Obradović, Aleksa

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orcid::0000-0002-9425-3871
  • Obradović, Aleksa (21)

Author's Bibliography

Изолација врста Streptomyces из пољопривредног и непољопривредног земљишта

Gavrilović, Bojana; Obradović, Aleksa

(Smederevska Palanka : Institut za povrtarstvo, 2024)

TY  - DATA
AU  - Gavrilović, Bojana
AU  - Obradović, Aleksa
PY  - 2024
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/892
AB  - На локалитету Смедеревска Паланка из узорака пољопривредног и непољопривредног земљишта изолаване су врсте рода Streptomyces. Укупно је изоловано 30 потенцијално различитих изолата. Највећи број изолата је изолован је из непољопривредног земљишта (21), док је далеко мањи број изолован из пољопривредног земљишта (отворено поље - 7 и заштићени простор - 2). Са петри шоља и из земљишта је извршена екстракција укупне ДНК. Након добијања чистих култура, планирано је изучавање биолошке активности изолата према неким фитопатогенима и њихов утицај на раст и развој биљака.  Појединачни изолати ће бити секвенцирани.
AB  - Streptomyces species were isolated from samples of agricultural and non-agricultural soil at the Smederevska Palanka site. A total of 30 potentially different isolates were isolated. The largest number of isolates was isolated from non-agricultural land (21), while a much smaller number was isolated from agricultural land (open field - 7 and protected area - 2). Total DNA was extracted from soil and Petri dishes. After obtaining pure cultures, it is planned to study the biological activity of the isolates against some phytopathogens and their influence on the growth and development of plants. Individual isolates will be sequenced.
PB  - Smederevska Palanka : Institut za povrtarstvo
T1  - Изолација врста Streptomyces из пољопривредног и непољопривредног земљишта
T1  - Isolation of Streptomyces species from agricultural and non-agricultural soil
UR  - https://hdl.handle.net/21.15107/rcub_rivec_892
ER  - 
@misc{
author = "Gavrilović, Bojana and Obradović, Aleksa",
year = "2024",
abstract = "На локалитету Смедеревска Паланка из узорака пољопривредног и непољопривредног земљишта изолаване су врсте рода Streptomyces. Укупно је изоловано 30 потенцијално различитих изолата. Највећи број изолата је изолован је из непољопривредног земљишта (21), док је далеко мањи број изолован из пољопривредног земљишта (отворено поље - 7 и заштићени простор - 2). Са петри шоља и из земљишта је извршена екстракција укупне ДНК. Након добијања чистих култура, планирано је изучавање биолошке активности изолата према неким фитопатогенима и њихов утицај на раст и развој биљака.  Појединачни изолати ће бити секвенцирани., Streptomyces species were isolated from samples of agricultural and non-agricultural soil at the Smederevska Palanka site. A total of 30 potentially different isolates were isolated. The largest number of isolates was isolated from non-agricultural land (21), while a much smaller number was isolated from agricultural land (open field - 7 and protected area - 2). Total DNA was extracted from soil and Petri dishes. After obtaining pure cultures, it is planned to study the biological activity of the isolates against some phytopathogens and their influence on the growth and development of plants. Individual isolates will be sequenced.",
publisher = "Smederevska Palanka : Institut za povrtarstvo",
title = "Изолација врста Streptomyces из пољопривредног и непољопривредног земљишта, Isolation of Streptomyces species from agricultural and non-agricultural soil",
url = "https://hdl.handle.net/21.15107/rcub_rivec_892"
}
Gavrilović, B.,& Obradović, A.. (2024). Изолација врста Streptomyces из пољопривредног и непољопривредног земљишта. 
Smederevska Palanka : Institut za povrtarstvo..
https://hdl.handle.net/21.15107/rcub_rivec_892
Gavrilović B, Obradović A. Изолација врста Streptomyces из пољопривредног и непољопривредног земљишта. 2024;.
https://hdl.handle.net/21.15107/rcub_rivec_892 .
Gavrilović, Bojana, Obradović, Aleksa, "Изолација врста Streptomyces из пољопривредног и непољопривредног земљишта" (2024),
https://hdl.handle.net/21.15107/rcub_rivec_892 .

Efikasnost bioloških preparata u kontroli bakteriozne pegavosti paprike

Gavrilović, Bojana; Ugrinović, Milan; Rakić, Ivan; Adamović, Jelena; Obradović, Aleksa

(Smederevska Palanka : Institut za povrtarstvo, 2023)

