Milojević, Jelena

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Authority KeyName Variants
orcid::0000-0001-8822-9771
  • Milojević, Jelena (8)
  • Милојевић, Јелена (1)

Author's Bibliography

Assessment of eggplant (Solanum melongena L.) 1 genotypes and selection of parameters for better yield

Damnjanović, Jelena; Girek, Zdenka; Milojević, Jelena; Zečević, Veselinka; Živanović, Tomislav; Ugrinović, Milan; Pavlović, Suzana

(MDPI, 2022)

TY  - CONF
AU  - Damnjanović, Jelena
AU  - Girek, Zdenka
AU  - Milojević, Jelena
AU  - Zečević, Veselinka
AU  - Živanović, Tomislav
AU  - Ugrinović, Milan
AU  - Pavlović, Suzana
PY  - 2022
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/491
AB  - One of the goals in eggplant breeding (Solanum melongena L.) is higher yield. The research included 20 different genotypes that are part of the collection of the Institute for Vegetable Crops Smederevska Palanka. 16 genotypes originated from Serbia, 2 from the Netherlands, 1 from Italy and 1 from Israel. The experiment was conducted at locations Vranovo (44°36’6,35”N, 20°59’55.47”E, altitude 87 m) using a randomized complete block design in three replications. Yield parameters (earliness, plant height/cm, number of fruits per plant, fruit weight/g, fruit length and width/cm, fruit yield per genotype/kg) were examined to study the traits relations and to effects on eggplant yield. Number of fruits per plant had positive and significant correlation with yield (r=0.685**). Also results showed positive correlation between each of: plant height to fruit length (r=0.812**), plant height to fruit weight (r=0.147), fruit width to fruit weight (r=0.523*). This characteristics had direct or indirect positive effect on yield so it can be taken as selection criteria to increase final yield of eggplant. Because there is genetic variability between tested genotypes, progress in breeding will depends on this variation. Earliness had negative correlation with yield (r=-0.044) and with all others parameters.
PB  - MDPI
C3  - Chemistry Proceedings
T1  - Assessment of eggplant (Solanum melongena L.) 1 genotypes and selection of parameters for better yield
IS  - 10
SP  - 31
DO  - 10.3390/IOCAG2022-12309
ER  - 
@conference{
author = "Damnjanović, Jelena and Girek, Zdenka and Milojević, Jelena and Zečević, Veselinka and Živanović, Tomislav and Ugrinović, Milan and Pavlović, Suzana",
year = "2022",
abstract = "One of the goals in eggplant breeding (Solanum melongena L.) is higher yield. The research included 20 different genotypes that are part of the collection of the Institute for Vegetable Crops Smederevska Palanka. 16 genotypes originated from Serbia, 2 from the Netherlands, 1 from Italy and 1 from Israel. The experiment was conducted at locations Vranovo (44°36’6,35”N, 20°59’55.47”E, altitude 87 m) using a randomized complete block design in three replications. Yield parameters (earliness, plant height/cm, number of fruits per plant, fruit weight/g, fruit length and width/cm, fruit yield per genotype/kg) were examined to study the traits relations and to effects on eggplant yield. Number of fruits per plant had positive and significant correlation with yield (r=0.685**). Also results showed positive correlation between each of: plant height to fruit length (r=0.812**), plant height to fruit weight (r=0.147), fruit width to fruit weight (r=0.523*). This characteristics had direct or indirect positive effect on yield so it can be taken as selection criteria to increase final yield of eggplant. Because there is genetic variability between tested genotypes, progress in breeding will depends on this variation. Earliness had negative correlation with yield (r=-0.044) and with all others parameters.",
publisher = "MDPI",
journal = "Chemistry Proceedings",
title = "Assessment of eggplant (Solanum melongena L.) 1 genotypes and selection of parameters for better yield",
number = "10",
pages = "31",
doi = "10.3390/IOCAG2022-12309"
}
Damnjanović, J., Girek, Z., Milojević, J., Zečević, V., Živanović, T., Ugrinović, M.,& Pavlović, S.. (2022). Assessment of eggplant (Solanum melongena L.) 1 genotypes and selection of parameters for better yield. in Chemistry Proceedings
MDPI.(10), 31.
https://doi.org/10.3390/IOCAG2022-12309
Damnjanović J, Girek Z, Milojević J, Zečević V, Živanović T, Ugrinović M, Pavlović S. Assessment of eggplant (Solanum melongena L.) 1 genotypes and selection of parameters for better yield. in Chemistry Proceedings. 2022;(10):31.
doi:10.3390/IOCAG2022-12309 .
Damnjanović, Jelena, Girek, Zdenka, Milojević, Jelena, Zečević, Veselinka, Živanović, Tomislav, Ugrinović, Milan, Pavlović, Suzana, "Assessment of eggplant (Solanum melongena L.) 1 genotypes and selection of parameters for better yield" in Chemistry Proceedings, no. 10 (2022):31,
https://doi.org/10.3390/IOCAG2022-12309 . .
2

