Приказ основних података о документу

In vitro kultura kao deo procesa oplemenjivanja Brassica oleracea var. capitata L.

dc.creatorPavlović, Suzana
dc.creatorAdžić, Slađan
dc.creatorCvikić, Dejan
dc.creatorZdravković, Jasmina
dc.creatorZdravković, Milan
dc.date.accessioned2021-06-07T12:20:59Z
dc.date.available2021-06-07T12:20:59Z
dc.date.issued2012
dc.identifier.issn0534-0012
dc.identifier.urihttp://RIVeC.institut-palanka.rs/handle/123456789/113
dc.description.abstractFourteen genotypes of cabbage (Brassica oleracea var. capitata L.), that are a part of Institute for Vegetable Crops collection, were tested for their ability to regenerate shoots in vitro. Five of them are early, while nine are late genotypes. Lateral buds from plants grown in the open field were used as explants. In all genotypes, lateral buds showed the high percentage of shoot formation, ranged from 80% to 100%. They were incubated on Murashige and Skoog’s (MS) media supplemented with 1.0 and 2.0 mgl-1 of benzyladenine (BA) or 1.0 mgl-1 6-furfurylaminopurine (KIN) in combination with 0, 0.5 and 1.0 mgl-1 indole-3-butyric acid (IBA). The BA- supplemented media were optimal for both growth and multiplication of shoots. In both groups of genotypes, the highest index of multiplication (IM) was achieved on medium supplemented with 2.0 mgl-1 BA and 1.0 mgl-1 IBA, in R9 early genotype (IM 8.53) and K1 late genotype (IM 10.06). R5 early and in K29 and K75 late genotypes had no multiplication on medium with 1.0 mgl-1 KIN (IM 1.00). Also, in all genotypes the lowest index of multiplication was observed on media supplemented with KIN (without or in combination with IBA).en
dc.description.abstractKod četrnaest genotipova kupusa (Brassica oleracea var. capitata L.), koji su deo kolekcije Instituta za povrtarstvo, ispitana je sposobnost in vitro regeneracije pupoljaka. Ranih kupusa je bilo pet gonotipova, dok je kasnih kupusa bilo devet genotipova. Bočni pupoljci biljaka gajenih na otvorenom polju korišćeni su kao eksplantati. Kod svih genotipova bočni pupoljci su pokazali visok procenat regeneracije pupoljaka, od 80% do 100%. Inkubirani su na Murashige and Skoog’s (MS) hranljivoj podlozi sa dodatkom 1.0 i 2.0 mgl-1 benziladenina (BA) ili 1.0 mgl-1 6-furfurilaminopurina (KIN) u kombinacija 0, 0.5 i 1.0 mgl-1 indole-3-butirične kiseline (IBA). Podloge koje su sadržale BA su bile optimalne, kako za regeneraciju pupoljaka, tako i za njihovu kasniju multiplikaciju. Kod obe grupe genotipova najveći indeks multiplikacije je postignut na podlozi sa dodatkom 2.0 mgl-1 BA i 1.0 mgl-1 IBA, kod R9 ranog genotipa (IM 8.53) i kod K1 kasnog genotipa (IM 10.06). Kod R5 ranog genotipa i kod K29 i K75 kasnih genotipova nije bilo multiplikacije na podlozi sa 1.0 mgl-1 KIN (IM 1.00). Takođe najmanji indeks multiplikacije kod svih genotipova je primećen na podlogama sa KIN (bez ili u kombinaciji sa IBA).sr
dc.publisherDruštvo genetičara Srbije, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31059/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceGenetika
dc.subjectcabbageen
dc.subjectmicropropagationen
dc.subjectlateral budsen
dc.subjectmultiplication indexen
dc.titleIn vitro culture as a part of Brassica oleracea var. capitata L. breedingen
dc.titleIn vitro kultura kao deo procesa oplemenjivanja Brassica oleracea var. capitata L.sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage618
dc.citation.issue3
dc.citation.other44(3): 611-618
dc.citation.rankM23
dc.citation.spage611
dc.citation.volume44
dc.description.other[http://www.dgsgenetika.org.rs/abstrakti/vol44no3_rad16.pdf]
dc.identifier.doi10.2298/GENSR1203611P
dc.identifier.fulltexthttp://RIVeC.institut-palanka.rs/bitstream/id/10/110.pdf
dc.identifier.scopus2-s2.0-84873454479
dc.identifier.wos000314459900016
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу