TY  - JOUR
AU  - Pavlović, Suzana
AU  - Savić, Jelena
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Girek, Zdenka
AU  - Adžić, Slađan
AU  - Zečević, Bogoljub
AU  - Banjac, Nevena
PY  - 2020
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/215
AB  - Key message The results underlined that constitutively expressingNPK1can significantly contribute to enhanced salt stress tolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerance cruciferous vegetable lines. Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression ofNPK1transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. TheAgrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring theNPK1and phosphinothricinN-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of bothNPK1andbartransgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level ofNPK1gene expression estimated by RT-qPCR, and the L2 line with the highestNPK1expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of thebargene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
EP  - 651
IS  - 3
SP  - 635
VL  - 143
DO  - 10.1007/s11240-020-01948-6
ER  - 

@article{
author = "Pavlović, Suzana and Savić, Jelena and Milojević, Jelena and Vinterhalter, Branka and Girek, Zdenka and Adžić, Slađan and Zečević, Bogoljub and Banjac, Nevena",
year = "2020",
abstract = "Key message The results underlined that constitutively expressingNPK1can significantly contribute to enhanced salt stress tolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerance cruciferous vegetable lines. Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression ofNPK1transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. TheAgrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring theNPK1and phosphinothricinN-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of bothNPK1andbartransgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level ofNPK1gene expression estimated by RT-qPCR, and the L2 line with the highestNPK1expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of thebargene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower",
pages = "651-635",
number = "3",
volume = "143",
doi = "10.1007/s11240-020-01948-6"
}

Pavlović, S., Savić, J., Milojević, J., Vinterhalter, B., Girek, Z., Adžić, S., Zečević, B.,& Banjac, N.. (2020). Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 143(3), 635-651.
https://doi.org/10.1007/s11240-020-01948-6

Pavlović S, Savić J, Milojević J, Vinterhalter B, Girek Z, Adžić S, Zečević B, Banjac N. Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell Tissue and Organ Culture. 2020;143(3):635-651.
doi:10.1007/s11240-020-01948-6 .

Pavlović, Suzana, Savić, Jelena, Milojević, Jelena, Vinterhalter, Branka, Girek, Zdenka, Adžić, Slađan, Zečević, Bogoljub, Banjac, Nevena, "Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower" in Plant Cell Tissue and Organ Culture, 143, no. 3 (2020):635-651,
https://doi.org/10.1007/s11240-020-01948-6 . .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/143
AB  - A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)
EP  - 406
IS  - 3
SP  - 397
VL  - 113
DO  - 10.1007/s11240-012-0279-6
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Vinterhalter, Dragan and Zdravković, Jasmina and Cvikić, Dejan and Mitić, Nevena",
year = "2013",
abstract = "A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)",
pages = "406-397",
number = "3",
volume = "113",
doi = "10.1007/s11240-012-0279-6"
}

Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Vinterhalter, D., Zdravković, J., Cvikić, D.,& Mitić, N.. (2013). Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 113(3), 397-406.
https://doi.org/10.1007/s11240-012-0279-6

Pavlović S, Vinterhalter B, Zdravković-Korać S, Vinterhalter D, Zdravković J, Cvikić D, Mitić N. Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture. 2013;113(3):397-406.
doi:10.1007/s11240-012-0279-6 .

Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Vinterhalter, Dragan, Zdravković, Jasmina, Cvikić, Dejan, Mitić, Nevena, "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)" in Plant Cell Tissue and Organ Culture, 113, no. 3 (2013):397-406,
https://doi.org/10.1007/s11240-012-0279-6 . .

