Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids
2021
Autori
Živković, IvanaSavikin, Katarina
Živković, Jelena
Zdunić, Gordana
Janković, Teodora
Lazić, Dejan
Radin, Dragoslava
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. ...A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.
Ključne reči:
Human norovirus / Pomegranate peel extract / Antiviral effect / Food modelIzvor:
Plant Foods for Human Nutrition, 2021Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200116 (Univerzitet u Beogradu, Poljoprivredni fakultet) (RS-MESTD-inst-2020-200116)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200003 (Institut za proučavanje lekovitog bilja 'Dr Josif Pančić', Beograd) (RS-MESTD-inst-2020-200003)
DOI: 10.1007/s11130-021-00895-7
ISSN: 0921-9668
PubMed: 33825092
WoS: 000637457100002
Scopus: 2-s2.0-85103623738
Institucija/grupa
Institut za povrtarstvoTY - JOUR AU - Živković, Ivana AU - Savikin, Katarina AU - Živković, Jelena AU - Zdunić, Gordana AU - Janković, Teodora AU - Lazić, Dejan AU - Radin, Dragoslava PY - 2021 UR - http://RIVeC.institut-palanka.rs/handle/123456789/11 AB - Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses. PB - Springer, Dordrecht T2 - Plant Foods for Human Nutrition T1 - Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids DO - 10.1007/s11130-021-00895-7 ER -
@article{ author = "Živković, Ivana and Savikin, Katarina and Živković, Jelena and Zdunić, Gordana and Janković, Teodora and Lazić, Dejan and Radin, Dragoslava", year = "2021", abstract = "Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.", publisher = "Springer, Dordrecht", journal = "Plant Foods for Human Nutrition", title = "Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids", doi = "10.1007/s11130-021-00895-7" }
Živković, I., Savikin, K., Živković, J., Zdunić, G., Janković, T., Lazić, D.,& Radin, D.. (2021). Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids. in Plant Foods for Human Nutrition Springer, Dordrecht.. https://doi.org/10.1007/s11130-021-00895-7
Živković I, Savikin K, Živković J, Zdunić G, Janković T, Lazić D, Radin D. Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids. in Plant Foods for Human Nutrition. 2021;. doi:10.1007/s11130-021-00895-7 .
Živković, Ivana, Savikin, Katarina, Živković, Jelena, Zdunić, Gordana, Janković, Teodora, Lazić, Dejan, Radin, Dragoslava, "Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids" in Plant Foods for Human Nutrition (2021), https://doi.org/10.1007/s11130-021-00895-7 . .