TY  - JOUR
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Stojanović, S.
AU  - Popović, Tatjana
AU  - Dolovac, Nenad
AU  - Zdravković, Jasmina
AU  - Pavlović, Snežana
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/146
AB  - Saponaria officinalis L. (Caryophyllaceae; also known as bouncingbet or soapwort) is a perennial medicinal plant important for the pharmaceutical industry and used as an expectorant, alterative, laxative, and ointment for some skin diseases and arthritic conditions. S. officinalis plants with typical symptoms (23% in 2011 and 47% in 2012) of phytoplasma infection were observed in Pancevo plantation, Serbia. The symptoms appeared in May with leaves changing color from green to brown with severe reddening and necrosis. Severely diseased plants died. The infected plants had a significant reduction in biomass and quality. To investigate the presence of phytoplasma, total DNA was extracted from 10 symptomatic and four asymptomatic plants by a CTAB method. The nested PCR was carried out using phytoplasma-specific primer set P1/16S-SR followed by R16F2n/R16R2, targeting the 16S rRNA gene sequence of 1.5 and 1.2 kb in length, respectively. The amplicons of expected size were obtained from the symptomatic plants, but not from the asymptomatic plants. To obtain restriction fragment length polymorphism (RFLP) patterns, the R16F2n/R2 amplicons were digested with AluI, TruI1, HpaII, and HhaI endonucleases. The resulting patterns indicated that seven plants were infected by a Stolbur phytoplasma belonging to the 16SrXII-A subgroup, since it had the identical RFLP pattern as the STOL reference strain. The 1.2 kb nested PCR products of representative isolate Sap7 were purified using PCR purification kit (Fermentas, Vilnius, Lithuania) according to the recommended protocol and sequenced using facilities of IMGGI SeqService, Belgrade, Serbia. The obtained sequence was deposited in the NCBI database (GenBank Accession No. JX866951). The phytoplasma 16S rRNA gene sequence from Sap7 had a sequence identity of 97% with GenBank accessions GQ273961.1 (‘Euonymus japonicus’ phytoplasma), JX311953.1 (Candidatus Phytoplasma solani clone 5043), JQ412100.1 (Iranian alfalfa phytoplasma M21), and JN561702.1 (‘Convolvulus arvensis’ stolbur phytoplasma clone P1/P7-Conv2/2010-Bg). To our knowledge, this is the first report of a natural infection of S. officinalis by 16SrXII-A subgroup (Stolbur) phytoplasma in Serbia. As cited by Lee et al. (1), the 16SrI-M subgroup phytoplasma in S. officinalis sample was already detected in Lithuania by Valiunas (2). The identification of phytoplasma in the Pancevo plantation caused the intensification of our biological control tests and efforts to reduce the ecological and economic impacts of these phytoplasmas.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia
EP  - 420
EP  - Saponaria officinalis L.
EP  - Caryophyllaceae
EP  - bouncingbet
EP  - soapwort
IS  - 3
SP  - 420
VL  - 97
DO  - 10.1094/PDIS-09-12-0885-PDN
ER  - 