Influence of genotypes and environment on eggplant fruit length and width

Damnjanović, Jelena; Pavlović, Suzana; Girek, Zdenka; Milojević, Jelena; Adžić, Slađan; Živanović, Tomislav; Ugrinović, Milan

(Istočno Sarajevo : Poljoprivredni fakultet, 2021)

TY  - CONF
AU  - Damnjanović, Jelena
AU  - Pavlović, Suzana
AU  - Girek, Zdenka
AU  - Milojević, Jelena
AU  - Adžić, Slađan
AU  - Živanović, Tomislav
AU  - Ugrinović, Milan
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/409
AB  - One of the goals in eggplant breeding (Solanum melongena L.) is higher yield, as well as
adaptation to different environments. Our study included 20 different eggplant genotypes. The
experiment was conducted at three different locations (Smederevska Palanka, Vranovo and
Kusadak) using a randomized complete block design in three replications. The length and width
of fruit were examined, characteristics important both for the shape and the weight of the fruit.
Two-factors ANOVA determined a statistically significant influence of genotype and locality for
fruit length and width. Average fruit lengths ranged from 134.86 mm (K19) to 246.07 mm
(K38). The mean value of fruit length from the Kusadak locality was significantly (p<0.01)
higher than the general average (181.58 mm), and significantly longer fruit length was recorded
in 10 genotypes: K6, K13, K15, K16, K21, K22, K22, K25, K36 and K39. The highest average
fruit width (120.05 mm) was recorded in genotype K19 and differed significantly in relation to
the average values of other observed genotypes. The lowest average fruit width was recorded in
the K38 genotype at Smederevska Palanka and Kusadak localities (54.33 mm and 54.82 mm).
According to AMMI analysis, the least stable locality in terms of fruit length was Kusadak,
while the most stable was Smederevska Palanka with an AMMI stability coefficient of 7.00. The
most stable genotype was K3 (0.76). The locality Smederevska Palanka was the most stable for
fruit width (9.50), while the most stable genotype was K7 (0.28). The least stable locality was
Vranovo.
PB  - Istočno Sarajevo : Poljoprivredni fakultet
C3  - Book of Proceedings XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
T1  - Influence of genotypes and environment on eggplant fruit length and width
EP  - 187
SP  - 181
UR  - https://hdl.handle.net/21.15107/rcub_rivec_409
ER  - 
@conference{
author = "Damnjanović, Jelena and Pavlović, Suzana and Girek, Zdenka and Milojević, Jelena and Adžić, Slađan and Živanović, Tomislav and Ugrinović, Milan",
year = "2021",
abstract = "One of the goals in eggplant breeding (Solanum melongena L.) is higher yield, as well as
adaptation to different environments. Our study included 20 different eggplant genotypes. The
experiment was conducted at three different locations (Smederevska Palanka, Vranovo and
Kusadak) using a randomized complete block design in three replications. The length and width
of fruit were examined, characteristics important both for the shape and the weight of the fruit.
Two-factors ANOVA determined a statistically significant influence of genotype and locality for
fruit length and width. Average fruit lengths ranged from 134.86 mm (K19) to 246.07 mm
(K38). The mean value of fruit length from the Kusadak locality was significantly (p<0.01)
higher than the general average (181.58 mm), and significantly longer fruit length was recorded
in 10 genotypes: K6, K13, K15, K16, K21, K22, K22, K25, K36 and K39. The highest average
fruit width (120.05 mm) was recorded in genotype K19 and differed significantly in relation to
the average values of other observed genotypes. The lowest average fruit width was recorded in
the K38 genotype at Smederevska Palanka and Kusadak localities (54.33 mm and 54.82 mm).
According to AMMI analysis, the least stable locality in terms of fruit length was Kusadak,
while the most stable was Smederevska Palanka with an AMMI stability coefficient of 7.00. The
most stable genotype was K3 (0.76). The locality Smederevska Palanka was the most stable for
fruit width (9.50), while the most stable genotype was K7 (0.28). The least stable locality was
Vranovo.",
publisher = "Istočno Sarajevo : Poljoprivredni fakultet",
journal = "Book of Proceedings XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10",
title = "Influence of genotypes and environment on eggplant fruit length and width",
pages = "187-181",
url = "https://hdl.handle.net/21.15107/rcub_rivec_409"
}
Damnjanović, J., Pavlović, S., Girek, Z., Milojević, J., Adžić, S., Živanović, T.,& Ugrinović, M.. (2021). Influence of genotypes and environment on eggplant fruit length and width. in Book of Proceedings XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
Istočno Sarajevo : Poljoprivredni fakultet., 181-187.
https://hdl.handle.net/21.15107/rcub_rivec_409
Damnjanović J, Pavlović S, Girek Z, Milojević J, Adžić S, Živanović T, Ugrinović M. Influence of genotypes and environment on eggplant fruit length and width. in Book of Proceedings XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10. 2021;:181-187.
https://hdl.handle.net/21.15107/rcub_rivec_409 .
Damnjanović, Jelena, Pavlović, Suzana, Girek, Zdenka, Milojević, Jelena, Adžić, Slađan, Živanović, Tomislav, Ugrinović, Milan, "Influence of genotypes and environment on eggplant fruit length and width" in Book of Proceedings XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 (2021):181-187,
https://hdl.handle.net/21.15107/rcub_rivec_409 .

Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)

Belić, Maja; Zdravković-Korać, Snežana; Pavlović, Suzana; Živković, Suzana; Vinterhalter, Dragan; Ćalić, Dušica; Milojević, Jelena

(East Sarajevo : Faculty of Agriculture, 2021)

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/465
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
C3  - Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
EP  - 213
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_rivec_465
ER  - 
@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213-213",
url = "https://hdl.handle.net/21.15107/rcub_rivec_465"
}
Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10
East Sarajevo : Faculty of Agriculture., 213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465
Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10. 2021;:213-213.
https://hdl.handle.net/21.15107/rcub_rivec_465 .
Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts XII International Scientific Agriculture Symposium "AGROSYM 2021", Jahorina, October 07 - 10 (2021):213-213,
https://hdl.handle.net/21.15107/rcub_rivec_465 .

Испитивање ефекта осмотског стреса код карфиола in vitro

Павловић, Сузана; Дамњановић, Јелена; Гирек, Зденка; Угриновић, Милан; Аџић, Слађан; Милојевић, Јелена; Брдар-Јокановић, Милка

(Смедеревска Паланка : Институт за повртарство, 2021)

TY  - CONF
AU  - Павловић, Сузана
AU  - Дамњановић, Јелена
AU  - Гирек, Зденка
AU  - Угриновић, Милан
AU  - Аџић, Слађан
AU  - Милојевић, Јелена
AU  - Брдар-Јокановић, Милка
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/429
AB  - Циљ истраживања је био испитивање ефекта осмотског стреса
изазваног стресом соли и суше на растење и садржај суве материје
код линија карфиола гајених in vitro. Две одабране линије карфиола
су изложене in vitro стресу соли и суше гајењем на МS подлози са 0,
100, 200, 300 и 400 mМ NaCl односно гајењем на МS подлози са 0, 5,
10, 15 и 20% PEG. Свежа маса биљке и садржај суве материје су
мерени на почетку и након 8 дана третмана. Оба стреса су довела до
редукције растења и повећања садржаја суве материје, али су линије
реаговале различито на појединачне стресоре и њихове
концентрације. Линија Кф-Л1 је боље реаговала на ниже
концентрације NaCl, на подлози са 300 мМ NaCl обе линије су
показивале сличан пораст, али на вишој концентрацији од 400 мМ
линија Кф-Л1 је показала значајно мању толеранцију на повишени
салинитет у односу на линију Кф-Л2. Добијени резултати указују на
разлике међу тестираним линијама у толеранцији према стресу соли
и суше у in vitro условима, што ову методу чини ефикасном у
тестирању и селекцији толерантнијих линија карфиола и других
купусњача на осмотски стрес.
AB  - The aim of the study was to examine the effect of osmotic stress (salt
and drought stress) on growth and dry matter content in cauliflower lines
grown in vitro. Two selected cauliflower lines were exposed in vitro to
salt stress and drought by culturing on MS medium with 0, 100, 200, 300
and 400 mM NaCl and resp. by culturing on MS medium with 0, 5, 10,
15 and 20% PEG. The fresh weight and the dry matter content were
measured at the beginning and after 8 days of treatment. Both stresses led
to a reduction in the growth and increase in dry matter content, but the
lines reacted differently to individual stressors and their concentrations.
The Kf-L1 line responded better to lower NaCl concentrations, on a
medium with 300 mM NaCl both lines showed a similar increase, but at a
higher concentration of 400 mM the Kf-L1 line showed significantly
lower tolerance to increased salinity compared to the Kf-L2line. Obtained
resultsindicate differences between the tested lines in tolerance to salt and
drought stress, which makes this method effective in testing and selecting
more tolerant lines of cauliflower and Brassica vegetables.
PB  - Смедеревска Паланка : Институт за повртарство
C3  - Биотехнологија и савремени приступ у гајењу и оплемењивању биља, научно-стручни скуп са међународним учешћем, Зборник радова, Смедеревска Паланка, 15. децембар
T1  - Испитивање ефекта осмотског стреса код карфиола in vitro
T1  - Examination of the effect of osmotic stress in caulifiols in vitro
EP  - 144
