TY  - CONF
AU  - Đorđević, Nikola
AU  - Dervišević, Marina
AU  - Knežević, Isidora
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Đorđević, Snežana
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/607
AB  - Botrytis cinerea, prouzrokovač sive truleži, široko je rasprostranjen i štetan patogen na
velikom broju biljaka, uključujući vinovu lozu i voće, kao i povrće, naročito u stakleničkoj i
plasteničkoj proizvodnji. Za suzbijanje ovog patogena primenjuju se različiti fungicidi
hemijske prirode, a u poslednje vreme, primenu nalaze i brojni biokontrolni agensi,
uključujući bakterije roda Bacillus, zbog čega je cilj ovog rada bio da se ispita antifungalna
aktivnost bakterijskih sojeva u suzbijanju Botrytis cinerea.
U ovom istraživanju, korišćeno je 12 bakterijskih sojeva roda Bacillus (Bacillus
velezensis 31/18 RK 1-1, Bacillus amyloliquefaciens 31/18 RK 1-2, Bacillus licheniformis 31/18
RS-9, Bacillus mojavensis 31/18 5b2, Bacillus aerius 92/17 7, Bacillus marisflavi 6E, Bacillus
pumilus W27-2, Bacillus macroides W29-5, Bacillus megaterium S1, Bacillus thuringiensis BCE,
Bacillus amyloliquefaciens B002, Bacillus proteolyticus 7E). Antifungalna aktivnost ispitivanih
sojeva roda Bacillus prema Botrytis cinerea, testirana je u laboratorijskim uslovima, metodom
68
dvojne kultivacije, a nakon 7 dana inkubacije očitavani su rezultati. Svi ispitivani sojevi
bakterija, testirani su i na plodovima krastavca, u različitim koncentracijama primene (1%,
0,5% i 0,1%).
Rezultati ispitivanja antifungalne aktivnosti bakterijskih sojeva roda Bacillus prema B.
cinerea pokazali su da je najveći procenat inhibicije od 57,7% zabeležen kod Bacillus
amyloliquefaciens 31/18 RK 1-2, zatim 50,7% kod Bacillus velezensis 31/18 RK 1-1 i 45,4%
kod Bacillus mojavensis 31/18 5b2, dok su ostali sojevi pokazali manju inhibitornu aktivnost.
U bitestu na plodovima krastavca najbolji rezultati su zabeleženi u tretmanu sa Bacillus
licheniformis 31/18 RS-9 gde je rast micelije B. cinerea zaustavljen u tretmanima sa svakom od
primenjenih koncentracija. Takođe, dobro antagonističko delovanje pokazalo se i kod Bacillus
mojavensis 31/18 5b2 pri primenjenim koncentracijama 1 i 0.5%. Iz tog razloga, bakterije
roda Bacillus imaju dobar potencijal za upotrebu u suzbijanju fitopatogene gljive B. cinerea.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar
T1  - Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea
EP  - 68
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_rivec_607
ER  - 

