TY  - JOUR
AU  - Jošić, Dragana
AU  - Cvikić, Dejan
AU  - Zdravković, Jasmina
AU  - Kuzmanović, Đorđe
AU  - Zdravković, Milan
AU  - Knežević, Magdalena
AU  - Pavlović, Snežana
PY  - 2017
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/194
AB  - The inhibitory effect of indigenous Pseudomonas strains on Fusarium spp. isolated from seeds of a new variety of carrot - 'Vizija' and their effects on seed germination were observed. Pseudomonas spp. strains were applied as bacterial suspensions (culture, 106 CFU mL-1) and cell-free supernatant (CFSa -106 and CFSb -108 CFU mL-1) fractions by sowing seeds during 7 (I) and 14 (II) days of incubation. The germination of control 'Vizija' seeds was 19% after the first and 40% after the second incubation period. Pseudomonas chlororaphis Q16 strain exhibited statistically significant increases in seed germination with all applied fractions and incubation periods, showing values of 43-62% and 55.5-91%, i.e. increments of 24-43% and 15.5-51% compared to the control, after 7 and 14 days of incubation, respectively. P. chlororaphis K35 showed lower but significantly different seed germination values (38-67%) for all variants, except for 7 days old culture. Pseudomonas sp. Ek1 had weaker seed germination potential, showing statistically significant increment only for CFSa,b (I) and CFSb (II) fractions. All tested Pseudomonas strains inhibited the growth of three Fusarium species isolated from 'Vizija' seeds: F. solani, F. oxysporum and F. subglutinans. Natural infection was observed in 20% (I) and 54% (II) of 'Vizija' seeds. P. chlororaphis K35 was the most efficient antifungal strain, reducing seed infection 97.5-100%, followed by Q16 with 95-100%, showing no statistically significant mutual difference. Pseudomonas sp. Ek1 showed a weaker antifungal activity and reduced seed infection by 85-96.75%. The application of P. chlororaphis Q16 and K35 as strains effective in improvement of carrot seed germination and growth inhibition of the seed pathogens F. solani, F. oxysporum and F. subglutinans, can be further tested in carrot production for more beneficial effects.
AB  - Ispitivan je inhibitorni efekat autohtonih Pseudomonas sojeva na gljive Fusarium spp. izolovane sa semena šargarepe sorte 'Vizija' i njihov uticaj na klijavost semena. Pseudomonas spp. sojevi su primenjeni u vidu bakterijske suspenzije (kulture, 106 CFU mL-1) i supernatanta (CFSa -106 i CFSb -108 CFU mL-1) potapanjem semena, a potom inkubirani tokom 7 (I) i 14 (II) dana. Klijavost semena 'Vizije' u kontroli iznosila je 19% posle prvog i 40% posle drugog perioda inkubacije. P. chlororaphis Q16 soj je uzrokovao statistički značajno povećanje klijavosti semena pri primeni svih frakcija i tokom oba inkubaciona perioda, dostižući 43-62% i 55.5-91% klijavosti, što je povećanje od 24-43% i 15.5-51% u poređenju sa kontrolom, posle 7 i 14 dana inkubacije. P. chlororaphis K35 je uslovio manje, ali statistički značajne vrednosti klijavosti semena (38-67%) za sve varijante, osim za kulturu posle 7 dana. Pseudomonas sp. Ek1 je pokazao slabiji potencijal klijavosti, sa statistički značajnim povećanjem samo za CFSa,b (I) i CFSb (II) frakcije. Svi ispitani Pseudomonas sojevi su inhibirali tri vrste gljiva iz roda Fusarium koje su izolovane sa semena 'Vizije': F. solani, F. oxysporum i F. subglutinans. Prirodna infekcija semena je uočena kod 20% (I) i 54% (II) semena 'Vizije' u kontrolnoj varijanti. P. chlororaphis K35 je ispoljio najače antifungalno dejstvo, sa smanjenjem infekcije semena 97.5-100%, a zatim soj Q16 sa 95-100%, bez statističke značajnosti među njima. Pseudomonas sp. Ek1 je ispoljio slabiju antifungalnu aktivnost i redukovao infekciju semena 85-96.75%. Primena P. chlororaphis Q16 i K35, kao sojeva koji efikasno povećavaju klijavost semena šargarepe i inhibiraju rast patogena semena F. solani, F. oxysporum i F. subglutinans, biće dalje testirana u proizvodnji šargarepe kako bi se procenili ostali pozitivni efekti ovih sojeva.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.
T1  - Daucus carrota L. - klijavost semena i prirodna infekcija semena Fusarium spp. pod uticajem Pseudomonas spp.
EP  - 195
IS  - 3-4
SP  - 189
VL  - 32
DO  - 10.2298/PIF1704189J
ER  - 

