Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety

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Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (en)
Развој интегрисаних система управљања штетним организмима у биљној производњи са циљем превазилажења резистентности и унапређења квалитета и безбедности хране (sr)
Razvoj integrisanih sistema upravljanja štetnim organizmima u biljnoj proizvodnji sa ciljem prevazilaženja rezistentnosti i unapređenja kvaliteta i bezbednosti hrane (sr_RS)
Authors

Publications

Suzbijanje korova u usevima pasulja i boranije

Savić, Aleksandra; Đurović, Sanja; Stevanović, Srđan; Ugrinović, Milan

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za fitomedicinu i zaštitu životne sredine, Novi Sad, 2021)

TY  - JOUR
AU  - Savić, Aleksandra
AU  - Đurović, Sanja
AU  - Stevanović, Srđan
AU  - Ugrinović, Milan
PY  - 2021
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/411
AB  - Phaseolus vulgaris (pasulj; boranija) kao namirnica visoke nutritivne vrednosti
zauzima značajno mesto u ljudskoj ishrani. Pored svih osnovnih uslova gajenja, jedan
od ključnih zahteva za postizanje visokih prinosa je uspešna borba protiv korova.
Kao najdominantije vrste u našim agroekološkim uslovima javljaju se korovi: Amaranthus
retrofl exus, Chenopodium album, Datura stramonium, Abutilon theophrasti,
Xanthium strumarium, Cirsium arvense, Ambrosia artemisiifolia, Sorghum halapense
i dr. Primena koncepta integralnog suzbijanja korova podrazumeva primenu svih
raspoloživih mera (plodored, pravilna i pravovremena obrada zemljišta, primena herbicida
i dr.). Iako u svetu postoji širi dijapazon registrovanih herbicida za suzbijanje
korova u usevu pasulja i boranije, u Srbiji su registrovani preparati na bazi aktivnih
materija: kletodim, kvizalofop–P–tefuril, imazamoks i bentazon.
AB  - Phaseolus vulgaris (beans; green beans) as a food of high nutritional value occupies
an important place in the human diet. In addition to all the basic growing conditions,
one of the key requirements for achieving high yields is successful weed
control. Weeds such as Amaranthus retrofl exus, Chenopodium album, Datura stramonium,
Abutilon theophrasti, Xanthium strumarium, Cirsium arvense, Ambrosia
artemisiifolia, Sorghum halepense and others appear as the most dominant species
in our agroecological conditions. The application of the concept of integrated weed
control implies the application of all available measures (crop rotation, proper and
timely tillage, application of herbicides, etc.). Although there is a wider range of
registered herbicides for weed control in beans and green beans in the world, preparations
based on active substances: clethodim, quizalofop-P-tefuril, imazamox and
bentazone have been registered in Serbia.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za fitomedicinu i zaštitu životne sredine, Novi Sad
T2  - Biljni lekar / Plant doctor
T1  - Suzbijanje korova u usevima pasulja i boranije
T1  - Weed control in bean and green bean crops
IS  - 6
VL  - 49
DO  - 10.5937/BiljLek2106804S
ER  - 
@article{
author = "Savić, Aleksandra and Đurović, Sanja and Stevanović, Srđan and Ugrinović, Milan",
year = "2021",
abstract = "Phaseolus vulgaris (pasulj; boranija) kao namirnica visoke nutritivne vrednosti
zauzima značajno mesto u ljudskoj ishrani. Pored svih osnovnih uslova gajenja, jedan
od ključnih zahteva za postizanje visokih prinosa je uspešna borba protiv korova.
Kao najdominantije vrste u našim agroekološkim uslovima javljaju se korovi: Amaranthus
retrofl exus, Chenopodium album, Datura stramonium, Abutilon theophrasti,
Xanthium strumarium, Cirsium arvense, Ambrosia artemisiifolia, Sorghum halapense
i dr. Primena koncepta integralnog suzbijanja korova podrazumeva primenu svih
raspoloživih mera (plodored, pravilna i pravovremena obrada zemljišta, primena herbicida
i dr.). Iako u svetu postoji širi dijapazon registrovanih herbicida za suzbijanje
korova u usevu pasulja i boranije, u Srbiji su registrovani preparati na bazi aktivnih
materija: kletodim, kvizalofop–P–tefuril, imazamoks i bentazon., Phaseolus vulgaris (beans; green beans) as a food of high nutritional value occupies
an important place in the human diet. In addition to all the basic growing conditions,
one of the key requirements for achieving high yields is successful weed
control. Weeds such as Amaranthus retrofl exus, Chenopodium album, Datura stramonium,
Abutilon theophrasti, Xanthium strumarium, Cirsium arvense, Ambrosia
artemisiifolia, Sorghum halepense and others appear as the most dominant species
in our agroecological conditions. The application of the concept of integrated weed
control implies the application of all available measures (crop rotation, proper and
timely tillage, application of herbicides, etc.). Although there is a wider range of
registered herbicides for weed control in beans and green beans in the world, preparations
based on active substances: clethodim, quizalofop-P-tefuril, imazamox and
bentazone have been registered in Serbia.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za fitomedicinu i zaštitu životne sredine, Novi Sad",
journal = "Biljni lekar / Plant doctor",
title = "Suzbijanje korova u usevima pasulja i boranije, Weed control in bean and green bean crops",
number = "6",
volume = "49",
doi = "10.5937/BiljLek2106804S"
}
Savić, A., Đurović, S., Stevanović, S.,& Ugrinović, M.. (2021). Suzbijanje korova u usevima pasulja i boranije. in Biljni lekar / Plant doctor
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za fitomedicinu i zaštitu životne sredine, Novi Sad., 49(6).
https://doi.org/10.5937/BiljLek2106804S
Savić A, Đurović S, Stevanović S, Ugrinović M. Suzbijanje korova u usevima pasulja i boranije. in Biljni lekar / Plant doctor. 2021;49(6).
doi:10.5937/BiljLek2106804S .
Savić, Aleksandra, Đurović, Sanja, Stevanović, Srđan, Ugrinović, Milan, "Suzbijanje korova u usevima pasulja i boranije" in Biljni lekar / Plant doctor, 49, no. 6 (2021),
https://doi.org/10.5937/BiljLek2106804S . .

Tehnološki postupak zasnivanja pokrovnog useva belom slačicom (Sinapis alba L.) u organskoj ratarskoj proizvodnji

Ugrenović, Vladan; Saljnikov, Elmira; Filipović, Vladimir; Stajković Srbinović, Olivera; Ugrinović, Milan; Stanković, Slađan; Simić, Divna; Marjanović-Jeromela, Ana

(Beograd : Nacionalno udruženje za razvoj organske proizvodnje Serbia Organica, 2020)

TY  - GEN
AU  - Ugrenović, Vladan
AU  - Saljnikov, Elmira
AU  - Filipović, Vladimir
AU  - Stajković Srbinović, Olivera
AU  - Ugrinović, Milan
AU  - Stanković, Slađan
AU  - Simić, Divna
AU  - Marjanović-Jeromela, Ana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2232
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/467
AB  - Predloženo tehničko rešenje razvijano je na lokalitetu sertifikovanog organskog oglednog polja Instituta Tamiš, površine 2,5 ha na karbonatnom černozemu od 2016. do 2020. godine. Za proučavanje najpodesnijeg agrotehničkog modela za zasnivanje pokrovnog useva setvom bele slačice iskorišćena je agrotehnička situacija posle žetve ovsa (Avena sativa L.), kada je došlo do nicanja samorasta ovsa i u takvim uslovima posejana bela slačica. Početkom prve dekade avgusta u svim godinama istraživanja slačica je nikla, pa je time uspostavljen združeni pokrovni usev ovsa i bele slačice. Upravo u tome je inovativnost, jer u Srbiji ovakav pokrovni usev nije do sada zasnivan, a prednosti ovakvog rešenja su višestruke. Tehničko rešenje pripada oblasti biotehničkih nauka, naučna disciplina: ratarstvo i povrtarstvo, uža naučna disciplina: industrijsko bilje i strna žita, segment: organska proizvodnja, a odnosi se na novi tehnološki postupak zasnivanja pokrovnog useva setvom bele slačice (Sinapis alba L.) u organskoj ratarskoj proizvodnji.
AB  - This new technical solution deals with establishment of white mustard (Sinapis alba L.) cover crop in organic farming. The trial was carried out after oat (Avena sativa L.) harvest on a certified organic experimental field of Institute Tamiš in Serbia on the area of 2.5 ha on carbonate chernozem in the period 2016-2020. Such intercropping combination has not previously been reported in Serbia.
PB  - Beograd : Nacionalno udruženje za razvoj organske proizvodnje Serbia Organica
T1  - Tehnološki postupak zasnivanja pokrovnog useva belom slačicom (Sinapis alba L.) u organskoj ratarskoj proizvodnji
UR  - https://hdl.handle.net/21.15107/rcub_rivec_467
ER  - 
@misc{
author = "Ugrenović, Vladan and Saljnikov, Elmira and Filipović, Vladimir and Stajković Srbinović, Olivera and Ugrinović, Milan and Stanković, Slađan and Simić, Divna and Marjanović-Jeromela, Ana",
year = "2020",
abstract = "Predloženo tehničko rešenje razvijano je na lokalitetu sertifikovanog organskog oglednog polja Instituta Tamiš, površine 2,5 ha na karbonatnom černozemu od 2016. do 2020. godine. Za proučavanje najpodesnijeg agrotehničkog modela za zasnivanje pokrovnog useva setvom bele slačice iskorišćena je agrotehnička situacija posle žetve ovsa (Avena sativa L.), kada je došlo do nicanja samorasta ovsa i u takvim uslovima posejana bela slačica. Početkom prve dekade avgusta u svim godinama istraživanja slačica je nikla, pa je time uspostavljen združeni pokrovni usev ovsa i bele slačice. Upravo u tome je inovativnost, jer u Srbiji ovakav pokrovni usev nije do sada zasnivan, a prednosti ovakvog rešenja su višestruke. Tehničko rešenje pripada oblasti biotehničkih nauka, naučna disciplina: ratarstvo i povrtarstvo, uža naučna disciplina: industrijsko bilje i strna žita, segment: organska proizvodnja, a odnosi se na novi tehnološki postupak zasnivanja pokrovnog useva setvom bele slačice (Sinapis alba L.) u organskoj ratarskoj proizvodnji., This new technical solution deals with establishment of white mustard (Sinapis alba L.) cover crop in organic farming. The trial was carried out after oat (Avena sativa L.) harvest on a certified organic experimental field of Institute Tamiš in Serbia on the area of 2.5 ha on carbonate chernozem in the period 2016-2020. Such intercropping combination has not previously been reported in Serbia.",
publisher = "Beograd : Nacionalno udruženje za razvoj organske proizvodnje Serbia Organica",
title = "Tehnološki postupak zasnivanja pokrovnog useva belom slačicom (Sinapis alba L.) u organskoj ratarskoj proizvodnji",
url = "https://hdl.handle.net/21.15107/rcub_rivec_467"
}
Ugrenović, V., Saljnikov, E., Filipović, V., Stajković Srbinović, O., Ugrinović, M., Stanković, S., Simić, D.,& Marjanović-Jeromela, A.. (2020). Tehnološki postupak zasnivanja pokrovnog useva belom slačicom (Sinapis alba L.) u organskoj ratarskoj proizvodnji. 
Beograd : Nacionalno udruženje za razvoj organske proizvodnje Serbia Organica..
https://hdl.handle.net/21.15107/rcub_rivec_467
Ugrenović V, Saljnikov E, Filipović V, Stajković Srbinović O, Ugrinović M, Stanković S, Simić D, Marjanović-Jeromela A. Tehnološki postupak zasnivanja pokrovnog useva belom slačicom (Sinapis alba L.) u organskoj ratarskoj proizvodnji. 2020;.
https://hdl.handle.net/21.15107/rcub_rivec_467 .
Ugrenović, Vladan, Saljnikov, Elmira, Filipović, Vladimir, Stajković Srbinović, Olivera, Ugrinović, Milan, Stanković, Slađan, Simić, Divna, Marjanović-Jeromela, Ana, "Tehnološki postupak zasnivanja pokrovnog useva belom slačicom (Sinapis alba L.) u organskoj ratarskoj proizvodnji" (2020),
https://hdl.handle.net/21.15107/rcub_rivec_467 .

