TY  - JOUR
AU  - Jošić, Dragana
AU  - Cvikić, Dejan
AU  - Zdravković, Jasmina
AU  - Kuzmanović, Đorđe
AU  - Zdravković, Milan
AU  - Knežević, Magdalena
AU  - Pavlović, Snežana
PY  - 2017
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/194
AB  - The inhibitory effect of indigenous Pseudomonas strains on Fusarium spp. isolated from seeds of a new variety of carrot - 'Vizija' and their effects on seed germination were observed. Pseudomonas spp. strains were applied as bacterial suspensions (culture, 106 CFU mL-1) and cell-free supernatant (CFSa -106 and CFSb -108 CFU mL-1) fractions by sowing seeds during 7 (I) and 14 (II) days of incubation. The germination of control 'Vizija' seeds was 19% after the first and 40% after the second incubation period. Pseudomonas chlororaphis Q16 strain exhibited statistically significant increases in seed germination with all applied fractions and incubation periods, showing values of 43-62% and 55.5-91%, i.e. increments of 24-43% and 15.5-51% compared to the control, after 7 and 14 days of incubation, respectively. P. chlororaphis K35 showed lower but significantly different seed germination values (38-67%) for all variants, except for 7 days old culture. Pseudomonas sp. Ek1 had weaker seed germination potential, showing statistically significant increment only for CFSa,b (I) and CFSb (II) fractions. All tested Pseudomonas strains inhibited the growth of three Fusarium species isolated from 'Vizija' seeds: F. solani, F. oxysporum and F. subglutinans. Natural infection was observed in 20% (I) and 54% (II) of 'Vizija' seeds. P. chlororaphis K35 was the most efficient antifungal strain, reducing seed infection 97.5-100%, followed by Q16 with 95-100%, showing no statistically significant mutual difference. Pseudomonas sp. Ek1 showed a weaker antifungal activity and reduced seed infection by 85-96.75%. The application of P. chlororaphis Q16 and K35 as strains effective in improvement of carrot seed germination and growth inhibition of the seed pathogens F. solani, F. oxysporum and F. subglutinans, can be further tested in carrot production for more beneficial effects.
AB  - Ispitivan je inhibitorni efekat autohtonih Pseudomonas sojeva na gljive Fusarium spp. izolovane sa semena šargarepe sorte 'Vizija' i njihov uticaj na klijavost semena. Pseudomonas spp. sojevi su primenjeni u vidu bakterijske suspenzije (kulture, 106 CFU mL-1) i supernatanta (CFSa -106 i CFSb -108 CFU mL-1) potapanjem semena, a potom inkubirani tokom 7 (I) i 14 (II) dana. Klijavost semena 'Vizije' u kontroli iznosila je 19% posle prvog i 40% posle drugog perioda inkubacije. P. chlororaphis Q16 soj je uzrokovao statistički značajno povećanje klijavosti semena pri primeni svih frakcija i tokom oba inkubaciona perioda, dostižući 43-62% i 55.5-91% klijavosti, što je povećanje od 24-43% i 15.5-51% u poređenju sa kontrolom, posle 7 i 14 dana inkubacije. P. chlororaphis K35 je uslovio manje, ali statistički značajne vrednosti klijavosti semena (38-67%) za sve varijante, osim za kulturu posle 7 dana. Pseudomonas sp. Ek1 je pokazao slabiji potencijal klijavosti, sa statistički značajnim povećanjem samo za CFSa,b (I) i CFSb (II) frakcije. Svi ispitani Pseudomonas sojevi su inhibirali tri vrste gljiva iz roda Fusarium koje su izolovane sa semena 'Vizije': F. solani, F. oxysporum i F. subglutinans. Prirodna infekcija semena je uočena kod 20% (I) i 54% (II) semena 'Vizije' u kontrolnoj varijanti. P. chlororaphis K35 je ispoljio najače antifungalno dejstvo, sa smanjenjem infekcije semena 97.5-100%, a zatim soj Q16 sa 95-100%, bez statističke značajnosti među njima. Pseudomonas sp. Ek1 je ispoljio slabiju antifungalnu aktivnost i redukovao infekciju semena 85-96.75%. Primena P. chlororaphis Q16 i K35, kao sojeva koji efikasno povećavaju klijavost semena šargarepe i inhibiraju rast patogena semena F. solani, F. oxysporum i F. subglutinans, biće dalje testirana u proizvodnji šargarepe kako bi se procenili ostali pozitivni efekti ovih sojeva.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.
T1  - Daucus carrota L. - klijavost semena i prirodna infekcija semena Fusarium spp. pod uticajem Pseudomonas spp.
EP  - 195
IS  - 3-4
SP  - 189
VL  - 32
DO  - 10.2298/PIF1704189J
ER  - 

