TY  - JOUR
AU  - Živković, Ivana
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Živković, Jelena
AU  - Bigović, Dubravka
AU  - Menković, Nebojša
AU  - Radin, Dragoslava
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4828
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/304
AB  - Medicinal plant extracts (MPE) have a broad antimicrobial activity, including antiviral effects. In our study, eleven dry extract of ten different medicinal plant prepared with two solvents (5%, dimethyl sulfoxide (DMSO) and 30% ethanol in 5% DMSO) in different concentrations have been examined for anti-noroviral activity. The reduction of norovirus > 1 log10 genome equivalents (GE) has been obtained with 1 mg/mL of Aronia melanocarpa leaf extract (AMLE), dried wine Prokupac (WPE) and Hypericum perforatum extract (HyPE). Some of MPE i.e. HyPE, Aronia melanocarpa fruit extract (AMFE) and Punica granatum (PPE) showed better activity when dissolved in 30% ethanol with 5% DMSO. All results of anti-noroviral activity of tested MPE which achieve  lt  0.5 log10 GE are considered as not effective.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Antiviral activity of medicinal plants extracts against foodborne norovirus
EP  - 34
IS  - 38
SP  - 31
DO  - 10.5937/leksir1838031Z
ER  - 

@article{
author = "Živković, Ivana and Šavikin, Katarina and Zdunić, Gordana and Živković, Jelena and Bigović, Dubravka and Menković, Nebojša and Radin, Dragoslava",
year = "2018",
abstract = "Medicinal plant extracts (MPE) have a broad antimicrobial activity, including antiviral effects. In our study, eleven dry extract of ten different medicinal plant prepared with two solvents (5%, dimethyl sulfoxide (DMSO) and 30% ethanol in 5% DMSO) in different concentrations have been examined for anti-noroviral activity. The reduction of norovirus > 1 log10 genome equivalents (GE) has been obtained with 1 mg/mL of Aronia melanocarpa leaf extract (AMLE), dried wine Prokupac (WPE) and Hypericum perforatum extract (HyPE). Some of MPE i.e. HyPE, Aronia melanocarpa fruit extract (AMFE) and Punica granatum (PPE) showed better activity when dissolved in 30% ethanol with 5% DMSO. All results of anti-noroviral activity of tested MPE which achieve  lt  0.5 log10 GE are considered as not effective.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Antiviral activity of medicinal plants extracts against foodborne norovirus",
pages = "34-31",
number = "38",
doi = "10.5937/leksir1838031Z"
}

Živković, I., Šavikin, K., Zdunić, G., Živković, J., Bigović, D., Menković, N.,& Radin, D.. (2018). Antiviral activity of medicinal plants extracts against foodborne norovirus. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(38), 31-34.
https://doi.org/10.5937/leksir1838031Z

Živković I, Šavikin K, Zdunić G, Živković J, Bigović D, Menković N, Radin D. Antiviral activity of medicinal plants extracts against foodborne norovirus. in Lekovite sirovine. 2018;(38):31-34.
doi:10.5937/leksir1838031Z .

Živković, Ivana, Šavikin, Katarina, Zdunić, Gordana, Živković, Jelena, Bigović, Dubravka, Menković, Nebojša, Radin, Dragoslava, "Antiviral activity of medicinal plants extracts against foodborne norovirus" in Lekovite sirovine, no. 38 (2018):31-34,
https://doi.org/10.5937/leksir1838031Z . .