Integration of biological and conventional treatments in control of pepper bacterial spot

Šević, Milan; Gašić, Katarina; Ignjatov, Maja; Mijatović, Mirjana; Prokić, Anđelka; Obradović, Aleksa

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/203
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}
Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier Sci Ltd, Oxford., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006
Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .
Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .
20
5
15

Integration of biological and conventional treatments in control of pepper bacterial spot

Šević, Milan; Gašić, Katarina; Ignjatov, Maja; Mijatović, Mirjana; Prokić, Anđelka; Obradović, Aleksa

(Oxford : Elsevier Sci Ltd, 2019)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/220
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Oxford : Elsevier Sci Ltd
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Oxford : Elsevier Sci Ltd",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}
Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Oxford : Elsevier Sci Ltd., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006
Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .
Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .
20
5
15

Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot

Gašić, Katarina; Kuzmanović, Nemanja; Ivanović, Milan; Prokić, Anđelka; Šević, Milan; Obradović, Aleksa

(Frontiers Media Sa, Lausanne, 2018)

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/201
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 
@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Anđelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}
Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.02021
Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .
Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Anđelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .
5
40
18
36

Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology

Gašić, Katarina; Kuzmanović, Nemanja; Ivanović, Milan; Prokić, Anđelka; Šević, Milan; Obradović, Aleksa

(Frontiers Media Sa, Lausanne, 2018)

TY  - DATA
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/483
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
UR  - https://hdl.handle.net/21.15107/rcub_rivec_483
ER  - 
@misc{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Anđelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology",
url = "https://hdl.handle.net/21.15107/rcub_rivec_483"
}
Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne..
https://hdl.handle.net/21.15107/rcub_rivec_483
Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. in Frontiers in Microbiology. 2018;.
https://hdl.handle.net/21.15107/rcub_rivec_483 .
Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Anđelka, Šević, Milan, Obradović, Aleksa, "Supplementary data for article: Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology" in Frontiers in Microbiology (2018),
https://hdl.handle.net/21.15107/rcub_rivec_483 .

Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot

Šević, Milan; Gašić, Katarina; Đorđević, Mladen; Ignjatov, Maja; Mijatović, Mirjana; Zečević, Bogoljub; Obradović, Aleksa

(International Society for Horticultural Science, 2016)

TY  - CONF
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Đorđević, Mladen
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Zečević, Bogoljub
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/185
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes
T1  - Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot
EP  - 150
SP  - 147
VL  - 1142
DO  - 10.17660/ActaHortic.2016.1142.23
ER  - 
@conference{
author = "Šević, Milan and Gašić, Katarina and Đorđević, Mladen and Ignjatov, Maja and Mijatović, Mirjana and Zečević, Bogoljub and Obradović, Aleksa",
year = "2016",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes",
title = "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot",
pages = "150-147",
volume = "1142",
doi = "10.17660/ActaHortic.2016.1142.23"
}
Šević, M., Gašić, K., Đorđević, M., Ignjatov, M., Mijatović, M., Zečević, B.,& Obradović, A.. (2016). Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes
International Society for Horticultural Science., 1142, 147-150.
https://doi.org/10.17660/ActaHortic.2016.1142.23
Šević M, Gašić K, Đorđević M, Ignjatov M, Mijatović M, Zečević B, Obradović A. Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes. 2016;1142:147-150.
doi:10.17660/ActaHortic.2016.1142.23 .
Šević, Milan, Gašić, Katarina, Đorđević, Mladen, Ignjatov, Maja, Mijatović, Mirjana, Zečević, Bogoljub, Obradović, Aleksa, "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot" in Acta Horticulturae : VI Balkan Symposium on Vegetables and Potatoes, 1142 (2016):147-150,
https://doi.org/10.17660/ActaHortic.2016.1142.23 . .
3
1
2

Bacterial fruit blotch of watermelon in Serbia

Zlatković, Nevena; Prokić, Anđelka; Kuzmanović, Nemanja; Gašić, Katarina; Šević, Milan; Ivanović, Milan; Obradović, Aleksa

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2015)

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/171
AB  - During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014.
AB  - U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial fruit blotch of watermelon in Serbia
T1  - Bakteriozna mrljavost plodova lubenice u Srbiji
EP  - 271
IS  - 3
SP  - 265
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3880
ER  - 
@article{
author = "Zlatković, Nevena and Prokić, Anđelka and Kuzmanović, Nemanja and Gašić, Katarina and Šević, Milan and Ivanović, Milan and Obradović, Aleksa",
year = "2015",
abstract = "During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014., U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial fruit blotch of watermelon in Serbia, Bakteriozna mrljavost plodova lubenice u Srbiji",
pages = "271-265",
number = "3",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3880"
}
Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Šević, M., Ivanović, M.,& Obradović, A.. (2015). Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(3), 265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880
Zlatković N, Prokić A, Kuzmanović N, Gašić K, Šević M, Ivanović M, Obradović A. Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar. 2015;43(3):265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .
Zlatković, Nevena, Prokić, Anđelka, Kuzmanović, Nemanja, Gašić, Katarina, Šević, Milan, Ivanović, Milan, Obradović, Aleksa, "Bacterial fruit blotch of watermelon in Serbia" in Biljni lekar, 43, no. 3 (2015):265-271,
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential

Gašić, Katarina; Ivanović, Milan; Kuzmanović, Nemanja; Prokić, Anđelka; Obradović, Aleksa; Šević, Milan

(International Society for Horticultural Science, 2014)

TY  - CONF
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
AU  - Šević, Milan
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/154
AB  - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
EP  - 126
SP  - 123
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.17
ER  - 
@conference{
author = "Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Anđelka and Obradović, Aleksa and Šević, Milan",
year = "2014",
abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential",
pages = "126-123",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.17"
}
Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae
International Society for Horticultural Science., 1056, 123-126.
https://doi.org/10.17660/ActaHortic.2014.1056.17
Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae. 2014;1056:123-126.
doi:10.17660/ActaHortic.2014.1056.17 .
Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Anđelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in Acta Horticulturae, 1056 (2014):123-126,
https://doi.org/10.17660/ActaHortic.2014.1056.17 . .
2

New strategies for pepper and tomato bacterial spot control

Šević, Milan; Gašić, Katarina; Obradović, Aleksa

(Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2014)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/157
AB  - Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control.
AB  - Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - New strategies for pepper and tomato bacterial spot control
T1  - Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti
EP  - 307
IS  - 4
SP  - 296
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3603
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Obradović, Aleksa",
year = "2014",
abstract = "Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control., Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "New strategies for pepper and tomato bacterial spot control, Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti",
pages = "307-296",
number = "4",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3603"
}
Šević, M., Gašić, K.,& Obradović, A.. (2014). New strategies for pepper and tomato bacterial spot control. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 42(4), 296-307.
https://hdl.handle.net/21.15107/rcub_agrospace_3603
Šević M, Gašić K, Obradović A. New strategies for pepper and tomato bacterial spot control. in Biljni lekar. 2014;42(4):296-307.
https://hdl.handle.net/21.15107/rcub_agrospace_3603 .
Šević, Milan, Gašić, Katarina, Obradović, Aleksa, "New strategies for pepper and tomato bacterial spot control" in Biljni lekar, 42, no. 4 (2014):296-307,
https://hdl.handle.net/21.15107/rcub_agrospace_3603 .

Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro

Đorđević, Mladen; Damnjanović, Jelena; Šević, Milan; Kostić, Miroslav; Pavlović, Suzana; Marković, Tatjana; Zečević, Bogoljub

(ICNPU Bulgaria, 2013)

TY  - CONF
AU  - Đorđević, Mladen
AU  - Damnjanović, Jelena
AU  - Šević, Milan
AU  - Kostić, Miroslav
AU  - Pavlović, Suzana
AU  - Marković, Tatjana
AU  - Zečević, Bogoljub
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/574
AB  - Effectiveness of essential oils of Pinus silvestris, Petroselinum crispum, Origanum vulgare, Pimpinella anisum, Mentha piperita, Eucaliptus citriodora and Rosmarinus officinalis in control of Botrytis cinerea were tested, in vitro.
Mycelial plug of B. cinerea was transferred to the center of the Petri plate. The oils were applied as a drop onto the inner side of the plate covers on the sterile filter paper, at the concetration of 0.04, 0.06, 0.1, 0.15, 0.3 and 0.6 µl/ml of air of the plate. To enable contact of volatile phase and pathogen, the plates were kept upside down and sealed with self-adhesive foil. Inhibitory effect of tested oils has been determined four days after setting the trial by calculating percentage of inhibition of radial growth of pathogen mycelium (PIRG), while minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) have been determined seven i.e. fourteen days after. For determining MFC, plates were ventilated in the sterile laminar flow for 30 minutes in order to remove volatiles of oils. Lowest MIC and MFC expressed O.vulgre applied at 0.04 µl/ml of air, followed by P. anisum applied at 0.06 µl/ml and 0.1µl/ml, M.piperita both MIC and MFC (0.15 µl/ml), E. citriodora (0.3 µI/ml), P. crispum (0.6 µl/ml) and P.silvestris (>0.6 µl/ml of air).
Based on these results we can conclude that essential oils can be adequate solution for mntrol of B. cinerea, in vitro.
PB  - ICNPU Bulgaria
C3  - International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November
T1  - Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rivec_574
ER  - 
@conference{
author = "Đorđević, Mladen and Damnjanović, Jelena and Šević, Milan and Kostić, Miroslav and Pavlović, Suzana and Marković, Tatjana and Zečević, Bogoljub",
year = "2013",
abstract = "Effectiveness of essential oils of Pinus silvestris, Petroselinum crispum, Origanum vulgare, Pimpinella anisum, Mentha piperita, Eucaliptus citriodora and Rosmarinus officinalis in control of Botrytis cinerea were tested, in vitro.
Mycelial plug of B. cinerea was transferred to the center of the Petri plate. The oils were applied as a drop onto the inner side of the plate covers on the sterile filter paper, at the concetration of 0.04, 0.06, 0.1, 0.15, 0.3 and 0.6 µl/ml of air of the plate. To enable contact of volatile phase and pathogen, the plates were kept upside down and sealed with self-adhesive foil. Inhibitory effect of tested oils has been determined four days after setting the trial by calculating percentage of inhibition of radial growth of pathogen mycelium (PIRG), while minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) have been determined seven i.e. fourteen days after. For determining MFC, plates were ventilated in the sterile laminar flow for 30 minutes in order to remove volatiles of oils. Lowest MIC and MFC expressed O.vulgre applied at 0.04 µl/ml of air, followed by P. anisum applied at 0.06 µl/ml and 0.1µl/ml, M.piperita both MIC and MFC (0.15 µl/ml), E. citriodora (0.3 µI/ml), P. crispum (0.6 µl/ml) and P.silvestris (>0.6 µl/ml of air).
Based on these results we can conclude that essential oils can be adequate solution for mntrol of B. cinerea, in vitro.",
publisher = "ICNPU Bulgaria",
journal = "International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November",
title = "Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_rivec_574"
}
Đorđević, M., Damnjanović, J., Šević, M., Kostić, M., Pavlović, S., Marković, T.,& Zečević, B.. (2013). Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro. in International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November
ICNPU Bulgaria., 33.
https://hdl.handle.net/21.15107/rcub_rivec_574
Đorđević M, Damnjanović J, Šević M, Kostić M, Pavlović S, Marković T, Zečević B. Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro. in International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November. 2013;:33.
https://hdl.handle.net/21.15107/rcub_rivec_574 .
Đorđević, Mladen, Damnjanović, Jelena, Šević, Milan, Kostić, Miroslav, Pavlović, Suzana, Marković, Tatjana, Zečević, Bogoljub, "Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro" in International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November (2013):33,
https://hdl.handle.net/21.15107/rcub_rivec_574 .

Effectiveness of essential oils in control of Verticillium dahliae in vitro

Đorđević, Mladen; Dolovac, Nenad; Ivanović, Marija; Damnjanović, Jelena; Zečević, Bogoljub

(Institut za zaštitu bilja i životnu sredinu, Beograd, 2013)

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Dolovac, Nenad
AU  - Ivanović, Marija
AU  - Damnjanović, Jelena
AU  - Zečević, Bogoljub
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/132
AB  - Volatile phase of essential oils of Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum and Origanum vulgare were tested for their effectiveness in control of Verticillium dahliae, in vitro. Percentage of inhibition of radial growth was calculated four days after exposure of mycelia to volatiles of essential oils. Also MIC and MFC were determined as well seven i.e. fourteen days after exposure. Highest value of inhibition expressed essential oil of O. vulgare with MIC and MFC applied at 0,04μl/ml of air, followed by essential oils of P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Essential oil of E. citiodora expressed MIC and MFC applied at 0,6μl/ml of air, while essential oil of M. piperita expressed only MIC when applied at 0,3μl/ml of air. Essential oils of P. sylvestris, R. officinalis and P. crispum din not express MIC or MFC even when applied at highest rate. .
AB  - Ispitivana je efikasnost isparljive faze eteričnih ulja Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum i Origanum vulgare u inhibiciji porasta micelije patogena Verticillium dahliae u in vitro uslovima. Procenat inhibicije radijalnog porasta izračunavan je četiri dana od izlaganja micelije dejstvu para eteričnih ulja.. Takođe, izračunavana je i minimalna inhibitorna koncentracija (MIC) sedam dana nakon izlaganja kao i minimalna fungicidna koncentracija (MFC) četrnaest dana od izlaganja dejstvu para. Najveći procenat inhibicije pokazalo je ulje O. vulgare sa MIC i MFC primenjenim već u najnižoj koncentraciji 0,04μl/ml vazduha, za kojim sledi ulje P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Eterično ulje E. citriodora ostvarilo je MIC i MFC primenjeno u koncentraciji 0,6μl/ml vazduha, dok je eterično ulje M. piperita pokazalo samo MIC i to primenjeno u konc. 0,3μl/ml vazduha. Eterična ulja P. sylvestris, R. officinalis i P. crispum nisu pokazala zadovoljavajući nivo inhibicije a samim tim nisu imala ni MIC ni MFC vrednost. .
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Effectiveness of essential oils in control of Verticillium dahliae in vitro
T1  - Efikasnost eteričnih ulja u suzbijanju Verticillium dahliae patogena paradajza in vitro
EP  - 168
IS  - 3
SP  - 162
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_rivec_132
ER  - 
@article{
author = "Đorđević, Mladen and Dolovac, Nenad and Ivanović, Marija and Damnjanović, Jelena and Zečević, Bogoljub",
year = "2013",
abstract = "Volatile phase of essential oils of Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum and Origanum vulgare were tested for their effectiveness in control of Verticillium dahliae, in vitro. Percentage of inhibition of radial growth was calculated four days after exposure of mycelia to volatiles of essential oils. Also MIC and MFC were determined as well seven i.e. fourteen days after exposure. Highest value of inhibition expressed essential oil of O. vulgare with MIC and MFC applied at 0,04μl/ml of air, followed by essential oils of P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Essential oil of E. citiodora expressed MIC and MFC applied at 0,6μl/ml of air, while essential oil of M. piperita expressed only MIC when applied at 0,3μl/ml of air. Essential oils of P. sylvestris, R. officinalis and P. crispum din not express MIC or MFC even when applied at highest rate. ., Ispitivana je efikasnost isparljive faze eteričnih ulja Mentha piperita, Petroselinum crispum, Eucaliptus citriodora, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum i Origanum vulgare u inhibiciji porasta micelije patogena Verticillium dahliae u in vitro uslovima. Procenat inhibicije radijalnog porasta izračunavan je četiri dana od izlaganja micelije dejstvu para eteričnih ulja.. Takođe, izračunavana je i minimalna inhibitorna koncentracija (MIC) sedam dana nakon izlaganja kao i minimalna fungicidna koncentracija (MFC) četrnaest dana od izlaganja dejstvu para. Najveći procenat inhibicije pokazalo je ulje O. vulgare sa MIC i MFC primenjenim već u najnižoj koncentraciji 0,04μl/ml vazduha, za kojim sledi ulje P. anisum (MIC - 0,1μl/ml of air, MFC - 0,3μl/ml of air). Eterično ulje E. citriodora ostvarilo je MIC i MFC primenjeno u koncentraciji 0,6μl/ml vazduha, dok je eterično ulje M. piperita pokazalo samo MIC i to primenjeno u konc. 0,3μl/ml vazduha. Eterična ulja P. sylvestris, R. officinalis i P. crispum nisu pokazala zadovoljavajući nivo inhibicije a samim tim nisu imala ni MIC ni MFC vrednost. .",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Effectiveness of essential oils in control of Verticillium dahliae in vitro, Efikasnost eteričnih ulja u suzbijanju Verticillium dahliae patogena paradajza in vitro",
pages = "168-162",
number = "3",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_rivec_132"
}
Đorđević, M., Dolovac, N., Ivanović, M., Damnjanović, J.,& Zečević, B.. (2013). Effectiveness of essential oils in control of Verticillium dahliae in vitro. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 64(3), 162-168.
https://hdl.handle.net/21.15107/rcub_rivec_132
Đorđević M, Dolovac N, Ivanović M, Damnjanović J, Zečević B. Effectiveness of essential oils in control of Verticillium dahliae in vitro. in Zaštita bilja. 2013;64(3):162-168.
https://hdl.handle.net/21.15107/rcub_rivec_132 .
Đorđević, Mladen, Dolovac, Nenad, Ivanović, Marija, Damnjanović, Jelena, Zečević, Bogoljub, "Effectiveness of essential oils in control of Verticillium dahliae in vitro" in Zaštita bilja, 64, no. 3 (2013):162-168,
https://hdl.handle.net/21.15107/rcub_rivec_132 .