@article{
author = "Jošić, Dragana and Starović, Mira and Stojanović, S. and Popović, Tatjana and Dolovac, Nenad and Zdravković, Jasmina and Pavlović, Snežana",
year = "2013",
abstract = "Saponaria officinalis L. (Caryophyllaceae; also known as bouncingbet or soapwort) is a perennial medicinal plant important for the pharmaceutical industry and used as an expectorant, alterative, laxative, and ointment for some skin diseases and arthritic conditions. S. officinalis plants with typical symptoms (23% in 2011 and 47% in 2012) of phytoplasma infection were observed in Pancevo plantation, Serbia. The symptoms appeared in May with leaves changing color from green to brown with severe reddening and necrosis. Severely diseased plants died. The infected plants had a significant reduction in biomass and quality. To investigate the presence of phytoplasma, total DNA was extracted from 10 symptomatic and four asymptomatic plants by a CTAB method. The nested PCR was carried out using phytoplasma-specific primer set P1/16S-SR followed by R16F2n/R16R2, targeting the 16S rRNA gene sequence of 1.5 and 1.2 kb in length, respectively. The amplicons of expected size were obtained from the symptomatic plants, but not from the asymptomatic plants. To obtain restriction fragment length polymorphism (RFLP) patterns, the R16F2n/R2 amplicons were digested with AluI, TruI1, HpaII, and HhaI endonucleases. The resulting patterns indicated that seven plants were infected by a Stolbur phytoplasma belonging to the 16SrXII-A subgroup, since it had the identical RFLP pattern as the STOL reference strain. The 1.2 kb nested PCR products of representative isolate Sap7 were purified using PCR purification kit (Fermentas, Vilnius, Lithuania) according to the recommended protocol and sequenced using facilities of IMGGI SeqService, Belgrade, Serbia. The obtained sequence was deposited in the NCBI database (GenBank Accession No. JX866951). The phytoplasma 16S rRNA gene sequence from Sap7 had a sequence identity of 97% with GenBank accessions GQ273961.1 (‘Euonymus japonicus’ phytoplasma), JX311953.1 (Candidatus Phytoplasma solani clone 5043), JQ412100.1 (Iranian alfalfa phytoplasma M21), and JN561702.1 (‘Convolvulus arvensis’ stolbur phytoplasma clone P1/P7-Conv2/2010-Bg). To our knowledge, this is the first report of a natural infection of S. officinalis by 16SrXII-A subgroup (Stolbur) phytoplasma in Serbia. As cited by Lee et al. (1), the 16SrI-M subgroup phytoplasma in S. officinalis sample was already detected in Lithuania by Valiunas (2). The identification of phytoplasma in the Pancevo plantation caused the intensification of our biological control tests and efforts to reduce the ecological and economic impacts of these phytoplasmas.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia",
pages = "420-Saponaria officinalis L.-Caryophyllaceae-bouncingbet-soapwort-420",
number = "3",
volume = "97",
doi = "10.1094/PDIS-09-12-0885-PDN"
}

Jošić, D., Starović, M., Stojanović, S., Popović, T., Dolovac, N., Zdravković, J.,& Pavlović, S.. (2013). First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(3), 420-420.
https://doi.org/10.1094/PDIS-09-12-0885-PDN

Jošić D, Starović M, Stojanović S, Popović T, Dolovac N, Zdravković J, Pavlović S. First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia. in Plant Disease. 2013;97(3):420-420.
doi:10.1094/PDIS-09-12-0885-PDN .

Jošić, Dragana, Starović, Mira, Stojanović, S., Popović, Tatjana, Dolovac, Nenad, Zdravković, Jasmina, Pavlović, Snežana, "First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia" in Plant Disease, 97, no. 3 (2013):420-420,
https://doi.org/10.1094/PDIS-09-12-0885-PDN . .

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Dolovac, Nenad
AU  - Ivanović, Marija
AU  - Damnjanović, Jelena
AU  - Zečević, Bogoljub
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/132
AB  - Volatile phase of essential oils of Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum and Origanum vulgare were tested for their effectiveness in control of Verticillium dahliae, in vitro. Percentage of inhibition of radial growth was calculated four days after exposure of mycelia to volatiles of essential oils. Also MIC and MFC were determined as well seven i.e. fourteen days after exposure. Highest value of inhibition expressed essential oil of O. vulgare with MIC and MFC applied at 0,04μl/ml of air, followed by essential oils of P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Essential oil of E. citiodora expressed MIC and MFC applied at 0,6μl/ml of air, while essential oil of M. piperita expressed only MIC when applied at 0,3μl/ml of air. Essential oils of P. sylvestris, R. officinalis and P. crispum din not express MIC or MFC even when applied at highest rate. .
AB  - Ispitivana je efikasnost isparljive faze eteričnih ulja Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum i Origanum vulgare u inhibiciji porasta micelije patogena Verticillium dahliae u in vitro uslovima. Procenat inhibicije radijalnog porasta izračunavan je četiri dana od izlaganja micelije dejstvu para eteričnih ulja.. Takođe, izračunavana je i minimalna inhibitorna koncentracija (MIC) sedam dana nakon izlaganja kao i minimalna fungicidna koncentracija (MFC) četrnaest dana od izlaganja dejstvu para. Najveći procenat inhibicije pokazalo je ulje O. vulgare sa MIC i MFC primenjenim već u najnižoj koncentraciji 0,04μl/ml vazduha, za kojim sledi ulje P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Eterično ulje E. citriodora ostvarilo je MIC i MFC primenjeno u koncentraciji 0,6μl/ml vazduha, dok je eterično ulje M. piperita pokazalo samo MIC i to primenjeno u konc. 0,3μl/ml vazduha. Eterična ulja P. sylvestris, R. officinalis i P. crispum nisu pokazala zadovoljavajući nivo inhibicije a samim tim nisu imala ni MIC ni MFC vrednost. .
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Effectiveness of essential oils in control of Verticillium dahliae in vitro
T1  - Efikasnost eteričnih ulja u suzbijanju Verticillium dahliae patogena paradajza in vitro
EP  - 168
IS  - 3
SP  - 162
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_rivec_132
ER  - 

