TY  - JOUR
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Kojić, S.
AU  - Pivić, R.
AU  - Stanojković-Sebić, A.
AU  - Zdravković, Milan
AU  - Pavlović, Snežana
PY  - 2015
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/173
AB  - Sweet William (Dianthus barbatus, Caryophyllaceae) is a biennial or short-lived perennial plant native to southern Europe, from the Pyrenees to the Carpathians and the Balkans. During the summers of 2012 and 2013, phytoplasma-like symptoms were observed on D. barbatus plants on a Serbian plantation (Pancevo, 44°51′49″ N, 20°39′33″ E, 80 m ASL). Only seven symptomatic plants were observed in the summer of 2012. Disease incidence in 2013 was estimated to be less than 1% but increased during 2014 to 4%. Affected plants, showing symptoms of leaf reddening, malformation, and proliferation; flower bud deficiency; and abnormal shoot production, were tested for phytoplasmas. Samples were collected from seven symptomatic and three symptomless plants each year (20 samples), and total nucleic acid was extracted from midrib tissue using a method that includes a phytoplasma enrichment step and DNA purification by chloroform/phenol (3). Oligonucleotide primers specific to the phytoplasma 16S to 23S rRNA intergenic spacer region were used in polymerase chain reaction (PCR) assays on DNA extracted from Sweet William plants (1,3). Using phytoplasma universal primer pairs P1/P7 and P1/16S-Sr, phytoplasma-specific 1.8- and 1.5-kb amplicons were obtained from four and six symptomatic plants collected in 2012 and 2013, respectively. Nested PCR with R16F2n/R2 primers yielded ~1.2-kb amplicons from DNAs of all symptomatic plants (1). No amplicon was generated in PCRs conducted with DNA templates from symptomless plants. Restriction fragment length polymorphism (RFLP) analysis of amplified 1.2-kb fragments was performed using four endonucleases (AluI, Tru1I, HhaI, and HpaII). Comparative analysis was done using RFLP patterns of Stolbur (Stol), Aster Yellows (AY), Flavescence Doree-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas. PCR-RFLP patterns from tested samples were identical to those of the Stol reference strain, indicating that diseased Sweet William was affected by phytoplasma belonging to the 16SrXII-A (Stolbur) group. The sequence of a 1.2-kb rDNA PCR product derived from sample Tk9 (deposited under accession number KM401436 in NCBI GenBank) showed the closest identity (100%) to those of Bulgarian corn (KF907506.1), Iranian ‘Bois Noir’ (KJ637208.1), and two Serbian phytoplasmas (KJ174507.1 from Calendula officinalis and KF614623.1 from Paeonia tenuifolia), all belonging to the ‘Candidatus Phytoplasma solani’ Stolbur subgroup. Previously, Aster Yellows Phytoplasma (16SrI) had been detected in two Dianthus species: D. barbatus (Sweet William) and D. caryophyllus (carnation) (2). This is the first record of the 16SrXII-A phytoplasma subgroup being associated with yellowing and reddening of D. barbatus in Serbia. The Stolbur phytoplasma occurrence on Sweet William is significant for the management of the disease in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.
EP  - 283
IS  - 2
SP  - 283
VL  - 99
DO  - 10.1094/PDIS-08-14-0875-PDN
ER  - 

