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dc.creatorPavlović, Suzana
dc.creatorVinterhalter, Branka
dc.creatorZdravković-Korać, Snežana
dc.creatorVinterhalter, Dragan
dc.creatorZdravković, Jasmina
dc.creatorCvikić, Dejan
dc.creatorMitić, Nevena
dc.date.accessioned2021-06-07T12:22:58Z
dc.date.available2021-06-07T12:22:58Z
dc.date.issued2013
dc.identifier.issn0167-6857
dc.identifier.urihttp://RIVeC.institut-palanka.rs/handle/123456789/143
dc.description.abstractA simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.en
dc.publisherSpringer, Dordrecht
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173015/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31059/RS//
dc.rightsrestrictedAccess
dc.sourcePlant Cell Tissue and Organ Culture
dc.subjectCabbageen
dc.subjectCaulifloweren
dc.subjectImmature zygotic embryosen
dc.subjectRecurrent somatic embryogenesisen
dc.subjectRegenerationen
dc.titleRecurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)en
dc.typearticle
dc.rights.licenseARR
dc.citation.epage406
dc.citation.issue3
dc.citation.other113(3): 397-406
dc.citation.rankM21
dc.citation.spage397
dc.citation.volume113
dc.description.other[https://link.springer.com/article/10.1007/s11240-012-0279-6]
dc.identifier.doi10.1007/s11240-012-0279-6
dc.identifier.rcubconv_171
dc.identifier.scopus2-s2.0-84878180151
dc.identifier.wos000319358000004
dc.type.versionpublishedVersion


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