Alternative approach in control of tomato pathogen by using essential oils in vitro

Đorđević, Mladen; Đorđević, Olivera; Đorđević, Radiša; Mijatović, Mirjana; Kostić, Miroslav; Todorović, Goran; Ivanović, Mirko

(Pakistan Botanical Soc, Karachi, 2013)

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Đorđević, Olivera
AU  - Đorđević, Radiša
AU  - Mijatović, Mirjana
AU  - Kostić, Miroslav
AU  - Todorović, Goran
AU  - Ivanović, Mirko
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/147
AB  - Essential oils of Mentha piperita, Eucaliptus globulus, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum and Origanum vulgare were studied for antifungal effect of their volatile phase toward tomato pathogen (Fusarium oxysporum f.sp. lycopersici) In vitro. Antifungal effect of oils was expressed through calculating percentage of inhibition of radial growth of mycelia of pathogen, and by determining MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration). Results indicated that all of examined oils expressed antifungal activity in different concentrations. Essential oil of oregano (Origanum vulgare) was the most efficient in inhibition of mycelial growth with total inhibition applied at lowest concentration of 0.04 mu l/ml of air. MIC and MFC for this oil were also found at 0.04 mu l/ml of air. Oils of anise (Pimpinella anisum) and menthe (Mentha piperita) were also very effective. Anise oil had totally inhibited mycelium applied at 0.1 mu l/ml of air. MIC value was 0.3 l/ml of air, while MFC was higher than maximal applied concentration (>0.6 mu l/ml of air). Menthe oil had total inhibition of mycelial growth applied at 0.3 mu l/ml of air and the MIC value was at the same point. MFC was higher than maximal applied concentration (>0.6 mu l/ml of air). Other essential oils expressed high inhibition rate but it wasn't 100%. The results of this research indicate that essential oil of oregano followed by oils of menthe and anise has potential to suppress the mycelial growth Fusarium oxysporum f. sp. lycopersici, In vitro. Further research should provide answers for final goal-applying essential oils In vivo.
PB  - Pakistan Botanical Soc, Karachi
T2  - Pakistan Journal of Botany
T1  - Alternative approach in control of tomato pathogen by using essential oils in vitro
EP  - 1072
IS  - 3
SP  - 1069
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rivec_147
ER  - 
@article{
author = "Đorđević, Mladen and Đorđević, Olivera and Đorđević, Radiša and Mijatović, Mirjana and Kostić, Miroslav and Todorović, Goran and Ivanović, Mirko",
year = "2013",
abstract = "Essential oils of Mentha piperita, Eucaliptus globulus, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum and Origanum vulgare were studied for antifungal effect of their volatile phase toward tomato pathogen (Fusarium oxysporum f.sp. lycopersici) In vitro. Antifungal effect of oils was expressed through calculating percentage of inhibition of radial growth of mycelia of pathogen, and by determining MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration). Results indicated that all of examined oils expressed antifungal activity in different concentrations. Essential oil of oregano (Origanum vulgare) was the most efficient in inhibition of mycelial growth with total inhibition applied at lowest concentration of 0.04 mu l/ml of air. MIC and MFC for this oil were also found at 0.04 mu l/ml of air. Oils of anise (Pimpinella anisum) and menthe (Mentha piperita) were also very effective. Anise oil had totally inhibited mycelium applied at 0.1 mu l/ml of air. MIC value was 0.3 l/ml of air, while MFC was higher than maximal applied concentration (>0.6 mu l/ml of air). Menthe oil had total inhibition of mycelial growth applied at 0.3 mu l/ml of air and the MIC value was at the same point. MFC was higher than maximal applied concentration (>0.6 mu l/ml of air). Other essential oils expressed high inhibition rate but it wasn't 100%. The results of this research indicate that essential oil of oregano followed by oils of menthe and anise has potential to suppress the mycelial growth Fusarium oxysporum f. sp. lycopersici, In vitro. Further research should provide answers for final goal-applying essential oils In vivo.",
publisher = "Pakistan Botanical Soc, Karachi",
journal = "Pakistan Journal of Botany",
title = "Alternative approach in control of tomato pathogen by using essential oils in vitro",
pages = "1072-1069",
number = "3",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_rivec_147"
}
Đorđević, M., Đorđević, O., Đorđević, R., Mijatović, M., Kostić, M., Todorović, G.,& Ivanović, M.. (2013). Alternative approach in control of tomato pathogen by using essential oils in vitro. in Pakistan Journal of Botany
Pakistan Botanical Soc, Karachi., 45(3), 1069-1072.
https://hdl.handle.net/21.15107/rcub_rivec_147
Đorđević M, Đorđević O, Đorđević R, Mijatović M, Kostić M, Todorović G, Ivanović M. Alternative approach in control of tomato pathogen by using essential oils in vitro. in Pakistan Journal of Botany. 2013;45(3):1069-1072.
https://hdl.handle.net/21.15107/rcub_rivec_147 .
Đorđević, Mladen, Đorđević, Olivera, Đorđević, Radiša, Mijatović, Mirjana, Kostić, Miroslav, Todorović, Goran, Ivanović, Mirko, "Alternative approach in control of tomato pathogen by using essential oils in vitro" in Pakistan Journal of Botany, 45, no. 3 (2013):1069-1072,
https://hdl.handle.net/21.15107/rcub_rivec_147 .
7
9

Reaction of different tomato cultivars toward race 1 of Fusarium oxysporum f. sp. lycopersici

Đorđević, Mladen; Vatchev, Tzenko; Girek, Zdenka; Šević, Milan; Zečević, Bogoljub; Zdravković, Jasmina; Ivanović, Mirko

(Društvo genetičara Srbije, Beograd, 2012)

