Zdravković-Korać, Snežana

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  • Zdravković-Korać, Snežana (5)
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Author's Bibliography

Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)

Belić, Maja; Zdravković-Korać, Snežana; Pavlović, Suzana; Živković, Suzana; Vinterhalter, Dragan; Ćalić, Dušica; Milojević, Jelena

(East Sarajevo : Faculty of Agriculture, 2021)

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/465
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
C3  - Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
EP  - 213
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_rivec_465
ER  - 
@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213-213",
url = "https://hdl.handle.net/21.15107/rcub_rivec_465"
}
Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
East Sarajevo : Faculty of Agriculture., 213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465
Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10. 2021;:213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465 .
Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 (2021):213-213,
https://hdl.handle.net/21.15107/rcub_rivec_465 .

Variability in somatic embryo-forming capacity of spinach

Belić, Maja; Zdravković-Korać, Snežana; Uzelac, Branka; Calić, Dušica; Pavlović, Suzana; Milojević, Jelena

(Nature Research, Berlin, 2020)

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Calić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/214
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80-100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20-60% of regenerating explants. The explants from the majority of seedlings (52-100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10-20 (43.5%) up to>20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1-10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research, Berlin
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
ER  - 
@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Calić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80-100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20-60% of regenerating explants. The explants from the majority of seedlings (52-100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10-20 (43.5%) up to>20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1-10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research, Berlin",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9"
}
Belić, M., Zdravković-Korać, S., Uzelac, B., Calić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research, Berlin., 10(1).
https://doi.org/10.1038/s41598-020-76279-9
Belić M, Zdravković-Korać S, Uzelac B, Calić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1).
doi:10.1038/s41598-020-76279-9 .
Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Calić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020),
https://doi.org/10.1038/s41598-020-76279-9 . .
5
4

Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)

Pavlović, Suzana; Vinterhalter, Branka; Zdravković-Korać, Snežana; Vinterhalter, Dragan; Zdravković, Jasmina; Cvikić, Dejan; Mitić, Nevena

(Springer, Dordrecht, 2013)

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/143
AB  - A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)
EP  - 406
IS  - 3
SP  - 397
VL  - 113
DO  - 10.1007/s11240-012-0279-6
ER  - 
@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Vinterhalter, Dragan and Zdravković, Jasmina and Cvikić, Dejan and Mitić, Nevena",
year = "2013",
abstract = "A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)",
pages = "406-397",
number = "3",
volume = "113",
doi = "10.1007/s11240-012-0279-6"
}
Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Vinterhalter, D., Zdravković, J., Cvikić, D.,& Mitić, N.. (2013). Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 113(3), 397-406.
https://doi.org/10.1007/s11240-012-0279-6
Pavlović S, Vinterhalter B, Zdravković-Korać S, Vinterhalter D, Zdravković J, Cvikić D, Mitić N. Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture. 2013;113(3):397-406.
doi:10.1007/s11240-012-0279-6 .
Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Vinterhalter, Dragan, Zdravković, Jasmina, Cvikić, Dejan, Mitić, Nevena, "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)" in Plant Cell Tissue and Organ Culture, 113, no. 3 (2013):397-406,
https://doi.org/10.1007/s11240-012-0279-6 . .
28
18
27

Long days promote somatic embryogenesis in spinach

Milojević, Jelena; Tubić, Ljiljana; Pavlović, Suzana; Mitić, Nevena; Calić, Dušica; Vinterhalter, Branka; Zdravković-Korać, Snežana

(Elsevier Science Bv, Amsterdam, 2012)

TY  - JOUR
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Pavlović, Suzana
AU  - Mitić, Nevena
AU  - Calić, Dušica
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/123
AB  - As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Long days promote somatic embryogenesis in spinach
EP  - 37
SP  - 32
VL  - 142
DO  - 10.1016/j.scienta.2012.04.020
ER  - 
@article{
author = "Milojević, Jelena and Tubić, Ljiljana and Pavlović, Suzana and Mitić, Nevena and Calić, Dušica and Vinterhalter, Branka and Zdravković-Korać, Snežana",
year = "2012",
abstract = "As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Long days promote somatic embryogenesis in spinach",
pages = "37-32",
volume = "142",
doi = "10.1016/j.scienta.2012.04.020"
}
Milojević, J., Tubić, L., Pavlović, S., Mitić, N., Calić, D., Vinterhalter, B.,& Zdravković-Korać, S.. (2012). Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 142, 32-37.
https://doi.org/10.1016/j.scienta.2012.04.020
Milojević J, Tubić L, Pavlović S, Mitić N, Calić D, Vinterhalter B, Zdravković-Korać S. Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae. 2012;142:32-37.
doi:10.1016/j.scienta.2012.04.020 .
Milojević, Jelena, Tubić, Ljiljana, Pavlović, Suzana, Mitić, Nevena, Calić, Dušica, Vinterhalter, Branka, Zdravković-Korać, Snežana, "Long days promote somatic embryogenesis in spinach" in Scientia Horticulturae, 142 (2012):32-37,
https://doi.org/10.1016/j.scienta.2012.04.020 . .
6
5
7

Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance

Pavlović, Suzana; Vinterhalter, Branka; Zdravković-Korać, Snežana; Adžić, Slađan; Đorđević, Mladen; Ugrinović, Milan; Mitić, Nevena