@article{
author = "Jošić, Dragana and Cvikić, Dejan and Zdravković, Jasmina and Kuzmanović, Đorđe and Zdravković, Milan and Knežević, Magdalena and Pavlović, Snežana",
year = "2017",
abstract = "The inhibitory effect of indigenous Pseudomonas strains on Fusarium spp. isolated from seeds of a new variety of carrot - 'Vizija' and their effects on seed germination were observed. Pseudomonas spp. strains were applied as bacterial suspensions (culture, 106 CFU mL-1) and cell-free supernatant (CFSa -106 and CFSb -108 CFU mL-1) fractions by sowing seeds during 7 (I) and 14 (II) days of incubation. The germination of control 'Vizija' seeds was 19% after the first and 40% after the second incubation period. Pseudomonas chlororaphis Q16 strain exhibited statistically significant increases in seed germination with all applied fractions and incubation periods, showing values of 43-62% and 55.5-91%, i.e. increments of 24-43% and 15.5-51% compared to the control, after 7 and 14 days of incubation, respectively. P. chlororaphis K35 showed lower but significantly different seed germination values (38-67%) for all variants, except for 7 days old culture. Pseudomonas sp. Ek1 had weaker seed germination potential, showing statistically significant increment only for CFSa,b (I) and CFSb (II) fractions. All tested Pseudomonas strains inhibited the growth of three Fusarium species isolated from 'Vizija' seeds: F. solani, F. oxysporum and F. subglutinans. Natural infection was observed in 20% (I) and 54% (II) of 'Vizija' seeds. P. chlororaphis K35 was the most efficient antifungal strain, reducing seed infection 97.5-100%, followed by Q16 with 95-100%, showing no statistically significant mutual difference. Pseudomonas sp. Ek1 showed a weaker antifungal activity and reduced seed infection by 85-96.75%. The application of P. chlororaphis Q16 and K35 as strains effective in improvement of carrot seed germination and growth inhibition of the seed pathogens F. solani, F. oxysporum and F. subglutinans, can be further tested in carrot production for more beneficial effects., Ispitivan je inhibitorni efekat autohtonih Pseudomonas sojeva na gljive Fusarium spp. izolovane sa semena šargarepe sorte 'Vizija' i njihov uticaj na klijavost semena. Pseudomonas spp. sojevi su primenjeni u vidu bakterijske suspenzije (kulture, 106 CFU mL-1) i supernatanta (CFSa -106 i CFSb -108 CFU mL-1) potapanjem semena, a potom inkubirani tokom 7 (I) i 14 (II) dana. Klijavost semena 'Vizije' u kontroli iznosila je 19% posle prvog i 40% posle drugog perioda inkubacije. P. chlororaphis Q16 soj je uzrokovao statistički značajno povećanje klijavosti semena pri primeni svih frakcija i tokom oba inkubaciona perioda, dostižući 43-62% i 55.5-91% klijavosti, što je povećanje od 24-43% i 15.5-51% u poređenju sa kontrolom, posle 7 i 14 dana inkubacije. P. chlororaphis K35 je uslovio manje, ali statistički značajne vrednosti klijavosti semena (38-67%) za sve varijante, osim za kulturu posle 7 dana. Pseudomonas sp. Ek1 je pokazao slabiji potencijal klijavosti, sa statistički značajnim povećanjem samo za CFSa,b (I) i CFSb (II) frakcije. Svi ispitani Pseudomonas sojevi su inhibirali tri vrste gljiva iz roda Fusarium koje su izolovane sa semena 'Vizije': F. solani, F. oxysporum i F. subglutinans. Prirodna infekcija semena je uočena kod 20% (I) i 54% (II) semena 'Vizije' u kontrolnoj varijanti. P. chlororaphis K35 je ispoljio najače antifungalno dejstvo, sa smanjenjem infekcije semena 97.5-100%, a zatim soj Q16 sa 95-100%, bez statističke značajnosti među njima. Pseudomonas sp. Ek1 je ispoljio slabiju antifungalnu aktivnost i redukovao infekciju semena 85-96.75%. Primena P. chlororaphis Q16 i K35, kao sojeva koji efikasno povećavaju klijavost semena šargarepe i inhibiraju rast patogena semena F. solani, F. oxysporum i F. subglutinans, biće dalje testirana u proizvodnji šargarepe kako bi se procenili ostali pozitivni efekti ovih sojeva.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp., Daucus carrota L. - klijavost semena i prirodna infekcija semena Fusarium spp. pod uticajem Pseudomonas spp.",
pages = "195-189",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704189J"
}

Jošić, D., Cvikić, D., Zdravković, J., Kuzmanović, Đ., Zdravković, M., Knežević, M.,& Pavlović, S.. (2017). Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 32(3-4), 189-195.
https://doi.org/10.2298/PIF1704189J

Jošić D, Cvikić D, Zdravković J, Kuzmanović Đ, Zdravković M, Knežević M, Pavlović S. Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.. in Pesticidi i fitomedicina. 2017;32(3-4):189-195.
doi:10.2298/PIF1704189J .