@article{
author = "Jošić, Dragana and Cvikić, Dejan and Zdravković, Jasmina and Kuzmanović, Đorđe and Zdravković, Milan and Knežević, Magdalena and Pavlović, Snežana",
year = "2017",
abstract = "The inhibitory effect of indigenous Pseudomonas strains on Fusarium spp. isolated from seeds of a new variety of carrot - 'Vizija' and their effects on seed germination were observed. Pseudomonas spp. strains were applied as bacterial suspensions (culture, 106 CFU mL-1) and cell-free supernatant (CFSa -106 and CFSb -108 CFU mL-1) fractions by sowing seeds during 7 (I) and 14 (II) days of incubation. The germination of control 'Vizija' seeds was 19% after the first and 40% after the second incubation period. Pseudomonas chlororaphis Q16 strain exhibited statistically significant increases in seed germination with all applied fractions and incubation periods, showing values of 43-62% and 55.5-91%, i.e. increments of 24-43% and 15.5-51% compared to the control, after 7 and 14 days of incubation, respectively. P. chlororaphis K35 showed lower but significantly different seed germination values (38-67%) for all variants, except for 7 days old culture. Pseudomonas sp. Ek1 had weaker seed germination potential, showing statistically significant increment only for CFSa,b (I) and CFSb (II) fractions. All tested Pseudomonas strains inhibited the growth of three Fusarium species isolated from 'Vizija' seeds: F. solani, F. oxysporum and F. subglutinans. Natural infection was observed in 20% (I) and 54% (II) of 'Vizija' seeds. P. chlororaphis K35 was the most efficient antifungal strain, reducing seed infection 97.5-100%, followed by Q16 with 95-100%, showing no statistically significant mutual difference. Pseudomonas sp. Ek1 showed a weaker antifungal activity and reduced seed infection by 85-96.75%. The application of P. chlororaphis Q16 and K35 as strains effective in improvement of carrot seed germination and growth inhibition of the seed pathogens F. solani, F. oxysporum and F. subglutinans, can be further tested in carrot production for more beneficial effects., Ispitivan je inhibitorni efekat autohtonih Pseudomonas sojeva na gljive Fusarium spp. izolovane sa semena šargarepe sorte 'Vizija' i njihov uticaj na klijavost semena. Pseudomonas spp. sojevi su primenjeni u vidu bakterijske suspenzije (kulture, 106 CFU mL-1) i supernatanta (CFSa -106 i CFSb -108 CFU mL-1) potapanjem semena, a potom inkubirani tokom 7 (I) i 14 (II) dana. Klijavost semena 'Vizije' u kontroli iznosila je 19% posle prvog i 40% posle drugog perioda inkubacije. P. chlororaphis Q16 soj je uzrokovao statistički značajno povećanje klijavosti semena pri primeni svih frakcija i tokom oba inkubaciona perioda, dostižući 43-62% i 55.5-91% klijavosti, što je povećanje od 24-43% i 15.5-51% u poređenju sa kontrolom, posle 7 i 14 dana inkubacije. P. chlororaphis K35 je uslovio manje, ali statistički značajne vrednosti klijavosti semena (38-67%) za sve varijante, osim za kulturu posle 7 dana. Pseudomonas sp. Ek1 je pokazao slabiji potencijal klijavosti, sa statistički značajnim povećanjem samo za CFSa,b (I) i CFSb (II) frakcije. Svi ispitani Pseudomonas sojevi su inhibirali tri vrste gljiva iz roda Fusarium koje su izolovane sa semena 'Vizije': F. solani, F. oxysporum i F. subglutinans. Prirodna infekcija semena je uočena kod 20% (I) i 54% (II) semena 'Vizije' u kontrolnoj varijanti. P. chlororaphis K35 je ispoljio najače antifungalno dejstvo, sa smanjenjem infekcije semena 97.5-100%, a zatim soj Q16 sa 95-100%, bez statističke značajnosti među njima. Pseudomonas sp. Ek1 je ispoljio slabiju antifungalnu aktivnost i redukovao infekciju semena 85-96.75%. Primena P. chlororaphis Q16 i K35, kao sojeva koji efikasno povećavaju klijavost semena šargarepe i inhibiraju rast patogena semena F. solani, F. oxysporum i F. subglutinans, biće dalje testirana u proizvodnji šargarepe kako bi se procenili ostali pozitivni efekti ovih sojeva.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp., Daucus carrota L. - klijavost semena i prirodna infekcija semena Fusarium spp. pod uticajem Pseudomonas spp.",
pages = "195-189",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704189J"
}