(Beograd : Serbian Plant Physiology Society, 2011)

TY  - CONF
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Adžić, Slađan
AU  - Đorđević, Mladen
AU  - Ugrinović, Milan
AU  - Mitić, Nevena
PY  - 2011
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/577
AB  - Cauliflower (Brassica oleracea var. botrytis) is an important vegetable plant grown worldwide that is impacted by biotic and abiotic stresses. Plant biotechnology could be an efficient approach to enhance stress tolerance. Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as biotic and abiotic stress response. Thus, we have established an Agrobaterium tumefacens-mediated protocol for transformation of cauliflower (line KFRM4) using the binary vector pSHX004 with the mitogen-activated protein kinase kinase kinase (MAPKKK) gene from tobacco (NPK1) for stress tolerance and the phosphinothricin acetyltransferase (bar) selectable marker gene for herbicide resistance. The effect of acetosyringone (AS) on transformation efficiency was also investigated. Hypocotyls of 8 day-old seedlings, pre-cultivated on MS shoot induction medium (SIM) supplemented with 1.0 mg/l BA and 0.2 mg/l IBA for 3 days, were infected with bacterial suspension containing 0 or 100 μM AS and than co-cultivated on SIM with the same concentration of AS as infection medium for 3 days. Upon co-cultivation, the explants were cultured on SIM containing  500 mg/l cefotaxime for 8 days, and than subcultured on SIM selection media with 5 mg/l phosphinothricin (PPT) until shoot regeneration. The addition of AS during co-cultivation increased transformation frequency (7.8% vs. 1.4% in its absence). Selected PPT-resistant shoots were continuously maintained and multiplied on MS selection medium containing 2 mg/l PPT and BA or Kin (0.5-1.0 mg/l) alone or in combination with IBA (0.1-0.2 mg/l) displaying vigorous growth and development. Integration of the bar gene in transgenic shoots was verified by chlorophenol red (CR) assay while PCR confirmed the presence of NPK1 gene. Transformed plants provide a promising material for stress tolerance study.
PB  - Beograd : Serbian Plant Physiology Society
PB  - Beograd : Institute for Biological Research "Siniša Stanković"
C3  - 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts
T1  - Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_rivec_577
ER  - 
@conference{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Adžić, Slađan and Đorđević, Mladen and Ugrinović, Milan and Mitić, Nevena",
year = "2011",
abstract = "Cauliflower (Brassica oleracea var. botrytis) is an important vegetable plant grown worldwide that is impacted by biotic and abiotic stresses. Plant biotechnology could be an efficient approach to enhance stress tolerance. Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as biotic and abiotic stress response. Thus, we have established an Agrobaterium tumefacens-mediated protocol for transformation of cauliflower (line KFRM4) using the binary vector pSHX004 with the mitogen-activated protein kinase kinase kinase (MAPKKK) gene from tobacco (NPK1) for stress tolerance and the phosphinothricin acetyltransferase (bar) selectable marker gene for herbicide resistance. The effect of acetosyringone (AS) on transformation efficiency was also investigated. Hypocotyls of 8 day-old seedlings, pre-cultivated on MS shoot induction medium (SIM) supplemented with 1.0 mg/l BA and 0.2 mg/l IBA for 3 days, were infected with bacterial suspension containing 0 or 100 μM AS and than co-cultivated on SIM with the same concentration of AS as infection medium for 3 days. Upon co-cultivation, the explants were cultured on SIM containing  500 mg/l cefotaxime for 8 days, and than subcultured on SIM selection media with 5 mg/l phosphinothricin (PPT) until shoot regeneration. The addition of AS during co-cultivation increased transformation frequency (7.8% vs. 1.4% in its absence). Selected PPT-resistant shoots were continuously maintained and multiplied on MS selection medium containing 2 mg/l PPT and BA or Kin (0.5-1.0 mg/l) alone or in combination with IBA (0.1-0.2 mg/l) displaying vigorous growth and development. Integration of the bar gene in transgenic shoots was verified by chlorophenol red (CR) assay while PCR confirmed the presence of NPK1 gene. Transformed plants provide a promising material for stress tolerance study.",
publisher = "Beograd : Serbian Plant Physiology Society, Beograd : Institute for Biological Research "Siniša Stanković"",
journal = "1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts",
title = "Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_rivec_577"
}
Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Adžić, S., Đorđević, M., Ugrinović, M.,& Mitić, N.. (2011). Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance. in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts
Beograd : Serbian Plant Physiology Society., 64.
https://hdl.handle.net/21.15107/rcub_rivec_577
Pavlović S, Vinterhalter B, Zdravković-Korać S, Adžić S, Đorđević M, Ugrinović M, Mitić N. Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance. in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts. 2011;:64.
https://hdl.handle.net/21.15107/rcub_rivec_577 .
Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Adžić, Slađan, Đorđević, Mladen, Ugrinović, Milan, Mitić, Nevena, "Agrobacterium-mediated genetic transformation of cauliflower with the NPK1 gene to enhance biotic and abiotic stress tolerance" in 1st International conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society : Programme and Abstracts (2011):64,
https://hdl.handle.net/21.15107/rcub_rivec_577 .