Jošić, Dragana, Cvikić, Dejan, Zdravković, Jasmina, Kuzmanović, Đorđe, Zdravković, Milan, Knežević, Magdalena, Pavlović, Snežana, "Daucus carrota L.: Seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp." in Pesticidi i fitomedicina, 32, no. 3-4 (2017):189-195,
https://doi.org/10.2298/PIF1704189J . .

TY  - JOUR
AU  - Pavlović, Radoš
AU  - Mladenović, Jelena
AU  - Pavlović, Nenad
AU  - Zdravković, Milan
AU  - Jošić, Dragana
AU  - Zdravković, Jasmina
PY  - 2017
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/187
AB  - The aim of this study was to choose the genotypes of industrial tomato for the content of bioactive components (ascorbic acid, beta-carotene, lycopene, total phenols and flavonoids) in fruits and its preservation during thermal treatment (by drying with parallel warm air at 60 degrees C) and making of tomato juice (by pasteurization - cooking at 100 degrees C for 7 minutes). For this research, a comparative trial has been set up with 7 genotypes, 1 commercial variety (SP-109) and 6 selected lines (SPP, SPSM, SPRZ, SPRM-20, S-60 and SPO) of high inbreeding generations. Experimental design has been done according to standard method of growing industrial tomato in random block system with three replications. By analysing the cumulative results of all researched genotypes for processing industry, the best for drying and fresh consumption was SPRZ and for juice extraction, SPSM was the best line.
PB  - Wydawnictwo Akad Rolniczej W Lublinie, Lublin
T2  - Acta Scientiarum Polonorum-Hortorum Cultus
T1  - Antioxidant nutritional quality and the effect of thermal treatments on selected processing tomato lines
EP  - 128
IS  - 3
SP  - 119
VL  - 16
DO  - 10.24326/asphc.2017.3.12
ER  - 

@article{
author = "Pavlović, Radoš and Mladenović, Jelena and Pavlović, Nenad and Zdravković, Milan and Jošić, Dragana and Zdravković, Jasmina",
year = "2017",
abstract = "The aim of this study was to choose the genotypes of industrial tomato for the content of bioactive components (ascorbic acid, beta-carotene, lycopene, total phenols and flavonoids) in fruits and its preservation during thermal treatment (by drying with parallel warm air at 60 degrees C) and making of tomato juice (by pasteurization - cooking at 100 degrees C for 7 minutes). For this research, a comparative trial has been set up with 7 genotypes, 1 commercial variety (SP-109) and 6 selected lines (SPP, SPSM, SPRZ, SPRM-20, S-60 and SPO) of high inbreeding generations. Experimental design has been done according to standard method of growing industrial tomato in random block system with three replications. By analysing the cumulative results of all researched genotypes for processing industry, the best for drying and fresh consumption was SPRZ and for juice extraction, SPSM was the best line.",
publisher = "Wydawnictwo Akad Rolniczej W Lublinie, Lublin",
journal = "Acta Scientiarum Polonorum-Hortorum Cultus",
title = "Antioxidant nutritional quality and the effect of thermal treatments on selected processing tomato lines",
pages = "128-119",
number = "3",
volume = "16",
doi = "10.24326/asphc.2017.3.12"
}

Pavlović, R., Mladenović, J., Pavlović, N., Zdravković, M., Jošić, D.,& Zdravković, J.. (2017). Antioxidant nutritional quality and the effect of thermal treatments on selected processing tomato lines. in Acta Scientiarum Polonorum-Hortorum Cultus
Wydawnictwo Akad Rolniczej W Lublinie, Lublin., 16(3), 119-128.
https://doi.org/10.24326/asphc.2017.3.12

Pavlović R, Mladenović J, Pavlović N, Zdravković M, Jošić D, Zdravković J. Antioxidant nutritional quality and the effect of thermal treatments on selected processing tomato lines. in Acta Scientiarum Polonorum-Hortorum Cultus. 2017;16(3):119-128.
doi:10.24326/asphc.2017.3.12 .

Pavlović, Radoš, Mladenović, Jelena, Pavlović, Nenad, Zdravković, Milan, Jošić, Dragana, Zdravković, Jasmina, "Antioxidant nutritional quality and the effect of thermal treatments on selected processing tomato lines" in Acta Scientiarum Polonorum-Hortorum Cultus, 16, no. 3 (2017):119-128,
https://doi.org/10.24326/asphc.2017.3.12 . .

