TY  - JOUR
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Šević, Milan
AU  - Pulawska, J.
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/131
AB  - Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts
VL  - 94
DO  - 10.4454/jpp.v94i1sup.020
ER  - 

@article{
author = "Prokić, Anđelka and Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Šević, Milan and Pulawska, J. and Obradović, Aleksa",
year = "2012",
abstract = "Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts",
volume = "94",
doi = "10.4454/jpp.v94i1sup.020"
}

Prokić, A., Gašić, K., Ivanović, M., Kuzmanović, N., Šević, M., Pulawska, J.,& Obradović, A.. (2012). Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts. in Journal of Plant Pathology
Springer, New York., 94.
https://doi.org/10.4454/jpp.v94i1sup.020

Prokić A, Gašić K, Ivanović M, Kuzmanović N, Šević M, Pulawska J, Obradović A. Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts. in Journal of Plant Pathology. 2012;94.
doi:10.4454/jpp.v94i1sup.020 .

Prokić, Anđelka, Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Šević, Milan, Pulawska, J., Obradović, Aleksa, "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts" in Journal of Plant Pathology, 94 (2012),
https://doi.org/10.4454/jpp.v94i1sup.020 . .