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_rivec_429
ER  - 
@conference{
author = "Павловић, Сузана and Дамњановић, Јелена and Гирек, Зденка and Угриновић, Милан and Аџић, Слађан and Милојевић, Јелена and Брдар-Јокановић, Милка",
year = "2021",
abstract = "Циљ истраживања је био испитивање ефекта осмотског стреса
изазваног стресом соли и суше на растење и садржај суве материје
код линија карфиола гајених in vitro. Две одабране линије карфиола
су изложене in vitro стресу соли и суше гајењем на МS подлози са 0,
100, 200, 300 и 400 mМ NaCl односно гајењем на МS подлози са 0, 5,
10, 15 и 20% PEG. Свежа маса биљке и садржај суве материје су
мерени на почетку и након 8 дана третмана. Оба стреса су довела до
редукције растења и повећања садржаја суве материје, али су линије
реаговале различито на појединачне стресоре и њихове
концентрације. Линија Кф-Л1 је боље реаговала на ниже
концентрације NaCl, на подлози са 300 мМ NaCl обе линије су
показивале сличан пораст, али на вишој концентрацији од 400 мМ
линија Кф-Л1 је показала значајно мању толеранцију на повишени
салинитет у односу на линију Кф-Л2. Добијени резултати указују на
разлике међу тестираним линијама у толеранцији према стресу соли
и суше у in vitro условима, што ову методу чини ефикасном у
тестирању и селекцији толерантнијих линија карфиола и других
купусњача на осмотски стрес., The aim of the study was to examine the effect of osmotic stress (salt
and drought stress) on growth and dry matter content in cauliflower lines
grown in vitro. Two selected cauliflower lines were exposed in vitro to
salt stress and drought by culturing on MS medium with 0, 100, 200, 300
and 400 mM NaCl and resp. by culturing on MS medium with 0, 5, 10,
15 and 20% PEG. The fresh weight and the dry matter content were
measured at the beginning and after 8 days of treatment. Both stresses led
to a reduction in the growth and increase in dry matter content, but the
lines reacted differently to individual stressors and their concentrations.
The Kf-L1 line responded better to lower NaCl concentrations, on a
medium with 300 mM NaCl both lines showed a similar increase, but at a
higher concentration of 400 mM the Kf-L1 line showed significantly
lower tolerance to increased salinity compared to the Kf-L2line. Obtained
resultsindicate differences between the tested lines in tolerance to salt and
drought stress, which makes this method effective in testing and selecting
more tolerant lines of cauliflower and Brassica vegetables.",
publisher = "Смедеревска Паланка : Институт за повртарство",
journal = "Биотехнологија и савремени приступ у гајењу и оплемењивању биља, научно-стручни скуп са међународним учешћем, Зборник радова, Смедеревска Паланка, 15. децембар",
title = "Испитивање ефекта осмотског стреса код карфиола in vitro, Examination of the effect of osmotic stress in caulifiols in vitro",
pages = "144-137",
url = "https://hdl.handle.net/21.15107/rcub_rivec_429"
}
Павловић, С., Дамњановић, Ј., Гирек, З., Угриновић, М., Аџић, С., Милојевић, Ј.,& Брдар-Јокановић, М.. (2021). Испитивање ефекта осмотског стреса код карфиола in vitro. in Биотехнологија и савремени приступ у гајењу и оплемењивању биља, научно-стручни скуп са међународним учешћем, Зборник радова, Смедеревска Паланка, 15. децембар
Смедеревска Паланка : Институт за повртарство., 137-144.
https://hdl.handle.net/21.15107/rcub_rivec_429
Павловић С, Дамњановић Ј, Гирек З, Угриновић М, Аџић С, Милојевић Ј, Брдар-Јокановић М. Испитивање ефекта осмотског стреса код карфиола in vitro. in Биотехнологија и савремени приступ у гајењу и оплемењивању биља, научно-стручни скуп са међународним учешћем, Зборник радова, Смедеревска Паланка, 15. децембар. 2021;:137-144.
https://hdl.handle.net/21.15107/rcub_rivec_429 .
Павловић, Сузана, Дамњановић, Јелена, Гирек, Зденка, Угриновић, Милан, Аџић, Слађан, Милојевић, Јелена, Брдар-Јокановић, Милка, "Испитивање ефекта осмотског стреса код карфиола in vitro" in Биотехнологија и савремени приступ у гајењу и оплемењивању биља, научно-стручни скуп са међународним учешћем, Зборник радова, Смедеревска Паланка, 15. децембар (2021):137-144,
https://hdl.handle.net/21.15107/rcub_rivec_429 .