TY  - JOUR
AU  - Zdravković, Jasmina
AU  - Ugrinović, Milan
AU  - Zdravković, Milan
AU  - Đorđević, Slaviša
AU  - Pavlović, Snežana
AU  - Jošić, Dragana
PY  - 2015
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/172
AB  - Cucumber (Cucumis sativus L) is an important member of the Cucurbitaceae family. Production of healthy nursery is necessary for high-quality production of this crop in greenhouses and in fields. With the idea of minimizing the use of pesticides and mineral fertilizers to preserve soil quality, we investigated the effects of plant growth promoting bacteria (PGPB) on growth promotion and protection of cucumber plants from phytopathogenic fungi. The effects of Pseudomonas spp. strains with different antifungal activities and Bacillus sp. Q10 strain with PGP activity were tested on cucumber plants. Antagonistic activity of Pseudomonas spp. against the growth of several phytopathogenic fungi isolated from cucumber: F. acuminatum, B. cinerea and A. niger, was observed. The influences of overnight cultures, supernatants and heat-stable antifungal factors were tested on the phytopathogenic fungi in vitro. Pseudomonas sp. K35 and K24 strains were more effective than P. chlororaphis Q16 and Pseudomonas sp. K27, showing 70-80% of fungal growth inhibition regardless of culture or fraction applied. The good antagonists that belong to pseudomonads and Bacillus sp. Q10 strain were used as mixtures for estimation of plant growth and health promoting effects on cucumber plants. Growth dynamics differed depending on the applied strain of Pseudomonas sp. The M3 treatment (a mixture of Bacillus sp. Q10 and P. chlororaphis Q16) stimulated the initial phase of growth, while M4 (a mixture of Bacillus sp. Q10 and Pseudomonas sp. K24) resulted in the maximal height at the final measurement. Significant differences in leaf and plant weight (M4), and leaf weight (M5, containing K35 strain) were found after the treatments. No significant differences in chlorophyll and NBI level were observed in any of the tested combinations. The obtained results suggested that M3 was suitable for stimulation of the early phase of cucumber growth, while the mixtures M4 and M5 improved plant protection and stimulated the later phases of cucumber growth.
AB  - Krastavac (Cucumis sativus L) je važan predstavnik familije Cucurbitaceae, a proizvodnja zdravog rasada je nephodna za visoku produktivnost u plastenicima i na otvorenom polju. Da bi se umanjila primena pesticida i mineralnih đubriva i pri tome sačuvalo zemljište, ispitan je uticaj bakterija stimulatora biljnog rasta (PGPB) na rast biljaka i zaštitu od patogena. Primenjeni su sojevi Pseudomonas spp. sa različitim antimikoznim delovanjem i Bacillus sp. soj Q10 sa PGP aktivnošću. Praćena je antagonistička aktivnost Pseudomonas spp. na fitopatogene gljive izolovane sa biljaka krastavca: F. acuminatum, B. cinerea i A. niger. Testiran je uticaj prekonoćne kulture bakterija, supernatanta i termostabilnih antifungalnih faktora na rast ovih fitopatogena. Pseudomonas sp. K35 i K24 sojevi, koji pokazuju 70-80% inhibicije rasta gljiva bez obzira na primenjenu kulturu ili frakciju, efektivniji su od P. chlororaphis Q16 i Pseudomonas sp. K27. Sojevi Pseudomonas spp., koji su ispoljili visok stepen antagonizma, kombinovani su sa sojem Bacillus sp. Q10 i procenjen je uticaj na rast i zdravstveno stanje biljaka krastavca. U zavisnosti od primenjenog soja Pseudomonas sp., razlikovala se dinamika rasta krastavca. Tretman M3 - kombinacija Bacillus sp. Q10 i P. chlororaphis Q16 uticala je na početnu fazu porasta biljaka, dok je treatman M4 - kombinacija Bacillus sp. Q10 i Pseudomonas sp. K24, imala najviše uticaja na visinu biljke na kraju merenja. Uočene su značajne razlike za masu lista i masu biljke (M4) i masu lista (M5 sa sojem K35), dok sadržaj hlorofila i nivo NBI nisu imali značajne razlike kod svih ispitivanih kombinacija. Dobijeni rezultati ukazuju da je M3 pogodan za rane faze razvoja biljke, a kombinacije M4 i M5 pogodne su za zaštitu biljaka i u kasnijim fazama porasta biljaka. Potpuna ekspresija PGP svojstava za M4 i M5 može se utvrditi tek posle testiranja na siromašnim supstratima, koji će biti upoređeni sa rezultaima dobijenim na supstratima sa dovoljno hranljivih materija.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber
T1  - In vitro i in vivo efekti Pseudomonas spp. i Bacillus sp. na Fusarium acuminatum, Botrytis cinerea i Aspergillus niger na krastavcu
EP  - 178
IS  - 3
SP  - 169
VL  - 30
DO  - 10.2298/pif1503169z
ER  - 

