TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/465
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
C3  - Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
EP  - 213
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_rivec_465
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213-213",
url = "https://hdl.handle.net/21.15107/rcub_rivec_465"
}

Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
East Sarajevo : Faculty of Agriculture., 213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465

Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10. 2021;:213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465 .

Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 (2021):213-213,
https://hdl.handle.net/21.15107/rcub_rivec_465 .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Calić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/214
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80-100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20-60% of regenerating explants. The explants from the majority of seedlings (52-100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10-20 (43.5%) up to>20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1-10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research, Berlin
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Calić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80-100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20-60% of regenerating explants. The explants from the majority of seedlings (52-100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10-20 (43.5%) up to>20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1-10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research, Berlin",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9"
}

Belić, M., Zdravković-Korać, S., Uzelac, B., Calić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research, Berlin., 10(1).
https://doi.org/10.1038/s41598-020-76279-9

Belić M, Zdravković-Korać S, Uzelac B, Calić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1).
doi:10.1038/s41598-020-76279-9 .

Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Calić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020),
https://doi.org/10.1038/s41598-020-76279-9 . .