@article{
author = "Jošić, Dragana and Starović, Mira and Kojić, S. and Pivić, R. and Stanojković-Sebić, A. and Zdravković, Milan and Pavlović, Snežana",
year = "2015",
abstract = "Sweet William (Dianthus barbatus, Caryophyllaceae) is a biennial or short-lived perennial plant native to southern Europe, from the Pyrenees to the Carpathians and the Balkans. During the summers of 2012 and 2013, phytoplasma-like symptoms were observed on D. barbatus plants on a Serbian plantation (Pancevo, 44°51′49″ N, 20°39′33″ E, 80 m ASL). Only seven symptomatic plants were observed in the summer of 2012. Disease incidence in 2013 was estimated to be less than 1% but increased during 2014 to 4%. Affected plants, showing symptoms of leaf reddening, malformation, and proliferation; flower bud deficiency; and abnormal shoot production, were tested for phytoplasmas. Samples were collected from seven symptomatic and three symptomless plants each year (20 samples), and total nucleic acid was extracted from midrib tissue using a method that includes a phytoplasma enrichment step and DNA purification by chloroform/phenol (3). Oligonucleotide primers specific to the phytoplasma 16S to 23S rRNA intergenic spacer region were used in polymerase chain reaction (PCR) assays on DNA extracted from Sweet William plants (1,3). Using phytoplasma universal primer pairs P1/P7 and P1/16S-Sr, phytoplasma-specific 1.8- and 1.5-kb amplicons were obtained from four and six symptomatic plants collected in 2012 and 2013, respectively. Nested PCR with R16F2n/R2 primers yielded ~1.2-kb amplicons from DNAs of all symptomatic plants (1). No amplicon was generated in PCRs conducted with DNA templates from symptomless plants. Restriction fragment length polymorphism (RFLP) analysis of amplified 1.2-kb fragments was performed using four endonucleases (AluI, Tru1I, HhaI, and HpaII). Comparative analysis was done using RFLP patterns of Stolbur (Stol), Aster Yellows (AY), Flavescence Doree-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas. PCR-RFLP patterns from tested samples were identical to those of the Stol reference strain, indicating that diseased Sweet William was affected by phytoplasma belonging to the 16SrXII-A (Stolbur) group. The sequence of a 1.2-kb rDNA PCR product derived from sample Tk9 (deposited under accession number KM401436 in NCBI GenBank) showed the closest identity (100%) to those of Bulgarian corn (KF907506.1), Iranian ‘Bois Noir’ (KJ637208.1), and two Serbian phytoplasmas (KJ174507.1 from Calendula officinalis and KF614623.1 from Paeonia tenuifolia), all belonging to the ‘Candidatus Phytoplasma solani’ Stolbur subgroup. Previously, Aster Yellows Phytoplasma (16SrI) had been detected in two Dianthus species: D. barbatus (Sweet William) and D. caryophyllus (carnation) (2). This is the first record of the 16SrXII-A phytoplasma subgroup being associated with yellowing and reddening of D. barbatus in Serbia. The Stolbur phytoplasma occurrence on Sweet William is significant for the management of the disease in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.",
pages = "283-283",
number = "2",
volume = "99",
doi = "10.1094/PDIS-08-14-0875-PDN"
}

Jošić, D., Starović, M., Kojić, S., Pivić, R., Stanojković-Sebić, A., Zdravković, M.,& Pavlović, S.. (2015). Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(2), 283-283.
https://doi.org/10.1094/PDIS-08-14-0875-PDN

Jošić D, Starović M, Kojić S, Pivić R, Stanojković-Sebić A, Zdravković M, Pavlović S. Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.. in Plant Disease. 2015;99(2):283-283.
doi:10.1094/PDIS-08-14-0875-PDN .

Jošić, Dragana, Starović, Mira, Kojić, S., Pivić, R., Stanojković-Sebić, A., Zdravković, Milan, Pavlović, Snežana, "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia." in Plant Disease, 99, no. 2 (2015):283-283,
https://doi.org/10.1094/PDIS-08-14-0875-PDN . .

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Starović, Mira
AU  - Stojanović, S.
AU  - Aleksić, G.
AU  - Kojić, S.
AU  - Zdravković, Milan
AU  - Jošić, Dragana
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/162
AB  - Pot marigold (Calendula officinalis L.) is native to southern Europe. Compounds of marigold flowers exhibit anti-inflammatory, anti-tumor-promoting, and cytotoxic activities (4). In Serbia, pot marigold is cultivated as an important medicinal and ornamental plant. Typical phyllody, virescence, proliferation of axillary buds, and witches' broom symptoms were sporadically observed in 2011 in Pancevo plantation, Serbia (44°51′49″ N, 20°39′33″ E, 80 m above sea level). Until 2013, the number of uniformly distributed affected pot marigold plants reached 20% in the field. Due to the lack of seed production, profitability of the cultivation was seriously affected. Leaf samples from 10 symptomatic and 4 symptomless marigold plants were collected and total nucleic acid was extracted from midrib tissue (3). Direct PCR and nested PCR were carried out with primer pairs P1/16S-SR and R16F2n/R16R2n, respectively (3). Amplicons 1.5 and 1.2 kb in length, specific for the 16S rRNA gene, were amplified in all symptomatic plants. No PCR products were obtained when DNA isolated from symptomless plants was used. Restriction fragment length polymorphism (RFLP) patterns of the 1.2-kb fragments of 16S rDNA were determined by digestion with four endonucleases separately (TruI1, AluI, HpaII, and HhaI) and compared with those of Stolbur (Stol), Aster Yellows (AY), Flavescence dorée-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas (2). RFLP patterns from all symptomatic pot marigold plants were identical to the Stol pattern, indicating Stolbur phytoplasma presence in affected plants. The 1.2-kb amplicon of representative Nv8 strain was sequenced and the data were submitted to GenBank (accession no. KJ174507). BLASTn analysis of the sequence was compared with sequences available in GenBank, showing 100% identity with 16S rRNA gene of strains from Paeonia tenuifolia (KF614623) and corn (JQ730750) from Serbia, and peach (KF263684) from Iran. All of these are members of the 16SrXII ‘Candidatus Phytoplasma solani’ group, subgroup A (Stolbur). Phytoplasmas belonging to aster yellows (16SrI) (Italy and Canada) and peanut witches' broom related phytoplasma (16SrII) group (Iran) have been identified in diseased pot marigold plants (1). To our knowledge, this is the first report of natural infection of pot marigold by Stolbur phytoplasma in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.
EP  - 1152
IS  - 8
SP  - 1152
VL  - 98
DO  - 10.1094/PDIS-01-14-0085-PDN
ER  - 