@article{
author = "Zdravković, Jasmina and Ugrinović, Milan and Zdravković, Milan and Đorđević, Slaviša and Pavlović, Snežana and Jošić, Dragana",
year = "2015",
abstract = "Cucumber (Cucumis sativus L) is an important member of the Cucurbitaceae family. Production of healthy nursery is necessary for high-quality production of this crop in greenhouses and in fields. With the idea of minimizing the use of pesticides and mineral fertilizers to preserve soil quality, we investigated the effects of plant growth promoting bacteria (PGPB) on growth promotion and protection of cucumber plants from phytopathogenic fungi. The effects of Pseudomonas spp. strains with different antifungal activities and Bacillus sp. Q10 strain with PGP activity were tested on cucumber plants. Antagonistic activity of Pseudomonas spp. against the growth of several phytopathogenic fungi isolated from cucumber: F. acuminatum, B. cinerea and A. niger, was observed. The influences of overnight cultures, supernatants and heat-stable antifungal factors were tested on the phytopathogenic fungi in vitro. Pseudomonas sp. K35 and K24 strains were more effective than P. chlororaphis Q16 and Pseudomonas sp. K27, showing 70-80% of fungal growth inhibition regardless of culture or fraction applied. The good antagonists that belong to pseudomonads and Bacillus sp. Q10 strain were used as mixtures for estimation of plant growth and health promoting effects on cucumber plants. Growth dynamics differed depending on the applied strain of Pseudomonas sp. The M3 treatment (a mixture of Bacillus sp. Q10 and P. chlororaphis Q16) stimulated the initial phase of growth, while M4 (a mixture of Bacillus sp. Q10 and Pseudomonas sp. K24) resulted in the maximal height at the final measurement. Significant differences in leaf and plant weight (M4), and leaf weight (M5, containing K35 strain) were found after the treatments. No significant differences in chlorophyll and NBI level were observed in any of the tested combinations. The obtained results suggested that M3 was suitable for stimulation of the early phase of cucumber growth, while the mixtures M4 and M5 improved plant protection and stimulated the later phases of cucumber growth., Krastavac (Cucumis sativus L) je važan predstavnik familije Cucurbitaceae, a proizvodnja zdravog rasada je nephodna za visoku produktivnost u plastenicima i na otvorenom polju. Da bi se umanjila primena pesticida i mineralnih đubriva i pri tome sačuvalo zemljište, ispitan je uticaj bakterija stimulatora biljnog rasta (PGPB) na rast biljaka i zaštitu od patogena. Primenjeni su sojevi Pseudomonas spp. sa različitim antimikoznim delovanjem i Bacillus sp. soj Q10 sa PGP aktivnošću. Praćena je antagonistička aktivnost Pseudomonas spp. na fitopatogene gljive izolovane sa biljaka krastavca: F. acuminatum, B. cinerea i A. niger. Testiran je uticaj prekonoćne kulture bakterija, supernatanta i termostabilnih antifungalnih faktora na rast ovih fitopatogena. Pseudomonas sp. K35 i K24 sojevi, koji pokazuju 70-80% inhibicije rasta gljiva bez obzira na primenjenu kulturu ili frakciju, efektivniji su od P. chlororaphis Q16 i Pseudomonas sp. K27. Sojevi Pseudomonas spp., koji su ispoljili visok stepen antagonizma, kombinovani su sa sojem Bacillus sp. Q10 i procenjen je uticaj na rast i zdravstveno stanje biljaka krastavca. U zavisnosti od primenjenog soja Pseudomonas sp., razlikovala se dinamika rasta krastavca. Tretman M3 - kombinacija Bacillus sp. Q10 i P. chlororaphis Q16 uticala je na početnu fazu porasta biljaka, dok je treatman M4 - kombinacija Bacillus sp. Q10 i Pseudomonas sp. K24, imala najviše uticaja na visinu biljke na kraju merenja. Uočene su značajne razlike za masu lista i masu biljke (M4) i masu lista (M5 sa sojem K35), dok sadržaj hlorofila i nivo NBI nisu imali značajne razlike kod svih ispitivanih kombinacija. Dobijeni rezultati ukazuju da je M3 pogodan za rane faze razvoja biljke, a kombinacije M4 i M5 pogodne su za zaštitu biljaka i u kasnijim fazama porasta biljaka. Potpuna ekspresija PGP svojstava za M4 i M5 može se utvrditi tek posle testiranja na siromašnim supstratima, koji će biti upoređeni sa rezultaima dobijenim na supstratima sa dovoljno hranljivih materija.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber, In vitro i in vivo efekti Pseudomonas spp. i Bacillus sp. na Fusarium acuminatum, Botrytis cinerea i Aspergillus niger na krastavcu",
pages = "178-169",
number = "3",
volume = "30",
doi = "10.2298/pif1503169z"
}

Zdravković, J., Ugrinović, M., Zdravković, M., Đorđević, S., Pavlović, S.,& Jošić, D.. (2015). In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 30(3), 169-178.
https://doi.org/10.2298/pif1503169z

Zdravković J, Ugrinović M, Zdravković M, Đorđević S, Pavlović S, Jošić D. In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber. in Pesticidi i fitomedicina. 2015;30(3):169-178.
doi:10.2298/pif1503169z .

Zdravković, Jasmina, Ugrinović, Milan, Zdravković, Milan, Đorđević, Slaviša, Pavlović, Snežana, Jošić, Dragana, "In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber" in Pesticidi i fitomedicina, 30, no. 3 (2015):169-178,
https://doi.org/10.2298/pif1503169z . .

