Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
2021
Authors
Belić, MajaZdravković-Korać, Snežana
Pavlović, Suzana
Živković, Suzana
Vinterhalter, Dragan
Ćalić, Dušica
Milojević, Jelena
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Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable ...for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
Keywords:
spinach / somatic embryogenesis / gibberellins / LED lights / gene expressionSource:
Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10, 2021, 213-213Publisher:
- East Sarajevo : Faculty of Agriculture
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Institut za povrtarstvoTY - CONF AU - Belić, Maja AU - Zdravković-Korać, Snežana AU - Pavlović, Suzana AU - Živković, Suzana AU - Vinterhalter, Dragan AU - Ćalić, Dušica AU - Milojević, Jelena PY - 2021 UR - http://RIVeC.institut-palanka.rs/handle/123456789/465 AB - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light. PB - East Sarajevo : Faculty of Agriculture C3 - Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 T1 - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L) EP - 213 SP - 213 UR - https://hdl.handle.net/21.15107/rcub_rivec_465 ER -
@conference{ author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena", year = "2021", abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.", publisher = "East Sarajevo : Faculty of Agriculture", journal = "Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10", title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)", pages = "213-213", url = "https://hdl.handle.net/21.15107/rcub_rivec_465" }
Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 East Sarajevo : Faculty of Agriculture., 213-213. https://hdl.handle.net/21.15107/rcub_rivec_465
Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10. 2021;:213-213. https://hdl.handle.net/21.15107/rcub_rivec_465 .
Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 (2021):213-213, https://hdl.handle.net/21.15107/rcub_rivec_465 .