@conference{
author = "Đorđević, Nikola and Dervišević, Marina and Knežević, Isidora and Trkulja, Nenad and Milosavljević, Anja and Đorđević, Snežana",
year = "2021",
abstract = "Botrytis cinerea, prouzrokovač sive truleži, široko je rasprostranjen i štetan patogen na
velikom broju biljaka, uključujući vinovu lozu i voće, kao i povrće, naročito u stakleničkoj i
plasteničkoj proizvodnji. Za suzbijanje ovog patogena primenjuju se različiti fungicidi
hemijske prirode, a u poslednje vreme, primenu nalaze i brojni biokontrolni agensi,
uključujući bakterije roda Bacillus, zbog čega je cilj ovog rada bio da se ispita antifungalna
aktivnost bakterijskih sojeva u suzbijanju Botrytis cinerea.
U ovom istraživanju, korišćeno je 12 bakterijskih sojeva roda Bacillus (Bacillus
velezensis 31/18 RK 1-1, Bacillus amyloliquefaciens 31/18 RK 1-2, Bacillus licheniformis 31/18
RS-9, Bacillus mojavensis 31/18 5b2, Bacillus aerius 92/17 7, Bacillus marisflavi 6E, Bacillus
pumilus W27-2, Bacillus macroides W29-5, Bacillus megaterium S1, Bacillus thuringiensis BCE,
Bacillus amyloliquefaciens B002, Bacillus proteolyticus 7E). Antifungalna aktivnost ispitivanih
sojeva roda Bacillus prema Botrytis cinerea, testirana je u laboratorijskim uslovima, metodom
68
dvojne kultivacije, a nakon 7 dana inkubacije očitavani su rezultati. Svi ispitivani sojevi
bakterija, testirani su i na plodovima krastavca, u različitim koncentracijama primene (1%,
0,5% i 0,1%).
Rezultati ispitivanja antifungalne aktivnosti bakterijskih sojeva roda Bacillus prema B.
cinerea pokazali su da je najveći procenat inhibicije od 57,7% zabeležen kod Bacillus
amyloliquefaciens 31/18 RK 1-2, zatim 50,7% kod Bacillus velezensis 31/18 RK 1-1 i 45,4%
kod Bacillus mojavensis 31/18 5b2, dok su ostali sojevi pokazali manju inhibitornu aktivnost.
U bitestu na plodovima krastavca najbolji rezultati su zabeleženi u tretmanu sa Bacillus
licheniformis 31/18 RS-9 gde je rast micelije B. cinerea zaustavljen u tretmanima sa svakom od
primenjenih koncentracija. Takođe, dobro antagonističko delovanje pokazalo se i kod Bacillus
mojavensis 31/18 5b2 pri primenjenim koncentracijama 1 i 0.5%. Iz tog razloga, bakterije
roda Bacillus imaju dobar potencijal za upotrebu u suzbijanju fitopatogene gljive B. cinerea.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar",
title = "Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea",
pages = "68-67",
url = "https://hdl.handle.net/21.15107/rcub_rivec_607"
}

Đorđević, N., Dervišević, M., Knežević, I., Trkulja, N., Milosavljević, A.,& Đorđević, S.. (2021). Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar
Beograd : Društvo za zaštitu bilja Srbije., 67-68.
https://hdl.handle.net/21.15107/rcub_rivec_607

Đorđević N, Dervišević M, Knežević I, Trkulja N, Milosavljević A, Đorđević S. Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar. 2021;:67-68.
https://hdl.handle.net/21.15107/rcub_rivec_607 .

Đorđević, Nikola, Dervišević, Marina, Knežević, Isidora, Trkulja, Nenad, Milosavljević, Anja, Đorđević, Snežana, "Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea" in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar (2021):67-68,
https://hdl.handle.net/21.15107/rcub_rivec_607 .