Variability in somatic embryo-forming capacity of spinach

Belić, Maja; Zdravković-Korać, Snežana; Uzelac, Branka; Calić, Dušica; Pavlović, Suzana; Milojević, Jelena

(Nature Research, Berlin, 2020)

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Calić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/214
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80-100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20-60% of regenerating explants. The explants from the majority of seedlings (52-100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10-20 (43.5%) up to>20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1-10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research, Berlin
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
ER  - 
@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Calić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80-100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20-60% of regenerating explants. The explants from the majority of seedlings (52-100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10-20 (43.5%) up to>20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1-10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research, Berlin",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9"
}
Belić, M., Zdravković-Korać, S., Uzelac, B., Calić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research, Berlin., 10(1).
https://doi.org/10.1038/s41598-020-76279-9
Belić M, Zdravković-Korać S, Uzelac B, Calić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1).
doi:10.1038/s41598-020-76279-9 .
Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Calić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020),
https://doi.org/10.1038/s41598-020-76279-9 . .
5
4

Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower

Pavlović, Suzana; Savić, Jelena; Milojević, Jelena; Vinterhalter, Branka; Girek, Zdenka; Adžić, Slađan; Zečević, Bogoljub; Banjac, Nevena

(Springer, Dordrecht, 2020)

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Savić, Jelena
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Girek, Zdenka
AU  - Adžić, Slađan
AU  - Zečević, Bogoljub
AU  - Banjac, Nevena
PY  - 2020
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/215
AB  - Key message The results underlined that constitutively expressingNPK1can significantly contribute to enhanced salt stress tolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerance cruciferous vegetable lines. Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression ofNPK1transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. TheAgrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring theNPK1and phosphinothricinN-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of bothNPK1andbartransgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level ofNPK1gene expression estimated by RT-qPCR, and the L2 line with the highestNPK1expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of thebargene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
EP  - 651
IS  - 3
SP  - 635
VL  - 143
DO  - 10.1007/s11240-020-01948-6
ER  - 
@article{
author = "Pavlović, Suzana and Savić, Jelena and Milojević, Jelena and Vinterhalter, Branka and Girek, Zdenka and Adžić, Slađan and Zečević, Bogoljub and Banjac, Nevena",
year = "2020",
abstract = "Key message The results underlined that constitutively expressingNPK1can significantly contribute to enhanced salt stress tolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerance cruciferous vegetable lines. Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression ofNPK1transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. TheAgrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring theNPK1and phosphinothricinN-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of bothNPK1andbartransgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level ofNPK1gene expression estimated by RT-qPCR, and the L2 line with the highestNPK1expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of thebargene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower",
pages = "651-635",
number = "3",
volume = "143",
doi = "10.1007/s11240-020-01948-6"
}
Pavlović, S., Savić, J., Milojević, J., Vinterhalter, B., Girek, Z., Adžić, S., Zečević, B.,& Banjac, N.. (2020). Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 143(3), 635-651.
https://doi.org/10.1007/s11240-020-01948-6
Pavlović S, Savić J, Milojević J, Vinterhalter B, Girek Z, Adžić S, Zečević B, Banjac N. Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell Tissue and Organ Culture. 2020;143(3):635-651.
doi:10.1007/s11240-020-01948-6 .
Pavlović, Suzana, Savić, Jelena, Milojević, Jelena, Vinterhalter, Branka, Girek, Zdenka, Adžić, Slađan, Zečević, Bogoljub, Banjac, Nevena, "Introduction of the Nicotiana protein kinase (NPK1) gene by combiningAgrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower" in Plant Cell Tissue and Organ Culture, 143, no. 3 (2020):635-651,
https://doi.org/10.1007/s11240-020-01948-6 . .
6
1
5

Yield components and yield of winter wheat in different years of research

Terzić, Dragan; Đekić, Vera; Milojević, Jelena; Branković, Snežana; Perišić, Vesna; Perišić, Vladimir; Đokić, Dragoslav

(Niš : Univerzitet u NIšu, Prirodno-matematički fakultet, Departman za biologiju, 2018)

