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Effect of tobacco protein kinase 1 (NPK1) gene expression in transformed cauliflower (Brassica oleracea L. var. botrytis) plants on the development of tolerance to increased concentrations of NaCl in vitro

dc.contributor.advisorMitić, Nevena
dc.contributor.otherJanošević, Dušica
dc.contributor.otherVinterhalter, Branka
dc.contributor.otherSavić, Jelena
dc.contributor.otherCvetić-Antić, Tijana
dc.creatorPavlović, Suzana
dc.date.accessioned2021-06-07T12:11:17Z
dc.date.available2021-06-07T12:11:17Z
dc.date.issued2015
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/4622
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=2449
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:10460/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47614991
dc.identifier.urihttp://RIVeC.institut-palanka.rs/handle/123456789/7
dc.description.abstractBiljke su neprekidno izloţene razliĉitim vrstama stresa iz spoljašnje sredine, koji nepovoljno utiĉu na njihov rast i razviće. Povećan salinitet zemljišta je jedan od faktora spoljašnje sredine, koji u velikoj meri ograniĉava produktivnost gajenih biljaka. Karfiol (Brassica oleracea L. var. botrytis), je znaĉajna povrtarska kultura koja je, kao i ostale povrtarske kupusnjaĉe, podloţna uticaju razliĉitih stresnih faktora, pa i povećanom salinitetu usled intenzivnog zalivanja. Uz klasiĉne metode oplemenjivanja, biotehnološke metode mogu biti efikasan pristup za povećanje tolerancije biljaka prema stresu. Mitogenom aktivirane protein-kinaze (MAPK) igraju kljuĉnu ulogu u rastu i razviću biljaka i njihovom odgovoru na biotiĉke i abiotiĉke faktore spoljašnje sredine. Introdukcija gena za komponente MAPK kaskada moţe dovesti do razvoja transgenih biljaka sa povećanom tolerancijom prema stresu, pri ĉemu su MAPKKK najpogodnije za genetiĉki inţenjering jer funkcionišu na poĉetku kaskade koja vodi aktivaciji više transkripcionih faktora i stres inducibilnih gena. Preduslov za primenu ovih metoda u oplemenjivanju biljaka jeste uspostavljanje efikasnog sistema za regeneraciju biljaka in vitro. Kod ĉetiri varijeteta B. oleracea (karfiola, kupusa, brokolija i kelja) uspostavljen je sistem za regeneraciju pupoljaka in vitro putem organogeneze. Eksplantati hipokotila su kod skoro svih testiranih varijeteta imali najbolji regenerativni potencijal (od 75,0-91,2% sa produkcijom 3,5-7,4 pupoljaka po eksplantatu). Podloge koje su sadrţale N6-benziladenin (BA) su bile optimalne, kako za regeneraciju pupoljaka, tako i za njihovu kasniju multiplikaciju. Regenerisani izdanci su uspešno oţiljeni i aklimatizovani. Kod kupusa i karfiola uspostavljen je i jednostavan protokol za direktnu somatsku embriogenezu iz nezrelih zigotskih embriona gajenih na B5 podlozi bez regulatora rastenja (B5-0), koji je omogućio efikasnu transformaciju karfiola kao, u tom pogledu, problematiĉnog varijeteta. Kod oba varijeteta, zigotski embrioni u kotiledonarnom stadijumu razvoja imali su najveći kapacitet za formiranje somatskih embriona (11,84 kod kupusa i 11,95 kod karfiola)...sr
dc.description.abstractPlants are continuously exposed to various types of stress from the environment that adversely affect their growth and development. The increased salinity of the soil is one of the environmetal factors that greatly limits the productivity of the cultivated plants. Cauliflower (Brassica oleracea L. var. botrytis), is an important vegetable crop, which, like other Brassica vegetable, is grown under the influence of various biotic and abiotic stresses, including the increased salinity due to intensive watering. In addition to classical breeding methods, biotechnology methods may be an effective approach to increase plants stress tolerance. The mitogen-activated protein kinases (MAPK) play a key role in the growth and development of plants and their response to biotic and abiotic stresses. Introduction of genes for components of MAPK cascade may lead to the development of transgenic plants with the increased tolerance to stress, wherein MAPKKK are most suitable for genetic engineering, because they act at the beginning of the cascade that leads to activation of several transcription factors and stress inducible genes. A prerequisite for the application of these methods in plant breeding is establishing an efficient plant regeneration system in vitro. A regenerative system via organogenesis was established in four B. oleracea varieties (cauliflower, cabbage, broccoli, kale). The hypocotyl explants in almost all the tested varieties have the best regenerative potential (from 75.0-91.2% with the production of 3.5-7.4 shoots per explant). The media containing N6-benzyladenine (BA) were optimal both for shoot regeneration and their subsequent multiplication. Regenerated shoots were succesfully rooted and acclimatized. In cabbage and cauliflower a simple protocol was established for the direct somatic embryogenesis from immature zigotic embryos grown on B5 medium without growth regulators (B5-0), which enabled the efficient transformation of cauliflower, in this regard, as a problematic variety. In both varieties, zygotic embryos at the cotyledonary stage of development had the highest embryo-forming capacity (11.84 in cabbage and 11.95 in cauliflower)...en
dc.languagesr
dc.publisherUniverzitet u Beogradu, Biološki fakultet
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31059/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectBrassica oleracea L.sr
dc.subjectBrassica oleracea L.en
dc.subjectkarfiolsr
dc.subjectorganogenezasr
dc.subjectsomatska embriogenezasr
dc.subjectgenetiĉka transformacijasr
dc.subjectAgrobacterium tumefacienssr
dc.subjectNPK1 gensr
dc.subjectbar gensr
dc.subjectabiotiĉki stressr
dc.subjectsalinitetsr
dc.subjectcaulifloweren
dc.subjectorganogenesisen
dc.subjectsomatic embryogenesisen
dc.subjectgenetic transformationen
dc.subjectAgrobacterium tumefaciensen
dc.subjectNPK1 geneen
dc.subjectbar geneen
dc.subjectabiotic stressen
dc.subjectsalinityen
dc.titleEfekat ekspresije gena za protein-kinazu 1 duvana (NPK1) kod transformisanih biljaka karfiola (Brassica oleracea L. var. botrytis) na razvoj tolerancije prema povišenim koncentracijama NaCI in vitrosr
dc.titleEffect of tobacco protein kinase 1 (NPK1) gene expression in transformed cauliflower (Brassica oleracea L. var. botrytis) plants on the development of tolerance to increased concentrations of NaCl in vitroen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-SA
dc.identifier.fulltexthttp://RIVeC.institut-palanka.rs/bitstream/id/134/4.pdf
dc.identifier.rcubt-492
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_4622
dc.type.versionpublishedVersion


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