TY  - CONF
AU  - Dervišević, Marina
AU  - Đorđević, Nikola
AU  - Knežević, Isidora
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Đorđević, Snežana
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/606
AB  - Sclerotinia sclerotiorum (Lib.), prouzrokovač bele truleži, široko je rasprostranjena i
prouzrokuje štete na više od 400 biljaka domaćina, uključujući brojne ratarske i povrtarske
biljke, kako u polju, tako i u skladišnim uslovima. Kako plodored i hemijske metode, često nisu
dovoljno efikasni u kontroli ovog patogena, cilj ovog rada bio je da se ispita efikasnost
bakterijskih izolata roda Bacillus u suzbijanju S. sclerotiorum.
Antagonistička aktivnost 6 autohtonih bakterijskih izolata (Bacillus subtilis Z3, Bacillus
mojavensis 5B2, Bacillus mojavensis R6, Bacillus firmus S5, Bacillus proteolyticus 7E i Bacillus
thuringiensis 109 /18 1.) ispitivana je metodom dvojne kultivacije. U Petri kutijama sa
krompir-dekstroznim agarom (PDA) zasejani su fragmenti (Ø8 mm) izolata S. sclerotiorum, a
potom su bakteriološkom ezom, na udaljenosti 3 cm od fragmenata gljiva, naneti bakterijski
sojevi. U kontrolnom tretmanu, naneti su fragmenti micelije izolata fitopatogene gljjive bez
prisustva bakterijskog soja. Ogled je izveden u tri ponavljanja po tretmanu. Očitavanje
35
rezultata je urađeno nakon inkubacije u trajanju od 7 dana na temperaturi od 25 ± 1oC,
merenjem prečnika inhibicije. Bakterijski sojevi koji su pokazali najveću efikasnost u ovom
testu, ispitivani su i u biotestovima na listovima kupusa i krtolastom povrću (mrkva i
paštrnak) u laboratorijskim uslovima.
Bakterijski izolati Bacillus mojavensis 5B2, Bacillus mojavensis R-6 i Bacillus
thuringiensis 109/18 1. pokazali su antifungalnu aktivnost prema Sclerotinia sclerotiorum.
Najveći procenat inhibicije (PIRG) zabeležen je kod Bacillus mojavensis R6 (56.47%), zatim
kod B. mojavensis 5B2 (49.41%) i B. thuringiensis 109/18 1. (48.24%), dok Bacillus subtilis Z3,
Bacillus firmus S5 i Bacillus proteolyticus 7E nisu pokazali antifungalnu aktivnost prema S.
sclerociorum.
U biotestovima, bakterijski sojevi Bacillus mojavensis R6, B. mojavensis 5B2 i B.
thuringiensis 109/18 1. inhibirali su porast micelije i sklerocija i na taj način sprečili razvoj S.
sclerotiorum, za razliku od kontrolnih tretmana u kojima je došlo do formiranja
karakterističnih simptoma koje ovaj patogen prouzrokuje. Iz tog razloga, ovi bakterijski sojevi
mogu biti potencijalni biološki agensi za kontrolu S. sclerotiorum, na ekološki prihvatljiv i
siguran način.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar
T1  - Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum
EP  - 35
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_rivec_606
ER  - 

@conference{
author = "Dervišević, Marina and Đorđević, Nikola and Knežević, Isidora and Trkulja, Nenad and Milosavljević, Anja and Đorđević, Snežana",
year = "2021",
abstract = "Sclerotinia sclerotiorum (Lib.), prouzrokovač bele truleži, široko je rasprostranjena i
prouzrokuje štete na više od 400 biljaka domaćina, uključujući brojne ratarske i povrtarske
biljke, kako u polju, tako i u skladišnim uslovima. Kako plodored i hemijske metode, često nisu
dovoljno efikasni u kontroli ovog patogena, cilj ovog rada bio je da se ispita efikasnost
bakterijskih izolata roda Bacillus u suzbijanju S. sclerotiorum.
Antagonistička aktivnost 6 autohtonih bakterijskih izolata (Bacillus subtilis Z3, Bacillus
mojavensis 5B2, Bacillus mojavensis R6, Bacillus firmus S5, Bacillus proteolyticus 7E i Bacillus
thuringiensis 109 /18 1.) ispitivana je metodom dvojne kultivacije. U Petri kutijama sa
krompir-dekstroznim agarom (PDA) zasejani su fragmenti (Ø8 mm) izolata S. sclerotiorum, a
potom su bakteriološkom ezom, na udaljenosti 3 cm od fragmenata gljiva, naneti bakterijski
sojevi. U kontrolnom tretmanu, naneti su fragmenti micelije izolata fitopatogene gljjive bez
prisustva bakterijskog soja. Ogled je izveden u tri ponavljanja po tretmanu. Očitavanje
35
rezultata je urađeno nakon inkubacije u trajanju od 7 dana na temperaturi od 25 ± 1oC,
merenjem prečnika inhibicije. Bakterijski sojevi koji su pokazali najveću efikasnost u ovom
testu, ispitivani su i u biotestovima na listovima kupusa i krtolastom povrću (mrkva i
paštrnak) u laboratorijskim uslovima.
Bakterijski izolati Bacillus mojavensis 5B2, Bacillus mojavensis R-6 i Bacillus
thuringiensis 109/18 1. pokazali su antifungalnu aktivnost prema Sclerotinia sclerotiorum.
Najveći procenat inhibicije (PIRG) zabeležen je kod Bacillus mojavensis R6 (56.47%), zatim
kod B. mojavensis 5B2 (49.41%) i B. thuringiensis 109/18 1. (48.24%), dok Bacillus subtilis Z3,
Bacillus firmus S5 i Bacillus proteolyticus 7E nisu pokazali antifungalnu aktivnost prema S.
sclerociorum.
U biotestovima, bakterijski sojevi Bacillus mojavensis R6, B. mojavensis 5B2 i B.
thuringiensis 109/18 1. inhibirali su porast micelije i sklerocija i na taj način sprečili razvoj S.
sclerotiorum, za razliku od kontrolnih tretmana u kojima je došlo do formiranja
karakterističnih simptoma koje ovaj patogen prouzrokuje. Iz tog razloga, ovi bakterijski sojevi
mogu biti potencijalni biološki agensi za kontrolu S. sclerotiorum, na ekološki prihvatljiv i
siguran način.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar",
title = "Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum",
pages = "35-34",
url = "https://hdl.handle.net/21.15107/rcub_rivec_606"
}

