Приказ основних података о документу
Introduction of resistance to herbicide Basta (R) in Savoy cabbage
dc.creator | Sretenović-Rajičić, Tatjana | |
dc.creator | Ninković, S. | |
dc.creator | Vinterhalter, Branka | |
dc.creator | Miljuš-Đukić, J | |
dc.creator | Vinterhalter, Dragan | |
dc.date.accessioned | 2021-06-07T12:16:59Z | |
dc.date.available | 2021-06-07T12:16:59Z | |
dc.date.issued | 2004 | |
dc.identifier.issn | 0006-3134 | |
dc.identifier.uri | http://RIVeC.institut-palanka.rs/handle/123456789/55 | |
dc.description.abstract | Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny. | en |
dc.publisher | Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6 | |
dc.rights | restrictedAccess | |
dc.source | Biologia Plantarum | |
dc.subject | Agrobacterium | en |
dc.subject | bar gene | en |
dc.subject | Brassica oleracea var. sabauda | en |
dc.subject | transformation | en |
dc.title | Introduction of resistance to herbicide Basta (R) in Savoy cabbage | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 436 | |
dc.citation.issue | 3 | |
dc.citation.other | 48(3): 431-436 | |
dc.citation.rank | M22 | |
dc.citation.spage | 431 | |
dc.citation.volume | 48 | |
dc.identifier.doi | 10.1023/B:BIOP.0000041098.18836.be | |
dc.identifier.scopus | 2-s2.0-4544320605 | |
dc.identifier.wos | 000223770500018 | |
dc.type.version | publishedVersion |