TY  - CONF
AU  - Pavlović, Suzana
AU  - Adžić, Slađan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/139
AB  - An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.
EP  - 246
SP  - 239
VL  - 1005
DO  - 10.17660/ActaHortic.2013.1005.26
ER  - 

@conference{
author = "Pavlović, Suzana and Adžić, Slađan and Zdravković, Jasmina and Cvikić, Dejan and Milojević, Jelena and Vinterhalter, Branka and Mitić, Nevena",
year = "2013",
abstract = "An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.",
pages = "246-239",
volume = "1005",
doi = "10.17660/ActaHortic.2013.1005.26"
}

Pavlović, S., Adžić, S., Zdravković, J., Cvikić, D., Milojević, J., Vinterhalter, B.,& Mitić, N.. (2013). Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae
International Society for Horticultural Science., 1005, 239-246.
https://doi.org/10.17660/ActaHortic.2013.1005.26

Pavlović S, Adžić S, Zdravković J, Cvikić D, Milojević J, Vinterhalter B, Mitić N. Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae. 2013;1005:239-246.
doi:10.17660/ActaHortic.2013.1005.26 .

Pavlović, Suzana, Adžić, Slađan, Zdravković, Jasmina, Cvikić, Dejan, Milojević, Jelena, Vinterhalter, Branka, Mitić, Nevena, "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L." in Acta Horticulturae, 1005 (2013):239-246,
https://doi.org/10.17660/ActaHortic.2013.1005.26 . .

TY  - JOUR
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Pavlović, Suzana
AU  - Mitić, Nevena
AU  - Calić, Dušica
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/123
AB  - As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Long days promote somatic embryogenesis in spinach
EP  - 37
SP  - 32
VL  - 142
DO  - 10.1016/j.scienta.2012.04.020
ER  - 

@article{
author = "Milojević, Jelena and Tubić, Ljiljana and Pavlović, Suzana and Mitić, Nevena and Calić, Dušica and Vinterhalter, Branka and Zdravković-Korać, Snežana",
year = "2012",
abstract = "As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Long days promote somatic embryogenesis in spinach",
pages = "37-32",
volume = "142",
doi = "10.1016/j.scienta.2012.04.020"
}

Milojević, J., Tubić, L., Pavlović, S., Mitić, N., Calić, D., Vinterhalter, B.,& Zdravković-Korać, S.. (2012). Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 142, 32-37.
https://doi.org/10.1016/j.scienta.2012.04.020

Milojević J, Tubić L, Pavlović S, Mitić N, Calić D, Vinterhalter B, Zdravković-Korać S. Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae. 2012;142:32-37.
doi:10.1016/j.scienta.2012.04.020 .

Milojević, Jelena, Tubić, Ljiljana, Pavlović, Suzana, Mitić, Nevena, Calić, Dušica, Vinterhalter, Branka, Zdravković-Korać, Snežana, "Long days promote somatic embryogenesis in spinach" in Scientia Horticulturae, 142 (2012):32-37,
https://doi.org/10.1016/j.scienta.2012.04.020 . .

TY  - CONF
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Adžić, Slađan
AU  - Đorđević, Mladen
AU  - Ugrinović, Milan
AU  - Mitić, Nevena
PY  - 2011
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/577
AB  - Cauliflower (Brassica oleracea var. botrytis) is an important vegetable plant grown worldwide that is impacted by biotic and abiotic stresses. Plant biotechnology could be an efficient approach to enhance stress tolerance. Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as biotic and abiotic stress response. Thus, we have established an Agrobaterium tumefacens-mediated protocol for transformation of cauliflower (line KFRM4) using the binary vector pSHX004 with the mitogen-activated protein kinase kinase kinase (MAPKKK) gene from tobacco (NPK1) for stress tolerance and the phosphinothricin acetyltransferase (bar) selectable marker gene for herbicide resistance. The effect of acetosyringone (AS) on transformation efficiency was also investigated. Hypocotyls of 8 day-old seedlings, pre-cultivated on MS shoot induction medium (SIM) supplemented with 1.0 mg/l BA and 0.2 mg/l IBA for 3 days, were infected with bacterial suspension containing 0 or 100 μM AS and than co-cultivated on SIM with the same concentration of AS as infection medium for 3 days. Upon co-cultivation, the explants were cultured on SIM containing  500 mg/l cefotaxime for 8 days, and than subcultured on SIM selection media with 5 mg/l phosphinothricin (PPT) until shoot regeneration. The addition of AS during co-cultivation increased transformation frequency (7.8% vs. 1.4% in its absence). Selected PPT-resistant shoots were continuously maintained and multiplied on MS selection medium containing 2 mg/l PPT and BA or Kin (0.5-1.0 mg/l) alone or in combination with IBA (0.1-0.2 mg/l) displaying vigorous growth and development. Integration of the bar gene in transgenic shoots was verified by chlorophenol red (CR) assay while PCR confirmed the presence of NPK1 gene. Transformed plants provide a promising material for stress tolerance study.
PB  - Beograd : Serbian Plant Physiology Society
PB  - Beograd : Institute for Biological Research "Siniša Stanković"
C3  - 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts
T1  - Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_rivec_577
ER  - 

