/rcub_rivec_324 .