TY  - CONF
AU  - Gavrilović, Bojana
AU  - Ugrinović, Milan
AU  - Rakić, Ivan
AU  - Adamović, Jelena
AU  - Obradović, Aleksa
PY  - 2023
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/724
AB  - Bakteriozna pegavost lišća koju izaziva Xanthomonas euvesicatoria je
jedno od najznačajnih oboljenja paprike u Srbiji. U cilju zaštite paprike, korišćeni su biološki preparati Serenade ASO, Erwix, Bakterije, Ekstrasol i Bacillomix. Kao standard korišćen je preparat na bazi bakar-hidroksida (Everest). Ogled je postavljen u Institutu za povrtarstvo u Smederevskoj
Palanci tokom leta 2023. godine. Proučavanja su obavljena na sorti paprike Paraćinka u uslovima veštačke inokulacije u stakleniku. Najefikasniji je bio preparat na bazi bakar-hidroksida (96%), dok su biološki preparati ispoljili nižu efikasnost: Serenade ASO (71%), Bakterije (61%), Ekstrasol
(61%), Erwix (55%) i Bacillomix (54%). Ipak, svi proučavani tretmani su značajno smanjili intenzitet oboljenja u odnosu na kontrolu. Eksperiment
je izveden u stakleniku bez klimatizacije, pri visokim spoljnim
temperaturama, što je moglo nepovoljno uticati na efikasnost preparata
baziranih na biološkim agensima. Prilikom narednih ogleda neophodno je
obezbediti kontrolisane uslove. Treba isprobati i različito vreme primene i
integraciju sa drugim merama zaštite.
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most important pepper diseases in Serbia. In order to control this disease, we
tested efficacy of commercially available biological products: Serenade
ASO, Erwix, Bakterije, Ekstrasol and Bacillomix. A preparation based on
copper hydroxide (Everest) was used as a standard treatment. The
experiment was set up at the Institute for Vegetable Crops in Smederevska
Palanka during the summer of 2023. Experiment was performed on pepper
variety Paraćinka under artificial inoculation in a greenhouse. The most efficient was the standard treatment based on copper hydroxide (96%), while tested biocontrol treatments showed statistically lower efficacy:
Serenade ASO (71%), Bacteria (61%), Ekstrasol (61%), Erwix (55%) and Bacillomix (54%). However, all tested treatments significantly reduced intensity of the disease compared to the control. The experiment was performed in a nonclimatized greenhouse, which could adversely affect the effectiveness of biocontrol treatments. Therefore, subsequent trials, should
be carried out in controlled conditions. Different application times and
integration with other protection measures should also be tested.
PB  - Smederevska Palanka : Institut za povrtarstvo
C3  - Zbornik radova : Nacionalni naučni skup sa međunarodnim učešćem Biotehnologija i savremeni pristup u gajenju i oplemenjivanju bilja, Smederevska Palanka 2. novembar
T1  - Efikasnost bioloških preparata u kontroli bakteriozne pegavosti paprike
T1  - Efficacy of biological agents in control of pepper bacterial spot
EP  - 59
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_rivec_724
ER  - 
@conference{
author = "Gavrilović, Bojana and Ugrinović, Milan and Rakić, Ivan and Adamović, Jelena and Obradović, Aleksa",
year = "2023",
abstract = "Bakteriozna pegavost lišća koju izaziva Xanthomonas euvesicatoria je
jedno od najznačajnih oboljenja paprike u Srbiji. U cilju zaštite paprike, korišćeni su biološki preparati Serenade ASO, Erwix, Bakterije, Ekstrasol i Bacillomix. Kao standard korišćen je preparat na bazi bakar-hidroksida (Everest). Ogled je postavljen u Institutu za povrtarstvo u Smederevskoj
Palanci tokom leta 2023. godine. Proučavanja su obavljena na sorti paprike Paraćinka u uslovima veštačke inokulacije u stakleniku. Najefikasniji je bio preparat na bazi bakar-hidroksida (96%), dok su biološki preparati ispoljili nižu efikasnost: Serenade ASO (71%), Bakterije (61%), Ekstrasol
(61%), Erwix (55%) i Bacillomix (54%). Ipak, svi proučavani tretmani su značajno smanjili intenzitet oboljenja u odnosu na kontrolu. Eksperiment
je izveden u stakleniku bez klimatizacije, pri visokim spoljnim
temperaturama, što je moglo nepovoljno uticati na efikasnost preparata
baziranih na biološkim agensima. Prilikom narednih ogleda neophodno je
obezbediti kontrolisane uslove. Treba isprobati i različito vreme primene i
integraciju sa drugim merama zaštite., Bacterial spot caused by Xanthomonas euvesicatoria is one of the most important pepper diseases in Serbia. In order to control this disease, we
tested efficacy of commercially available biological products: Serenade
ASO, Erwix, Bakterije, Ekstrasol and Bacillomix. A preparation based on
copper hydroxide (Everest) was used as a standard treatment. The
experiment was set up at the Institute for Vegetable Crops in Smederevska
Palanka during the summer of 2023. Experiment was performed on pepper
variety Paraćinka under artificial inoculation in a greenhouse. The most efficient was the standard treatment based on copper hydroxide (96%), while tested biocontrol treatments showed statistically lower efficacy:
Serenade ASO (71%), Bacteria (61%), Ekstrasol (61%), Erwix (55%) and Bacillomix (54%). However, all tested treatments significantly reduced intensity of the disease compared to the control. The experiment was performed in a nonclimatized greenhouse, which could adversely affect the effectiveness of biocontrol treatments. Therefore, subsequent trials, should
be carried out in controlled conditions. Different application times and
integration with other protection measures should also be tested.",
publisher = "Smederevska Palanka : Institut za povrtarstvo",
journal = "Zbornik radova : Nacionalni naučni skup sa međunarodnim učešćem Biotehnologija i savremeni pristup u gajenju i oplemenjivanju bilja, Smederevska Palanka 2. novembar",
title = "Efikasnost bioloških preparata u kontroli bakteriozne pegavosti paprike, Efficacy of biological agents in control of pepper bacterial spot",
pages = "59-51",
url = "https://hdl.handle.net/21.15107/rcub_rivec_724"
}
Gavrilović, B., Ugrinović, M., Rakić, I., Adamović, J.,& Obradović, A.. (2023). Efikasnost bioloških preparata u kontroli bakteriozne pegavosti paprike. in Zbornik radova : Nacionalni naučni skup sa međunarodnim učešćem Biotehnologija i savremeni pristup u gajenju i oplemenjivanju bilja, Smederevska Palanka 2. novembar
Smederevska Palanka : Institut za povrtarstvo., 51-59.
https://hdl.handle.net/21.15107/rcub_rivec_724
Gavrilović B, Ugrinović M, Rakić I, Adamović J, Obradović A. Efikasnost bioloških preparata u kontroli bakteriozne pegavosti paprike. in Zbornik radova : Nacionalni naučni skup sa međunarodnim učešćem Biotehnologija i savremeni pristup u gajenju i oplemenjivanju bilja, Smederevska Palanka 2. novembar. 2023;:51-59.
https://hdl.handle.net/21.15107/rcub_rivec_724 .
Gavrilović, Bojana, Ugrinović, Milan, Rakić, Ivan, Adamović, Jelena, Obradović, Aleksa, "Efikasnost bioloških preparata u kontroli bakteriozne pegavosti paprike" in Zbornik radova : Nacionalni naučni skup sa međunarodnim učešćem Biotehnologija i savremeni pristup u gajenju i oplemenjivanju bilja, Smederevska Palanka 2. novembar (2023):51-59,
https://hdl.handle.net/21.15107/rcub_rivec_724 .

Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)

Gavrilović, Bojana; Ugrinović, Milan; Adamović, Jelena; Obradović, Aleksa

(Beograd: Društvo za zaštitu bilja Srbije, 2023)