@conference{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Adžić, Slađan and Đorđević, Mladen and Ugrinović, Milan and Mitić, Nevena",
year = "2011",
abstract = "Cauliflower (Brassica oleracea var. botrytis) is an important vegetable plant grown worldwide that is impacted by biotic and abiotic stresses. Plant biotechnology could be an efficient approach to enhance stress tolerance. Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as biotic and abiotic stress response. Thus, we have established an Agrobaterium tumefacens-mediated protocol for transformation of cauliflower (line KFRM4) using the binary vector pSHX004 with the mitogen-activated protein kinase kinase kinase (MAPKKK) gene from tobacco (NPK1) for stress tolerance and the phosphinothricin acetyltransferase (bar) selectable marker gene for herbicide resistance. The effect of acetosyringone (AS) on transformation efficiency was also investigated. Hypocotyls of 8 day-old seedlings, pre-cultivated on MS shoot induction medium (SIM) supplemented with 1.0 mg/l BA and 0.2 mg/l IBA for 3 days, were infected with bacterial suspension containing 0 or 100 μM AS and than co-cultivated on SIM with the same concentration of AS as infection medium for 3 days. Upon co-cultivation, the explants were cultured on SIM containing  500 mg/l cefotaxime for 8 days, and than subcultured on SIM selection media with 5 mg/l phosphinothricin (PPT) until shoot regeneration. The addition of AS during co-cultivation increased transformation frequency (7.8% vs. 1.4% in its absence). Selected PPT-resistant shoots were continuously maintained and multiplied on MS selection medium containing 2 mg/l PPT and BA or Kin (0.5-1.0 mg/l) alone or in combination with IBA (0.1-0.2 mg/l) displaying vigorous growth and development. Integration of the bar gene in transgenic shoots was verified by chlorophenol red (CR) assay while PCR confirmed the presence of NPK1 gene. Transformed plants provide a promising material for stress tolerance study.",
publisher = "Beograd : Serbian Plant Physiology Society, Beograd : Institute for Biological Research "Siniša Stanković"",
journal = "1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts",
title = "Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_rivec_577"
}

Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Adžić, S., Đorđević, M., Ugrinović, M.,& Mitić, N.. (2011). Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance. in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts
Beograd : Serbian Plant Physiology Society., 64.
https://hdl.handle.net/21.15107/rcub_rivec_577

Pavlović S, Vinterhalter B, Zdravković-Korać S, Adžić S, Đorđević M, Ugrinović M, Mitić N. Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance. in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts. 2011;:64.
https://hdl.handle.net/21.15107/rcub_rivec_577 .

Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Adžić, Slađan, Đorđević, Mladen, Ugrinović, Milan, Mitić, Nevena, "Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance" in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts (2011):64,
https://hdl.handle.net/21.15107/rcub_rivec_577 .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Mitić, Nevena
AU  - Adžić, Slađan
AU  - Pavlović, Nenad
AU  - Zdravković, Milan
AU  - Vinterhalter, Dragan
PY  - 2010
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/86
AB  - Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse.
AB  - Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower
T1  - Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola
EP  - 345
IS  - 2
SP  - 337
VL  - 62
DO  - 10.2298/ABS1002337P
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Mitić, Nevena and Adžić, Slađan and Pavlović, Nenad and Zdravković, Milan and Vinterhalter, Dragan",
year = "2010",
abstract = "Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse., Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower, Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola",
pages = "345-337",
number = "2",
volume = "62",
doi = "10.2298/ABS1002337P"
}