Dervišević, M., Đorđević, N., Knežević, I., Trkulja, N., Milosavljević, A.,& Đorđević, S.. (2021). Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar
Beograd : Društvo za zaštitu bilja Srbije., 34-35.
https://hdl.handle.net/21.15107/rcub_rivec_606

Dervišević M, Đorđević N, Knežević I, Trkulja N, Milosavljević A, Đorđević S. Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar. 2021;:34-35.
https://hdl.handle.net/21.15107/rcub_rivec_606 .

Dervišević, Marina, Đorđević, Nikola, Knežević, Isidora, Trkulja, Nenad, Milosavljević, Anja, Đorđević, Snežana, "Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum" in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar (2021):34-35,
https://hdl.handle.net/21.15107/rcub_rivec_606 .

TY  - CONF
AU  - Knežević, Isidora
AU  - Dervišević, Marina
AU  - Đorđević, Nikola
AU  - Đorđević, Snežana
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/590
AB  - Fusarium graminearum Schwabe is one of the most important pathogens of wheat. The presence of this pathogen leads to loss of yield. The most common measure to control F. graminearum is the use of chemical compounds. Considering the constant application of compounds of the same mechanism of action, resistance to these compounds often occurs. The use of antagonistic bacteria has good potential in controlling this pathogen. In this research, the antifungal activity of antagonistic bacteria of the genus Bacillus on an isolate of F. graminearum under laboratory conditions was investigated. The inhibitory effect of 10 strains of bacteria was examined in the method of dual cultivation. Mycelia were transferred to a fresh PDA medium, and a suspension of bacteria was applied at 3 cm from the mycelial disc. After seven days of incubation at 25 ̊C, the inhibitory activity of specific bacterial strains against F. graminearum was observed. The highest percentage of inhibition was recorded in four strains of Bacillus mojavensis Roberts et al. (99/18 R-6, 31/18 RS 7-1, 31/18 RS-4 and 31/18 RS-8), in two strains of Bacillus velezensis Ruiz-García et al. (31/18 RK 1-1 and RK 3-2) and one strain of Bacillus amyloliquefaciens (Priest et al.) Borriss et al. 31/18 RK 1-2. The percentage of inhibition in these strains was between 30 and 40%. In two strains of Bacillus licheniformis (Weigmann) Chester 31/18 RS-9 and 31/18 RS-10, a percentage of inhibition of 11% and 17%, respectively, was recorded. In contrast, Bacillus licheniformis RS 92/17 6B did not show any inhibitory activity, according to F. graminearum. As this research indicates a good potential of Bacillus species in controlling F. graminearum, further research should examine their effectiveness in field conditions because the use of these bacteria would provide wheat protection without the possible risk of resistance.
PB  - Banja Luka : Poljoprivredni fakultet
C3  - Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina
T1  - Antifungal activity of Bacillus sp. against Fusarium graminearum
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_rivec_590
ER  - 