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Vatchev, Tzenko
AU  - Girek, Zdenka
AU  - Šević, Milan
AU  - Zečević, Bogoljub
AU  - Zdravković, Jasmina
AU  - Ivanović, Mirko
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/115
AB  - The aim of this study was to examine the reaction of different tomato cultivars towards race 1 of Fusarium oxysporum f. sp. lycopersici. The researched tomato cultivars were: Adonis, Gružanski zlatni, Jasmin crveni, Narvik SPF and SP109, breeding lines Hom-3, Hom-4, L-4, S-49, S-31, S- 35, SPA, SPR,V-100, 34/56, 93/10 and 93/16. Reaction to pathogen were tested in F1 generation, as well, in combination of 93/16 x V-100, S-35 x L- 4, S-49 x SPA, 34/56 x SPR, S-49 x Hom-4, 93/16 x Hom-3 and 93/10 x S- 31. Plants were inoculated by submerging wounded roots in to pathogen suspension. Disease was assessed 30 days after inoculation. After evaluation 15 cultivars expressed resistance toward this pathogen. Among them 93/10 x S-31, Hom-3 and 93/10 had average disease rating 1,0. Other genotypes had average disease ratings as follows: Narvik SPF and S-49 (1,1), 34/56, Hom-4 and S-49 x Hom-4 (1,2), while Adonis, 93/16 x Hom-3, 34/56 x SPR and 93/16 x V-100 had average disease rating 1,3. Cultivars SPR and 93/16 had 1,7 ADR, meanwhile Sp-109 had 1,8 average rating. Genotypes SPA (2,1), S-49 x SPA (2,1), V-100 (2,2) and L-4 (2,6) belong to the group of tolerant genotypes who did not expressed typical symptoms of chlorosis and wilt of plant but expressed some level of necrosis of xylem in lower part of plants. Most sensitive to fusarium wilt were S-35 x L-4 (3,3), S-35 (3,5), S-31 (3,6), Gružanski zlatni (3,8) and Jasmin Crveni with average disease rating of 4,0.
AB  - Cilj ovog rada je da se ispita reakcija različitih kultivara paradajza u odnosu na rasu 1 Fusarium oxysporum f. sp. lycopersici. Ispitivani genotipovi paradajza su sorte: Adonis, Gružanski zlatni, Jasmin crveni, Narvik SPF i Sp-109, selekcione linije Hom-3, Hom-4, L-4, S-49, S-31, S-35, SPA, SPR, V-100, 34/56, 93/10 i 93/16. Takođe, ispitana je i reakcija F1 generacije sledećih kombinacija 93/16 x V-100, S- 35 x L-4, S-49 x SPA, 34/56 x SPR, S-49 x Hom-4, 93/16 x Hom-3 i 93/10 x S-31. Biljke su inokulisane potapanjem povređenog korena u suspenziju patogena. Intenzitet oboljenja je ocenjivan nakon 30 dana posle inokulacije. Nakon evaluacije 15 genotipova je pokazalo rezistentnu reakciju prema ovom patogenu. Medu njima 93/10 x S-31, Hom-3 i 93/10 imali su prosečnu vrednost oboljenja (ADR) 1,0. Ostali genotipovi su bili sledećih ADR vrednosti: Narvik SPF i S-49 (1,1), 34/56, Hom-4 i S-49 x Hom-4 (1,2), dok su Adonis, 93/16 x Hom-3, 34/56 x SPR i 93/16 x V-100 imali ADR vrednost 1,3. Genotipovi SPR i 93/16 imali su 1,7 ADR, dok je Sp-109 imao 1,8 prosečnu vrednost. Genotipovi SPA (2,1), S-49 x SPA (2,1), V-100 (2,2) i L-4 (2,6) svrstani su u grupu tolerantnih genotipova koji nisu imali tipične simptome hloroze i uvenuća ali se kod njih razvio određeni nivo nekroze ksilema lokalizovane na donji deo stabla. Kao najosetljiviji prema ovom patogenu pokazali su se S-35 x L- 4 (3,3), S-35 (3,5), S-31 (3,6), Gružanski zlatni (3,8) i Jasmin Crveni sa prosečnom vrednošću oboljenja 4,0.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Reaction of different tomato cultivars toward race 1 of Fusarium oxysporum f. sp. lycopersici
T1  - Reakcija različitih kultivara paradajza prema rasi 1 Fusarium oxysporum f. sp. lycopersici
EP  - 118
IS  - 1
SP  - 109
VL  - 44
DO  - 10.2298/GENSR1201109D
ER  - 
@article{
author = "Đorđević, Mladen and Vatchev, Tzenko and Girek, Zdenka and Šević, Milan and Zečević, Bogoljub and Zdravković, Jasmina and Ivanović, Mirko",
year = "2012",
abstract = "The aim of this study was to examine the reaction of different tomato cultivars towards race 1 of Fusarium oxysporum f. sp. lycopersici. The researched tomato cultivars were: Adonis, Gružanski zlatni, Jasmin crveni, Narvik SPF and SP109, breeding lines Hom-3, Hom-4, L-4, S-49, S-31, S- 35, SPA, SPR,V-100, 34/56, 93/10 and 93/16. Reaction to pathogen were tested in F1 generation, as well, in combination of 93/16 x V-100, S-35 x L- 4, S-49 x SPA, 34/56 x SPR, S-49 x Hom-4, 93/16 x Hom-3 and 93/10 x S- 31. Plants were inoculated by submerging wounded roots in to pathogen suspension. Disease was assessed 30 days after inoculation. After evaluation 15 cultivars expressed resistance toward this pathogen. Among them 93/10 x S-31, Hom-3 and 93/10 had average disease rating 1,0. Other genotypes had average disease ratings as follows: Narvik SPF and S-49 (1,1), 34/56, Hom-4 and S-49 x Hom-4 (1,2), while Adonis, 93/16 x Hom-3, 34/56 x SPR and 93/16 x V-100 had average disease rating 1,3. Cultivars SPR and 93/16 had 1,7 ADR, meanwhile Sp-109 had 1,8 average rating. Genotypes SPA (2,1), S-49 x SPA (2,1), V-100 (2,2) and L-4 (2,6) belong to the group of tolerant genotypes who did not expressed typical symptoms of chlorosis and wilt of plant but expressed some level of necrosis of xylem in lower part of plants. Most sensitive to fusarium wilt were S-35 x L-4 (3,3), S-35 (3,5), S-31 (3,6), Gružanski zlatni (3,8) and Jasmin Crveni with average disease rating of 4,0., Cilj ovog rada je da se ispita reakcija različitih kultivara paradajza u odnosu na rasu 1 Fusarium oxysporum f. sp. lycopersici. Ispitivani genotipovi paradajza su sorte: Adonis, Gružanski zlatni, Jasmin crveni, Narvik SPF i Sp-109, selekcione linije Hom-3, Hom-4, L-4, S-49, S-31, S-35, SPA, SPR, V-100, 34/56, 93/10 i 93/16. Takođe, ispitana je i reakcija F1 generacije sledećih kombinacija 93/16 x V-100, S- 35 x L-4, S-49 x SPA, 34/56 x SPR, S-49 x Hom-4, 93/16 x Hom-3 i 93/10 x S-31. Biljke su inokulisane potapanjem povređenog korena u suspenziju patogena. Intenzitet oboljenja je ocenjivan nakon 30 dana posle inokulacije. Nakon evaluacije 15 genotipova je pokazalo rezistentnu reakciju prema ovom patogenu. Medu njima 93/10 x S-31, Hom-3 i 93/10 imali su prosečnu vrednost oboljenja (ADR) 1,0. Ostali genotipovi su bili sledećih ADR vrednosti: Narvik SPF i S-49 (1,1), 34/56, Hom-4 i S-49 x Hom-4 (1,2), dok su Adonis, 93/16 x Hom-3, 34/56 x SPR i 93/16 x V-100 imali ADR vrednost 1,3. Genotipovi SPR i 93/16 imali su 1,7 ADR, dok je Sp-109 imao 1,8 prosečnu vrednost. Genotipovi SPA (2,1), S-49 x SPA (2,1), V-100 (2,2) i L-4 (2,6) svrstani su u grupu tolerantnih genotipova koji nisu imali tipične simptome hloroze i uvenuća ali se kod njih razvio određeni nivo nekroze ksilema lokalizovane na donji deo stabla. Kao najosetljiviji prema ovom patogenu pokazali su se S-35 x L- 4 (3,3), S-35 (3,5), S-31 (3,6), Gružanski zlatni (3,8) i Jasmin Crveni sa prosečnom vrednošću oboljenja 4,0.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Reaction of different tomato cultivars toward race 1 of Fusarium oxysporum f. sp. lycopersici, Reakcija različitih kultivara paradajza prema rasi 1 Fusarium oxysporum f. sp. lycopersici",
pages = "118-109",
number = "1",
volume = "44",
doi = "10.2298/GENSR1201109D"
}
Đorđević, M., Vatchev, T., Girek, Z., Šević, M., Zečević, B., Zdravković, J.,& Ivanović, M.. (2012). Reaction of different tomato cultivars toward race 1 of Fusarium oxysporum f. sp. lycopersici. in Genetika
Društvo genetičara Srbije, Beograd., 44(1), 109-118.
https://doi.org/10.2298/GENSR1201109D
Đorđević M, Vatchev T, Girek Z, Šević M, Zečević B, Zdravković J, Ivanović M. Reaction of different tomato cultivars toward race 1 of Fusarium oxysporum f. sp. lycopersici. in Genetika. 2012;44(1):109-118.
doi:10.2298/GENSR1201109D .
Đorđević, Mladen, Vatchev, Tzenko, Girek, Zdenka, Šević, Milan, Zečević, Bogoljub, Zdravković, Jasmina, Ivanović, Mirko, "Reaction of different tomato cultivars toward race 1 of Fusarium oxysporum f. sp. lycopersici" in Genetika, 44, no. 1 (2012):109-118,
https://doi.org/10.2298/GENSR1201109D . .
8
7
11

Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars

Đorđević, Mladen; Dolovac, Nenad; Đorđević, Radiša; Trkulja, Nenad; Damnjanović, Jelena; Zdravković, Jasmina; Mijatović, Mirjana

(Institut za zaštitu bilja i životnu sredinu, Beograd, 2012)

