TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/143
AB  - A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)
EP  - 406
IS  - 3
SP  - 397
VL  - 113
DO  - 10.1007/s11240-012-0279-6
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Vinterhalter, Dragan and Zdravković, Jasmina and Cvikić, Dejan and Mitić, Nevena",
year = "2013",
abstract = "A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)",
pages = "406-397",
number = "3",
volume = "113",
doi = "10.1007/s11240-012-0279-6"
}

Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Vinterhalter, D., Zdravković, J., Cvikić, D.,& Mitić, N.. (2013). Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 113(3), 397-406.
https://doi.org/10.1007/s11240-012-0279-6

Pavlović S, Vinterhalter B, Zdravković-Korać S, Vinterhalter D, Zdravković J, Cvikić D, Mitić N. Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture. 2013;113(3):397-406.
doi:10.1007/s11240-012-0279-6 .

Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Vinterhalter, Dragan, Zdravković, Jasmina, Cvikić, Dejan, Mitić, Nevena, "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)" in Plant Cell Tissue and Organ Culture, 113, no. 3 (2013):397-406,
https://doi.org/10.1007/s11240-012-0279-6 . .

TY  - CONF
AU  - Pavlović, Suzana
AU  - Adžić, Slađan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/139
AB  - An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.
EP  - 246
SP  - 239
VL  - 1005
DO  - 10.17660/ActaHortic.2013.1005.26
ER  - 

@conference{
author = "Pavlović, Suzana and Adžić, Slađan and Zdravković, Jasmina and Cvikić, Dejan and Milojević, Jelena and Vinterhalter, Branka and Mitić, Nevena",
year = "2013",
abstract = "An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.",
pages = "246-239",
volume = "1005",
doi = "10.17660/ActaHortic.2013.1005.26"
}

Pavlović, S., Adžić, S., Zdravković, J., Cvikić, D., Milojević, J., Vinterhalter, B.,& Mitić, N.. (2013). Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae
International Society for Horticultural Science., 1005, 239-246.
https://doi.org/10.17660/ActaHortic.2013.1005.26

Pavlović S, Adžić S, Zdravković J, Cvikić D, Milojević J, Vinterhalter B, Mitić N. Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae. 2013;1005:239-246.
doi:10.17660/ActaHortic.2013.1005.26 .

Pavlović, Suzana, Adžić, Slađan, Zdravković, Jasmina, Cvikić, Dejan, Milojević, Jelena, Vinterhalter, Branka, Mitić, Nevena, "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L." in Acta Horticulturae, 1005 (2013):239-246,
https://doi.org/10.17660/ActaHortic.2013.1005.26 . .

TY  - JOUR
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Pavlović, Suzana
AU  - Mitić, Nevena
AU  - Calić, Dušica
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/123
AB  - As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Long days promote somatic embryogenesis in spinach
EP  - 37
SP  - 32
VL  - 142
DO  - 10.1016/j.scienta.2012.04.020
ER  - 

@article{
author = "Milojević, Jelena and Tubić, Ljiljana and Pavlović, Suzana and Mitić, Nevena and Calić, Dušica and Vinterhalter, Branka and Zdravković-Korać, Snežana",
year = "2012",
abstract = "As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Long days promote somatic embryogenesis in spinach",
pages = "37-32",
volume = "142",
doi = "10.1016/j.scienta.2012.04.020"
}

Milojević, J., Tubić, L., Pavlović, S., Mitić, N., Calić, D., Vinterhalter, B.,& Zdravković-Korać, S.. (2012). Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 142, 32-37.
https://doi.org/10.1016/j.scienta.2012.04.020

Milojević J, Tubić L, Pavlović S, Mitić N, Calić D, Vinterhalter B, Zdravković-Korać S. Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae. 2012;142:32-37.
doi:10.1016/j.scienta.2012.04.020 .

Milojević, Jelena, Tubić, Ljiljana, Pavlović, Suzana, Mitić, Nevena, Calić, Dušica, Vinterhalter, Branka, Zdravković-Korać, Snežana, "Long days promote somatic embryogenesis in spinach" in Scientia Horticulturae, 142 (2012):32-37,
https://doi.org/10.1016/j.scienta.2012.04.020 . .

TY  - CONF
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Adžić, Slađan
AU  - Đorđević, Mladen
AU  - Ugrinović, Milan
AU  - Mitić, Nevena
PY  - 2011
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/577
AB  - Cauliflower (Brassica oleracea var. botrytis) is an important vegetable plant grown worldwide that is impacted by biotic and abiotic stresses. Plant biotechnology could be an efficient approach to enhance stress tolerance. Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as biotic and abiotic stress response. Thus, we have established an Agrobaterium tumefacens-mediated protocol for transformation of cauliflower (line KFRM4) using the binary vector pSHX004 with the mitogen-activated protein kinase kinase kinase (MAPKKK) gene from tobacco (NPK1) for stress tolerance and the phosphinothricin acetyltransferase (bar) selectable marker gene for herbicide resistance. The effect of acetosyringone (AS) on transformation efficiency was also investigated. Hypocotyls of 8 day-old seedlings, pre-cultivated on MS shoot induction medium (SIM) supplemented with 1.0 mg/l BA and 0.2 mg/l IBA for 3 days, were infected with bacterial suspension containing 0 or 100 μM AS and than co-cultivated on SIM with the same concentration of AS as infection medium for 3 days. Upon co-cultivation, the explants were cultured on SIM containing  500 mg/l cefotaxime for 8 days, and than subcultured on SIM selection media with 5 mg/l phosphinothricin (PPT) until shoot regeneration. The addition of AS during co-cultivation increased transformation frequency (7.8% vs. 1.4% in its absence). Selected PPT-resistant shoots were continuously maintained and multiplied on MS selection medium containing 2 mg/l PPT and BA or Kin (0.5-1.0 mg/l) alone or in combination with IBA (0.1-0.2 mg/l) displaying vigorous growth and development. Integration of the bar gene in transgenic shoots was verified by chlorophenol red (CR) assay while PCR confirmed the presence of NPK1 gene. Transformed plants provide a promising material for stress tolerance study.
PB  - Beograd : Serbian Plant Physiology Society
PB  - Beograd : Institute for Biological Research "Siniša Stanković"
C3  - 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts
T1  - Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_rivec_577
ER  - 

