TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/465
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
C3  - Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
EP  - 213
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_rivec_465
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213-213",
url = "https://hdl.handle.net/21.15107/rcub_rivec_465"
}

Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
East Sarajevo : Faculty of Agriculture., 213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465

Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10. 2021;:213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465 .

Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 (2021):213-213,
https://hdl.handle.net/21.15107/rcub_rivec_465 .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/143
AB  - A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)
EP  - 406
IS  - 3
SP  - 397
VL  - 113
DO  - 10.1007/s11240-012-0279-6
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Vinterhalter, Dragan and Zdravković, Jasmina and Cvikić, Dejan and Mitić, Nevena",
year = "2013",
abstract = "A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)",
pages = "406-397",
number = "3",
volume = "113",
doi = "10.1007/s11240-012-0279-6"
}

Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Vinterhalter, D., Zdravković, J., Cvikić, D.,& Mitić, N.. (2013). Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 113(3), 397-406.
https://doi.org/10.1007/s11240-012-0279-6

Pavlović S, Vinterhalter B, Zdravković-Korać S, Vinterhalter D, Zdravković J, Cvikić D, Mitić N. Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture. 2013;113(3):397-406.
doi:10.1007/s11240-012-0279-6 .

Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Vinterhalter, Dragan, Zdravković, Jasmina, Cvikić, Dejan, Mitić, Nevena, "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)" in Plant Cell Tissue and Organ Culture, 113, no. 3 (2013):397-406,
https://doi.org/10.1007/s11240-012-0279-6 . .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Mitić, Nevena
AU  - Adžić, Slađan
AU  - Pavlović, Nenad
AU  - Zdravković, Milan
AU  - Vinterhalter, Dragan
PY  - 2010
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/86
AB  - Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse.
AB  - Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower
T1  - Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola
EP  - 345
IS  - 2
SP  - 337
VL  - 62
DO  - 10.2298/ABS1002337P
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Mitić, Nevena and Adžić, Slađan and Pavlović, Nenad and Zdravković, Milan and Vinterhalter, Dragan",
year = "2010",
abstract = "Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse., Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower, Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola",
pages = "345-337",
number = "2",
volume = "62",
doi = "10.2298/ABS1002337P"
}

Pavlović, S., Vinterhalter, B., Mitić, N., Adžić, S., Pavlović, N., Zdravković, M.,& Vinterhalter, D.. (2010). In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 337-345.
https://doi.org/10.2298/ABS1002337P

Pavlović S, Vinterhalter B, Mitić N, Adžić S, Pavlović N, Zdravković M, Vinterhalter D. In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower. in Archives of Biological Sciences. 2010;62(2):337-345.
doi:10.2298/ABS1002337P .

Pavlović, Suzana, Vinterhalter, Branka, Mitić, Nevena, Adžić, Slađan, Pavlović, Nenad, Zdravković, Milan, Vinterhalter, Dragan, "In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower" in Archives of Biological Sciences, 62, no. 2 (2010):337-345,
https://doi.org/10.2298/ABS1002337P . .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana
AU  - Ninković, S.
AU  - Miljuš-Đukić, J
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
PY  - 2006
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/57
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata
EP  - 530
IS  - 4
SP  - 525
VL  - 50
DO  - 10.1007/s10535-006-0083-4
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana and Ninković, S. and Miljuš-Đukić, J and Vinterhalter, Branka and Vinterhalter, Dragan",
year = "2006",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata",
pages = "530-525",
number = "4",
volume = "50",
doi = "10.1007/s10535-006-0083-4"
}

Sretenović-Rajičić, T., Ninković, S., Miljuš-Đukić, J., Vinterhalter, B.,& Vinterhalter, D.. (2006). Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 50(4), 525-530.
https://doi.org/10.1007/s10535-006-0083-4

Sretenović-Rajičić T, Ninković S, Miljuš-Đukić J, Vinterhalter B, Vinterhalter D. Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum. 2006;50(4):525-530.
doi:10.1007/s10535-006-0083-4 .

Sretenović-Rajičić, Tatjana, Ninković, S., Miljuš-Đukić, J, Vinterhalter, Branka, Vinterhalter, Dragan, "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata" in Biologia Plantarum, 50, no. 4 (2006):525-530,
https://doi.org/10.1007/s10535-006-0083-4 . .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana D.
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
PY  - 2006
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/221
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.
T2  - Biologia Plantarum
T1  - Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata
EP  - 530
IS  - 4
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_rivec_221
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana D. and Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Vinterhalter, Branka and Vinterhalter, Dragan V",
year = "2006",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.",
journal = "Biologia Plantarum",
title = "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata",
pages = "530",
number = "4",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_rivec_221"
}

Sretenović-Rajičić, T. D., Ninković, S., Miljuš-Đukić, J. D., Vinterhalter, B.,& Vinterhalter, D. V.. (2006). Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_rivec_221

Sretenović-Rajičić TD, Ninković S, Miljuš-Đukić JD, Vinterhalter B, Vinterhalter DV. Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum. 2006;50(4):null-530.
https://hdl.handle.net/21.15107/rcub_rivec_221 .