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Dolovac, Nenad
AU  - Đorđević, Radiša
AU  - Trkulja, Nenad
AU  - Damnjanović, Jelena
AU  - Zdravković, Jasmina
AU  - Mijatović, Mirjana
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/116
AB  - Aim of this study is to determine the impact of race 3 of fusarium wilt on some tomato cultivars if it occurs in Serbia. For this purpose eleven tomato cultivars were inoculated with this pathogen: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1 by applying classic method of inoculation by submersing the injured root in fungi suspension. Disease was assessed 30 days after inoculation using an ordinal scale range from 1 - 5. After assessment, Nada F1 and 129-Sprin had lowest average disease rating (2,9), and marked as tolerant. All the other cultivars were consider susceptible with ADR values higher than 3,0 in the following order: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Based on these results we can conclude that if the race 3 of Fusarium oxysporum f. sp. lycopersici occur in Serbia it could seriously jeopardize tomato production.
AB  - Cilj ovog istraživanja je da se utvrdi kakav bi bio uticaj rase 3 fuzarioznog uvenuća paradajza na pojedine kultivare ukoliko bi se ova rasa pojavila u Srbiji. U te svrhe inokulisano je jedanaest kultivara ovim patogenom i to: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1, primenom klasične metode inokulacije umakanjem povređenog korena u suspenziju gljiva. Nakon 30 dana rađena je procena pojave oboljenja upotrebom skale od 1 - 5. Nakon evaluacije, Nada F1 i 129-Sprin su imali najnižu vrednost ADR-a (prosečni nivo oboljenja) (2,9), i obeleženi su kao tolerantni. Svi ostali kultivari smatrani su osetljivim sa vrednostima ADR višim od 3,0, po sledećem rasporedu: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Na osnovu ovih rezultata možemo zaključiti da ako bi se rasa 3 Fusarium oxysporum f. sp. lycopersici pojavila u Srbiji mogla bi značajno da ugrozi proizvodnju paradajza.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars
T1  - Uticaj rase 3 Fusarium oxysporum f.sp. lycopersici na pojedine kultivare paradajza
EP  - 28
IS  - 1
SP  - 22
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_rivec_116
ER  - 
@article{
author = "Đorđević, Mladen and Dolovac, Nenad and Đorđević, Radiša and Trkulja, Nenad and Damnjanović, Jelena and Zdravković, Jasmina and Mijatović, Mirjana",
year = "2012",
abstract = "Aim of this study is to determine the impact of race 3 of fusarium wilt on some tomato cultivars if it occurs in Serbia. For this purpose eleven tomato cultivars were inoculated with this pathogen: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1 by applying classic method of inoculation by submersing the injured root in fungi suspension. Disease was assessed 30 days after inoculation using an ordinal scale range from 1 - 5. After assessment, Nada F1 and 129-Sprin had lowest average disease rating (2,9), and marked as tolerant. All the other cultivars were consider susceptible with ADR values higher than 3,0 in the following order: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Based on these results we can conclude that if the race 3 of Fusarium oxysporum f. sp. lycopersici occur in Serbia it could seriously jeopardize tomato production., Cilj ovog istraživanja je da se utvrdi kakav bi bio uticaj rase 3 fuzarioznog uvenuća paradajza na pojedine kultivare ukoliko bi se ova rasa pojavila u Srbiji. U te svrhe inokulisano je jedanaest kultivara ovim patogenom i to: 129 - sprin, Balkan F1, Danubius F1, Jasmin crveni, M - 7, M - 10, Marko F1, Nada F1, Narvik, Šampion F1, Zlatni Jubilej F1, primenom klasične metode inokulacije umakanjem povređenog korena u suspenziju gljiva. Nakon 30 dana rađena je procena pojave oboljenja upotrebom skale od 1 - 5. Nakon evaluacije, Nada F1 i 129-Sprin su imali najnižu vrednost ADR-a (prosečni nivo oboljenja) (2,9), i obeleženi su kao tolerantni. Svi ostali kultivari smatrani su osetljivim sa vrednostima ADR višim od 3,0, po sledećem rasporedu: Narvik (3,6), Šampion F1 (3,8), M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and M-10 (4,6). Na osnovu ovih rezultata možemo zaključiti da ako bi se rasa 3 Fusarium oxysporum f. sp. lycopersici pojavila u Srbiji mogla bi značajno da ugrozi proizvodnju paradajza.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars, Uticaj rase 3 Fusarium oxysporum f.sp. lycopersici na pojedine kultivare paradajza",
pages = "28-22",
number = "1",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_rivec_116"
}
Đorđević, M., Dolovac, N., Đorđević, R., Trkulja, N., Damnjanović, J., Zdravković, J.,& Mijatović, M.. (2012). Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 63(1), 22-28.
https://hdl.handle.net/21.15107/rcub_rivec_116
Đorđević M, Dolovac N, Đorđević R, Trkulja N, Damnjanović J, Zdravković J, Mijatović M. Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars. in Zaštita bilja. 2012;63(1):22-28.
https://hdl.handle.net/21.15107/rcub_rivec_116 .
Đorđević, Mladen, Dolovac, Nenad, Đorđević, Radiša, Trkulja, Nenad, Damnjanović, Jelena, Zdravković, Jasmina, Mijatović, Mirjana, "Effect of race 3 of Fusarium oxysporum f.sp. lycopersici on some tomato cultivars" in Zaštita bilja, 63, no. 1 (2012):22-28,
https://hdl.handle.net/21.15107/rcub_rivec_116 .

Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts

Prokić, Anđelka; Gašić, Katarina; Ivanović, Milan; Kuzmanović, Nemanja; Šević, Milan; Pulawska, J.; Obradović, Aleksa

(Springer, New York, 2012)

TY  - JOUR
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Šević, Milan
AU  - Pulawska, J.
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/131
AB  - Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts
VL  - 94
DO  - 10.4454/jpp.v94i1sup.020
ER  - 
@article{
author = "Prokić, Anđelka and Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Šević, Milan and Pulawska, J. and Obradović, Aleksa",
year = "2012",
abstract = "Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts",
volume = "94",
doi = "10.4454/jpp.v94i1sup.020"
}
Prokić, A., Gašić, K., Ivanović, M., Kuzmanović, N., Šević, M., Pulawska, J.,& Obradović, A.. (2012). Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts. in Journal of Plant Pathology
Springer, New York., 94.
https://doi.org/10.4454/jpp.v94i1sup.020
Prokić A, Gašić K, Ivanović M, Kuzmanović N, Šević M, Pulawska J, Obradović A. Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts. in Journal of Plant Pathology. 2012;94.
doi:10.4454/jpp.v94i1sup.020 .
Prokić, Anđelka, Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Šević, Milan, Pulawska, J., Obradović, Aleksa, "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts" in Journal of Plant Pathology, 94 (2012),
https://doi.org/10.4454/jpp.v94i1sup.020 . .
1

Sensitivity of some pepper genotypes to bacterial spot causal agent

Ignjatov, Maja; Šević, Milan; Gašić, Katarina; Jovičić, Dušica; Nikolić, Zorica; Milošević, Dragana; Obradović, Aleksa

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2012)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/111
AB  - Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease.
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Sensitivity of some pepper genotypes to bacterial spot causal agent
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 
@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease., Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Sensitivity of some pepper genotypes to bacterial spot causal agent, Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}
Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769
Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .
Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Sensitivity of some pepper genotypes to bacterial spot causal agent" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .
4

Polyvinylpirolidon and bisublimated iodine complex as an alternative in controlling bacterial spot of pepper Xanthomonas campestris pv. Vesicatoria

Đorđević, Mladen; Damnjanović, Jelena; Zečević, Bogoljub; Đorđević, Radiša; Ugrinović, Milan

(Institut za zaštitu bilja i životnu sredinu, Beograd, 2011)

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Damnjanović, Jelena
AU  - Zečević, Bogoljub
AU  - Đorđević, Radiša
AU  - Ugrinović, Milan
PY  - 2011
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/100
AB  - The aim of this research was to test efficiency of polyvinylpirolidon and bisublimated iodine complex in controlling Xanthomonas campestris pv. vesicatoria. Efficiency of this complex has been compared to efficiency of biological pesticide Serenade, antibiotic Starner and copper pesticide Bakrocid S - 50. The trial included two treatments: (i) two hours before inoculation; (ii) four hours after inoculation and control inoculated only with the pathogen. Disease intensity (DSI) and efficiency has been observed after 14 days. The results showed that pesticide Vestal PVP-179 in treatment (i) had efficiency 77.23%, while in treatment (ii) had 72.28% which is close to the efficiency of other pesticides. The conclusion is that this pesticide could be used in controlling this pathogen.
AB  - Cilj ovog ogleda je bio da se testira efikasnost kompleksa polivinilpirolidona i bisublimiranog joda u suzbijanju Xanthomonas campestris pv. vesicatoria. Efikasnost ovog kompleksa poredila se sa efikasnošću biološkog preparata Serenade, antibiotika Starner i coppernog preparata Bakrocid S - 50. U ogledu su bila dva tretmana:(i) dva sata pre inokulacije; (ii) četiri sata posle inokulacije, i kontrolu koja je samo inokulisana patogenom. Nakon 14 dana vršena je ocena inteziteta oboljenja (DSI) i efikasnosti. Utvrđeno je da je preparat Vestal PVP-179 u tretmanu (i) imao efikasnost 77,23%, dok je u tretmanu (ii) imao 72,28% što je u granicama efikasnosti koje su imali drugi preparati. Možemo zaključiti da se ovaj preparat može koristiti u cilju suzbijanja ovog patogena.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Polyvinylpirolidon and bisublimated iodine complex as an alternative in controlling bacterial spot of pepper Xanthomonas campestris pv. Vesicatoria
T1  - Kompleks polivinilpirolidona i bisumliranog joda kao alternativa u suzbijanju bakteriozne pegavosti paprike Xanthomonas campestris pv. Vesicatoria
EP  - 24
IS  - 1
SP  - 17
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_rivec_100
ER  - 
@article{
author = "Đorđević, Mladen and Damnjanović, Jelena and Zečević, Bogoljub and Đorđević, Radiša and Ugrinović, Milan",
year = "2011",
abstract = "The aim of this research was to test efficiency of polyvinylpirolidon and bisublimated iodine complex in controlling Xanthomonas campestris pv. vesicatoria. Efficiency of this complex has been compared to efficiency of biological pesticide Serenade, antibiotic Starner and copper pesticide Bakrocid S - 50. The trial included two treatments: (i) two hours before inoculation; (ii) four hours after inoculation and control inoculated only with the pathogen. Disease intensity (DSI) and efficiency has been observed after 14 days. The results showed that pesticide Vestal PVP-179 in treatment (i) had efficiency 77.23%, while in treatment (ii) had 72.28% which is close to the efficiency of other pesticides. The conclusion is that this pesticide could be used in controlling this pathogen., Cilj ovog ogleda je bio da se testira efikasnost kompleksa polivinilpirolidona i bisublimiranog joda u suzbijanju Xanthomonas campestris pv. vesicatoria. Efikasnost ovog kompleksa poredila se sa efikasnošću biološkog preparata Serenade, antibiotika Starner i coppernog preparata Bakrocid S - 50. U ogledu su bila dva tretmana:(i) dva sata pre inokulacije; (ii) četiri sata posle inokulacije, i kontrolu koja je samo inokulisana patogenom. Nakon 14 dana vršena je ocena inteziteta oboljenja (DSI) i efikasnosti. Utvrđeno je da je preparat Vestal PVP-179 u tretmanu (i) imao efikasnost 77,23%, dok je u tretmanu (ii) imao 72,28% što je u granicama efikasnosti koje su imali drugi preparati. Možemo zaključiti da se ovaj preparat može koristiti u cilju suzbijanja ovog patogena.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Polyvinylpirolidon and bisublimated iodine complex as an alternative in controlling bacterial spot of pepper Xanthomonas campestris pv. Vesicatoria, Kompleks polivinilpirolidona i bisumliranog joda kao alternativa u suzbijanju bakteriozne pegavosti paprike Xanthomonas campestris pv. Vesicatoria",
pages = "24-17",
number = "1",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_rivec_100"
}
Đorđević, M., Damnjanović, J., Zečević, B., Đorđević, R.,& Ugrinović, M.. (2011). Polyvinylpirolidon and bisublimated iodine complex as an alternative in controlling bacterial spot of pepper Xanthomonas campestris pv. Vesicatoria. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 62(1), 17-24.
https://hdl.handle.net/21.15107/rcub_rivec_100
Đorđević M, Damnjanović J, Zečević B, Đorđević R, Ugrinović M. Polyvinylpirolidon and bisublimated iodine complex as an alternative in controlling bacterial spot of pepper Xanthomonas campestris pv. Vesicatoria. in Zaštita bilja. 2011;62(1):17-24.
https://hdl.handle.net/21.15107/rcub_rivec_100 .
Đorđević, Mladen, Damnjanović, Jelena, Zečević, Bogoljub, Đorđević, Radiša, Ugrinović, Milan, "Polyvinylpirolidon and bisublimated iodine complex as an alternative in controlling bacterial spot of pepper Xanthomonas campestris pv. Vesicatoria" in Zaštita bilja, 62, no. 1 (2011):17-24,
https://hdl.handle.net/21.15107/rcub_rivec_100 .