TY  - JOUR
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Kojić, S.
AU  - Pivić, R.
AU  - Stanojković-Sebić, A.
AU  - Zdravković, Milan
AU  - Pavlović, Snežana
PY  - 2015
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/173
AB  - Sweet William (Dianthus barbatus, Caryophyllaceae) is a biennial or short-lived perennial plant native to southern Europe, from the Pyrenees to the Carpathians and the Balkans. During the summers of 2012 and 2013, phytoplasma-like symptoms were observed on D. barbatus plants on a Serbian plantation (Pancevo, 44°51′49″ N, 20°39′33″ E, 80 m ASL). Only seven symptomatic plants were observed in the summer of 2012. Disease incidence in 2013 was estimated to be less than 1% but increased during 2014 to 4%. Affected plants, showing symptoms of leaf reddening, malformation, and proliferation; flower bud deficiency; and abnormal shoot production, were tested for phytoplasmas. Samples were collected from seven symptomatic and three symptomless plants each year (20 samples), and total nucleic acid was extracted from midrib tissue using a method that includes a phytoplasma enrichment step and DNA purification by chloroform/phenol (3). Oligonucleotide primers specific to the phytoplasma 16S to 23S rRNA intergenic spacer region were used in polymerase chain reaction (PCR) assays on DNA extracted from Sweet William plants (1,3). Using phytoplasma universal primer pairs P1/P7 and P1/16S-Sr, phytoplasma-specific 1.8- and 1.5-kb amplicons were obtained from four and six symptomatic plants collected in 2012 and 2013, respectively. Nested PCR with R16F2n/R2 primers yielded ~1.2-kb amplicons from DNAs of all symptomatic plants (1). No amplicon was generated in PCRs conducted with DNA templates from symptomless plants. Restriction fragment length polymorphism (RFLP) analysis of amplified 1.2-kb fragments was performed using four endonucleases (AluI, Tru1I, HhaI, and HpaII). Comparative analysis was done using RFLP patterns of Stolbur (Stol), Aster Yellows (AY), Flavescence Doree-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas. PCR-RFLP patterns from tested samples were identical to those of the Stol reference strain, indicating that diseased Sweet William was affected by phytoplasma belonging to the 16SrXII-A (Stolbur) group. The sequence of a 1.2-kb rDNA PCR product derived from sample Tk9 (deposited under accession number KM401436 in NCBI GenBank) showed the closest identity (100%) to those of Bulgarian corn (KF907506.1), Iranian ‘Bois Noir’ (KJ637208.1), and two Serbian phytoplasmas (KJ174507.1 from Calendula officinalis and KF614623.1 from Paeonia tenuifolia), all belonging to the ‘Candidatus Phytoplasma solani’ Stolbur subgroup. Previously, Aster Yellows Phytoplasma (16SrI) had been detected in two Dianthus species: D. barbatus (Sweet William) and D. caryophyllus (carnation) (2). This is the first record of the 16SrXII-A phytoplasma subgroup being associated with yellowing and reddening of D. barbatus in Serbia. The Stolbur phytoplasma occurrence on Sweet William is significant for the management of the disease in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.
EP  - 283
IS  - 2
SP  - 283
VL  - 99
DO  - 10.1094/PDIS-08-14-0875-PDN
ER  - 

@article{
author = "Jošić, Dragana and Starović, Mira and Kojić, S. and Pivić, R. and Stanojković-Sebić, A. and Zdravković, Milan and Pavlović, Snežana",
year = "2015",
abstract = "Sweet William (Dianthus barbatus, Caryophyllaceae) is a biennial or short-lived perennial plant native to southern Europe, from the Pyrenees to the Carpathians and the Balkans. During the summers of 2012 and 2013, phytoplasma-like symptoms were observed on D. barbatus plants on a Serbian plantation (Pancevo, 44°51′49″ N, 20°39′33″ E, 80 m ASL). Only seven symptomatic plants were observed in the summer of 2012. Disease incidence in 2013 was estimated to be less than 1% but increased during 2014 to 4%. Affected plants, showing symptoms of leaf reddening, malformation, and proliferation; flower bud deficiency; and abnormal shoot production, were tested for phytoplasmas. Samples were collected from seven symptomatic and three symptomless plants each year (20 samples), and total nucleic acid was extracted from midrib tissue using a method that includes a phytoplasma enrichment step and DNA purification by chloroform/phenol (3). Oligonucleotide primers specific to the phytoplasma 16S to 23S rRNA intergenic spacer region were used in polymerase chain reaction (PCR) assays on DNA extracted from Sweet William plants (1,3). Using phytoplasma universal primer pairs P1/P7 and P1/16S-Sr, phytoplasma-specific 1.8- and 1.5-kb amplicons were obtained from four and six symptomatic plants collected in 2012 and 2013, respectively. Nested PCR with R16F2n/R2 primers yielded ~1.2-kb amplicons from DNAs of all symptomatic plants (1). No amplicon was generated in PCRs conducted with DNA templates from symptomless plants. Restriction fragment length polymorphism (RFLP) analysis of amplified 1.2-kb fragments was performed using four endonucleases (AluI, Tru1I, HhaI, and HpaII). Comparative analysis was done using RFLP patterns of Stolbur (Stol), Aster Yellows (AY), Flavescence Doree-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas. PCR-RFLP patterns from tested samples were identical to those of the Stol reference strain, indicating that diseased Sweet William was affected by phytoplasma belonging to the 16SrXII-A (Stolbur) group. The sequence of a 1.2-kb rDNA PCR product derived from sample Tk9 (deposited under accession number KM401436 in NCBI GenBank) showed the closest identity (100%) to those of Bulgarian corn (KF907506.1), Iranian ‘Bois Noir’ (KJ637208.1), and two Serbian phytoplasmas (KJ174507.1 from Calendula officinalis and KF614623.1 from Paeonia tenuifolia), all belonging to the ‘Candidatus Phytoplasma solani’ Stolbur subgroup. Previously, Aster Yellows Phytoplasma (16SrI) had been detected in two Dianthus species: D. barbatus (Sweet William) and D. caryophyllus (carnation) (2). This is the first record of the 16SrXII-A phytoplasma subgroup being associated with yellowing and reddening of D. barbatus in Serbia. The Stolbur phytoplasma occurrence on Sweet William is significant for the management of the disease in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.",
pages = "283-283",
number = "2",
volume = "99",
doi = "10.1094/PDIS-08-14-0875-PDN"
}