@conference{
author = "Knežević, Isidora and Dervišević, Marina and Đorđević, Nikola and Đorđević, Snežana",
year = "2021",
abstract = "Fusarium graminearum Schwabe is one of the most important pathogens of wheat. The presence of this pathogen leads to loss of yield. The most common measure to control F. graminearum is the use of chemical compounds. Considering the constant application of compounds of the same mechanism of action, resistance to these compounds often occurs. The use of antagonistic bacteria has good potential in controlling this pathogen. In this research, the antifungal activity of antagonistic bacteria of the genus Bacillus on an isolate of F. graminearum under laboratory conditions was investigated. The inhibitory effect of 10 strains of bacteria was examined in the method of dual cultivation. Mycelia were transferred to a fresh PDA medium, and a suspension of bacteria was applied at 3 cm from the mycelial disc. After seven days of incubation at 25 ̊C, the inhibitory activity of specific bacterial strains against F. graminearum was observed. The highest percentage of inhibition was recorded in four strains of Bacillus mojavensis Roberts et al. (99/18 R-6, 31/18 RS 7-1, 31/18 RS-4 and 31/18 RS-8), in two strains of Bacillus velezensis Ruiz-García et al. (31/18 RK 1-1 and RK 3-2) and one strain of Bacillus amyloliquefaciens (Priest et al.) Borriss et al. 31/18 RK 1-2. The percentage of inhibition in these strains was between 30 and 40%. In two strains of Bacillus licheniformis (Weigmann) Chester 31/18 RS-9 and 31/18 RS-10, a percentage of inhibition of 11% and 17%, respectively, was recorded. In contrast, Bacillus licheniformis RS 92/17 6B did not show any inhibitory activity, according to F. graminearum. As this research indicates a good potential of Bacillus species in controlling F. graminearum, further research should examine their effectiveness in field conditions because the use of these bacteria would provide wheat protection without the possible risk of resistance.",
publisher = "Banja Luka : Poljoprivredni fakultet",
journal = "Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina",
title = "Antifungal activity of Bacillus sp. against Fusarium graminearum",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_rivec_590"
}

Knežević, I., Dervišević, M., Đorđević, N.,& Đorđević, S.. (2021). Antifungal activity of Bacillus sp. against Fusarium graminearum. in Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina
Banja Luka : Poljoprivredni fakultet., 48.
https://hdl.handle.net/21.15107/rcub_rivec_590

Knežević I, Dervišević M, Đorđević N, Đorđević S. Antifungal activity of Bacillus sp. against Fusarium graminearum. in Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina. 2021;:48.
https://hdl.handle.net/21.15107/rcub_rivec_590 .

Knežević, Isidora, Dervišević, Marina, Đorđević, Nikola, Đorđević, Snežana, "Antifungal activity of Bacillus sp. against Fusarium graminearum" in Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina (2021):48,
https://hdl.handle.net/21.15107/rcub_rivec_590 .

TY  - CONF
AU  - Dervišević, Marina
AU  - Đorđević, Nikola
AU  - Knežević, Isidora
AU  - Đorđević, Snežana
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/589
AB  - Cercospora beticola Sacc. is the most economically significant sugar beet disease. In years suitable for developing the disease, the yield of the root and the total sugar content can be reduced by up to 50%. The application of chemical fungicides is a standard measure in the control of C. beticola. Still, the emergence of resistance have led to a decrease in available commercial agents'effectiveness. This study aimed to examine the efficacy of antagonistic bacterial isolates to control this pathogen under laboratory conditions. The antagonistic activity of four autochthonous bacterial isolates (Bacillus amyloliquefaciens (Priest et al.) Borriss et al. B002, Bacillus subtilis (Ehrenberg) Cohn Z3, Lactobacillus plantarum (Orla-Jensen) Bergey et al. L2 and Pediococcus pentosaceus Mees L5B) against three isolates of C. beticola (K1-2, K1-3, T2L2) was examined by dual cultivation method. In a bioassay, sugar beet leaves first were sprayed with a suspension of bacteria (2.8 x 1010 CFU/ml) after 24 hours of suspension of spores C. beticola. In the control, the leaves were sprayed only with the suspension of spores C. beticola. In each variant, there were six sugar beet leaves. After seven days, results were assessed by the Horsfall and Barratt (1945) scale. In the dual cultivation method, the highest percentage of inhibition (21.9 to 24%) was observed in the treatment with B. subtilis Z3 against all three isolates of C. beticola; B. amyloliquefaciens B002 and L. plantarum L2 showed a slightly lower percentage of inhibition, while P. pentosaceus L5B did not show any antagonistic activity against C. beticola isolates. In bioassay, isolates of B. subtilis Z3, B. amyloliquefaciens B002 and L. plantarum L2 had excellent preventive action against C. beticola. Their efficiency ranged from 87.48 to 99.17% compared to the control. These results indicate that the tested bacterial strains have good potential to control C. beticola, which can be an excellent alternative to chemical fungicides.
PB  - Banja Luka : Poljoprivredni fakultet
C3  - Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina
T1  - Antagonistic activity of bacterial isolates against Cercospora beticola in laboratory conditions
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rivec_589
ER  - 

