Efficiency of Serenade (Bacillus subtilis) in controlling Xanthomonas campestris pv. vesicatoria pepper pathogen

Abstract

Xanthomonas campestris pv. vesicatoria causes bacterial leaf spot of pepper. Control of this pathogen could be very significant, economically. During 2007, a trial has been set up at the experimental field at the Institute for Vegetable Crops, in order to investigate efficiency of preparation SERENADE® WP (Bacillus subtilis) and compare it to the copper preparations (standard). The researches have been performed on pepper variety PALANAČKA KAPIJA. Inoculation has been performed by using bacteria type KFB 4 (10ˉ8 cfu/mol). Preparations have been applied in following concentrations: SERENADE® WP (0, 3%) and (0, 4%), BLAUVIT (0, 5%), BORDO-BLUE (0.5%) and BAKROCID (0, 5%). Trial has been set with three replications and with following treatments: (a) Plants treated with preparations, and two hours later inoculated with bacteria suspension; (b) Plants inoculated firstly with bacteria suspension, and than, 4 hours later, with preparations; (c) Plants treated with preparations only; (d) Plants treated with bacteria suspension only; (e) Non-inoculated and non-treated plants. Inoculation has been performed in 8-10 leaves phoenophase, with hand sprayer. The first symptoms have been observed 7 days after inoculation, while preparation efficiency has been observed 10 days after inoculation, by testing 100 random leaves. Disease index has been set by Townsend-Heuberger model. The results show that SERENADE® WP was highly significant in both concentrations, comparing to standard copper preparations, both before (70.5% i.e. 78.6%) and after inoculation (75.2% i.e. 82.5%).

U cilju zaštite paprike od prouzrokovača bakteriozne pegavosti lišća paprike (Xanthomonas campestris pv. vesicatoria), tokom 2007. godine postavljen je ogled na polju Instituta u Smederevskoj Palanci sa ciljem da se ispita efikasnost biološkog preparata Serenade WP (Bacillus subtilis) u odnosu na preparate na bazi bakra kao standard. Ispitivanja su obavljena na sorti Palanačka kapija. Za inokulaciju korišćen je soj bakterije KFB 4 (10ˉ8 cfu/mol). Preparati su primenjeni u sledećim koncentarcijama: Serenade WP(0,3%) i (0,4%), Blauvit (0,5%), Bordo-blue (0.5%) i Bakrocid S-50 (0,5%). Ogled je postavljen u tri ponavljanja sa sledećim tretmanima: (a) biljke tretirane preparatima, a nakon 2 sata inokulisane suspenzijom bakterija; (b) biljke inokulisane suspenzijom bakterija a posle 4 sata tretirane preparatima; (c) biljke tretirane samo preparatima; (d) biljke tretirane samo suspenzijom bakterija; (e) ne inokulisane i netretirane biljke. Inokulacija je vršena u fenofazi 8-10 listova ručnom prskalicom. Konstantovanje prvih simptoma izvršeno je 7 dana nakon inokulacije. Ocena efikasnosti preparata 10 dana posle inokulacije, uzimanjem 100 listova po slučajnom izboru. Indeks oboljenja ocenjen je po Townsend-Heuberger-u. Na osnovu dobijenih rezultata zaključujemo da je preparat Serenade pokazao visok stepen efikasnosti u obe koncentracije, u odnosu na standardne bakarne preparate, kako pre (70.5%, odnosno 78.6%) tako i nakon inokulacije (75.2%, odnosno 82.5%).