TY  - CONF
AU  - Gavrilović, Bojana
AU  - Ugrinović, Milan
AU  - Adamović, Jelena
AU  - Obradović, Aleksa
PY  - 2023
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/806
AB  - U cilju proučavanja efikasnosti bioloških preparata u zaštiti paprika od prouzrokovača
bakteriozne pegavosti lišća (Xanthomonas euvesicatoria), tokom leta 2023. godine, postavljen
je ogled u stakleniku Instituta za povrtarstvo u Smederevskoj Palanci. Proučavana je
efikasnost komercijalno dostupnih bioloških preparate Serenade ASO, Erwix, Bakterije,
Ekstrasol i Bacillomix. Kao standard korišćen je preparat na bazi bakar-hidroksida (Everest).
Proučavanja su obavljena na sorti paprike Paraćinka. Preparati su primenjeni u sledećim
koncentracijama: Serenade ASO (4%), Erwix (2%), Bakterije (1,5%), Ekstrasol (1%),
Bacillomix (1%) i Everest (0,5%). Eksperimentalni dizajn ogleda je potpuno slučajan plan u tri
ponavljanja sa sledećim tretmanima: (a) biljke tretirane pojedinačnim preparatima i nakon
dva sata inokulisane suspenzijom bakterija; (b) biljke tretirane samo suspenzijom bakterija
(pozitivna kontrola); (v) biljke tretirane vodom (negativna kontrola). Inokulacija je vršena u
fenofazi 8-9 listova ručnom prskalicom sojem bakterije X. euvesicatoria KFB 1 (10ˉ8 cfu/mol).
Nakon tretmana, biljke su pokrivene plastičnim kesama, radi održavanja visoke vlažnosti u
trajanju 48 časova. Prvi simptomi su uočeni 10 dana nakon inokulacije, a efikasnost preparata
ocenjena je 3 nedelje posle inokulacije. Indeks oboljenja izračunat je preko površine
nekrotičnih pega na lišću, pomoću Horsfall-Barratt skale, a efikasnost proučavanih baktericida
izračunata je korišćenjem Abbott-ove jednačine. Za statističku obradu korišćena je metoda
analize varijanse, a za pojedinačna poređenja tretmana korišćeni su Dunnett-ov i Duncan-ov
test. Intenzitet zaraze u kontroli je iznosio 53%. Na osnovu dobijenih rezultata zaključuje se
da je najefikasniji bio preparat na bazi bakar-hidroksida (96%), dok su biološki preparati
ispoljili slabiji stepen efikasnosti: Serenade ASO (71%), Bakterije (61%), Ekstrasol (61%),
Erwix (55%), Bacillomix (54%). Među biološkim preparatima nije bilo statistički značajne
razlike dok je standardni tretman ispoljio značajno višu efikasnost. Treba naglasiti da je
eksperiment izveden u stakleniku bez klimatizacije pri visokim spoljnim temperaturama, što je moglo nepovoljno uticati na efikasnost preparata baziranih na biološkim agensima. Stoga je
u cilju poboljšanja efikasnosti ovih preparata, prilikom narednih ogleda, neophodno
obezbediti kontrolisane uslove, a takođe treba proučiti i različito vreme primene, kao i
integraciju sa drugim merama zaštite.
PB  - Beograd: Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor
T1  - Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)
EP  - 43
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_rivec_806
ER  - 
@conference{
author = "Gavrilović, Bojana and Ugrinović, Milan and Adamović, Jelena and Obradović, Aleksa",
year = "2023",
abstract = "U cilju proučavanja efikasnosti bioloških preparata u zaštiti paprika od prouzrokovača
bakteriozne pegavosti lišća (Xanthomonas euvesicatoria), tokom leta 2023. godine, postavljen
je ogled u stakleniku Instituta za povrtarstvo u Smederevskoj Palanci. Proučavana je
efikasnost komercijalno dostupnih bioloških preparate Serenade ASO, Erwix, Bakterije,
Ekstrasol i Bacillomix. Kao standard korišćen je preparat na bazi bakar-hidroksida (Everest).
Proučavanja su obavljena na sorti paprike Paraćinka. Preparati su primenjeni u sledećim
koncentracijama: Serenade ASO (4%), Erwix (2%), Bakterije (1,5%), Ekstrasol (1%),
Bacillomix (1%) i Everest (0,5%). Eksperimentalni dizajn ogleda je potpuno slučajan plan u tri
ponavljanja sa sledećim tretmanima: (a) biljke tretirane pojedinačnim preparatima i nakon
dva sata inokulisane suspenzijom bakterija; (b) biljke tretirane samo suspenzijom bakterija
(pozitivna kontrola); (v) biljke tretirane vodom (negativna kontrola). Inokulacija je vršena u
fenofazi 8-9 listova ručnom prskalicom sojem bakterije X. euvesicatoria KFB 1 (10ˉ8 cfu/mol).
Nakon tretmana, biljke su pokrivene plastičnim kesama, radi održavanja visoke vlažnosti u
trajanju 48 časova. Prvi simptomi su uočeni 10 dana nakon inokulacije, a efikasnost preparata
ocenjena je 3 nedelje posle inokulacije. Indeks oboljenja izračunat je preko površine
nekrotičnih pega na lišću, pomoću Horsfall-Barratt skale, a efikasnost proučavanih baktericida
izračunata je korišćenjem Abbott-ove jednačine. Za statističku obradu korišćena je metoda
analize varijanse, a za pojedinačna poređenja tretmana korišćeni su Dunnett-ov i Duncan-ov
test. Intenzitet zaraze u kontroli je iznosio 53%. Na osnovu dobijenih rezultata zaključuje se
da je najefikasniji bio preparat na bazi bakar-hidroksida (96%), dok su biološki preparati
ispoljili slabiji stepen efikasnosti: Serenade ASO (71%), Bakterije (61%), Ekstrasol (61%),
Erwix (55%), Bacillomix (54%). Među biološkim preparatima nije bilo statistički značajne
razlike dok je standardni tretman ispoljio značajno višu efikasnost. Treba naglasiti da je
eksperiment izveden u stakleniku bez klimatizacije pri visokim spoljnim temperaturama, što je moglo nepovoljno uticati na efikasnost preparata baziranih na biološkim agensima. Stoga je
u cilju poboljšanja efikasnosti ovih preparata, prilikom narednih ogleda, neophodno
obezbediti kontrolisane uslove, a takođe treba proučiti i različito vreme primene, kao i
integraciju sa drugim merama zaštite.",
publisher = "Beograd: Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor",
title = "Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)",
pages = "43-42",
url = "https://hdl.handle.net/21.15107/rcub_rivec_806"
}
Gavrilović, B., Ugrinović, M., Adamović, J.,& Obradović, A.. (2023). Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria). in Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor
Beograd: Društvo za zaštitu bilja Srbije., 42-43.
https://hdl.handle.net/21.15107/rcub_rivec_806
Gavrilović B, Ugrinović M, Adamović J, Obradović A. Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria). in Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor. 2023;:42-43.
https://hdl.handle.net/21.15107/rcub_rivec_806 .
Gavrilović, Bojana, Ugrinović, Milan, Adamović, Jelena, Obradović, Aleksa, "Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)" in Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor (2023):42-43,
https://hdl.handle.net/21.15107/rcub_rivec_806 .

Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)

Gavrilović, Bojana; Ugrinović, Milan; Adamović, Jelena; Obradović, Aleksa

(Beograd: Društvo za zaštitu bilja Srbije, 2023)

