TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana
AU  - Ninković, S.
AU  - Miljuš-Đukić, J
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
PY  - 2006
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/57
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata
EP  - 530
IS  - 4
SP  - 525
VL  - 50
DO  - 10.1007/s10535-006-0083-4
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana and Ninković, S. and Miljuš-Đukić, J and Vinterhalter, Branka and Vinterhalter, Dragan",
year = "2006",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata",
pages = "530-525",
number = "4",
volume = "50",
doi = "10.1007/s10535-006-0083-4"
}

Sretenović-Rajičić, T., Ninković, S., Miljuš-Đukić, J., Vinterhalter, B.,& Vinterhalter, D.. (2006). Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 50(4), 525-530.
https://doi.org/10.1007/s10535-006-0083-4

Sretenović-Rajičić T, Ninković S, Miljuš-Đukić J, Vinterhalter B, Vinterhalter D. Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum. 2006;50(4):525-530.
doi:10.1007/s10535-006-0083-4 .

Sretenović-Rajičić, Tatjana, Ninković, S., Miljuš-Đukić, J, Vinterhalter, Branka, Vinterhalter, Dragan, "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata" in Biologia Plantarum, 50, no. 4 (2006):525-530,
https://doi.org/10.1007/s10535-006-0083-4 . .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana
AU  - Ninković, S.
AU  - Vinterhalter, Branka
AU  - Miljuš-Đukić, J
AU  - Vinterhalter, Dragan
PY  - 2004
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/55
AB  - Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Introduction of resistance to herbicide Basta (R) in Savoy cabbage
EP  - 436
IS  - 3
SP  - 431
VL  - 48
DO  - 10.1023/B:BIOP.0000041098.18836.be
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana and Ninković, S. and Vinterhalter, Branka and Miljuš-Đukić, J and Vinterhalter, Dragan",
year = "2004",
abstract = "Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Introduction of resistance to herbicide Basta (R) in Savoy cabbage",
pages = "436-431",
number = "3",
volume = "48",
doi = "10.1023/B:BIOP.0000041098.18836.be"
}

Sretenović-Rajičić, T., Ninković, S., Vinterhalter, B., Miljuš-Đukić, J.,& Vinterhalter, D.. (2004). Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 48(3), 431-436.
https://doi.org/10.1023/B:BIOP.0000041098.18836.be

Sretenović-Rajičić T, Ninković S, Vinterhalter B, Miljuš-Đukić J, Vinterhalter D. Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum. 2004;48(3):431-436.
doi:10.1023/B:BIOP.0000041098.18836.be .

Sretenović-Rajičić, Tatjana, Ninković, S., Vinterhalter, Branka, Miljuš-Đukić, J, Vinterhalter, Dragan, "Introduction of resistance to herbicide Basta (R) in Savoy cabbage" in Biologia Plantarum, 48, no. 3 (2004):431-436,
https://doi.org/10.1023/B:BIOP.0000041098.18836.be . .