@article{
author = "Pavlović, Snežana and Starović, Mira and Stojanović, S. and Aleksić, G. and Kojić, S. and Zdravković, Milan and Jošić, Dragana",
year = "2014",
abstract = "Pot marigold (Calendula officinalis L.) is native to southern Europe. Compounds of marigold flowers exhibit anti-inflammatory, anti-tumor-promoting, and cytotoxic activities (4). In Serbia, pot marigold is cultivated as an important medicinal and ornamental plant. Typical phyllody, virescence, proliferation of axillary buds, and witches' broom symptoms were sporadically observed in 2011 in Pancevo plantation, Serbia (44°51′49″ N, 20°39′33″ E, 80 m above sea level). Until 2013, the number of uniformly distributed affected pot marigold plants reached 20% in the field. Due to the lack of seed production, profitability of the cultivation was seriously affected. Leaf samples from 10 symptomatic and 4 symptomless marigold plants were collected and total nucleic acid was extracted from midrib tissue (3). Direct PCR and nested PCR were carried out with primer pairs P1/16S-SR and R16F2n/R16R2n, respectively (3). Amplicons 1.5 and 1.2 kb in length, specific for the 16S rRNA gene, were amplified in all symptomatic plants. No PCR products were obtained when DNA isolated from symptomless plants was used. Restriction fragment length polymorphism (RFLP) patterns of the 1.2-kb fragments of 16S rDNA were determined by digestion with four endonucleases separately (TruI1, AluI, HpaII, and HhaI) and compared with those of Stolbur (Stol), Aster Yellows (AY), Flavescence dorée-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas (2). RFLP patterns from all symptomatic pot marigold plants were identical to the Stol pattern, indicating Stolbur phytoplasma presence in affected plants. The 1.2-kb amplicon of representative Nv8 strain was sequenced and the data were submitted to GenBank (accession no. KJ174507). BLASTn analysis of the sequence was compared with sequences available in GenBank, showing 100% identity with 16S rRNA gene of strains from Paeonia tenuifolia (KF614623) and corn (JQ730750) from Serbia, and peach (KF263684) from Iran. All of these are members of the 16SrXII ‘Candidatus Phytoplasma solani’ group, subgroup A (Stolbur). Phytoplasmas belonging to aster yellows (16SrI) (Italy and Canada) and peanut witches' broom related phytoplasma (16SrII) group (Iran) have been identified in diseased pot marigold plants (1). To our knowledge, this is the first report of natural infection of pot marigold by Stolbur phytoplasma in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.",
pages = "1152-1152",
number = "8",
volume = "98",
doi = "10.1094/PDIS-01-14-0085-PDN"
}

Pavlović, S., Starović, M., Stojanović, S., Aleksić, G., Kojić, S., Zdravković, M.,& Jošić, D.. (2014). The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1152-1152.
https://doi.org/10.1094/PDIS-01-14-0085-PDN

Pavlović S, Starović M, Stojanović S, Aleksić G, Kojić S, Zdravković M, Jošić D. The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.. in Plant Disease. 2014;98(8):1152-1152.
doi:10.1094/PDIS-01-14-0085-PDN .

Pavlović, Snežana, Starović, Mira, Stojanović, S., Aleksić, G., Kojić, S., Zdravković, Milan, Jošić, Dragana, "The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia." in Plant Disease, 98, no. 8 (2014):1152-1152,
https://doi.org/10.1094/PDIS-01-14-0085-PDN . .