TY  - JOUR
AU  - Terzić, Dragan
AU  - Đekić, Vera
AU  - Milojević, Jelena
AU  - Branković, Snežana
AU  - Perišić, Vesna
AU  - Perišić, Vladimir
AU  - Đokić, Dragoslav
PY  - 2018
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/796
AB  - Field trial with wheat varieties Perfekta, KG 56S, Aleksandra and Vizija was set on vertisol-type soil during the vegetation season 2010/11 and 2011/12. The aim of the research was to analyse the yield and grain yield components in four varieties of wheat cultivated on acid soil. The highest values of yield components and grain quality were established in the year with moderate temperatures and high precipitation in the vegetation year 2010/11. The KG 56S and Vizija varieties had the highest yield of grain, the highest number of plants and spikes per m2. The Perfekta variety showed the highest 1000 grain weight and grain weight per spike. The highest number of grains in the spike and the lowest average yield of grain during the research were recorded for the variety Aleksandra. A significant positive correlation between grain yield and grain weight per spike, the number of grains in the spike and the number of plants per m2 were established, as well as between the 1000 grain weight and the number of grains in the spike and the grain weight per spike.
AB  - Poljski ogled sa sortama pšenice Perfekta, Kg 56S, Aleksandra i Vizija postavljen je na zemljištu tipa vertisol tokom vegetacionih sezona 2010/11 i 2011/12 godine. Cilj istraživanja je bio da se kod četiri sorte pšenice gajene na kiselom zemljištu analizira prinos i komponente prinosa zrna. Najveće vrednosti komponenti prinosa i kvaliteta zrna ustanovljene su u godini sa umerenim temperaturama i velikom količinom padavina u vegetacionoj 2010/11 godini. Sorte KG 56S i Vizija imale su najveći prinos zrna, najveći broj biljaka i klasova po m2. Sorta Perfekta odlikovala se najvećom masom 1000 zrna i masom zrna po klasu. Najveći broj zrna u klasu i najmanji prosečan prinos zrna tokom istraživanja imala je sorta Aleksandra. Ustanovljena je značajno pozitivna korelacija između prinosa zrna i mase zrna po klasu, broja zrna po klasu i broja biljaka po m2, kao i između mase 1000 zrna i broja zrna po klasu i mase zrna po klasu.
PB  - Niš : Univerzitet u NIšu, Prirodno-matematički fakultet, Departman za biologiju
T2  - Biologica Nyssana
T1  - Yield components and yield of winter wheat in different years of research
EP  - 131
IS  - 2
SP  - 119
VL  - 9
DO  - 10.5281/zenodo.2538604
ER  - 
@article{
author = "Terzić, Dragan and Đekić, Vera and Milojević, Jelena and Branković, Snežana and Perišić, Vesna and Perišić, Vladimir and Đokić, Dragoslav",
year = "2018",