Pavlović, S., Vinterhalter, B., Mitić, N., Adžić, S., Pavlović, N., Zdravković, M.,& Vinterhalter, D.. (2010). In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 337-345.
https://doi.org/10.2298/ABS1002337P

Pavlović S, Vinterhalter B, Mitić N, Adžić S, Pavlović N, Zdravković M, Vinterhalter D. In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower. in Archives of Biological Sciences. 2010;62(2):337-345.
doi:10.2298/ABS1002337P .

Pavlović, Suzana, Vinterhalter, Branka, Mitić, Nevena, Adžić, Slađan, Pavlović, Nenad, Zdravković, Milan, Vinterhalter, Dragan, "In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower" in Archives of Biological Sciences, 62, no. 2 (2010):337-345,
https://doi.org/10.2298/ABS1002337P . .

TY  - CONF
AU  - Pavlović, Suzana
AU  - Adžić, Slađan
AU  - Vinterhalter, Branka
PY  - 2009
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/578
AB  - Kod šest kultivara podvrsta kupusa, brokolija, kelja i karfiola vrste Brassica oleracea  istražena je sposobnost in vitro regeneracije pupoljaka. Segmenti  kotiledona, hipokotila i korena klijanaca starih 7 dana korišćeni su kao eksplantati. Inkubiran su na Murashige i Skoog (MS) podlogu sa različitim kombinacijama auksina i citokinina (BAP + IBA, BAP + NAA, KIN + IBA). Najviši procenat regenerisanih pupoljaka je dobijen na hipokotilima, dok su kotiledoni uglavnom produkovali korenove. Sposobnost regeneracije široko je varirala zavisno od kultivara, tipa eksplantata i koncentracije primenjene hormonalne kombinacije. Za kupuse najbolja regeneracija pupoljaka bila je na MS podlozi sa 1,0 mg/l BAP i 0,5 mg/l IBA, dok je kod brokolija i kelja to MS medijum sa 1.0 mg/l KIN  i 0.2 mg/l IBA. Najviše kalusa i vitrifikovanih pupoljaka razvijalo se na podlozi sa 1,0 mg/l BAP + 0,2 mg/l NAA kod svih 6 ispitivanih kultivara.
AB  - Six cultivars of  Brassica oleracea subspecies (cabbage, broccoli, kale and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root segments of 7-day-old seedlings were used as explants. They were incubated on Murashige & Skoog (MS) medium supplemented with different combinations of cytokinins and auxines (BAP + IBA, BAP + NAA, KIN + IBA). Hypocotyls were the most responsive for shoot regeneration and cotyledons for root regeneration. Regeneration ability widely varied depending on cultivar, explant type and concentration of growth regulators. For cabbage the best shoot regeneration was observed on MS medium supplemented with 1.0 mgl -1 BA and 0.5 mgl-1 IBA, while for broccoli and kale the highest shoot regeneration was on MS medium supplemented with 1.0 mgl -1 KIN and 0.2 mgl-1 IBA. Callus formation in all investigated cultivars was most pronounced on MS medium with 1.0 mg/l BA + 0.2 mg/l NAA.
PB  - Beograd : Društvo za fiziologiju biljaka Srbije
PB  - Beograd  : Institut za biološka istraživanja "Siniša Stanković"
C3  - Program i izvodi saopštenja : XVIII simpozijum Društva za fiziologiju biljaka Srbije, Vršac, 25-27. maj
T1  - In vitro regeneracija pupoljaka iz segmenata kotiledona, hipokotila i  korena kod Brassica oleraceae ssp.
T1  - In vitro shoot regeneration from cotyledon, hypocotyl and root explants  in  Brassica oleraceae ssp.
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rivec_578
ER  - 