TY  - CONF
AU  - Gavrilović, Bojana
AU  - Ugrinović, Milan
AU  - Adamović, Jelena
AU  - Obradović, Aleksa
PY  - 2023
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/819
AB  - U cilju proučavanja efikasnosti bioloških preparata u zaštiti paprika od prouzrokovačabakteriozne pegavosti lišća (Xanthomonas euvesicatoria), tokom leta 2023. godine, postavljenje ogled u stakleniku Instituta za povrtarstvo u Smederevskoj Palanci. Proučavana jeefikasnost komercijalno dostupnih bioloških preparate Serenade ASO, Erwix, Bakterije,Ekstrasol i Bacillomix. Kao standard korišćen je preparat na bazi bakar-hidroksida (Everest).Proučavanja su obavljena na sorti paprike Paraćinka. Preparati su primenjeni u sledećimkoncentracijama: Serenade ASO (4%), Erwix (2%), Bakterije (1,5%), Ekstrasol (1%),Bacillomix (1%) i Everest (0,5%). Eksperimentalni dizajn ogleda je potpuno slučajan plan u triponavljanja sa sledećim tretmanima: (a) biljke tretirane pojedinačnim preparatima i nakondva sata inokulisane suspenzijom bakterija; (b) biljke tretirane samo suspenzijom bakterija(pozitivna kontrola); (v) biljke tretirane vodom (negativna kontrola). Inokulacija je vršena ufenofazi 8-9 listova ručnom prskalicom sojem bakterije X. euvesicatoria KFB 1 (10ˉ8 cfu/mol).Nakon tretmana, biljke su pokrivene plastičnim kesama, radi održavanja visoke vlažnosti utrajanju 48 časova. Prvi simptomi su uočeni 10 dana nakon inokulacije, a efikasnost preparataocenjena je 3 nedelje posle inokulacije. Indeks oboljenja izračunat je preko površinenekrotičnih pega na lišću, pomoću Horsfall-Barratt skale, a efikasnost proučavanih baktericidaizračunata je korišćenjem Abbott-ove jednačine. Za statističku obradu korišćena je metodaanalize varijanse, a za pojedinačna poređenja tretmana korišćeni su Dunnett-ov i Duncan-ovtest. Intenzitet zaraze u kontroli je iznosio 53%. Na osnovu dobijenih rezultata zaključuje seda je najefikasniji bio preparat na bazi bakar-hidroksida (96%), dok su biološki preparatiispoljili slabiji stepen efikasnosti: Serenade ASO (71%), Bakterije (61%), Ekstrasol (61%),Erwix (55%), Bacillomix (54%). Među biološkim preparatima nije bilo statistički značajnerazlike dok je standardni tretman ispoljio značajno višu efikasnost. Treba naglasiti da jeeksperiment izveden u stakleniku bez klimatizacije pri visokim spoljnim temperaturama, što je moglo nepovoljno uticati na efikasnost preparata baziranih na biološkim agensima. Stoga jeu cilju poboljšanja efikasnosti ovih preparata, prilikom narednih ogleda, neophodnoobezbediti kontrolisane uslove, a takođe treba proučiti i različito vreme primene, kao iintegraciju sa drugim merama zaštite.
PB  - Beograd: Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor
T1  - Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)
UR  - https://hdl.handle.net/21.15107/rcub_rivec_819
ER  - 
@conference{
author = "Gavrilović, Bojana and Ugrinović, Milan and Adamović, Jelena and Obradović, Aleksa",
year = "2023",
abstract = "U cilju proučavanja efikasnosti bioloških preparata u zaštiti paprika od prouzrokovačabakteriozne pegavosti lišća (Xanthomonas euvesicatoria), tokom leta 2023. godine, postavljenje ogled u stakleniku Instituta za povrtarstvo u Smederevskoj Palanci. Proučavana jeefikasnost komercijalno dostupnih bioloških preparate Serenade ASO, Erwix, Bakterije,Ekstrasol i Bacillomix. Kao standard korišćen je preparat na bazi bakar-hidroksida (Everest).Proučavanja su obavljena na sorti paprike Paraćinka. Preparati su primenjeni u sledećimkoncentracijama: Serenade ASO (4%), Erwix (2%), Bakterije (1,5%), Ekstrasol (1%),Bacillomix (1%) i Everest (0,5%). Eksperimentalni dizajn ogleda je potpuno slučajan plan u triponavljanja sa sledećim tretmanima: (a) biljke tretirane pojedinačnim preparatima i nakondva sata inokulisane suspenzijom bakterija; (b) biljke tretirane samo suspenzijom bakterija(pozitivna kontrola); (v) biljke tretirane vodom (negativna kontrola). Inokulacija je vršena ufenofazi 8-9 listova ručnom prskalicom sojem bakterije X. euvesicatoria KFB 1 (10ˉ8 cfu/mol).Nakon tretmana, biljke su pokrivene plastičnim kesama, radi održavanja visoke vlažnosti utrajanju 48 časova. Prvi simptomi su uočeni 10 dana nakon inokulacije, a efikasnost preparataocenjena je 3 nedelje posle inokulacije. Indeks oboljenja izračunat je preko površinenekrotičnih pega na lišću, pomoću Horsfall-Barratt skale, a efikasnost proučavanih baktericidaizračunata je korišćenjem Abbott-ove jednačine. Za statističku obradu korišćena je metodaanalize varijanse, a za pojedinačna poređenja tretmana korišćeni su Dunnett-ov i Duncan-ovtest. Intenzitet zaraze u kontroli je iznosio 53%. Na osnovu dobijenih rezultata zaključuje seda je najefikasniji bio preparat na bazi bakar-hidroksida (96%), dok su biološki preparatiispoljili slabiji stepen efikasnosti: Serenade ASO (71%), Bakterije (61%), Ekstrasol (61%),Erwix (55%), Bacillomix (54%). Među biološkim preparatima nije bilo statistički značajnerazlike dok je standardni tretman ispoljio značajno višu efikasnost. Treba naglasiti da jeeksperiment izveden u stakleniku bez klimatizacije pri visokim spoljnim temperaturama, što je moglo nepovoljno uticati na efikasnost preparata baziranih na biološkim agensima. Stoga jeu cilju poboljšanja efikasnosti ovih preparata, prilikom narednih ogleda, neophodnoobezbediti kontrolisane uslove, a takođe treba proučiti i različito vreme primene, kao iintegraciju sa drugim merama zaštite.",
publisher = "Beograd: Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor",
title = "Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)",
url = "https://hdl.handle.net/21.15107/rcub_rivec_819"
}
Gavrilović, B., Ugrinović, M., Adamović, J.,& Obradović, A.. (2023). Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria). in Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor
Beograd: Društvo za zaštitu bilja Srbije..
https://hdl.handle.net/21.15107/rcub_rivec_819
Gavrilović B, Ugrinović M, Adamović J, Obradović A. Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria). in Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor. 2023;.
https://hdl.handle.net/21.15107/rcub_rivec_819 .
Gavrilović, Bojana, Ugrinović, Milan, Adamović, Jelena, Obradović, Aleksa, "Efikasnost bioloških preparata u zaštiti paprike od prouzrokovača bakteriozne pegavosti lišća (Xanthomonas euvesicatoria)" in Zbornik rezimea radova/XVII simpozijuma o zaštiti bilja, 27.– 30. novembar 2023., Zlatibor (2023),
https://hdl.handle.net/21.15107/rcub_rivec_819 .

Integration of biological and conventional treatments in control of pepper bacterial spot

Šević, Milan; Gašić, Katarina; Ignjatov, Maja; Mijatović, Mirjana; Prokić, Anđelka; Obradović, Aleksa

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/203
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}
Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier Sci Ltd, Oxford., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006
Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .
Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .
20
5
15

Integration of biological and conventional treatments in control of pepper bacterial spot

Šević, Milan; Gašić, Katarina; Ignjatov, Maja; Mijatović, Mirjana; Prokić, Anđelka; Obradović, Aleksa

(Oxford : Elsevier Sci Ltd, 2019)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/220
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Oxford : Elsevier Sci Ltd
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Oxford : Elsevier Sci Ltd",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}
Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Oxford : Elsevier Sci Ltd., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006
Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .
Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .
20
5
15

Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot

Gašić, Katarina; Kuzmanović, Nemanja; Ivanović, Milan; Prokić, Anđelka; Šević, Milan; Obradović, Aleksa

(Frontiers Media Sa, Lausanne, 2018)

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/201
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 
@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Anđelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}
Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.02021
Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .
Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Anđelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .
5
40
18
36

Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology

Gašić, Katarina; Kuzmanović, Nemanja; Ivanović, Milan; Prokić, Anđelka; Šević, Milan; Obradović, Aleksa

(Frontiers Media Sa, Lausanne, 2018)

TY  - DATA
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/483
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
UR  - https://hdl.handle.net/21.15107/rcub_rivec_483
ER  - 
@misc{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Anđelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology",
url = "https://hdl.handle.net/21.15107/rcub_rivec_483"
}
Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne..
https://hdl.handle.net/21.15107/rcub_rivec_483
Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. in Frontiers in Microbiology. 2018;.
https://hdl.handle.net/21.15107/rcub_rivec_483 .
Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Anđelka, Šević, Milan, Obradović, Aleksa, "Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology" in Frontiers in Microbiology (2018),
https://hdl.handle.net/21.15107/rcub_rivec_483 .

Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot

Šević, Milan; Gašić, Katarina; Đorđević, Mladen; Ignjatov, Maja; Mijatović, Mirjana; Zečević, Bogoljub; Obradović, Aleksa

(International Society for Horticultural Science, 2016)

TY  - CONF
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Đorđević, Mladen
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Zečević, Bogoljub
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/185
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes
T1  - Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot
EP  - 150
SP  - 147
VL  - 1142
DO  - 10.17660/ActaHortic.2016.1142.23
ER  - 
@conference{
author = "Šević, Milan and Gašić, Katarina and Đorđević, Mladen and Ignjatov, Maja and Mijatović, Mirjana and Zečević, Bogoljub and Obradović, Aleksa",
year = "2016",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes",
title = "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot",
pages = "150-147",
volume = "1142",
doi = "10.17660/ActaHortic.2016.1142.23"
}
Šević, M., Gašić, K., Đorđević, M., Ignjatov, M., Mijatović, M., Zečević, B.,& Obradović, A.. (2016). Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes
International Society for Horticultural Science., 1142, 147-150.
https://doi.org/10.17660/ActaHortic.2016.1142.23
Šević M, Gašić K, Đorđević M, Ignjatov M, Mijatović M, Zečević B, Obradović A. Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes. 2016;1142:147-150.
doi:10.17660/ActaHortic.2016.1142.23 .
Šević, Milan, Gašić, Katarina, Đorđević, Mladen, Ignjatov, Maja, Mijatović, Mirjana, Zečević, Bogoljub, Obradović, Aleksa, "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot" in Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes, 1142 (2016):147-150,
https://doi.org/10.17660/ActaHortic.2016.1142.23 . .
3
1
2