Jošić, D., Starović, M., Kojić, S., Pivić, R., Stanojković-Sebić, A., Zdravković, M.,& Pavlović, S.. (2015). Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(2), 283-283.
https://doi.org/10.1094/PDIS-08-14-0875-PDN

Jošić D, Starović M, Kojić S, Pivić R, Stanojković-Sebić A, Zdravković M, Pavlović S. Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia.. in Plant Disease. 2015;99(2):283-283.
doi:10.1094/PDIS-08-14-0875-PDN .

Jošić, Dragana, Starović, Mira, Kojić, S., Pivić, R., Stanojković-Sebić, A., Zdravković, Milan, Pavlović, Snežana, "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia." in Plant Disease, 99, no. 2 (2015):283-283,
https://doi.org/10.1094/PDIS-08-14-0875-PDN . .

TY  - JOUR
AU  - Ugrinović, Milan
AU  - Mijatović, Mirjana
AU  - Zdravković, Jasmina
AU  - Girek, Zdenka
AU  - Kuzmanović, Đorđe
AU  - Rasulić, Nataša
AU  - Jošić, Dragana
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/160
AB  - The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants' yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)(5)-PCR fingerprinting showed presence of six different patterns on the 92% similarity level.
PB  - Scientific Agricultural Soc Finland, Univ Helsinski
T2  - Agricultural and Food Science
T1  - Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia
EP  - 185
IS  - 3
SP  - 173
VL  - 23
DO  - 10.23986/afsci.41385
ER  - 

@article{
author = "Ugrinović, Milan and Mijatović, Mirjana and Zdravković, Jasmina and Girek, Zdenka and Kuzmanović, Đorđe and Rasulić, Nataša and Jošić, Dragana",
year = "2014",
abstract = "The impact of intercropping green bean (Phaseolus vulgaris L.) with red beet (Beta vulgaris L. var. rubra) and radish (Raphanus sativus L.), two non-legume plants, on the plants' yields, as well as the effect on occurrence and enumeration of microorganisms in the rhizosphere was studied. The intercrop efficacy evaluation, using Land equivalent ratio, revealed values above 1.0 for all intercropped treatments. Diversity of rhizobia from green bean nodules under different intercropping and fertilizing conditions was observed. On the basis of morphological and biochemical characteristics, 67 out of 158 isolates from green bean roots were selected as rhizobia (42.4%), confirmed by detection of 780 bp nifH gene fragments in nifH-PCR, and then clustered in 27 phenotype patterns. Production of exopolysaccharide succinoglycan was observed in 23 rhizobial isolates, while 6 were detected to solubilize tricalcium phosphate. Screening of genetic diversity using (GTG)(5)-PCR fingerprinting showed presence of six different patterns on the 92% similarity level.",
publisher = "Scientific Agricultural Soc Finland, Univ Helsinski",
journal = "Agricultural and Food Science",
title = "Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia",
pages = "185-173",
number = "3",
volume = "23",
doi = "10.23986/afsci.41385"
}

Ugrinović, M., Mijatović, M., Zdravković, J., Girek, Z., Kuzmanović, Đ., Rasulić, N.,& Jošić, D.. (2014). Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia. in Agricultural and Food Science
Scientific Agricultural Soc Finland, Univ Helsinski., 23(3), 173-185.
https://doi.org/10.23986/afsci.41385

Ugrinović M, Mijatović M, Zdravković J, Girek Z, Kuzmanović Đ, Rasulić N, Jošić D. Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia. in Agricultural and Food Science. 2014;23(3):173-185.
doi:10.23986/afsci.41385 .