In vitro effectiveness of different essential oils in control of Alternaria alternata

Đorđević, Mladen; Šević, Milan; Mijatović, Mirjana; Todorović, Goran; Kostić, Miroslav

(Institut za zaštitu bilja i životnu sredinu, Beograd, 2011)

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Šević, Milan
AU  - Mijatović, Mirjana
AU  - Todorović, Goran
AU  - Kostić, Miroslav
PY  - 2011
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/102
AB  - The effectiveness of volatile phase of essential oils of menthe, eucalyptus and rosmarinus in control of A. alternata, a postharvest pathogen on fruits and vegetables, expressed through inhibition of mycelium growth, in vitro, has been tested. The inhibitory effect of tested oils has been determined four days after setting the trial by calculating the percentage of inhibition of radial growth of pathogen mycelium (PIRG), while the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) have been determined seven i.e. fourteen days later. The highest value of PIRG (100%) was found in essential oil of menthe when applied in concentration of 0,15μl/ml of air and essential oil of rosmarinus applied in 0,6μl/ ml of air. Essential oil of eucalyptus had the highest PIRG value (89,74%) when applied in concentration of 0,6μl/ml of air. MIC of essential oil of menthe was 0,3μl/ml of air while other two essential oils did not show total inhibitory effect in tested concentrations (MIC >0,6μl/ ml of air). Essential oil of menthe did not have a fungicide effect on pathogen, not even in the highest concentration (MFC > 0,6μl/ml of air). In other two oils MFC has not been determined since they did not express the inhibitory effect in the first. Therefore, menthe essential oils could be an alternative to chemicals to control A. alternata, a postharvest pathogen on fruits and vegetables, and can control this pathogen in vitro. These results will help in further testing of effectiveness of essential oils in vivo.
AB  - Ispitivana je efikasnost isparljive faze etarskih ulja mente, eukaliptusa i ruzmarina u suzbijanju A. alternata, patogena uskladištenih plodova povrtarskih i voćarskih kultura, izraženo kroz inhibiciju porasta micelije, in vitro. Inhibitorni efekat posmatranih ulja izračunavan je četiri dana nakon postavljanja ogleda i izražen je procentom inhibicije radijalnog porasta micelije patogena (PIRG) dok su minimalna inhibitorna koncentracija (MIC) i minimalna fungicidna koncentracija (MFC) izračunavane nakon sedam odnosno četrnaest dana. Etarsko ulje mente imalo je najveću vrednost PIRG (100%) pri koncentraciji ulja od 0,15μl/ ml vazduha. Stoprocentni inhibitorni efekat (PIRG) ulje ruzmarina ispoljilo je pri koncentraciji 0,6μl/ml vazduha, dok je ulje eukalipusa imalo najvišu vrednost PIRG (89,74%) pri koncentraciji 0,6μl/ml vazduha. Najnižu vrednost MIC imalo je ulje mente (0,3μl/ml vazduha) dok ulja eukaliptusa i ruzmarina nisu pokazala totalni inhibitorni efekat u ispitivanim koncentracijama (MIC > 0,6μl/ ml vazduha). Etarsko ulje mente nije pokazalo fungicidni efekat prema posmatranom patogenu ni u najvišoj koncentraciji (MFC > 0,6μl/ml vazduha). Kod druga dva ulja MFC nije ni određivana jer nisu imala ni inhibitorni efekat prema miceliji patogena. Na osnovu ovih rezultata možemo zaključiti da je primena etarskog ulja mente u cilju kontrole A. alternata, patogena uskladištenih plodova, opravdana i da se na taj način može kontrolisati ovaj patogen, in vitro. Ovi rezultati će poslužiti kao polazna tačka za dalja ispitivanja u cilju primene etarskih ulja i in vivo.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - In vitro effectiveness of different essential oils in control of Alternaria alternata
T1  - In vitro efikasnost pojedinih eteričnih ulja u suzbijanju Alternaria alternate
EP  - 168
IS  - 3
SP  - 159
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_rivec_102
ER  - 
@article{
author = "Đorđević, Mladen and Šević, Milan and Mijatović, Mirjana and Todorović, Goran and Kostić, Miroslav",
year = "2011",
abstract = "The effectiveness of volatile phase of essential oils of menthe, eucalyptus and rosmarinus in control of A. alternata, a postharvest pathogen on fruits and vegetables, expressed through inhibition of mycelium growth, in vitro, has been tested. The inhibitory effect of tested oils has been determined four days after setting the trial by calculating the percentage of inhibition of radial growth of pathogen mycelium (PIRG), while the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) have been determined seven i.e. fourteen days later. The highest value of PIRG (100%) was found in essential oil of menthe when applied in concentration of 0,15μl/ml of air and essential oil of rosmarinus applied in 0,6μl/ ml of air. Essential oil of eucalyptus had the highest PIRG value (89,74%) when applied in concentration of 0,6μl/ml of air. MIC of essential oil of menthe was 0,3μl/ml of air while other two essential oils did not show total inhibitory effect in tested concentrations (MIC >0,6μl/ ml of air). Essential oil of menthe did not have a fungicide effect on pathogen, not even in the highest concentration (MFC > 0,6μl/ml of air). In other two oils MFC has not been determined since they did not express the inhibitory effect in the first. Therefore, menthe essential oils could be an alternative to chemicals to control A. alternata, a postharvest pathogen on fruits and vegetables, and can control this pathogen in vitro. These results will help in further testing of effectiveness of essential oils in vivo., Ispitivana je efikasnost isparljive faze etarskih ulja mente, eukaliptusa i ruzmarina u suzbijanju A. alternata, patogena uskladištenih plodova povrtarskih i voćarskih kultura, izraženo kroz inhibiciju porasta micelije, in vitro. Inhibitorni efekat posmatranih ulja izračunavan je četiri dana nakon postavljanja ogleda i izražen je procentom inhibicije radijalnog porasta micelije patogena (PIRG) dok su minimalna inhibitorna koncentracija (MIC) i minimalna fungicidna koncentracija (MFC) izračunavane nakon sedam odnosno četrnaest dana. Etarsko ulje mente imalo je najveću vrednost PIRG (100%) pri koncentraciji ulja od 0,15μl/ ml vazduha. Stoprocentni inhibitorni efekat (PIRG) ulje ruzmarina ispoljilo je pri koncentraciji 0,6μl/ml vazduha, dok je ulje eukalipusa imalo najvišu vrednost PIRG (89,74%) pri koncentraciji 0,6μl/ml vazduha. Najnižu vrednost MIC imalo je ulje mente (0,3μl/ml vazduha) dok ulja eukaliptusa i ruzmarina nisu pokazala totalni inhibitorni efekat u ispitivanim koncentracijama (MIC > 0,6μl/ ml vazduha). Etarsko ulje mente nije pokazalo fungicidni efekat prema posmatranom patogenu ni u najvišoj koncentraciji (MFC > 0,6μl/ml vazduha). Kod druga dva ulja MFC nije ni određivana jer nisu imala ni inhibitorni efekat prema miceliji patogena. Na osnovu ovih rezultata možemo zaključiti da je primena etarskog ulja mente u cilju kontrole A. alternata, patogena uskladištenih plodova, opravdana i da se na taj način može kontrolisati ovaj patogen, in vitro. Ovi rezultati će poslužiti kao polazna tačka za dalja ispitivanja u cilju primene etarskih ulja i in vivo.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "In vitro effectiveness of different essential oils in control of Alternaria alternata, In vitro efikasnost pojedinih eteričnih ulja u suzbijanju Alternaria alternate",
pages = "168-159",
number = "3",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_rivec_102"
}
Đorđević, M., Šević, M., Mijatović, M., Todorović, G.,& Kostić, M.. (2011). In vitro effectiveness of different essential oils in control of Alternaria alternata. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 62(3), 159-168.
https://hdl.handle.net/21.15107/rcub_rivec_102
Đorđević M, Šević M, Mijatović M, Todorović G, Kostić M. In vitro effectiveness of different essential oils in control of Alternaria alternata. in Zaštita bilja. 2011;62(3):159-168.
https://hdl.handle.net/21.15107/rcub_rivec_102 .
Đorđević, Mladen, Šević, Milan, Mijatović, Mirjana, Todorović, Goran, Kostić, Miroslav, "In vitro effectiveness of different essential oils in control of Alternaria alternata" in Zaštita bilja, 62, no. 3 (2011):159-168,
https://hdl.handle.net/21.15107/rcub_rivec_102 .