Bacterial fruit blotch of watermelon in Serbia

Zlatković, Nevena; Prokić, Anđelka; Kuzmanović, Nemanja; Gašić, Katarina; Šević, Milan; Ivanović, Milan; Obradović, Aleksa

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2015)

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/171
AB  - During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014.
AB  - U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial fruit blotch of watermelon in Serbia
T1  - Bakteriozna mrljavost plodova lubenice u Srbiji
EP  - 271
IS  - 3
SP  - 265
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3880
ER  - 
@article{
author = "Zlatković, Nevena and Prokić, Anđelka and Kuzmanović, Nemanja and Gašić, Katarina and Šević, Milan and Ivanović, Milan and Obradović, Aleksa",
year = "2015",
abstract = "During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014., U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial fruit blotch of watermelon in Serbia, Bakteriozna mrljavost plodova lubenice u Srbiji",
pages = "271-265",
number = "3",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3880"
}
Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Šević, M., Ivanović, M.,& Obradović, A.. (2015). Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(3), 265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880
Zlatković N, Prokić A, Kuzmanović N, Gašić K, Šević M, Ivanović M, Obradović A. Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar. 2015;43(3):265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .
Zlatković, Nevena, Prokić, Anđelka, Kuzmanović, Nemanja, Gašić, Katarina, Šević, Milan, Ivanović, Milan, Obradović, Aleksa, "Bacterial fruit blotch of watermelon in Serbia" in Biljni lekar, 43, no. 3 (2015):265-271,
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential

Gašić, Katarina; Ivanović, Milan; Kuzmanović, Nemanja; Prokić, Anđelka; Obradović, Aleksa; Šević, Milan

(International Society for Horticultural Science, 2014)

TY  - CONF
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
AU  - Šević, Milan
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/154
AB  - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
EP  - 126
SP  - 123
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.17
ER  - 
@conference{
author = "Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Anđelka and Obradović, Aleksa and Šević, Milan",
year = "2014",
abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential",
pages = "126-123",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.17"
}
Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae
International Society for Horticultural Science., 1056, 123-126.
https://doi.org/10.17660/ActaHortic.2014.1056.17
Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae. 2014;1056:123-126.
doi:10.17660/ActaHortic.2014.1056.17 .
Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Anđelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in Acta Horticulturae, 1056 (2014):123-126,
https://doi.org/10.17660/ActaHortic.2014.1056.17 . .
2

New strategies for pepper and tomato bacterial spot control

Šević, Milan; Gašić, Katarina; Obradović, Aleksa

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2014)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/157
AB  - Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control.
AB  - Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - New strategies for pepper and tomato bacterial spot control
T1  - Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti
EP  - 307
IS  - 4
SP  - 296
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3603
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Obradović, Aleksa",
year = "2014",
abstract = "Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control., Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "New strategies for pepper and tomato bacterial spot control, Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti",
pages = "307-296",
number = "4",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3603"
}
Šević, M., Gašić, K.,& Obradović, A.. (2014). New strategies for pepper and tomato bacterial spot control. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 42(4), 296-307.
https://hdl.handle.net/21.15107/rcub_agrospace_3603
Šević M, Gašić K, Obradović A. New strategies for pepper and tomato bacterial spot control. in Biljni lekar. 2014;42(4):296-307.
https://hdl.handle.net/21.15107/rcub_agrospace_3603 .
Šević, Milan, Gašić, Katarina, Obradović, Aleksa, "New strategies for pepper and tomato bacterial spot control" in Biljni lekar, 42, no. 4 (2014):296-307,
https://hdl.handle.net/21.15107/rcub_agrospace_3603 .

Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts

Prokić, Anđelka; Gašić, Katarina; Ivanović, Milan; Kuzmanović, Nemanja; Šević, Milan; Pulawska, J.; Obradović, Aleksa

(Springer, New York, 2012)

TY  - JOUR
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Šević, Milan
AU  - Pulawska, J.
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/131
AB  - Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts
VL  - 94
DO  - 10.4454/jpp.v94i1sup.020
ER  - 
@article{
author = "Prokić, Anđelka and Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Šević, Milan and Pulawska, J. and Obradović, Aleksa",
year = "2012",
abstract = "Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts",
volume = "94",
doi = "10.4454/jpp.v94i1sup.020"
}
Prokić, A., Gašić, K., Ivanović, M., Kuzmanović, N., Šević, M., Pulawska, J.,& Obradović, A.. (2012). Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts. in Journal of Plant Pathology
Springer, New York., 94.
https://doi.org/10.4454/jpp.v94i1sup.020
Prokić A, Gašić K, Ivanović M, Kuzmanović N, Šević M, Pulawska J, Obradović A. Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts. in Journal of Plant Pathology. 2012;94.
doi:10.4454/jpp.v94i1sup.020 .
Prokić, Anđelka, Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Šević, Milan, Pulawska, J., Obradović, Aleksa, "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts" in Journal of Plant Pathology, 94 (2012),
https://doi.org/10.4454/jpp.v94i1sup.020 . .
1

Sensitivity of some pepper genotypes to bacterial spot causal agent

Ignjatov, Maja; Šević, Milan; Gašić, Katarina; Jovičić, Dušica; Nikolić, Zorica; Milošević, Dragana; Obradović, Aleksa

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2012)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/111
AB  - Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease.
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Sensitivity of some pepper genotypes to bacterial spot causal agent
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 
@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease., Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Sensitivity of some pepper genotypes to bacterial spot causal agent, Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}
Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769
Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .
Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Sensitivity of some pepper genotypes to bacterial spot causal agent" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .
4

Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia

Ignjatov, Maja; Gašić, Katarina; Ivanović, Milan; Šević, Milan; Obradović, Aleksa; Milošević, Mirjana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2010)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Šević, Milan
AU  - Obradović, Aleksa
AU  - Milošević, Mirjana
PY  - 2010
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/83
AB  - During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development.
AB  - Tokom 2008. godine prikupljeni su uzorci obolelog lišća paprike sa simptomima bakteriozne pegavosti iz različitih lokaliteta Republike Srbije. Izolacijom iz zaraženih listova dobijen je 101 soj bakterija. Cilj ovog rada je proučavanje odlika i identifikacija izolovanih sojeva bakterije u skladu sa najnovijom nomenklaturom. Patogene i biohemijsko-fiziološke karakteristike sojeva proučene su korišćenjem standardnih bakterioloških testova. Određivanje fizioloških rasa bakterijske populacije izvršeno je na osnovu reakcije diferencijalnih sorti paprike Early Calwonder (ECW), njenih izogenih linija (ECW-10, ECW-20 ECW-30) i reakcijom sorte Capsicum pubescens. Proučena je osetljivost sojeva u in vitro uslovima na streptomicin, kasugamicin i bakar-sulfat. Rezultati proučavanja biohemijsko-fizioloških odlika sojeva ukazuju da naši sojevi pripadaju genetičkoj grupi 'A', odnosno vrsti Xanthomonas euvesicatoria. Proučavani sojevi, takođe, predstavljaju heterogenu populaciju u kojoj su zastupljene četiri fiziološke rase bakterije X. euvesicatoria (P1, P3, P7, P8). Sojevi rezistentni na streptomicin nisu detektovani ovim istraživanjima. Otpornost na 50 ppm kasugamicina utvrđena je kod 6 sojeva, a 13 sojeva je bilo otporno na 200 ppm bakar-sulfata. Prikazani rezultati ukazuju na opasnost od razvoja rezistentnosti bakterija na ova jedinjenja.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia
T1  - Karakterizacija sojeva Xanthomonas euvesicatoria, patogena paprike u Srbiji
EP  - 149
IS  - 2
SP  - 139
VL  - 25
DO  - 10.2298/PIF1002139I
ER  - 
@article{
author = "Ignjatov, Maja and Gašić, Katarina and Ivanović, Milan and Šević, Milan and Obradović, Aleksa and Milošević, Mirjana",
year = "2010",
abstract = "During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development., Tokom 2008. godine prikupljeni su uzorci obolelog lišća paprike sa simptomima bakteriozne pegavosti iz različitih lokaliteta Republike Srbije. Izolacijom iz zaraženih listova dobijen je 101 soj bakterija. Cilj ovog rada je proučavanje odlika i identifikacija izolovanih sojeva bakterije u skladu sa najnovijom nomenklaturom. Patogene i biohemijsko-fiziološke karakteristike sojeva proučene su korišćenjem standardnih bakterioloških testova. Određivanje fizioloških rasa bakterijske populacije izvršeno je na osnovu reakcije diferencijalnih sorti paprike Early Calwonder (ECW), njenih izogenih linija (ECW-10, ECW-20 ECW-30) i reakcijom sorte Capsicum pubescens. Proučena je osetljivost sojeva u in vitro uslovima na streptomicin, kasugamicin i bakar-sulfat. Rezultati proučavanja biohemijsko-fizioloških odlika sojeva ukazuju da naši sojevi pripadaju genetičkoj grupi 'A', odnosno vrsti Xanthomonas euvesicatoria. Proučavani sojevi, takođe, predstavljaju heterogenu populaciju u kojoj su zastupljene četiri fiziološke rase bakterije X. euvesicatoria (P1, P3, P7, P8). Sojevi rezistentni na streptomicin nisu detektovani ovim istraživanjima. Otpornost na 50 ppm kasugamicina utvrđena je kod 6 sojeva, a 13 sojeva je bilo otporno na 200 ppm bakar-sulfata. Prikazani rezultati ukazuju na opasnost od razvoja rezistentnosti bakterija na ova jedinjenja.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia, Karakterizacija sojeva Xanthomonas euvesicatoria, patogena paprike u Srbiji",
pages = "149-139",
number = "2",
volume = "25",
doi = "10.2298/PIF1002139I"
}
Ignjatov, M., Gašić, K., Ivanović, M., Šević, M., Obradović, A.,& Milošević, M.. (2010). Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(2), 139-149.
https://doi.org/10.2298/PIF1002139I
Ignjatov M, Gašić K, Ivanović M, Šević M, Obradović A, Milošević M. Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticidi i fitomedicina. 2010;25(2):139-149.
doi:10.2298/PIF1002139I .
Ignjatov, Maja, Gašić, Katarina, Ivanović, Milan, Šević, Milan, Obradović, Aleksa, Milošević, Mirjana, "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia" in Pesticidi i fitomedicina, 25, no. 2 (2010):139-149,
https://doi.org/10.2298/PIF1002139I . .
10

Potato Virus Y (PVY) on pepper in Serbia

Mijatović, Mirjana; Ivanović, Miroslav; Obradović, Aleksa; Zečević, Bogoljub

(International Society for Horticultural Science, 2002)

TY  - CONF
AU  - Mijatović, Mirjana
AU  - Ivanović, Miroslav
AU  - Obradović, Aleksa
AU  - Zečević, Bogoljub
PY  - 2002
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/46
AB  - During 1997, samples of plants showing symptoms of a viral disease were collected from thirteen pepper growing areas. On some plants characteristic symptoms of Potato Virus Y (PVY) infection were observed. Pepper samples were tested by applying the direct immunoenzyme test (DAS ELISA). For the identification of the virus a PVY antiserum was used, i.e. the kit of Loewe Biochemica, GmbH Germany. Isolation of the virus was performed by the method of mechanical inoculation using 0.01 M phosphate buffer (pH-7) and 0.1% Na2SO3 from the infected pepper samples. Identification of the virus strains was performed on the basis of the reaction of differential pepper genotypes. The virus was identified according to pepper disease symptoms, DAS ELISA test, reaction of inoculated test plants, way of transmission and its biophysical constants. On the basis of the observed reaction of the differential pepper genotypes, two strains of this virus were identified.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes
T1  - Potato Virus Y (PVY) on pepper in Serbia
EP  - 549
IS  - 579
SP  - 545
DO  - 10.17660/ActaHortic.2002.579.96
ER  - 
@conference{
author = "Mijatović, Mirjana and Ivanović, Miroslav and Obradović, Aleksa and Zečević, Bogoljub",
year = "2002",
abstract = "During 1997, samples of plants showing symptoms of a viral disease were collected from thirteen pepper growing areas. On some plants characteristic symptoms of Potato Virus Y (PVY) infection were observed. Pepper samples were tested by applying the direct immunoenzyme test (DAS ELISA). For the identification of the virus a PVY antiserum was used, i.e. the kit of Loewe Biochemica, GmbH Germany. Isolation of the virus was performed by the method of mechanical inoculation using 0.01 M phosphate buffer (pH-7) and 0.1% Na2SO3 from the infected pepper samples. Identification of the virus strains was performed on the basis of the reaction of differential pepper genotypes. The virus was identified according to pepper disease symptoms, DAS ELISA test, reaction of inoculated test plants, way of transmission and its biophysical constants. On the basis of the observed reaction of the differential pepper genotypes, two strains of this virus were identified.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes",
title = "Potato Virus Y (PVY) on pepper in Serbia",
pages = "549-545",
number = "579",
doi = "10.17660/ActaHortic.2002.579.96"
}
Mijatović, M., Ivanović, M., Obradović, A.,& Zečević, B.. (2002). Potato Virus Y (PVY) on pepper in Serbia. in Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes
International Society for Horticultural Science.(579), 545-549.
https://doi.org/10.17660/ActaHortic.2002.579.96
Mijatović M, Ivanović M, Obradović A, Zečević B. Potato Virus Y (PVY) on pepper in Serbia. in Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes. 2002;(579):545-549.
doi:10.17660/ActaHortic.2002.579.96 .
Mijatović, Mirjana, Ivanović, Miroslav, Obradović, Aleksa, Zečević, Bogoljub, "Potato Virus Y (PVY) on pepper in Serbia" in Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes, no. 579 (2002):545-549,
https://doi.org/10.17660/ActaHortic.2002.579.96 . .
3
1
3

Population of bacteria infecting cauliflower in Yugoslavia

Obradović, Aleksa; Mijatović, Mirjana; Ivanović, Miroslav; Arsenijević, M.