Ugrinović, Milan, Mijatović, Mirjana, Zdravković, Jasmina, Girek, Zdenka, Kuzmanović, Đorđe, Rasulić, Nataša, Jošić, Dragana, "Intercropped red beet and radish with green bean affected microbial communities and nodulation by indigenous rhizobia" in Agricultural and Food Science, 23, no. 3 (2014):173-185,
https://doi.org/10.23986/afsci.41385 . .

TY  - JOUR
AU  - Dinić, Zoran
AU  - Ugrinović, Milan
AU  - Bosnić, Predrag
AU  - Mijatović, Mirjana
AU  - Zdravković, Jasmina
AU  - Miladinović, Miroslav
AU  - Jošić, Dragana
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/152
AB  - Phosphorus (P) is one of the major essential macronutrient for plant growth. Phosphate solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study endophytic bacteria were isolated form nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4. Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml-1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis.
AB  - Fosfor (P) je jedan od esencijalnih makronutrijenata potrebnih za rast biljaka. Fosfosolubilizirajuće bakterije (PCB) spadaju u grupu heterotrofnih bakterija sposobnih da solubilizuju fosfor iz nerastvornih organskih i neorganskih jedinjenja, oslobađajući fosfor koji je dostupan biljkama. U ovom radu su ispitivane endofite bakterije izolovane iz nodula boranije gajene u različitim sistemima interkropinga. Samo 14 od 158 endofitih izolata je solubilizovalo trikalcijum fosfate (TCP). Nijedan izolat nije solubilizovao FePO4 i AlPO4. Osam izolata je formiralo zone solubilizacije šire od 3 mm i selektovano je za dalji rad. TCP solubilizacija je kvantifikovana u tečnom Pikovskaya medijumu i iznosila je 14-182 μgml-1 rastvorenog fosfora. TCP solubilizacija je praćena zakišeljavanjem medijuma, a izmereno je opadanje pH sa 7,4 na 5,57 i 4,91 kod izolata sa najmanjim i najvećim solubilizacionim potencijalom. Selektovan je najefikasniji izolat MP18 i identifikovan kao Pseudomonas sp. prema izvršenoj analizi sekvence 16S rRNK gena.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules
T1  - Solubilizacija neorganskih fosfata endofitim Pseudomonas sp. iz nodula boranije
EP  - 105
IS  - 2
SP  - 100
VL  - 51
DO  - 10.5937/ratpov51-6222
ER  - 

@article{
author = "Dinić, Zoran and Ugrinović, Milan and Bosnić, Predrag and Mijatović, Mirjana and Zdravković, Jasmina and Miladinović, Miroslav and Jošić, Dragana",
year = "2014",
abstract = "Phosphorus (P) is one of the major essential macronutrient for plant growth. Phosphate solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study endophytic bacteria were isolated form nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4. Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml-1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis., Fosfor (P) je jedan od esencijalnih makronutrijenata potrebnih za rast biljaka. Fosfosolubilizirajuće bakterije (PCB) spadaju u grupu heterotrofnih bakterija sposobnih da solubilizuju fosfor iz nerastvornih organskih i neorganskih jedinjenja, oslobađajući fosfor koji je dostupan biljkama. U ovom radu su ispitivane endofite bakterije izolovane iz nodula boranije gajene u različitim sistemima interkropinga. Samo 14 od 158 endofitih izolata je solubilizovalo trikalcijum fosfate (TCP). Nijedan izolat nije solubilizovao FePO4 i AlPO4. Osam izolata je formiralo zone solubilizacije šire od 3 mm i selektovano je za dalji rad. TCP solubilizacija je kvantifikovana u tečnom Pikovskaya medijumu i iznosila je 14-182 μgml-1 rastvorenog fosfora. TCP solubilizacija je praćena zakišeljavanjem medijuma, a izmereno je opadanje pH sa 7,4 na 5,57 i 4,91 kod izolata sa najmanjim i najvećim solubilizacionim potencijalom. Selektovan je najefikasniji izolat MP18 i identifikovan kao Pseudomonas sp. prema izvršenoj analizi sekvence 16S rRNK gena.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules, Solubilizacija neorganskih fosfata endofitim Pseudomonas sp. iz nodula boranije",
pages = "105-100",
number = "2",
volume = "51",
doi = "10.5937/ratpov51-6222"
}

Dinić, Z., Ugrinović, M., Bosnić, P., Mijatović, M., Zdravković, J., Miladinović, M.,& Jošić, D.. (2014). Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 51(2), 100-105.
https://doi.org/10.5937/ratpov51-6222

Dinić Z, Ugrinović M, Bosnić P, Mijatović M, Zdravković J, Miladinović M, Jošić D. Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo. 2014;51(2):100-105.
doi:10.5937/ratpov51-6222 .

Dinić, Zoran, Ugrinović, Milan, Bosnić, Predrag, Mijatović, Mirjana, Zdravković, Jasmina, Miladinović, Miroslav, Jošić, Dragana, "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules" in Ratarstvo i povrtarstvo, 51, no. 2 (2014):100-105,
https://doi.org/10.5937/ratpov51-6222 . .