Allelic composition of HMW-glutenin protein and their relationship with quality of wheat
Authors
Knežević, DesimirNovoselskaya Dragovich, Aleksandra Yu
Kudryavcev, Aleksander
Kondić, Danijela
Branković, Gordana
Srdić, Sretenka
Zečević, Veselinka
Article (Published version)
Metadata
Show full item recordAbstract
High molecular weight glutenin subunits (HMW-GS) proteins deposited in
endosperm of wheat seed which have significant impact on bread quality. The
HMW-GS encoded by genes located at the long arm of chromosomes 1A, 1B, 1D.
The aim of this work was study allele polymorphysms at Glu-A1, Glu-B1 and GluD1 locus and loaf volume, grain protein content, sedimentation volume of eight
wheat genotypes (G-3130, G-35183, G-3501, G3512, G-3574, G-3027, G-3075, G3097) harvested in two years with various weather condition. For each genotypes,
flour used for extraction of glutenin which separated by method of electrophoresis
on SDS gel (11.8%). Electrophoregrams used for determining Glu-1 alleles.
Technological quality parameters analyzed by standard laboratory methods. The
three alleles alleles (a, b, c) at the Glu-A1, three alleles (b, c, d) at the Glu-B1 and 2
alleles (a, d) at the Glu-D1 were identified. The highest protein sedimentation
volume had wheat genotype G-3075 in the both years ...(54.0ml; 58.0ml) while the
lowest sedimentation volume had G-3512 (34.0ml; 36.0ml). Grain protein content
(GPC) was the highest in G-3075 in both years (14.20%; 15.40%) while the lowest
GPC had G-3097 (11.60%) in first and G-3512 (12.60%) in the second year. Loaf
volume was the highest in G-3075 in both year (520ml; 540ml) while the lowest
was in G-3512 (400ml) in both years of experiment. The estimated quality traits
varied depending on genotype and year. The better quality, in average, had the
wheat genotypes which carried Glu-D1d allele.
Keywords:
wheat / glutenin / glu-1 allele / quality / polymorphismSource:
Agrofor International Journal, 2018, 3, 2, 14-21Publisher:
- University of East Sarajevo, Faculty of Agriculture
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Institut za povrtarstvoTY - JOUR AU - Knežević, Desimir AU - Novoselskaya Dragovich, Aleksandra Yu AU - Kudryavcev, Aleksander AU - Kondić, Danijela AU - Branković, Gordana AU - Srdić, Sretenka AU - Zečević, Veselinka PY - 2018 UR - http://RIVeC.institut-palanka.rs/handle/123456789/549 AB - High molecular weight glutenin subunits (HMW-GS) proteins deposited in endosperm of wheat seed which have significant impact on bread quality. The HMW-GS encoded by genes located at the long arm of chromosomes 1A, 1B, 1D. The aim of this work was study allele polymorphysms at Glu-A1, Glu-B1 and GluD1 locus and loaf volume, grain protein content, sedimentation volume of eight wheat genotypes (G-3130, G-35183, G-3501, G3512, G-3574, G-3027, G-3075, G3097) harvested in two years with various weather condition. For each genotypes, flour used for extraction of glutenin which separated by method of electrophoresis on SDS gel (11.8%). Electrophoregrams used for determining Glu-1 alleles. Technological quality parameters analyzed by standard laboratory methods. The three alleles alleles (a, b, c) at the Glu-A1, three alleles (b, c, d) at the Glu-B1 and 2 alleles (a, d) at the Glu-D1 were identified. The highest protein sedimentation volume had wheat genotype G-3075 in the both years (54.0ml; 58.0ml) while the lowest sedimentation volume had G-3512 (34.0ml; 36.0ml). Grain protein content (GPC) was the highest in G-3075 in both years (14.20%; 15.40%) while the lowest GPC had G-3097 (11.60%) in first and G-3512 (12.60%) in the second year. Loaf volume was the highest in G-3075 in both year (520ml; 540ml) while the lowest was in G-3512 (400ml) in both years of experiment. The estimated quality traits varied depending on genotype and year. The better quality, in average, had the wheat genotypes which carried Glu-D1d allele. PB - University of East Sarajevo, Faculty of Agriculture T2 - Agrofor International Journal T1 - Allelic composition of HMW-glutenin protein and their relationship with quality of wheat EP - 21 IS - 2 SP - 14 VL - 3 VL - 21 DO - 10.7251/AGRENG1802014K ER -
@article{ author = "Knežević, Desimir and Novoselskaya Dragovich, Aleksandra Yu and Kudryavcev, Aleksander and Kondić, Danijela and Branković, Gordana and Srdić, Sretenka and Zečević, Veselinka", year = "2018", abstract = "High molecular weight glutenin subunits (HMW-GS) proteins deposited in endosperm of wheat seed which have significant impact on bread quality. The HMW-GS encoded by genes located at the long arm of chromosomes 1A, 1B, 1D. The aim of this work was study allele polymorphysms at Glu-A1, Glu-B1 and GluD1 locus and loaf volume, grain protein content, sedimentation volume of eight wheat genotypes (G-3130, G-35183, G-3501, G3512, G-3574, G-3027, G-3075, G3097) harvested in two years with various weather condition. For each genotypes, flour used for extraction of glutenin which separated by method of electrophoresis on SDS gel (11.8%). Electrophoregrams used for determining Glu-1 alleles. Technological quality parameters analyzed by standard laboratory methods. The three alleles alleles (a, b, c) at the Glu-A1, three alleles (b, c, d) at the Glu-B1 and 2 alleles (a, d) at the Glu-D1 were identified. The highest protein sedimentation volume had wheat genotype G-3075 in the both years (54.0ml; 58.0ml) while the lowest sedimentation volume had G-3512 (34.0ml; 36.0ml). Grain protein content (GPC) was the highest in G-3075 in both years (14.20%; 15.40%) while the lowest GPC had G-3097 (11.60%) in first and G-3512 (12.60%) in the second year. Loaf volume was the highest in G-3075 in both year (520ml; 540ml) while the lowest was in G-3512 (400ml) in both years of experiment. The estimated quality traits varied depending on genotype and year. The better quality, in average, had the wheat genotypes which carried Glu-D1d allele.", publisher = "University of East Sarajevo, Faculty of Agriculture", journal = "Agrofor International Journal", title = "Allelic composition of HMW-glutenin protein and their relationship with quality of wheat", pages = "21-14", number = "2", volume = "3, 21", doi = "10.7251/AGRENG1802014K" }
Knežević, D., Novoselskaya Dragovich, A. Y., Kudryavcev, A., Kondić, D., Branković, G., Srdić, S.,& Zečević, V.. (2018). Allelic composition of HMW-glutenin protein and their relationship with quality of wheat. in Agrofor International Journal University of East Sarajevo, Faculty of Agriculture., 3(2), 14-21. https://doi.org/10.7251/AGRENG1802014K
Knežević D, Novoselskaya Dragovich AY, Kudryavcev A, Kondić D, Branković G, Srdić S, Zečević V. Allelic composition of HMW-glutenin protein and their relationship with quality of wheat. in Agrofor International Journal. 2018;3(2):14-21. doi:10.7251/AGRENG1802014K .
Knežević, Desimir, Novoselskaya Dragovich, Aleksandra Yu, Kudryavcev, Aleksander, Kondić, Danijela, Branković, Gordana, Srdić, Sretenka, Zečević, Veselinka, "Allelic composition of HMW-glutenin protein and their relationship with quality of wheat" in Agrofor International Journal, 3, no. 2 (2018):14-21, https://doi.org/10.7251/AGRENG1802014K . .