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Mogućnost primene molekularnih RAPD markera u selekciji crnog luka

dc.creatorPavlović, Nenad
dc.creatorZdravković, Jasmina
dc.creatorCvikić, Dejan
dc.creatorZdravković, Milan
dc.creatorAdžić, Slađan
dc.creatorPavlović, Suzana
dc.creatorŠurlan-Momirović, Gordana
dc.date.accessioned2021-06-07T12:21:26Z
dc.date.available2021-06-07T12:21:26Z
dc.date.issued2012
dc.identifier.issn0534-0012
dc.identifier.urihttp://RIVeC.institut-palanka.rs/handle/123456789/120
dc.description.abstractIn order to estimate, at the molecular level, the divergence of parental lines that were used in diallel crossbreeding for production of superior offspring (F1 generation hybrids) at the Institute for Vegetable Crops, the molecular analysis using five RAPD markers for five pairs of parents has been performed. It gives an insight into their genetic polymorphism and the possibility of their further use in breeding programs. Information from this research has pioneered the application of molecular markers of onion in Serbia. Analyses were performed using the RAPD primers, which in previous studies established a high degree of polymorphism. In all five cases there was a corresponding amplification of DNA segments. From totally 50 bands analyzed, the length of fragments ranged from 500 to 3000 bp. Number of polymorphic band per example was 8 to 13. In our research at the level of the analyzed primers, a high degree of polymorphism between analyzed genotypes has been found. Based on UPGMA dendogram, analyzed genotypes were divided into two main clusters and two subclusters.en
dc.description.abstractU cilju procene divergencije na molekularnom nivou roditeljskih linija koje su korišćene u dialenom ukrštanju sa ciljem dobijanja superiornog potomstva (hibrida F1 generacije) u Institutu za povrtarstvo, urađena je molekularna analiza primenom pet RAPD markera kod pet roditeljskih parova. Ovim putem je sagledan njihov genetički polimorfizam kao i mogućnost njihove dalje primene u oplemenjivačkim programima. Informacije dobijene ovim istraživanjem predstavljaju pionirski poduhvat u primene molekularnih markera na crnom luku u Srbiji. Analize su izvršene upotrebom RAPD prajmera, za koje je u ranijim istraživanjima utvrđen visok stepen polimorfizma. U svih pet slučajeva je došlo do amplifikacije odgovarajućih DNA segmenata. Ukupno je očitano 50 bendova, dužine fragmenata u rasponu od 500 do 3000 bp. Broj polimorfnih bendova po prajmeru se kretao od 8 do 13. U našem istraživanju na nivou svih analiziranih prajmera, utvrđen je visok stepen polimorfizma između analaziranih genotipova. Na osnovu UPGMA dendograma analizirani genotipovi su razvrstani u dva osnovna klastera i dva subklastera.sr
dc.publisherDruštvo genetičara Srbije, Beograd
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceGenetika
dc.subjectgenetic polymorphismen
dc.subjectRAPDen
dc.subjectonionen
dc.titleCharacterization of onion genotypes by use of RAPD markersen
dc.titleMogućnost primene molekularnih RAPD markera u selekciji crnog lukasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage278
dc.citation.issue2
dc.citation.other44(2): 269-278
dc.citation.rankM23
dc.citation.spage269
dc.citation.volume44
dc.description.other[http://www.dgsgenetika.org.rs/abstrakti/vol44_no2_rad6.pdf]
dc.identifier.doi10.2298/GENSR1202269P
dc.identifier.fulltexthttp://RIVeC.institut-palanka.rs/bitstream/id/17/117.pdf
dc.identifier.rcubconv_73
dc.identifier.scopus2-s2.0-84873458209
dc.identifier.wos000309073800006
dc.type.versionpublishedVersion


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