TY  - JOUR
AU  - Dinić, Zoran
AU  - Ugrinović, Milan
AU  - Bosnić, Predrag
AU  - Mijatović, Mirjana
AU  - Zdravković, Jasmina
AU  - Miladinović, Miroslav
AU  - Jošić, Dragana
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/152
AB  - Phosphorus (P) is one of the major essential macronutrient for plant growth. Phosphate solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study endophytic bacteria were isolated form nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4. Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml-1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis.
AB  - Fosfor (P) je jedan od esencijalnih makronutrijenata potrebnih za rast biljaka. Fosfosolubilizirajuće bakterije (PCB) spadaju u grupu heterotrofnih bakterija sposobnih da solubilizuju fosfor iz nerastvornih organskih i neorganskih jedinjenja, oslobađajući fosfor koji je dostupan biljkama. U ovom radu su ispitivane endofite bakterije izolovane iz nodula boranije gajene u različitim sistemima interkropinga. Samo 14 od 158 endofitih izolata je solubilizovalo trikalcijum fosfate (TCP). Nijedan izolat nije solubilizovao FePO4 i AlPO4. Osam izolata je formiralo zone solubilizacije šire od 3 mm i selektovano je za dalji rad. TCP solubilizacija je kvantifikovana u tečnom Pikovskaya medijumu i iznosila je 14-182 μgml-1 rastvorenog fosfora. TCP solubilizacija je praćena zakišeljavanjem medijuma, a izmereno je opadanje pH sa 7,4 na 5,57 i 4,91 kod izolata sa najmanjim i najvećim solubilizacionim potencijalom. Selektovan je najefikasniji izolat MP18 i identifikovan kao Pseudomonas sp. prema izvršenoj analizi sekvence 16S rRNK gena.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules
T1  - Solubilizacija neorganskih fosfata endofitim Pseudomonas sp. iz nodula boranije
EP  - 105
IS  - 2
SP  - 100
VL  - 51
DO  - 10.5937/ratpov51-6222
ER  - 

@article{
author = "Dinić, Zoran and Ugrinović, Milan and Bosnić, Predrag and Mijatović, Mirjana and Zdravković, Jasmina and Miladinović, Miroslav and Jošić, Dragana",
year = "2014",
abstract = "Phosphorus (P) is one of the major essential macronutrient for plant growth. Phosphate solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study endophytic bacteria were isolated form nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4. Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml-1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis., Fosfor (P) je jedan od esencijalnih makronutrijenata potrebnih za rast biljaka. Fosfosolubilizirajuće bakterije (PCB) spadaju u grupu heterotrofnih bakterija sposobnih da solubilizuju fosfor iz nerastvornih organskih i neorganskih jedinjenja, oslobađajući fosfor koji je dostupan biljkama. U ovom radu su ispitivane endofite bakterije izolovane iz nodula boranije gajene u različitim sistemima interkropinga. Samo 14 od 158 endofitih izolata je solubilizovalo trikalcijum fosfate (TCP). Nijedan izolat nije solubilizovao FePO4 i AlPO4. Osam izolata je formiralo zone solubilizacije šire od 3 mm i selektovano je za dalji rad. TCP solubilizacija je kvantifikovana u tečnom Pikovskaya medijumu i iznosila je 14-182 μgml-1 rastvorenog fosfora. TCP solubilizacija je praćena zakišeljavanjem medijuma, a izmereno je opadanje pH sa 7,4 na 5,57 i 4,91 kod izolata sa najmanjim i najvećim solubilizacionim potencijalom. Selektovan je najefikasniji izolat MP18 i identifikovan kao Pseudomonas sp. prema izvršenoj analizi sekvence 16S rRNK gena.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules, Solubilizacija neorganskih fosfata endofitim Pseudomonas sp. iz nodula boranije",
pages = "105-100",
number = "2",
volume = "51",
doi = "10.5937/ratpov51-6222"
}

Dinić, Z., Ugrinović, M., Bosnić, P., Mijatović, M., Zdravković, J., Miladinović, M.,& Jošić, D.. (2014). Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 51(2), 100-105.
https://doi.org/10.5937/ratpov51-6222

Dinić Z, Ugrinović M, Bosnić P, Mijatović M, Zdravković J, Miladinović M, Jošić D. Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo. 2014;51(2):100-105.
doi:10.5937/ratpov51-6222 .

Dinić, Zoran, Ugrinović, Milan, Bosnić, Predrag, Mijatović, Mirjana, Zdravković, Jasmina, Miladinović, Miroslav, Jošić, Dragana, "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules" in Ratarstvo i povrtarstvo, 51, no. 2 (2014):100-105,
https://doi.org/10.5937/ratpov51-6222 . .