@conference{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Adžić, Slađan and Đorđević, Mladen and Ugrinović, Milan and Mitić, Nevena",
year = "2011",
abstract = "Cauliflower (Brassica oleracea var. botrytis) is an important vegetable plant grown worldwide that is impacted by biotic and abiotic stresses. Plant biotechnology could be an efficient approach to enhance stress tolerance. Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as biotic and abiotic stress response. Thus, we have established an Agrobaterium tumefacens-mediated protocol for transformation of cauliflower (line KFRM4) using the binary vector pSHX004 with the mitogen-activated protein kinase kinase kinase (MAPKKK) gene from tobacco (NPK1) for stress tolerance and the phosphinothricin acetyltransferase (bar) selectable marker gene for herbicide resistance. The effect of acetosyringone (AS) on transformation efficiency was also investigated. Hypocotyls of 8 day-old seedlings, pre-cultivated on MS shoot induction medium (SIM) supplemented with 1.0 mg/l BA and 0.2 mg/l IBA for 3 days, were infected with bacterial suspension containing 0 or 100 μM AS and than co-cultivated on SIM with the same concentration of AS as infection medium for 3 days. Upon co-cultivation, the explants were cultured on SIM containing  500 mg/l cefotaxime for 8 days, and than subcultured on SIM selection media with 5 mg/l phosphinothricin (PPT) until shoot regeneration. The addition of AS during co-cultivation increased transformation frequency (7.8% vs. 1.4% in its absence). Selected PPT-resistant shoots were continuously maintained and multiplied on MS selection medium containing 2 mg/l PPT and BA or Kin (0.5-1.0 mg/l) alone or in combination with IBA (0.1-0.2 mg/l) displaying vigorous growth and development. Integration of the bar gene in transgenic shoots was verified by chlorophenol red (CR) assay while PCR confirmed the presence of NPK1 gene. Transformed plants provide a promising material for stress tolerance study.",
publisher = "Beograd : Serbian Plant Physiology Society, Beograd : Institute for Biological Research "Siniša Stanković"",
journal = "1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts",
title = "Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_rivec_577"
}

Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Adžić, S., Đorđević, M., Ugrinović, M.,& Mitić, N.. (2011). Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance. in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts
Beograd : Serbian Plant Physiology Society., 64.
https://hdl.handle.net/21.15107/rcub_rivec_577

Pavlović S, Vinterhalter B, Zdravković-Korać S, Adžić S, Đorđević M, Ugrinović M, Mitić N. Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance. in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts. 2011;:64.
https://hdl.handle.net/21.15107/rcub_rivec_577 .

Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Adžić, Slađan, Đorđević, Mladen, Ugrinović, Milan, Mitić, Nevena, "Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance" in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts (2011):64,
https://hdl.handle.net/21.15107/rcub_rivec_577 .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Mitić, Nevena
AU  - Adžić, Slađan
AU  - Pavlović, Nenad
AU  - Zdravković, Milan
AU  - Vinterhalter, Dragan
PY  - 2010
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/86
AB  - Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse.
AB  - Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower
T1  - Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola
EP  - 345
IS  - 2
SP  - 337
VL  - 62
DO  - 10.2298/ABS1002337P
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Mitić, Nevena and Adžić, Slađan and Pavlović, Nenad and Zdravković, Milan and Vinterhalter, Dragan",
year = "2010",
abstract = "Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse., Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower, Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola",
pages = "345-337",
number = "2",
volume = "62",
doi = "10.2298/ABS1002337P"
}

Pavlović, S., Vinterhalter, B., Mitić, N., Adžić, S., Pavlović, N., Zdravković, M.,& Vinterhalter, D.. (2010). In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 337-345.
https://doi.org/10.2298/ABS1002337P

Pavlović S, Vinterhalter B, Mitić N, Adžić S, Pavlović N, Zdravković M, Vinterhalter D. In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower. in Archives of Biological Sciences. 2010;62(2):337-345.
doi:10.2298/ABS1002337P .

Pavlović, Suzana, Vinterhalter, Branka, Mitić, Nevena, Adžić, Slađan, Pavlović, Nenad, Zdravković, Milan, Vinterhalter, Dragan, "In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower" in Archives of Biological Sciences, 62, no. 2 (2010):337-345,
https://doi.org/10.2298/ABS1002337P . .