Sretenović-Rajičić, Tatjana D., Ninković, Slavica, Miljuš-Đukić, Jovanka D., Vinterhalter, Branka, Vinterhalter, Dragan V, "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_rivec_221 .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana
AU  - Ninković, S.
AU  - Vinterhalter, Branka
AU  - Miljuš-Đukić, J
AU  - Vinterhalter, Dragan
PY  - 2004
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/55
AB  - Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Introduction of resistance to herbicide Basta (R) in Savoy cabbage
EP  - 436
IS  - 3
SP  - 431
VL  - 48
DO  - 10.1023/B:BIOP.0000041098.18836.be
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana and Ninković, S. and Vinterhalter, Branka and Miljuš-Đukić, J and Vinterhalter, Dragan",
year = "2004",
abstract = "Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Introduction of resistance to herbicide Basta (R) in Savoy cabbage",
pages = "436-431",
number = "3",
volume = "48",
doi = "10.1023/B:BIOP.0000041098.18836.be"
}

Sretenović-Rajičić, T., Ninković, S., Vinterhalter, B., Miljuš-Đukić, J.,& Vinterhalter, D.. (2004). Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 48(3), 431-436.
https://doi.org/10.1023/B:BIOP.0000041098.18836.be

Sretenović-Rajičić T, Ninković S, Vinterhalter B, Miljuš-Đukić J, Vinterhalter D. Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum. 2004;48(3):431-436.
doi:10.1023/B:BIOP.0000041098.18836.be .

Sretenović-Rajičić, Tatjana, Ninković, S., Vinterhalter, Branka, Miljuš-Đukić, J, Vinterhalter, Dragan, "Introduction of resistance to herbicide Basta (R) in Savoy cabbage" in Biologia Plantarum, 48, no. 3 (2004):431-436,
https://doi.org/10.1023/B:BIOP.0000041098.18836.be . .

TY  - CONF
AU  - Sretenović-Rajičić, Tatjana
AU  - Mijatović, Mirjana
AU  - Vinterhalter, Dragan
PY  - 2002
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/40
AB  - Cabbage (Brassica oleracea var. capitata) is one of the most common vegetables in Yugoslavia. Our breeding programs on Brassicas are developed with the aim to create genetically pure lines which could be used for creation of various hybrids and new cultivars. Since there are barriers which prevent fast realization of these goals, we have introduced in vitro culture techniques in our breeding programs. Thus we investigated possibilities of using micropropagation, anther culture, in vitro selection and genetic engineering methods as tools to improve our Brassicas and create new perspective genotypes suitable for uses in conventional breeding methods. So far we have clonned a number of perspective Brassica genotypes and identified the most appropriate medium for their growth and multiplication. We present here our efforts to overcome incompatibility barriers by attempting to create double-haploid genotypes of cabbage through anther culture. Identifying possible tolerance of our domestic populations to herbicide by in vitro selection is also one of our goals. We have also created our first transformed Brassicas, performing Agrobacterium-mediated transformation. In this paper we have presented our research in these areas.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes
T1  - In vitro culture as a tool for improvement of cabbage cultivars in Yugoslavia
EP  - 213
IS  - 579
SP  - 209
DO  - 10.17660/ActaHortic.2002.579.34
ER  - 

@conference{
author = "Sretenović-Rajičić, Tatjana and Mijatović, Mirjana and Vinterhalter, Dragan",
year = "2002",
abstract = "Cabbage (Brassica oleracea var. capitata) is one of the most common vegetables in Yugoslavia. Our breeding programs on Brassicas are developed with the aim to create genetically pure lines which could be used for creation of various hybrids and new cultivars. Since there are barriers which prevent fast realization of these goals, we have introduced in vitro culture techniques in our breeding programs. Thus we investigated possibilities of using micropropagation, anther culture, in vitro selection and genetic engineering methods as tools to improve our Brassicas and create new perspective genotypes suitable for uses in conventional breeding methods. So far we have clonned a number of perspective Brassica genotypes and identified the most appropriate medium for their growth and multiplication. We present here our efforts to overcome incompatibility barriers by attempting to create double-haploid genotypes of cabbage through anther culture. Identifying possible tolerance of our domestic populations to herbicide by in vitro selection is also one of our goals. We have also created our first transformed Brassicas, performing Agrobacterium-mediated transformation. In this paper we have presented our research in these areas.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes",
title = "In vitro culture as a tool for improvement of cabbage cultivars in Yugoslavia",
pages = "213-209",
number = "579",
doi = "10.17660/ActaHortic.2002.579.34"
}

Sretenović-Rajičić, T., Mijatović, M.,& Vinterhalter, D.. (2002). In vitro culture as a tool for improvement of cabbage cultivars in Yugoslavia. in Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes
International Society for Horticultural Science.(579), 209-213.
https://doi.org/10.17660/ActaHortic.2002.579.34

Sretenović-Rajičić T, Mijatović M, Vinterhalter D. In vitro culture as a tool for improvement of cabbage cultivars in Yugoslavia. in Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes. 2002;(579):209-213.
doi:10.17660/ActaHortic.2002.579.34 .

Sretenović-Rajičić, Tatjana, Mijatović, Mirjana, Vinterhalter, Dragan, "In vitro culture as a tool for improvement of cabbage cultivars in Yugoslavia" in Acta Horticulturae : Proceedings of the Second Balkan Symposium on Vegetables and Potatoes, no. 579 (2002):209-213,
https://doi.org/10.17660/ActaHortic.2002.579.34 . .

TY  - CONF
AU  - Sretenović-Rajičić, Tatjana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 1997
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/21
AB  - Considering the great toxicity paraquat has to a wide range of plant and animal species, including man, we have investigated the possible use of cabbage in vitro cultures as a model system suitable to study this pesticide. The main aim of this study was to investigate the effect of paraquat on growth and development of cabbage shoot cultures. Special attention was paid to the detection of eventual means by which cultures can overcome the detrimental effect of the chemical, and also to investigate if some of the genotypes coming from the breeding program have an increased tolerance to paraquat. Promising domestic genotypes PI94, P295, P6p, P44 were investigated in the rooting stage. A root induction medium containing MS inorganic salts and 5.0 mgl-1 IB A was also supplemented with Paraquat at 0.5, 2.0, 10.0, 50.0 mgl -1. Shoots were treated for 24 hours and then transferred to a hormone free medium. After 28 days we measured shoot length, number of internodes, percentage of rooting, root length and formation of laterals. Paraquat decreased all the parameters which were followed. On leaves it induced lesions. Genotypes P44 and P6p were more tolerant to paraquat then the other two genotypes. Genotype P44 was interesting since in some plants lateral shoot formation occurred.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro
EP  - 593
SP  - 589
VL  - 462
DO  - 10.17660/actahortic.1997.462.87
ER  - 

@conference{
author = "Sretenović-Rajičić, Tatjana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "1997",
abstract = "Considering the great toxicity paraquat has to a wide range of plant and animal species, including man, we have investigated the possible use of cabbage in vitro cultures as a model system suitable to study this pesticide. The main aim of this study was to investigate the effect of paraquat on growth and development of cabbage shoot cultures. Special attention was paid to the detection of eventual means by which cultures can overcome the detrimental effect of the chemical, and also to investigate if some of the genotypes coming from the breeding program have an increased tolerance to paraquat. Promising domestic genotypes PI94, P295, P6p, P44 were investigated in the rooting stage. A root induction medium containing MS inorganic salts and 5.0 mgl-1 IB A was also supplemented with Paraquat at 0.5, 2.0, 10.0, 50.0 mgl -1. Shoots were treated for 24 hours and then transferred to a hormone free medium. After 28 days we measured shoot length, number of internodes, percentage of rooting, root length and formation of laterals. Paraquat decreased all the parameters which were followed. On leaves it induced lesions. Genotypes P44 and P6p were more tolerant to paraquat then the other two genotypes. Genotype P44 was interesting since in some plants lateral shoot formation occurred.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro",
pages = "593-589",
volume = "462",
doi = "10.17660/actahortic.1997.462.87"
}

Sretenović-Rajičić, T., Vinterhalter, D.,& Vinterhalter, B.. (1997). The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro. in Acta Horticulturae
International Society for Horticultural Science., 462, 589-593.
https://doi.org/10.17660/actahortic.1997.462.87

Sretenović-Rajičić T, Vinterhalter D, Vinterhalter B. The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro. in Acta Horticulturae. 1997;462:589-593.
doi:10.17660/actahortic.1997.462.87 .

Sretenović-Rajičić, Tatjana, Vinterhalter, Dragan, Vinterhalter, Branka, "The effects of paraquat on cabbage (Brassica oleracea var. capitata L.) cultured in vitro" in Acta Horticulturae, 462 (1997):589-593,
https://doi.org/10.17660/actahortic.1997.462.87 . .