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Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)

Samo za registrovane korisnike
2013
Autori
Pavlović, Suzana
Vinterhalter, Branka
Zdravković-Korać, Snežana
Vinterhalter, Dragan
Zdravković, Jasmina
Cvikić, Dejan
Mitić, Nevena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A tot...al of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.

Ključne reči:
Cabbage / Cauliflower / Immature zygotic embryos / Recurrent somatic embryogenesis / Regeneration
Izvor:
Plant Cell Tissue and Organ Culture, 2013, 113, 3, 397-406
Izdavač:
  • Springer, Dordrecht
Finansiranje / projekti:
  • Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-173015)
  • Novi koncept oplemenjivanja sorti i hibrida povrća namenjenih održivim sistemima gajenja uz primenu biotehnoloških metoda (RS-31059)
Napomena:
  • https://link.springer.com/article/10.1007/s11240-012-0279-6

DOI: 10.1007/s11240-012-0279-6

ISSN: 0167-6857

WoS: 000319358000004

Scopus: 2-s2.0-84878180151
[ Google Scholar ]
22
18
URI
http://RIVeC.institut-palanka.rs/handle/123456789/143
Kolekcije
  • Radovi sa afilijacijom Instituta za povrtarstvo / Papers with the Institute for Vegetable Crops affiliation
Institucija/grupa
Institut za povrtarstvo
TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/143
AB  - A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)
EP  - 406
IS  - 3
SP  - 397
VL  - 113
DO  - 10.1007/s11240-012-0279-6
UR  - conv_171
ER  - 
@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Vinterhalter, Dragan and Zdravković, Jasmina and Cvikić, Dejan and Mitić, Nevena",
year = "2013",
abstract = "A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)",
pages = "406-397",
number = "3",
volume = "113",
doi = "10.1007/s11240-012-0279-6",
url = "conv_171"
}
Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Vinterhalter, D., Zdravković, J., Cvikić, D.,& Mitić, N.. (2013). Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 113(3), 397-406.
https://doi.org/10.1007/s11240-012-0279-6
conv_171
Pavlović S, Vinterhalter B, Zdravković-Korać S, Vinterhalter D, Zdravković J, Cvikić D, Mitić N. Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture. 2013;113(3):397-406.
doi:10.1007/s11240-012-0279-6
conv_171 .
Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Vinterhalter, Dragan, Zdravković, Jasmina, Cvikić, Dejan, Mitić, Nevena, "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)" in Plant Cell Tissue and Organ Culture, 113, no. 3 (2013):397-406,
https://doi.org/10.1007/s11240-012-0279-6 .,
conv_171 .

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