TY  - CONF
AU  - Radin, Dragoslava
AU  - Živković, Ivana
PY  - 2016
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/305
AB  - Human  noroviruses  are  predominant  cause  of  epidemic  and  sporadic  food-borne  gastroenteritis  in industrialized  countries.  Priority  food  categories,  vehicles  of  viruses  in  many  reported  outbreaks, beside  shellfish  are  food  of  plant  origin  including  berries,  lettuce,  tomatoes  and  green  onions.  These items, as  well  as  deli  ham  are often  components  of  many  ready-to-eat foods.   The  aim  of  this  study  was  to  assess  the  effect  of  different  food  matrices  on  norovirus  detection.  Stool sample  with  previously  determined  concentration  of  norovirus  GII  particles,  was  used  to  inoculate surface  of  lettuce,  tomatoes,  green  onions  and  deli  ham  slices.  For  virus  RNA  extraction  the  direct method  was  used  followed  by  RT-qPCR  assay  with  SYBR  green  one-step  kit  and  degenerate  MON primers  for  the  detection  of  GII  norovirus.  Obtained  mean  Ct  values  and  Ct  alterations  calculated  for each  level  of  inoculation  revealed  that  tomato  had  no  inhibitory  effect  on  NoV  GII  detection,  followed by  one  log  decrease  of  RT-PCRU  due  to  properties  of  green  onions,  and  two  log  decrease  for  lettuce and  deli  ham  slices. The  adsorption  of  viruses  to  different  solid  surfaces  depends  on  their  complexity  and  food  surface factors  such  as  presence  of  hydrophobic  domains  and  acid/base  groups,  virus  specific  ligands,  etc. This  is  important  since  many  fruits  and  vegetables  have  complex  surfaces  that  prevent  the  removal  of viruses  by  simple  washing  and may  decrease desiccation  effects  that  could  lead  to virus  inactivation.
PB  - Novi Sad . University  of Novi Sad Institute  of  Food  Technology
C3  - Proceedings III  International Congress Food  Technology,  Quality  and Safety,  Novi Sad, 25-27th October  2016
T1  - The influence of the food surface  and  matrix  on norovirus detection by  RT-qPCR
EP  - 305
SP  - 300
UR  - https://hdl.handle.net/21.15107/rcub_rivec_305
ER  - 

@conference{
author = "Radin, Dragoslava and Živković, Ivana",
year = "2016",
abstract = "Human  noroviruses  are  predominant  cause  of  epidemic  and  sporadic  food-borne  gastroenteritis  in industrialized  countries.  Priority  food  categories,  vehicles  of  viruses  in  many  reported  outbreaks, beside  shellfish  are  food  of  plant  origin  including  berries,  lettuce,  tomatoes  and  green  onions.  These items, as  well  as  deli  ham  are often  components  of  many  ready-to-eat foods.   The  aim  of  this  study  was  to  assess  the  effect  of  different  food  matrices  on  norovirus  detection.  Stool sample  with  previously  determined  concentration  of  norovirus  GII  particles,  was  used  to  inoculate surface  of  lettuce,  tomatoes,  green  onions  and  deli  ham  slices.  For  virus  RNA  extraction  the  direct method  was  used  followed  by  RT-qPCR  assay  with  SYBR  green  one-step  kit  and  degenerate  MON primers  for  the  detection  of  GII  norovirus.  Obtained  mean  Ct  values  and  Ct  alterations  calculated  for each  level  of  inoculation  revealed  that  tomato  had  no  inhibitory  effect  on  NoV  GII  detection,  followed by  one  log  decrease  of  RT-PCRU  due  to  properties  of  green  onions,  and  two  log  decrease  for  lettuce and  deli  ham  slices. The  adsorption  of  viruses  to  different  solid  surfaces  depends  on  their  complexity  and  food  surface factors  such  as  presence  of  hydrophobic  domains  and  acid/base  groups,  virus  specific  ligands,  etc. This  is  important  since  many  fruits  and  vegetables  have  complex  surfaces  that  prevent  the  removal  of viruses  by  simple  washing  and may  decrease desiccation  effects  that  could  lead  to virus  inactivation.",
publisher = "Novi Sad . University  of Novi Sad Institute  of  Food  Technology",
journal = "Proceedings III  International Congress Food  Technology,  Quality  and Safety,  Novi Sad, 25-27th October  2016",
title = "The influence of the food surface  and  matrix  on norovirus detection by  RT-qPCR",
pages = "305-300",
url = "https://hdl.handle.net/21.15107/rcub_rivec_305"
}

Radin, D.,& Živković, I.. (2016). The influence of the food surface  and  matrix  on norovirus detection by  RT-qPCR. in Proceedings III  International Congress Food  Technology,  Quality  and Safety,  Novi Sad, 25-27th October  2016
Novi Sad . University  of Novi Sad Institute  of  Food  Technology., 300-305.
https://hdl.handle.net/21.15107/rcub_rivec_305

Radin D, Živković I. The influence of the food surface  and  matrix  on norovirus detection by  RT-qPCR. in Proceedings III  International Congress Food  Technology,  Quality  and Safety,  Novi Sad, 25-27th October  2016. 2016;:300-305.
https://hdl.handle.net/21.15107/rcub_rivec_305 .

Radin, Dragoslava, Živković, Ivana, "The influence of the food surface  and  matrix  on norovirus detection by  RT-qPCR" in Proceedings III  International Congress Food  Technology,  Quality  and Safety,  Novi Sad, 25-27th October  2016 (2016):300-305,
https://hdl.handle.net/21.15107/rcub_rivec_305 .