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Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.

Authorized Users Only
2013
Authors
Pavlović, Suzana
Adžić, Slađan
Zdravković, Jasmina
Cvikić, Dejan
Milojević, Jelena
Vinterhalter, Branka
Mitić, Nevena
Conference object (Published version)
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Abstract
An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per e...xplant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.

Keywords:
Savoy cabbage / micropropagation / immature zygotic embryos / somatic embryogenesis / secondary somatic embryos
Source:
Acta Horticulturae, 2013, 1005, 239-246
Publisher:
  • International Society for Horticultural Science
Funding / projects:
  • Integrating biotechnology approach in breeding vegetable crops for sustainable agricultural systems (RS-31059)
  • Diversity of the flora and vegetation of the Central Balkans: Ecology, chorology, and conservation (RS-143015)
Note:
  • https://www.ishs.org/ishs-article/1005_26

DOI: 10.17660/ActaHortic.2013.1005.26

ISBN: 978-90-66055-85-8

ISSN: 0567-7572

WoS: 000333261700026

Scopus: 2-s2.0-84886996923
[ Google Scholar ]
URI
http://RIVeC.institut-palanka.rs/handle/123456789/139
Collections
  • Radovi istraživača 1 / Researcher's papers 1
Institution/Community
Institut za povrtarstvo
TY  - CONF
AU  - Pavlović, Suzana
AU  - Adžić, Slađan
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Mitić, Nevena
PY  - 2013
UR  - http://RIVeC.institut-palanka.rs/handle/123456789/139
AB  - An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.
EP  - 246
SP  - 239
VL  - 1005
DO  - 10.17660/ActaHortic.2013.1005.26
ER  - 
@conference{
author = "Pavlović, Suzana and Adžić, Slađan and Zdravković, Jasmina and Cvikić, Dejan and Milojević, Jelena and Vinterhalter, Branka and Mitić, Nevena",
year = "2013",
abstract = "An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.",
pages = "246-239",
volume = "1005",
doi = "10.17660/ActaHortic.2013.1005.26"
}
Pavlović, S., Adžić, S., Zdravković, J., Cvikić, D., Milojević, J., Vinterhalter, B.,& Mitić, N.. (2013). Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae
International Society for Horticultural Science., 1005, 239-246.
https://doi.org/10.17660/ActaHortic.2013.1005.26
Pavlović S, Adžić S, Zdravković J, Cvikić D, Milojević J, Vinterhalter B, Mitić N. Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae. 2013;1005:239-246.
doi:10.17660/ActaHortic.2013.1005.26 .
Pavlović, Suzana, Adžić, Slađan, Zdravković, Jasmina, Cvikić, Dejan, Milojević, Jelena, Vinterhalter, Branka, Mitić, Nevena, "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L." in Acta Horticulturae, 1005 (2013):239-246,
https://doi.org/10.17660/ActaHortic.2013.1005.26 . .

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