abstract = "Field trial with wheat varieties Perfekta, KG 56S, Aleksandra and Vizija was set on vertisol-type soil during the vegetation season 2010/11 and 2011/12. The aim of the research was to analyse the yield and grain yield components in four varieties of wheat cultivated on acid soil. The highest values of yield components and grain quality were established in the year with moderate temperatures and high precipitation in the vegetation year 2010/11. The KG 56S and Vizija varieties had the highest yield of grain, the highest number of plants and spikes per m2. The Perfekta variety showed the highest 1000 grain weight and grain weight per spike. The highest number of grains in the spike and the lowest average yield of grain during the research were recorded for the variety Aleksandra. A significant positive correlation between grain yield and grain weight per spike, the number of grains in the spike and the number of plants per m2 were established, as well as between the 1000 grain weight and the number of grains in the spike and the grain weight per spike., Poljski ogled sa sortama pšenice Perfekta, Kg 56S, Aleksandra i Vizija postavljen je na zemljištu tipa vertisol tokom vegetacionih sezona 2010/11 i 2011/12 godine. Cilj istraživanja je bio da se kod četiri sorte pšenice gajene na kiselom zemljištu analizira prinos i komponente prinosa zrna. Najveće vrednosti komponenti prinosa i kvaliteta zrna ustanovljene su u godini sa umerenim temperaturama i velikom količinom padavina u vegetacionoj 2010/11 godini. Sorte KG 56S i Vizija imale su najveći prinos zrna, najveći broj biljaka i klasova po m2. Sorta Perfekta odlikovala se najvećom masom 1000 zrna i masom zrna po klasu. Najveći broj zrna u klasu i najmanji prosečan prinos zrna tokom istraživanja imala je sorta Aleksandra. Ustanovljena je značajno pozitivna korelacija između prinosa zrna i mase zrna po klasu, broja zrna po klasu i broja biljaka po m2, kao i između mase 1000 zrna i broja zrna po klasu i mase zrna po klasu.",
publisher = "Niš : Univerzitet u NIšu, Prirodno-matematički fakultet, Departman za biologiju",
journal = "Biologica Nyssana",
title = "Yield components and yield of winter wheat in different years of research",
pages = "131-119",
number = "2",
volume = "9",
doi = "10.5281/zenodo.2538604"
}
Terzić, D., Đekić, V., Milojević, J., Branković, S., Perišić, V., Perišić, V.,& Đokić, D.. (2018). Yield components and yield of winter wheat in different years of research. in Biologica Nyssana
Niš : Univerzitet u NIšu, Prirodno-matematički fakultet, Departman za biologiju., 9(2), 119-131.
https://doi.org/10.5281/zenodo.2538604
Terzić D, Đekić V, Milojević J, Branković S, Perišić V, Perišić V, Đokić D. Yield components and yield of winter wheat in different years of research. in Biologica Nyssana. 2018;9(2):119-131.
doi:10.5281/zenodo.2538604 .
Terzić, Dragan, Đekić, Vera, Milojević, Jelena, Branković, Snežana, Perišić, Vesna, Perišić, Vladimir, Đokić, Dragoslav, "Yield components and yield of winter wheat in different years of research" in Biologica Nyssana, 9, no. 2 (2018):119-131,
https://doi.org/10.5281/zenodo.2538604 . .
1

Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.