@conference{
author = "Pavlović, Suzana and Adžić, Slađan and Vinterhalter, Branka",
year = "2009",
abstract = "Kod šest kultivara podvrsta kupusa, brokolija, kelja i karfiola vrste Brassica oleracea  istražena je sposobnost in vitro regeneracije pupoljaka. Segmenti  kotiledona, hipokotila i korena klijanaca starih 7 dana korišćeni su kao eksplantati. Inkubiran su na Murashige i Skoog (MS) podlogu sa različitim kombinacijama auksina i citokinina (BAP + IBA, BAP + NAA, KIN + IBA). Najviši procenat regenerisanih pupoljaka je dobijen na hipokotilima, dok su kotiledoni uglavnom produkovali korenove. Sposobnost regeneracije široko je varirala zavisno od kultivara, tipa eksplantata i koncentracije primenjene hormonalne kombinacije. Za kupuse najbolja regeneracija pupoljaka bila je na MS podlozi sa 1,0 mg/l BAP i 0,5 mg/l IBA, dok je kod brokolija i kelja to MS medijum sa 1.0 mg/l KIN  i 0.2 mg/l IBA. Najviše kalusa i vitrifikovanih pupoljaka razvijalo se na podlozi sa 1,0 mg/l BAP + 0,2 mg/l NAA kod svih 6 ispitivanih kultivara., Six cultivars of  Brassica oleracea subspecies (cabbage, broccoli, kale and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root segments of 7-day-old seedlings were used as explants. They were incubated on Murashige & Skoog (MS) medium supplemented with different combinations of cytokinins and auxines (BAP + IBA, BAP + NAA, KIN + IBA). Hypocotyls were the most responsive for shoot regeneration and cotyledons for root regeneration. Regeneration ability widely varied depending on cultivar, explant type and concentration of growth regulators. For cabbage the best shoot regeneration was observed on MS medium supplemented with 1.0 mgl -1 BA and 0.5 mgl-1 IBA, while for broccoli and kale the highest shoot regeneration was on MS medium supplemented with 1.0 mgl -1 KIN and 0.2 mgl-1 IBA. Callus formation in all investigated cultivars was most pronounced on MS medium with 1.0 mg/l BA + 0.2 mg/l NAA.",
publisher = "Beograd : Društvo za fiziologiju biljaka Srbije, Beograd  : Institut za biološka istraživanja "Siniša Stanković"",
journal = "Program i izvodi saopštenja : XVIII simpozijum Društva za fiziologiju biljaka Srbije, Vršac, 25-27. maj",
title = "In vitro regeneracija pupoljaka iz segmenata kotiledona, hipokotila i  korena kod Brassica oleraceae ssp., In vitro shoot regeneration from cotyledon, hypocotyl and root explants  in  Brassica oleraceae ssp.",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_rivec_578"
}

Pavlović, S., Adžić, S.,& Vinterhalter, B.. (2009). In vitro regeneracija pupoljaka iz segmenata kotiledona, hipokotila i  korena kod Brassica oleraceae ssp.. in Program i izvodi saopštenja : XVIII simpozijum Društva za fiziologiju biljaka Srbije, Vršac, 25-27. maj
Beograd : Društvo za fiziologiju biljaka Srbije., 47.
https://hdl.handle.net/21.15107/rcub_rivec_578

Pavlović S, Adžić S, Vinterhalter B. In vitro regeneracija pupoljaka iz segmenata kotiledona, hipokotila i  korena kod Brassica oleraceae ssp.. in Program i izvodi saopštenja : XVIII simpozijum Društva za fiziologiju biljaka Srbije, Vršac, 25-27. maj. 2009;:47.
https://hdl.handle.net/21.15107/rcub_rivec_578 .