@conference{
author = "Dervišević, Marina and Đorđević, Nikola and Knežević, Isidora and Đorđević, Snežana",
year = "2021",
abstract = "Cercospora beticola Sacc. is the most economically significant sugar beet disease. In years suitable for developing the disease, the yield of the root and the total sugar content can be reduced by up to 50%. The application of chemical fungicides is a standard measure in the control of C. beticola. Still, the emergence of resistance have led to a decrease in available commercial agents'effectiveness. This study aimed to examine the efficacy of antagonistic bacterial isolates to control this pathogen under laboratory conditions. The antagonistic activity of four autochthonous bacterial isolates (Bacillus amyloliquefaciens (Priest et al.) Borriss et al. B002, Bacillus subtilis (Ehrenberg) Cohn Z3, Lactobacillus plantarum (Orla-Jensen) Bergey et al. L2 and Pediococcus pentosaceus Mees L5B) against three isolates of C. beticola (K1-2, K1-3, T2L2) was examined by dual cultivation method. In a bioassay, sugar beet leaves first were sprayed with a suspension of bacteria (2.8 x 1010 CFU/ml) after 24 hours of suspension of spores C. beticola. In the control, the leaves were sprayed only with the suspension of spores C. beticola. In each variant, there were six sugar beet leaves. After seven days, results were assessed by the Horsfall and Barratt (1945) scale. In the dual cultivation method, the highest percentage of inhibition (21.9 to 24%) was observed in the treatment with B. subtilis Z3 against all three isolates of C. beticola; B. amyloliquefaciens B002 and L. plantarum L2 showed a slightly lower percentage of inhibition, while P. pentosaceus L5B did not show any antagonistic activity against C. beticola isolates. In bioassay, isolates of B. subtilis Z3, B. amyloliquefaciens B002 and L. plantarum L2 had excellent preventive action against C. beticola. Their efficiency ranged from 87.48 to 99.17% compared to the control. These results indicate that the tested bacterial strains have good potential to control C. beticola, which can be an excellent alternative to chemical fungicides.",
publisher = "Banja Luka : Poljoprivredni fakultet",
journal = "Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina",
title = "Antagonistic activity of bacterial isolates against Cercospora beticola in laboratory conditions",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_rivec_589"
}

Dervišević, M., Đorđević, N., Knežević, I.,& Đorđević, S.. (2021). Antagonistic activity of bacterial isolates against Cercospora beticola in laboratory conditions. in Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina
Banja Luka : Poljoprivredni fakultet., 47.
https://hdl.handle.net/21.15107/rcub_rivec_589

Dervišević M, Đorđević N, Knežević I, Đorđević S. Antagonistic activity of bacterial isolates against Cercospora beticola in laboratory conditions. in Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina. 2021;:47.
https://hdl.handle.net/21.15107/rcub_rivec_589 .

Dervišević, Marina, Đorđević, Nikola, Knežević, Isidora, Đorđević, Snežana, "Antagonistic activity of bacterial isolates against Cercospora beticola in laboratory conditions" in Book of Abstracts : X International Symposium on Agricultural Sciences "AgroReS 2021", 27-29, May, 2021 Trebinje, Bosnia and Herzegovina (2021):47,
https://hdl.handle.net/21.15107/rcub_rivec_589 .