Pavlović, Suzana; Adžić, Slađan; Zdravković, Jasmina; Cvikić, Dejan; Milojević, Jelena; Vinterhalter, Branka; Mitić, Nevena

(International Society for Horticultural Science, 2013)

TY  - CONF
AU  - Pavlović, Suzana
AU  - Adžić, Slađan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/139
AB  - An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.
EP  - 246
SP  - 239
VL  - 1005
DO  - 10.17660/ActaHortic.2013.1005.26
ER  - 
@conference{
author = "Pavlović, Suzana and Adžić, Slađan and Zdravković, Jasmina and Cvikić, Dejan and Milojević, Jelena and Vinterhalter, Branka and Mitić, Nevena",
year = "2013",
abstract = "An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.",
pages = "246-239",
volume = "1005",
doi = "10.17660/ActaHortic.2013.1005.26"
}
Pavlović, S., Adžić, S., Zdravković, J., Cvikić, D., Milojević, J., Vinterhalter, B.,& Mitić, N.. (2013). Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae
International Society for Horticultural Science., 1005, 239-246.
https://doi.org/10.17660/ActaHortic.2013.1005.26
Pavlović S, Adžić S, Zdravković J, Cvikić D, Milojević J, Vinterhalter B, Mitić N. Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae. 2013;1005:239-246.
doi:10.17660/ActaHortic.2013.1005.26 .
Pavlović, Suzana, Adžić, Slađan, Zdravković, Jasmina, Cvikić, Dejan, Milojević, Jelena, Vinterhalter, Branka, Mitić, Nevena, "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L." in Acta Horticulturae, 1005 (2013):239-246,
https://doi.org/10.17660/ActaHortic.2013.1005.26 . .
1

Long days promote somatic embryogenesis in spinach

Milojević, Jelena; Tubić, Ljiljana; Pavlović, Suzana; Mitić, Nevena; Calić, Dušica; Vinterhalter, Branka; Zdravković-Korać, Snežana

(Elsevier Science Bv, Amsterdam, 2012)

TY  - JOUR
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Pavlović, Suzana
AU  - Mitić, Nevena
AU  - Calić, Dušica
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/123
AB  - As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Long days promote somatic embryogenesis in spinach
EP  - 37
SP  - 32
VL  - 142
DO  - 10.1016/j.scienta.2012.04.020
ER  - 
@article{
author = "Milojević, Jelena and Tubić, Ljiljana and Pavlović, Suzana and Mitić, Nevena and Calić, Dušica and Vinterhalter, Branka and Zdravković-Korać, Snežana",
year = "2012",
abstract = "As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD. while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100 mu mol m(-2) s(-1) in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Long days promote somatic embryogenesis in spinach",
pages = "37-32",
volume = "142",
doi = "10.1016/j.scienta.2012.04.020"
}
Milojević, J., Tubić, L., Pavlović, S., Mitić, N., Calić, D., Vinterhalter, B.,& Zdravković-Korać, S.. (2012). Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 142, 32-37.
https://doi.org/10.1016/j.scienta.2012.04.020
Milojević J, Tubić L, Pavlović S, Mitić N, Calić D, Vinterhalter B, Zdravković-Korać S. Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae. 2012;142:32-37.
doi:10.1016/j.scienta.2012.04.020 .
Milojević, Jelena, Tubić, Ljiljana, Pavlović, Suzana, Mitić, Nevena, Calić, Dušica, Vinterhalter, Branka, Zdravković-Korać, Snežana, "Long days promote somatic embryogenesis in spinach" in Scientia Horticulturae, 142 (2012):32-37,
https://doi.org/10.1016/j.scienta.2012.04.020 . .
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