Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia
2014
Аутори
Ugrinović, MilanMijatović, Mirjana
Zdravković, Jasmina
Girek, Zdenka
Kuzmanović, Đorđe
Rasulić, Nataša
Jošić, Dragana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants' yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)(5)-PCR fingerprinting showed pr...esence of six different patterns on the 92% similarity level.
Кључне речи:
Phaseolus vulgaris / Beta vulgaris / Raphanus sativus / PCR / mixed croppingИзвор:
Agricultural and Food Science, 2014, 23, 3, 173-185Издавач:
- Scientific Agricultural Soc Finland, Univ Helsinski
Финансирање / пројекти:
- Нови аутохтони изолати бактерија Lysobacter и Pseudomonas као важан извор метаболита корисних за биотехнологију, стимулацију раста биљака и контролу болести биља: од изолата до препарата (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46007)
- Нови концепт оплемењивања сорти и хибрида поврћа намењених одрживим системима гајења уз примену биотехнолошких метода (RS-MESTD-Technological Development (TD or TR)-31059)
DOI: 10.23986/afsci.41385
ISSN: 1459-6067
WoS: 000343597900003
Scopus: 2-s2.0-84939179602
Институција/група
Institut za povrtarstvoTY - JOUR AU - Ugrinović, Milan AU - Mijatović, Mirjana AU - Zdravković, Jasmina AU - Girek, Zdenka AU - Kuzmanović, Đorđe AU - Rasulić, Nataša AU - Jošić, Dragana PY - 2014 UR - http://RIVeC.institut-palanka.rs/handle/123456789/160 AB - The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants' yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)(5)-PCR fingerprinting showed presence of six different patterns on the 92% similarity level. PB - Scientific Agricultural Soc Finland, Univ Helsinski T2 - Agricultural and Food Science T1 - Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia EP - 185 IS - 3 SP - 173 VL - 23 DO - 10.23986/afsci.41385 ER -
@article{ author = "Ugrinović, Milan and Mijatović, Mirjana and Zdravković, Jasmina and Girek, Zdenka and Kuzmanović, Đorđe and Rasulić, Nataša and Jošić, Dragana", year = "2014", abstract = "The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants' yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)(5)-PCR fingerprinting showed presence of six different patterns on the 92% similarity level.", publisher = "Scientific Agricultural Soc Finland, Univ Helsinski", journal = "Agricultural and Food Science", title = "Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia", pages = "185-173", number = "3", volume = "23", doi = "10.23986/afsci.41385" }
Ugrinović, M., Mijatović, M., Zdravković, J., Girek, Z., Kuzmanović, Đ., Rasulić, N.,& Jošić, D.. (2014). Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia. in Agricultural and Food Science Scientific Agricultural Soc Finland, Univ Helsinski., 23(3), 173-185. https://doi.org/10.23986/afsci.41385
Ugrinović M, Mijatović M, Zdravković J, Girek Z, Kuzmanović Đ, Rasulić N, Jošić D. Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia. in Agricultural and Food Science. 2014;23(3):173-185. doi:10.23986/afsci.41385 .
Ugrinović, Milan, Mijatović, Mirjana, Zdravković, Jasmina, Girek, Zdenka, Kuzmanović, Đorđe, Rasulić, Nataša, Jošić, Dragana, "Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia" in Agricultural and Food Science, 23, no. 3 (2014):173-185, https://doi.org/10.23986/afsci.41385 . .