Genetic determination of technological quality in Triticum durum
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Background and Purpose: This paper presents the results of an investigation of gliadin alleles variability and their relationship to technological quality components in 21 durum wheat cultivars. Material and Methods: Seeds of 21 durum cultivars were crashed and used for extraction of gliadins by 70% ethanols. At least 30 seeds wereusedforgliadinextraction. Thecompositionofgliadincomponents was analyzed by acid polyacrylamide gel electrophoresis (pH=3.1). Electrophoregrams wereusedforidentification of gliadin alleles. Technological quality of geneticaly divergent durum cultivars were evaluated by determination of wet gluten content and rheological (farinographic) dough properties. Results and Conclusion: Polymorphysms of alleles at each locus was registered. At four gliadin loci 27 different alleles were determined by analysis of 21durumcultivars.Eachcultivarhaddifferentgliadinallele composition. Frequency of each allele was computed and varied in the ratio from 4.8% to 42.9%. Values of... flour water absorption and gluten contents had on the level of B1 and B2 quality classes. Gliadin alleles at the Gli-B1 locus showed the highest positive connection with gluten contents. High frequency of alleles was related with good gluten quality and water absorption of flour. Gliadins can be used as a marker for biological traits of wheat.
Keywords:Alleles / Durum wheat quality / Electrophoresis / Frequency / Gliadins
Source:Periodicum Biologorum, 2008, 110, 3, 285-289
ISSN: 00315362 (ISSN)[ Google Scholar ]
Institution/CommunityInstitut za povrtarstvo
Đukić, N., Knežević, D.,& Zečević, V.. (2008). Genetic determination of technological quality in Triticum durum. in Periodicum Biologorum, 110(3), 285-289. https://hdl.handle.net/21.15107/rcub_rivec_476
Đukić N, Knežević D, Zečević V. Genetic determination of technological quality in Triticum durum. in Periodicum Biologorum. 2008;110(3):285-289. https://hdl.handle.net/21.15107/rcub_rivec_476 .
Đukić, Nevena, Knežević, Desimir, Zečević, Veselinka, "Genetic determination of technological quality in Triticum durum" in Periodicum Biologorum, 110, no. 3 (2008):285-289, https://hdl.handle.net/21.15107/rcub_rivec_476 .