(International Society for Horticultural Science, 2002)

TY  - CONF
AU  - Obradović, Aleksa
AU  - Mijatović, Mirjana
AU  - Ivanović, Miroslav
AU  - Arsenijević, M.
PY  - 2002
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/38
AB  - During the last several years, samples of diseased cauliflower plants were collected from different growing regions in Yugoslavia. Two types of symptoms were noticed: dark brown or black rot of cauliflower heads and V-shaped chlorosis along the leaf margin. Both types of symptoms were caused by phytopathogenic bacteria. Cultural, biochemical, physiological and serological characteristics of the strains isolated were studied. The results obtained indicated that several pathogenic bacteria, belonging to different genera, were responsible for the symptoms on cauliflower plants. According to the bacteriological characteristics, as causal agents of dark brown cauliflower head rot in Yugoslavia Pseudomonas viridiflava, P. cichorii and P. marginalis pv. marginalis were identified. However, from the soft rotted tissue the strains of Erwinia carotovora subsp. carotovora were isolated. Apart from bacteria belonging to genera Pseudomonas and Erwinia, Xanthomonas campestris pv. campestris was identified as a causal agent of cauliflower leaf chlorosis and black rot of vascular system.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Population of bacteria infecting cauliflower in Yugoslavia
EP  - 500
IS  - 579
SP  - 497
DO  - 10.17660/ActaHortic.2002.579.86
ER  - 
@conference{
author = "Obradović, Aleksa and Mijatović, Mirjana and Ivanović, Miroslav and Arsenijević, M.",
year = "2002",
abstract = "During the last several years, samples of diseased cauliflower plants were collected from different growing regions in Yugoslavia. Two types of symptoms were noticed: dark brown or black rot of cauliflower heads and V-shaped chlorosis along the leaf margin. Both types of symptoms were caused by phytopathogenic bacteria. Cultural, biochemical, physiological and serological characteristics of the strains isolated were studied. The results obtained indicated that several pathogenic bacteria, belonging to different genera, were responsible for the symptoms on cauliflower plants. According to the bacteriological characteristics, as causal agents of dark brown cauliflower head rot in Yugoslavia Pseudomonas viridiflava, P. cichorii and P. marginalis pv. marginalis were identified. However, from the soft rotted tissue the strains of Erwinia carotovora subsp. carotovora were isolated. Apart from bacteria belonging to genera Pseudomonas and Erwinia, Xanthomonas campestris pv. campestris was identified as a causal agent of cauliflower leaf chlorosis and black rot of vascular system.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Population of bacteria infecting cauliflower in Yugoslavia",
pages = "500-497",
number = "579",
doi = "10.17660/ActaHortic.2002.579.86"
}
Obradović, A., Mijatović, M., Ivanović, M.,& Arsenijević, M.. (2002). Population of bacteria infecting cauliflower in Yugoslavia. in Acta Horticulturae
International Society for Horticultural Science.(579), 497-500.
https://doi.org/10.17660/ActaHortic.2002.579.86
Obradović A, Mijatović M, Ivanović M, Arsenijević M. Population of bacteria infecting cauliflower in Yugoslavia. in Acta Horticulturae. 2002;(579):497-500.
doi:10.17660/ActaHortic.2002.579.86 .
Obradović, Aleksa, Mijatović, Mirjana, Ivanović, Miroslav, Arsenijević, M., "Population of bacteria infecting cauliflower in Yugoslavia" in Acta Horticulturae, no. 579 (2002):497-500,
https://doi.org/10.17660/ActaHortic.2002.579.86 . .
2
1

New occurrence of Xanthomonas campestris pv. campestris as a pathogen of some Brassica spp. in Yugoslavia

Obradović, Aleksa; Arsenijević, M.; Ivanović, Miroslav; Sretenović-Rajičić, Tatjana

(Springer, Dordrecht, 2001)

TY  - CONF
AU  - Obradović, Aleksa
AU  - Arsenijević, M.
AU  - Ivanović, Miroslav
AU  - Sretenović-Rajičić, Tatjana
PY  - 2001
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/36
AB  - Cabbage (Brassica oleracea var. capitata L.) is one of major vegetable species grown in Yugoslavia. The other Brassica spp. are grown in this country as well, but regarding the planted area they are considered as minor crops. However, the number of fields planted with cauliflower (B. o. var. botrytis L.) and kale (B. o. var. sabauda L.) increased recently.
PB  - Springer, Dordrecht
C3  - Plant Pathogenic Bacteria
T1  - New occurrence of Xanthomonas campestris pv. campestris as a pathogen of some Brassica spp. in Yugoslavia
EP  - 254
SP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_360
ER  - 
@conference{
author = "Obradović, Aleksa and Arsenijević, M. and Ivanović, Miroslav and Sretenović-Rajičić, Tatjana",
year = "2001",
abstract = "Cabbage (Brassica oleracea var. capitata L.) is one of major vegetable species grown in Yugoslavia. The other Brassica spp. are grown in this country as well, but regarding the planted area they are considered as minor crops. However, the number of fields planted with cauliflower (B. o. var. botrytis L.) and kale (B. o. var. sabauda L.) increased recently.",
publisher = "Springer, Dordrecht",
journal = "Plant Pathogenic Bacteria",
title = "New occurrence of Xanthomonas campestris pv. campestris as a pathogen of some Brassica spp. in Yugoslavia",
pages = "254-252",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_360"
}
Obradović, A., Arsenijević, M., Ivanović, M.,& Sretenović-Rajičić, T.. (2001). New occurrence of Xanthomonas campestris pv. campestris as a pathogen of some Brassica spp. in Yugoslavia. in Plant Pathogenic Bacteria
Springer, Dordrecht., 252-254.
https://hdl.handle.net/21.15107/rcub_agrospace_360
Obradović A, Arsenijević M, Ivanović M, Sretenović-Rajičić T. New occurrence of Xanthomonas campestris pv. campestris as a pathogen of some Brassica spp. in Yugoslavia. in Plant Pathogenic Bacteria. 2001;:252-254.
https://hdl.handle.net/21.15107/rcub_agrospace_360 .
Obradović, Aleksa, Arsenijević, M., Ivanović, Miroslav, Sretenović-Rajičić, Tatjana, "New occurrence of Xanthomonas campestris pv. campestris as a pathogen of some Brassica spp. in Yugoslavia" in Plant Pathogenic Bacteria (2001):252-254,
https://hdl.handle.net/21.15107/rcub_agrospace_360 .

Sudden appearance of the tomato race of Xanthomonas campestris pv. vesicatoria in Yugoslavia

Obradović, Aleksa; Mavridis, A; Rudolph, K; Zdravković, Jasmina

(Springer, Dordrecht, 2001)

TY  - CONF
AU  - Obradović, Aleksa
AU  - Mavridis, A
AU  - Rudolph, K
AU  - Zdravković, Jasmina
PY  - 2001
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/35
AB  - In 1957, Sutic identified Pseudomonas vesicatoria (Doidge) Stevens as the most important bacterial pathogen of tomato (Lycopersicon esculentum Mill.) grown in Yugoslavia (12). However, since that time this bacterium has not been reported as a tomato pathogen in this country.
PB  - Springer, Dordrecht
C3  - Plant Pathogenic Bacteria
T1  - Sudden appearance of the tomato race of Xanthomonas campestris pv. vesicatoria in Yugoslavia
EP  - 352
SP  - 350
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_340
ER  - 
@conference{
author = "Obradović, Aleksa and Mavridis, A and Rudolph, K and Zdravković, Jasmina",
year = "2001",
abstract = "In 1957, Sutic identified Pseudomonas vesicatoria (Doidge) Stevens as the most important bacterial pathogen of tomato (Lycopersicon esculentum Mill.) grown in Yugoslavia (12). However, since that time this bacterium has not been reported as a tomato pathogen in this country.",
publisher = "Springer, Dordrecht",
journal = "Plant Pathogenic Bacteria",
title = "Sudden appearance of the tomato race of Xanthomonas campestris pv. vesicatoria in Yugoslavia",
pages = "352-350",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_340"
}
Obradović, A., Mavridis, A., Rudolph, K.,& Zdravković, J.. (2001). Sudden appearance of the tomato race of Xanthomonas campestris pv. vesicatoria in Yugoslavia. in Plant Pathogenic Bacteria
Springer, Dordrecht., 350-352.
https://hdl.handle.net/21.15107/rcub_agrospace_340
Obradović A, Mavridis A, Rudolph K, Zdravković J. Sudden appearance of the tomato race of Xanthomonas campestris pv. vesicatoria in Yugoslavia. in Plant Pathogenic Bacteria. 2001;:350-352.
https://hdl.handle.net/21.15107/rcub_agrospace_340 .
Obradović, Aleksa, Mavridis, A, Rudolph, K, Zdravković, Jasmina, "Sudden appearance of the tomato race of Xanthomonas campestris pv. vesicatoria in Yugoslavia" in Plant Pathogenic Bacteria (2001):350-352,
https://hdl.handle.net/21.15107/rcub_agrospace_340 .
1