@article{
author = "Đorđević, Mladen and Dolovac, Nenad and Ivanović, Marija and Damnjanović, Jelena and Zečević, Bogoljub",
year = "2013",
abstract = "Volatile phase of essential oils of Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum and Origanum vulgare were tested for their effectiveness in control of Verticillium dahliae, in vitro. Percentage of inhibition of radial growth was calculated four days after exposure of mycelia to volatiles of essential oils. Also MIC and MFC were determined as well seven i.e. fourteen days after exposure. Highest value of inhibition expressed essential oil of O. vulgare with MIC and MFC applied at 0,04μl/ml of air, followed by essential oils of P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Essential oil of E. citiodora expressed MIC and MFC applied at 0,6μl/ml of air, while essential oil of M. piperita expressed only MIC when applied at 0,3μl/ml of air. Essential oils of P. sylvestris, R. officinalis and P. crispum din not express MIC or MFC even when applied at highest rate. ., Ispitivana je efikasnost isparljive faze eteričnih ulja Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum i Origanum vulgare u inhibiciji porasta micelije patogena Verticillium dahliae u in vitro uslovima. Procenat inhibicije radijalnog porasta izračunavan je četiri dana od izlaganja micelije dejstvu para eteričnih ulja.. Takođe, izračunavana je i minimalna inhibitorna koncentracija (MIC) sedam dana nakon izlaganja kao i minimalna fungicidna koncentracija (MFC) četrnaest dana od izlaganja dejstvu para. Najveći procenat inhibicije pokazalo je ulje O. vulgare sa MIC i MFC primenjenim već u najnižoj koncentraciji 0,04μl/ml vazduha, za kojim sledi ulje P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Eterično ulje E. citriodora ostvarilo je MIC i MFC primenjeno u koncentraciji 0,6μl/ml vazduha, dok je eterično ulje M. piperita pokazalo samo MIC i to primenjeno u konc. 0,3μl/ml vazduha. Eterična ulja P. sylvestris, R. officinalis i P. crispum nisu pokazala zadovoljavajući nivo inhibicije a samim tim nisu imala ni MIC ni MFC vrednost. .",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Effectiveness of essential oils in control of Verticillium dahliae in vitro, Efikasnost eteričnih ulja u suzbijanju Verticillium dahliae patogena paradajza in vitro",
pages = "168-162",
number = "3",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_rivec_132"
}

Đorđević, M., Dolovac, N., Ivanović, M., Damnjanović, J.,& Zečević, B.. (2013). Effectiveness of essential oils in control of Verticillium dahliae in vitro. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 64(3), 162-168.
https://hdl.handle.net/21.15107/rcub_rivec_132

Đorđević M, Dolovac N, Ivanović M, Damnjanović J, Zečević B. Effectiveness of essential oils in control of Verticillium dahliae in vitro. in Zaštita bilja. 2013;64(3):162-168.
https://hdl.handle.net/21.15107/rcub_rivec_132 .

Đorđević, Mladen, Dolovac, Nenad, Ivanović, Marija, Damnjanović, Jelena, Zečević, Bogoljub, "Effectiveness of essential oils in control of Verticillium dahliae in vitro" in Zaštita bilja, 64, no. 3 (2013):162-168,
https://hdl.handle.net/21.15107/rcub_rivec_132 .