Jošić, D., Cvikić, D., Zdravković, J., Kuzmanović, Đ., Zdravković, M., Knežević, M.,& Pavlović, S.. (2017). Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 32(3-4), 189-195.
https://doi.org/10.2298/PIF1704189J

Jošić D, Cvikić D, Zdravković J, Kuzmanović Đ, Zdravković M, Knežević M, Pavlović S. Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.. in Pesticidi i fitomedicina. 2017;32(3-4):189-195.
doi:10.2298/PIF1704189J .

Jošić, Dragana, Cvikić, Dejan, Zdravković, Jasmina, Kuzmanović, Đorđe, Zdravković, Milan, Knežević, Magdalena, Pavlović, Snežana, "Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp." in Pesticidi i fitomedicina, 32, no. 3-4 (2017):189-195,
https://doi.org/10.2298/PIF1704189J . .

TY  - JOUR
AU  - Ugrinović, Milan
AU  - Mijatović, Mirjana
AU  - Zdravković, Jasmina
AU  - Girek, Zdenka
AU  - Kuzmanović, Đorđe
AU  - Rasulić, Nataša
AU  - Jošić, Dragana
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/160
AB  - The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants' yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)(5)-PCR fingerprinting showed presence of six different patterns on the 92% similarity level.
PB  - Scientific Agricultural Soc Finland, Univ Helsinski
T2  - Agricultural and Food Science
T1  - Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia
EP  - 185
IS  - 3
SP  - 173
VL  - 23
DO  - 10.23986/afsci.41385
ER  - 

@article{
author = "Ugrinović, Milan and Mijatović, Mirjana and Zdravković, Jasmina and Girek, Zdenka and Kuzmanović, Đorđe and Rasulić, Nataša and Jošić, Dragana",
year = "2014",
abstract = "The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants' yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)(5)-PCR fingerprinting showed presence of six different patterns on the 92% similarity level.",
publisher = "Scientific Agricultural Soc Finland, Univ Helsinski",
journal = "Agricultural and Food Science",
title = "Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia",
pages = "185-173",
number = "3",
volume = "23",
doi = "10.23986/afsci.41385"
}

Ugrinović, M., Mijatović, M., Zdravković, J., Girek, Z., Kuzmanović, Đ., Rasulić, N.,& Jošić, D.. (2014). Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia. in Agricultural and Food Science
Scientific Agricultural Soc Finland, Univ Helsinski., 23(3), 173-185.
https://doi.org/10.23986/afsci.41385

Ugrinović M, Mijatović M, Zdravković J, Girek Z, Kuzmanović Đ, Rasulić N, Jošić D. Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia. in Agricultural and Food Science. 2014;23(3):173-185.
doi:10.23986/afsci.41385 .

Ugrinović, Milan, Mijatović, Mirjana, Zdravković, Jasmina, Girek, Zdenka, Kuzmanović, Đorđe, Rasulić, Nataša, Jošić, Dragana, "Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia" in Agricultural and Food Science, 23, no. 3 (2014):173-185,
https://doi.org/10.23986/afsci.41385 . .