Pavlović, Suzana, Adžić, Slađan, Vinterhalter, Branka, "In vitro regeneracija pupoljaka iz segmenata kotiledona, hipokotila i  korena kod Brassica oleraceae ssp." in Program i izvodi saopštenja : XVIII simpozijum Društva za fiziologiju biljaka Srbije, Vršac, 25-27. maj (2009):47,
https://hdl.handle.net/21.15107/rcub_rivec_578 .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana
AU  - Ninković, S.
AU  - Miljuš-Đukić, J
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
PY  - 2006
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/57
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata
EP  - 530
IS  - 4
SP  - 525
VL  - 50
DO  - 10.1007/s10535-006-0083-4
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana and Ninković, S. and Miljuš-Đukić, J and Vinterhalter, Branka and Vinterhalter, Dragan",
year = "2006",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata",
pages = "530-525",
number = "4",
volume = "50",
doi = "10.1007/s10535-006-0083-4"
}

Sretenović-Rajičić, T., Ninković, S., Miljuš-Đukić, J., Vinterhalter, B.,& Vinterhalter, D.. (2006). Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 50(4), 525-530.
https://doi.org/10.1007/s10535-006-0083-4

Sretenović-Rajičić T, Ninković S, Miljuš-Đukić J, Vinterhalter B, Vinterhalter D. Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum. 2006;50(4):525-530.
doi:10.1007/s10535-006-0083-4 .

Sretenović-Rajičić, Tatjana, Ninković, S., Miljuš-Đukić, J, Vinterhalter, Branka, Vinterhalter, Dragan, "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata" in Biologia Plantarum, 50, no. 4 (2006):525-530,
https://doi.org/10.1007/s10535-006-0083-4 . .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana D.
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
PY  - 2006
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/221
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.
T2  - Biologia Plantarum
T1  - Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata
EP  - 530
IS  - 4
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_rivec_221
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana D. and Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Vinterhalter, Branka and Vinterhalter, Dragan V",
year = "2006",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.",
journal = "Biologia Plantarum",
title = "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata",
pages = "530",
number = "4",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_rivec_221"
}

Sretenović-Rajičić, T. D., Ninković, S., Miljuš-Đukić, J. D., Vinterhalter, B.,& Vinterhalter, D. V.. (2006). Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_rivec_221

Sretenović-Rajičić TD, Ninković S, Miljuš-Đukić JD, Vinterhalter B, Vinterhalter DV. Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum. 2006;50(4):null-530.
https://hdl.handle.net/21.15107/rcub_rivec_221 .

Sretenović-Rajičić, Tatjana D., Ninković, Slavica, Miljuš-Đukić, Jovanka D., Vinterhalter, Branka, Vinterhalter, Dragan V, "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_rivec_221 .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana
AU  - Ninković, S.
AU  - Vinterhalter, Branka
AU  - Miljuš-Đukić, J
AU  - Vinterhalter, Dragan
PY  - 2004
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/55
AB  - Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Introduction of resistance to herbicide Basta (R) in Savoy cabbage
EP  - 436
IS  - 3
SP  - 431
VL  - 48
DO  - 10.1023/B:BIOP.0000041098.18836.be
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana and Ninković, S. and Vinterhalter, Branka and Miljuš-Đukić, J and Vinterhalter, Dragan",
year = "2004",
abstract = "Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Introduction of resistance to herbicide Basta (R) in Savoy cabbage",
pages = "436-431",
number = "3",
volume = "48",
doi = "10.1023/B:BIOP.0000041098.18836.be"
}

Sretenović-Rajičić, T., Ninković, S., Vinterhalter, B., Miljuš-Đukić, J.,& Vinterhalter, D.. (2004). Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 48(3), 431-436.
https://doi.org/10.1023/B:BIOP.0000041098.18836.be

Sretenović-Rajičić T, Ninković S, Vinterhalter B, Miljuš-Đukić J, Vinterhalter D. Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum. 2004;48(3):431-436.
doi:10.1023/B:BIOP.0000041098.18836.be .