Bacterial diseases of pepper in Yugoslavia

Obradović, Aleksa; Mavridis, A; Rudolph, K; Arsenijević, M.; Mijatović, Mirjana

(Springer, Dordrecht, 2001)

TY  - CONF
AU  - Obradović, Aleksa
AU  - Mavridis, A
AU  - Rudolph, K
AU  - Arsenijević, M.
AU  - Mijatović, Mirjana
PY  - 2001
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/34
AB  - During the last decade, bacterial diseases appeared to be a serious problem in the production of pepper (Capsicum annuum L) in Yugoslavia (3, 8). In some years, numerous greasy or necrotic spots and blight symptoms were observed on cotyledons of pepper transplants grown in hotbeds and plastic tunnels at the beginning of the season. At the end of June and beginning of July, small necrotic spots, surrounded by a weak halo, were frequently noticed on the leaves of pepper grown in an open field. These spots tend to coalesce, causing leaf blight and premature abscission. Scabby spots, irregular in shape, were rarely noticed on diseased fruits. However, soft rot of pepper fruits usually appeared after heavy rainfall followed by wind and hail.
PB  - Springer, Dordrecht
C3  - Plant Pathogenic Bacteria
T1  - Bacterial diseases of pepper in Yugoslavia
EP  - 258
SP  - 255
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_341
ER  - 
@conference{
author = "Obradović, Aleksa and Mavridis, A and Rudolph, K and Arsenijević, M. and Mijatović, Mirjana",
year = "2001",
abstract = "During the last decade, bacterial diseases appeared to be a serious problem in the production of pepper (Capsicum annuum L) in Yugoslavia (3, 8). In some years, numerous greasy or necrotic spots and blight symptoms were observed on cotyledons of pepper transplants grown in hotbeds and plastic tunnels at the beginning of the season. At the end of June and beginning of July, small necrotic spots, surrounded by a weak halo, were frequently noticed on the leaves of pepper grown in an open field. These spots tend to coalesce, causing leaf blight and premature abscission. Scabby spots, irregular in shape, were rarely noticed on diseased fruits. However, soft rot of pepper fruits usually appeared after heavy rainfall followed by wind and hail.",
publisher = "Springer, Dordrecht",
journal = "Plant Pathogenic Bacteria",
title = "Bacterial diseases of pepper in Yugoslavia",
pages = "258-255",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_341"
}
Obradović, A., Mavridis, A., Rudolph, K., Arsenijević, M.,& Mijatović, M.. (2001). Bacterial diseases of pepper in Yugoslavia. in Plant Pathogenic Bacteria
Springer, Dordrecht., 255-258.
https://hdl.handle.net/21.15107/rcub_agrospace_341
Obradović A, Mavridis A, Rudolph K, Arsenijević M, Mijatović M. Bacterial diseases of pepper in Yugoslavia. in Plant Pathogenic Bacteria. 2001;:255-258.
https://hdl.handle.net/21.15107/rcub_agrospace_341 .
Obradović, Aleksa, Mavridis, A, Rudolph, K, Arsenijević, M., Mijatović, Mirjana, "Bacterial diseases of pepper in Yugoslavia" in Plant Pathogenic Bacteria (2001):255-258,
https://hdl.handle.net/21.15107/rcub_agrospace_341 .
6

Disease intensity of some tomato viroses in Serbia

Mijatović, Mirjana; Zdravković, Jasmina; Marković, Živoslav; Obradović, Aleksa

(Polish Academy of Sciences, 2000)

TY  - JOUR
AU  - Mijatović, Mirjana
AU  - Zdravković, Jasmina
AU  - Marković, Živoslav
AU  - Obradović, Aleksa
PY  - 2000
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/33
AB  - In this paper we present the data on the disease intensity of the tomato plants grown in glass and plastic-houses, and in the open field. The infection was caused by the following viruses: Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), Tomato spotted wilt virus (TSWV), Alfalfa mosaic virus (AMV), Potato virus X (PVX), Potato virus Y (PVY), Tomato black ring virus (TBRV), Tomato ringspot virus (ToRSV), Tomato aspermy virus (TAV), and Cucumber mosaic virus (CMV). These viruses represented most frequent tomato pathogens in Serbia. According to the obtained results, it could be concluded that 92.94% of the tested tomato plants grown in glass and plastic-houses, and 89.82% grown in the open field were infected by one of the above viruses. Most of the plant samples were infected by two or more viruses. The most frequent viruses - tomato pathogens in Serbia were ToMV, PVY and TMV.
PB  - Polish Academy of Sciences
T2  - Acta Physiologiae Plantarum
T1  - Disease intensity of some tomato viroses in Serbia
EP  - 335
IS  - 3
SP  - 332
VL  - 22
DO  - 10.1007/s11738-000-0049-7
ER  - 
@article{
author = "Mijatović, Mirjana and Zdravković, Jasmina and Marković, Živoslav and Obradović, Aleksa",
year = "2000",
abstract = "In this paper we present the data on the disease intensity of the tomato plants grown in glass and plastic-houses, and in the open field. The infection was caused by the following viruses: Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), Tomato spotted wilt virus (TSWV), Alfalfa mosaic virus (AMV), Potato virus X (PVX), Potato virus Y (PVY), Tomato black ring virus (TBRV), Tomato ringspot virus (ToRSV), Tomato aspermy virus (TAV), and Cucumber mosaic virus (CMV). These viruses represented most frequent tomato pathogens in Serbia. According to the obtained results, it could be concluded that 92.94% of the tested tomato plants grown in glass and plastic-houses, and 89.82% grown in the open field were infected by one of the above viruses. Most of the plant samples were infected by two or more viruses. The most frequent viruses - tomato pathogens in Serbia were ToMV, PVY and TMV.",
publisher = "Polish Academy of Sciences",
journal = "Acta Physiologiae Plantarum",
title = "Disease intensity of some tomato viroses in Serbia",
pages = "335-332",
number = "3",
volume = "22",
doi = "10.1007/s11738-000-0049-7"
}
Mijatović, M., Zdravković, J., Marković, Ž.,& Obradović, A.. (2000). Disease intensity of some tomato viroses in Serbia. in Acta Physiologiae Plantarum
Polish Academy of Sciences., 22(3), 332-335.
https://doi.org/10.1007/s11738-000-0049-7
Mijatović M, Zdravković J, Marković Ž, Obradović A. Disease intensity of some tomato viroses in Serbia. in Acta Physiologiae Plantarum. 2000;22(3):332-335.
doi:10.1007/s11738-000-0049-7 .
Mijatović, Mirjana, Zdravković, Jasmina, Marković, Živoslav, Obradović, Aleksa, "Disease intensity of some tomato viroses in Serbia" in Acta Physiologiae Plantarum, 22, no. 3 (2000):332-335,
https://doi.org/10.1007/s11738-000-0049-7 . .
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