Antagonistic effect of soil bacteria against fusarium wilt of pepper in vitro

Đorđević, Mladen; Ugrinović, Milan; Šević, Milan; Đorđević, Radiša; Mijatović, Mirjana

(Univerzitet u Kragujevcu - Agronomski fakultet, Čačak, 2011)

TY  - JOUR
AU  - Đorđević, Mladen
AU  - Ugrinović, Milan
AU  - Šević, Milan
AU  - Đorđević, Radiša
AU  - Mijatović, Mirjana
PY  - 2011
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/98
AB  - The aim of this work was to isolate bacteria from the rhizosphere of tomato and pepper plants infected with Fusarium oxysporum and from soils where the fusarium wilt pathogen was noticed earlier, as well as to explore the possibility for control of Fusarium oxysporum, the causal agent of pepper wilt, using their antagonistic traits, in vitro. A total of 25 visually different bacteria were isolated from soil and 1 was isolated from melon leaf and included in the research as it showed a strong antagonistic effect against the isolated fungus. Eleven out of 26 isolates had a more or less antagonistic effect on this pathogen. Isolate Ab23 showed the strongest inhibition rate with PIRG value (percentage of inhibition of radial growth) of 70.98%. Isolates Ab7 (61.24%), Ab17 (57.73%) and Ab1 (56.56%) also produced a satisfactory effect. Interestingly, isolate Ab9 even though not being from the soil showed a very high value of inhibition of radial growth of pathogen mycelia (59.74%). Inhibition rate of isolates Ab18, Ab21, Ab10, Ab22, Ab5, and Ab20 was 37.67% - 28.99%. Given the above, there are bacteria in the rhizosphere of diseased plants of tomato and pepper or in the soil where fusarium wilt of tomato and pepper has been previously reported that have an antagonistic effect against the causal agent of pepper wilt, Fusarium oxyporum, and that can be successfully used in control of this pathogen, in vitro. Even the isolate of non-soilborne bacteria can show antagonism against soil pathogen, in vitro.
AB  - Cilj istraživanja je bio da se izoluju bakterije iz rizosfere biljaka paradajza i paprike zaraženih patogenom Fusarium oxysporum ali i iz zemljišta gde je ovaj patogen prisutan od ranije, kao i da se ispita mogućnost kontrole patogena prouzrokovača fuzarioznog uvenuća paprike, korišćenjem njihovih antagonističkih osobina, in vitro. U istraživanju je korišćeno ukupno 25 vizuelno različitih bakterija izolovanih iz zemljišta kao i jedna bakterija izolovana sa lista dinje koja je uključena u istraživanje zbog zapaženih jakih antagonističkih svojstava prema gljivama. Jedanaest izolata od 26 je pokazalo manje ili jače izražen antagonistički efekat prema ovom patogenu. Izolat Ab23 je pokazao najjaču inhibiciju porasta sa vrednosti PIRG-a (procenat inhibicije radijalnog porasta) 70,98%. Izolati Ab7 (61,24%), Ab17 (57,73%) i Ab1 (56,56%) su takođe imali zadovoljavajući efekat. Interesantno je da je izolat Ab9, iako nije poreklom zemljišni, imao vrlo visok nivo inhibicije radijalnog porasta micelije patogena (59,74%). Procenat inhibicije izolata Ab18, Ab21, Ab10, Ab22, Ab5, i Ab20 se kretao između 37,67% i 28,99%. Uzimajući sve u obzir možemo zaključiti da postoje bakterije u rizosferi obolelih biljaka paradajza i paprika ili u zemljištu gde je patogen fuzarioznog uvenuća ovih kultura ranije zabeležen, koje imaju antagonističkog efekta prema prouzrokovaču fuzarioznog uvenuća paprike Fusasrium oxysporum, i da se one mogu sa uspehom primeniti u kontroli ovog patogena, in vitro. Čak i izolat bakterije koja nije zemljišna može imati antagonistički efekat prema zemljišnom patogenu, in vitro.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Antagonistic effect of soil bacteria against fusarium wilt of pepper in vitro
T1  - Antagonistički efekat zemljišnih bakterija prema prouzrokovaču fuzarioznog uvenuća paprika in vitro
EP  - 31
IS  - 31
SP  - 19
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_rivec_98
ER  - 
@article{
author = "Đorđević, Mladen and Ugrinović, Milan and Šević, Milan and Đorđević, Radiša and Mijatović, Mirjana",
year = "2011",
abstract = "The aim of this work was to isolate bacteria from the rhizosphere of tomato and pepper plants infected with Fusarium oxysporum and from soils where the fusarium wilt pathogen was noticed earlier, as well as to explore the possibility for control of Fusarium oxysporum, the causal agent of pepper wilt, using their antagonistic traits, in vitro. A total of 25 visually different bacteria were isolated from soil and 1 was isolated from melon leaf and included in the research as it showed a strong antagonistic effect against the isolated fungus. Eleven out of 26 isolates had a more or less antagonistic effect on this pathogen. Isolate Ab23 showed the strongest inhibition rate with PIRG value (percentage of inhibition of radial growth) of 70.98%. Isolates Ab7 (61.24%), Ab17 (57.73%) and Ab1 (56.56%) also produced a satisfactory effect. Interestingly, isolate Ab9 even though not being from the soil showed a very high value of inhibition of radial growth of pathogen mycelia (59.74%). Inhibition rate of isolates Ab18, Ab21, Ab10, Ab22, Ab5, and Ab20 was 37.67% - 28.99%. Given the above, there are bacteria in the rhizosphere of diseased plants of tomato and pepper or in the soil where fusarium wilt of tomato and pepper has been previously reported that have an antagonistic effect against the causal agent of pepper wilt, Fusarium oxyporum, and that can be successfully used in control of this pathogen, in vitro. Even the isolate of non-soilborne bacteria can show antagonism against soil pathogen, in vitro., Cilj istraživanja je bio da se izoluju bakterije iz rizosfere biljaka paradajza i paprike zaraženih patogenom Fusarium oxysporum ali i iz zemljišta gde je ovaj patogen prisutan od ranije, kao i da se ispita mogućnost kontrole patogena prouzrokovača fuzarioznog uvenuća paprike, korišćenjem njihovih antagonističkih osobina, in vitro. U istraživanju je korišćeno ukupno 25 vizuelno različitih bakterija izolovanih iz zemljišta kao i jedna bakterija izolovana sa lista dinje koja je uključena u istraživanje zbog zapaženih jakih antagonističkih svojstava prema gljivama. Jedanaest izolata od 26 je pokazalo manje ili jače izražen antagonistički efekat prema ovom patogenu. Izolat Ab23 je pokazao najjaču inhibiciju porasta sa vrednosti PIRG-a (procenat inhibicije radijalnog porasta) 70,98%. Izolati Ab7 (61,24%), Ab17 (57,73%) i Ab1 (56,56%) su takođe imali zadovoljavajući efekat. Interesantno je da je izolat Ab9, iako nije poreklom zemljišni, imao vrlo visok nivo inhibicije radijalnog porasta micelije patogena (59,74%). Procenat inhibicije izolata Ab18, Ab21, Ab10, Ab22, Ab5, i Ab20 se kretao između 37,67% i 28,99%. Uzimajući sve u obzir možemo zaključiti da postoje bakterije u rizosferi obolelih biljaka paradajza i paprika ili u zemljištu gde je patogen fuzarioznog uvenuća ovih kultura ranije zabeležen, koje imaju antagonističkog efekta prema prouzrokovaču fuzarioznog uvenuća paprike Fusasrium oxysporum, i da se one mogu sa uspehom primeniti u kontroli ovog patogena, in vitro. Čak i izolat bakterije koja nije zemljišna može imati antagonistički efekat prema zemljišnom patogenu, in vitro.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Antagonistic effect of soil bacteria against fusarium wilt of pepper in vitro, Antagonistički efekat zemljišnih bakterija prema prouzrokovaču fuzarioznog uvenuća paprika in vitro",
pages = "31-19",
number = "31",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_rivec_98"
}
Đorđević, M., Ugrinović, M., Šević, M., Đorđević, R.,& Mijatović, M.. (2011). Antagonistic effect of soil bacteria against fusarium wilt of pepper in vitro. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 16(31), 19-31.
https://hdl.handle.net/21.15107/rcub_rivec_98
Đorđević M, Ugrinović M, Šević M, Đorđević R, Mijatović M. Antagonistic effect of soil bacteria against fusarium wilt of pepper in vitro. in Acta agriculturae Serbica. 2011;16(31):19-31.
https://hdl.handle.net/21.15107/rcub_rivec_98 .
Đorđević, Mladen, Ugrinović, Milan, Šević, Milan, Đorđević, Radiša, Mijatović, Mirjana, "Antagonistic effect of soil bacteria against fusarium wilt of pepper in vitro" in Acta agriculturae Serbica, 16, no. 31 (2011):19-31,
https://hdl.handle.net/21.15107/rcub_rivec_98 .