Sretenović-Rajičić, Tatjana, Ninković, S., Vinterhalter, Branka, Miljuš-Đukić, J, Vinterhalter, Dragan, "Introduction of resistance to herbicide Basta (R) in Savoy cabbage" in Biologia Plantarum, 48, no. 3 (2004):431-436,
https://doi.org/10.1023/B:BIOP.0000041098.18836.be . .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana
AU  - Sušić, Zoran
AU  - Pavlović, Nenad
AU  - Ivančević, Mirjana
AU  - Vinterhalter, Branka
PY  - 2000
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/26
AB  - Kupusnjače (Brassicaceae) su ekonomski veoma značajna familija biljaka. U poslednjih trideset godina jako mnogo se radi na poboljšavanju karakteristika vrsta roda Brassica. Medu novijim metodama koje se koriste u oplemenjivačke svrhe na kupusima jesu i tehnike genetičkog inženjerstva. Cilj ovog rada bio je da razmotri transformacije kupusnjača, prikaže preliminarne rezultate dobijem u našoj laboratoriji, gde je izvršena inokulacija nekoliko genotipova roda Brassica. Korišćenje soj A. tumefaciens koji nosi bar gen za rezistenciju na herbicid Basta. kao i gen za rezistenciju na kanamicin (nptII) i reporter gen za-glukuronidazu (GUS). U radu je dat i osvrt na svrsishodnost transformacija Brassicacea, kao i na eventualne rizike koje korišćenje transgenih kupusa nosi.
AB  - Brassicaceae are economically very important crop family. Improvement of its characteristics is in focus for the last thirty years very much. One of the methods of biotechnology used in Brassica genus is genetic transfor­mation. In this paper we have discussed transformations of Brassica genus, comparing it with the results that we achieved in our laboratory. We have performed inoculation with Agrobacterium tumefacien, with the construct containing resistance to herbicide Basta (bar), marker genes for Kanamycin resistance (npt II) and reporter gene-glucuronidase. We have also discussed risks of transgenic crop use and seed development.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Genetičke transformacije familije Brassicaceae
T1  - Transformations of Brassicaceae family
EP  - 116
IS  - 1-2
SP  - 111
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rivec_26
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana and Sušić, Zoran and Pavlović, Nenad and Ivančević, Mirjana and Vinterhalter, Branka",
year = "2000",
abstract = "Kupusnjače (Brassicaceae) su ekonomski veoma značajna familija biljaka. U poslednjih trideset godina jako mnogo se radi na poboljšavanju karakteristika vrsta roda Brassica. Medu novijim metodama koje se koriste u oplemenjivačke svrhe na kupusima jesu i tehnike genetičkog inženjerstva. Cilj ovog rada bio je da razmotri transformacije kupusnjača, prikaže preliminarne rezultate dobijem u našoj laboratoriji, gde je izvršena inokulacija nekoliko genotipova roda Brassica. Korišćenje soj A. tumefaciens koji nosi bar gen za rezistenciju na herbicid Basta. kao i gen za rezistenciju na kanamicin (nptII) i reporter gen za-glukuronidazu (GUS). U radu je dat i osvrt na svrsishodnost transformacija Brassicacea, kao i na eventualne rizike koje korišćenje transgenih kupusa nosi., Brassicaceae are economically very important crop family. Improvement of its characteristics is in focus for the last thirty years very much. One of the methods of biotechnology used in Brassica genus is genetic transfor­mation. In this paper we have discussed transformations of Brassica genus, comparing it with the results that we achieved in our laboratory. We have performed inoculation with Agrobacterium tumefacien, with the construct containing resistance to herbicide Basta (bar), marker genes for Kanamycin resistance (npt II) and reporter gene-glucuronidase. We have also discussed risks of transgenic crop use and seed development.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Genetičke transformacije familije Brassicaceae, Transformations of Brassicaceae family",
pages = "116-111",
number = "1-2",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rivec_26"
}

Sretenović-Rajičić, T., Sušić, Z., Pavlović, N., Ivančević, M.,& Vinterhalter, B.. (2000). Genetičke transformacije familije Brassicaceae. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 7(1-2), 111-116.
https://hdl.handle.net/21.15107/rcub_rivec_26

Sretenović-Rajičić T, Sušić Z, Pavlović N, Ivančević M, Vinterhalter B. Genetičke transformacije familije Brassicaceae. in Selekcija i semenarstvo. 2000;7(1-2):111-116.
https://hdl.handle.net/21.15107/rcub_rivec_26 .

