Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.
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2013
Authors
Pavlović, Suzana
Adžić, Slađan

Zdravković, Jasmina

Cvikić, Dejan

Milojević, Jelena

Vinterhalter, Branka

Mitić, Nevena

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An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per e...xplant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.
Keywords:
Savoy cabbage / micropropagation / immature zygotic embryos / somatic embryogenesis / secondary somatic embryosSource:
Acta Horticulturae, 2013, 1005, 239-246Publisher:
- International Society for Horticultural Science
Funding / projects:
- Integrating biotechnology approach in breeding vegetable crops for sustainable agricultural systems (RS-31059)
- Diversity of the flora and vegetation of the Central Balkans: Ecology, chorology, and conservation (RS-143015)
DOI: 10.17660/ActaHortic.2013.1005.26
ISBN: 978-90-66055-85-8
ISSN: 0567-7572
WoS: 000333261700026
Scopus: 2-s2.0-84886996923
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Institut za povrtarstvoTY - CONF AU - Pavlović, Suzana AU - Adžić, Slađan AU - Zdravković, Jasmina AU - Cvikić, Dejan AU - Milojević, Jelena AU - Vinterhalter, Branka AU - Mitić, Nevena PY - 2013 UR - http://RIVeC.institut-palanka.rs/handle/123456789/139 AB - An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage. PB - International Society for Horticultural Science C3 - Acta Horticulturae T1 - Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L. EP - 246 SP - 239 VL - 1005 DO - 10.17660/ActaHortic.2013.1005.26 ER -
@conference{ author = "Pavlović, Suzana and Adžić, Slađan and Zdravković, Jasmina and Cvikić, Dejan and Milojević, Jelena and Vinterhalter, Branka and Mitić, Nevena", year = "2013", abstract = "An efficient plant propagation system through somatic embryogenesis by using immature zygotic embryos was established in Savoy cabbage (Brassica oleracea var. sabauda L.). The effects of zygotic embryo age, presence of 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) (B5-D) in the culture medium, and the pH (5.0 and 5.8) of the B5 plant growth regulator (PGR)-free (B5-0) induction media were investigated. It appeared that the developmental stage of the immature zygotic embryos used as explants was the most important factor for somatic embryogenesis. Highest frequency of somatic embryogenesis (86.67%) was achieved directly on zygotic embryos at cotyledonary stage (1.8 mm long) of development that cultured on B5-0 medium. Optimal pH of the medium was 5.0. Mean number of primary somatic embryos per explant on B5-0 pH 5.0 medium was 7.2. The inclusion of 2,4-D into the induction medium significantly reduced both frequency of somatic embryogenesis (to 53.34 %) and mean number of embryos per explant (to 2.62) in cotyledonary zygotic embryos. After transferring to PGR-free Murashige and Skoog (MS-0) medium 69.69% of the primary somatic embryos of the Savoy cabbage produced secondary somatic embryos (SSEs) Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of Savoy cabbage.", publisher = "International Society for Horticultural Science", journal = "Acta Horticulturae", title = "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.", pages = "246-239", volume = "1005", doi = "10.17660/ActaHortic.2013.1005.26" }
Pavlović, S., Adžić, S., Zdravković, J., Cvikić, D., Milojević, J., Vinterhalter, B.,& Mitić, N.. (2013). Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae International Society for Horticultural Science., 1005, 239-246. https://doi.org/10.17660/ActaHortic.2013.1005.26
Pavlović S, Adžić S, Zdravković J, Cvikić D, Milojević J, Vinterhalter B, Mitić N. Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L.. in Acta Horticulturae. 2013;1005:239-246. doi:10.17660/ActaHortic.2013.1005.26 .
Pavlović, Suzana, Adžić, Slađan, Zdravković, Jasmina, Cvikić, Dejan, Milojević, Jelena, Vinterhalter, Branka, Mitić, Nevena, "Induction of Direct Somatic Embryogenesis from Immature Embryos of Brassica oleracea var. Sabauda L." in Acta Horticulturae, 1005 (2013):239-246, https://doi.org/10.17660/ActaHortic.2013.1005.26 . .