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Dolovac, Nenad
AU  - Đorđević, Radiša
AU  - Trkulja, Nenad
AU  - Damnjanović, Jelena
AU  - Zdravković, Jasmina
AU  - Mijatović, Mirjana
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/116
AB  - Aim of this study is to determine the impact of race 3 of fusarium wilt on some tomato cultivars if it occurs in Serbia. For this purpose eleven tomato cultivars were inoculated with this pathogen: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1 by applying classic method of inoculation by submersing the injured root in fungi suspension. Disease was assessed 30 days after inoculation using an ordinal scale range from 1 - 5. After assessment, Nada F1 and 129-Sprin had lowest average disease rating (2,9), and marked as tolerant. All the other cultivars were consider susceptible with ADR values higher than 3,0 in the following order: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Based on these results we can conclude that if the race 3 of Fusarium oxysporum f. sp. lycopersici occur in Serbia it could seriously jeopardize tomato production.
AB  - Cilj ovog istraživanja je da se utvrdi kakav bi bio uticaj rase 3 fuzarioznog uvenuća paradajza na pojedine kultivare ukoliko bi se ova rasa pojavila u Srbiji. U te svrhe inokulisano je jedanaest kultivara ovim patogenom i to: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1, primenom klasične metode inokulacije umakanjem povređenog korena u suspenziju gljiva. Nakon 30 dana rađena je procena pojave oboljenja upotrebom skale od 1 - 5. Nakon evaluacije, Nada F1 i 129-Sprin su imali najnižu vrednost ADR-a (prosečni nivo oboljenja) (2,9), i obeleženi su kao tolerantni. Svi ostali kultivari smatrani su osetljivim sa vrednostima ADR višim od 3,0, po sledećem rasporedu: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Na osnovu ovih rezultata možemo zaključiti da ako bi se rasa 3 Fusarium oxysporum f. sp. lycopersici pojavila u Srbiji mogla bi značajno da ugrozi proizvodnju paradajza.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars
T1  - Uticaj rase 3 Fusarium oxysporum f.sp. lycopersici na pojedine kultivare paradajza
EP  - 28
IS  - 1
SP  - 22
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_rivec_116
ER  - 

@article{
author = "Đorđević, Mladen and Dolovac, Nenad and Đorđević, Radiša and Trkulja, Nenad and Damnjanović, Jelena and Zdravković, Jasmina and Mijatović, Mirjana",
year = "2012",
abstract = "Aim of this study is to determine the impact of race 3 of fusarium wilt on some tomato cultivars if it occurs in Serbia. For this purpose eleven tomato cultivars were inoculated with this pathogen: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1 by applying classic method of inoculation by submersing the injured root in fungi suspension. Disease was assessed 30 days after inoculation using an ordinal scale range from 1 - 5. After assessment, Nada F1 and 129-Sprin had lowest average disease rating (2,9), and marked as tolerant. All the other cultivars were consider susceptible with ADR values higher than 3,0 in the following order: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Based on these results we can conclude that if the race 3 of Fusarium oxysporum f. sp. lycopersici occur in Serbia it could seriously jeopardize tomato production., Cilj ovog istraživanja je da se utvrdi kakav bi bio uticaj rase 3 fuzarioznog uvenuća paradajza na pojedine kultivare ukoliko bi se ova rasa pojavila u Srbiji. U te svrhe inokulisano je jedanaest kultivara ovim patogenom i to: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1, primenom klasične metode inokulacije umakanjem povređenog korena u suspenziju gljiva. Nakon 30 dana rađena je procena pojave oboljenja upotrebom skale od 1 - 5. Nakon evaluacije, Nada F1 i 129-Sprin su imali najnižu vrednost ADR-a (prosečni nivo oboljenja) (2,9), i obeleženi su kao tolerantni. Svi ostali kultivari smatrani su osetljivim sa vrednostima ADR višim od 3,0, po sledećem rasporedu: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Na osnovu ovih rezultata možemo zaključiti da ako bi se rasa 3 Fusarium oxysporum f. sp. lycopersici pojavila u Srbiji mogla bi značajno da ugrozi proizvodnju paradajza.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars, Uticaj rase 3 Fusarium oxysporum f.sp. lycopersici na pojedine kultivare paradajza",
pages = "28-22",
number = "1",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_rivec_116"
}

Đorđević, M., Dolovac, N., Đorđević, R., Trkulja, N., Damnjanović, J., Zdravković, J.,& Mijatović, M.. (2012). Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 63(1), 22-28.
https://hdl.handle.net/21.15107/rcub_rivec_116

Đorđević M, Dolovac N, Đorđević R, Trkulja N, Damnjanović J, Zdravković J, Mijatović M. Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars. in Zaštita bilja. 2012;63(1):22-28.
https://hdl.handle.net/21.15107/rcub_rivec_116 .

Đorđević, Mladen, Dolovac, Nenad, Đorđević, Radiša, Trkulja, Nenad, Damnjanović, Jelena, Zdravković, Jasmina, Mijatović, Mirjana, "Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars" in Zaštita bilja, 63, no. 1 (2012):22-28,
https://hdl.handle.net/21.15107/rcub_rivec_116 .