Sretenović-Rajičić, Tatjana, Sušić, Zoran, Pavlović, Nenad, Ivančević, Mirjana, Vinterhalter, Branka, "Genetičke transformacije familije Brassicaceae" in Selekcija i semenarstvo, 7, no. 1-2 (2000):111-116,
https://hdl.handle.net/21.15107/rcub_rivec_26 .

TY  - CONF
AU  - Sretenović-Rajičić, Tatjana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 1997
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/21
AB  - Considering the great toxicity paraquat has to a wide range of plant and animal species, including man, we have investigated the possible use of cabbage in vitro cultures as a model system suitable to study this pesticide. The main aim of this study was to investigate the effect of paraquat on growth and development of cabbage shoot cultures. Special attention was paid to the detection of eventual means by which cultures can overcome the detrimental effect of the chemical, and also to investigate if some of the genotypes coming from the breeding program have an increased tolerance to paraquat. Promising domestic genotypes PI94, P295, P6p, P44 were investigated in the rooting stage. A root induction medium containing MS inorganic salts and 5.0 mgl-1 IB A was also supplemented with Paraquat at 0.5, 2.0, 10.0, 50.0 mgl -1. Shoots were treated for 24 hours and then transferred to a hormone free medium. After 28 days we measured shoot length, number of internodes, percentage of rooting, root length and formation of laterals. Paraquat decreased all the parameters which were followed. On leaves it induced lesions. Genotypes P44 and P6p were more tolerant to paraquat then the other two genotypes. Genotype P44 was interesting since in some plants lateral shoot formation occurred.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro
EP  - 593
SP  - 589
VL  - 462
DO  - 10.17660/actahortic.1997.462.87
ER  - 

@conference{
author = "Sretenović-Rajičić, Tatjana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "1997",
abstract = "Considering the great toxicity paraquat has to a wide range of plant and animal species, including man, we have investigated the possible use of cabbage in vitro cultures as a model system suitable to study this pesticide. The main aim of this study was to investigate the effect of paraquat on growth and development of cabbage shoot cultures. Special attention was paid to the detection of eventual means by which cultures can overcome the detrimental effect of the chemical, and also to investigate if some of the genotypes coming from the breeding program have an increased tolerance to paraquat. Promising domestic genotypes PI94, P295, P6p, P44 were investigated in the rooting stage. A root induction medium containing MS inorganic salts and 5.0 mgl-1 IB A was also supplemented with Paraquat at 0.5, 2.0, 10.0, 50.0 mgl -1. Shoots were treated for 24 hours and then transferred to a hormone free medium. After 28 days we measured shoot length, number of internodes, percentage of rooting, root length and formation of laterals. Paraquat decreased all the parameters which were followed. On leaves it induced lesions. Genotypes P44 and P6p were more tolerant to paraquat then the other two genotypes. Genotype P44 was interesting since in some plants lateral shoot formation occurred.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro",
pages = "593-589",
volume = "462",
doi = "10.17660/actahortic.1997.462.87"
}

Sretenović-Rajičić, T., Vinterhalter, D.,& Vinterhalter, B.. (1997). The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro. in Acta Horticulturae
International Society for Horticultural Science., 462, 589-593.
https://doi.org/10.17660/actahortic.1997.462.87

Sretenović-Rajičić T, Vinterhalter D, Vinterhalter B. The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro. in Acta Horticulturae. 1997;462:589-593.
doi:10.17660/actahortic.1997.462.87 .

Sretenović-Rajičić, Tatjana, Vinterhalter, Dragan, Vinterhalter, Branka, "The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro" in Acta Horticulturae, 462 (1997):589-593,
https://doi.org/10.17660/actahortic.1997.462.87 . .