Use of different types of extracts as biostimulators in organic agriculture

Abstract

This paper presents the results of the analysis of extracts obtained from: Thymus serpyllum L., Urtica dioica L., Equisetum arvense L. and Achillea millefolium L. Extracts were subjected to the following analyses: determination of total phenols, total flavonoids and anthocyanins, and refractometric determination of soluble solids. The results showed that the content of flavonoids was 0.4710 mg/ml in Urtica dioicа extract, 0.3950 mg/ml in Equisetum arvense extract, 0.2997 mg/ml in Thymus serpyllum extract and 0.1556 mg/ml in Achillea millefolium extract. The highest content of phenolic compounds was found in Thymus serpyllum extract (2.3583 mg/ml), followed by Equisetum arvense (2.2724 mg/ml) and Urtica dioicа extract (2.1374 mg/ml), and the lowest in Achillea millefolium (1.8061 mg/ml). The content of anthocyanins was highest in Urtica dioicа extract (3.966mg/l), followed by Thymus serpyllum (3.715mg/l) and Equisetum arvense (3.549mg/l) extracts, and lowest in Achillea millefolium extract (0.0835mg/l). The soluble solids content was highest in Urtica dioicа and Equisetum arvense (2 BRIX% at 20o C), followed by Thymus serpyllum (1.5 BRIX% at 20o C), and lowest in Achillea millefolium (1 BRIX% at 20o C). The results showed that extracts obtained by maceration contained bioactive components; this study, therefore, provides a basis for further and deeper research on the use of these extracts as potential biostimulators in organic agriculture.

Ovaj rad prezentuje rezulate analize ekstrakata dobijenih od: Thymus serpyllum L., Urtica dioica L., Equisetum arvense L. i Achillea millefolium L. Na ekstraktima su rađene sledeće analize: određivanje ukupnih fenola, flavonoida i antocijana, kao i refraktometrijsko određivanje rastvorljivih materija. Rezultati su pokazali da je koncentracija flavonoida bila 0.4710 mg/ml in ekstraktu Urtica dioicа, 0.3950 mg/ml u ekstraktu Equisetum arvense, 0.2997 mg/ml in ekstraktu Thymus serpyllum i 0.1556 mg/ml u ekstraktu Achillea millefolium. Najveći sadržaj fenolnih komponenti je pronađen u ekstraktu Thymus serpyllum (2.3583 mg/ml), zatim u ekstraktima Equisetum arvense (2.2724 mg/ml) i Urtica dioicа (2.1374 mg/ml), a najniži u Achillea millefolium (1.8061 mg/ml). Najveći sadržaj antocijana je pranađen u ekstraktu Urtica dioicа (3.966mg/l), zatim u ekstraktima Thymus serpyllum (3.715mg/l) i Equisetum arvense (3.549mg/l), a najniži u ekstarktu Achillea millefolium (0.0835mg/l). Najveći sadržaj rastvorljivih materija je zabeležen u Urtica dioicа i Equisetum arvense (2 BRIX% na 20o C), zatim u Thymus serpyllum (1.5 BRIX% na 20o C), a najniži kod Achillea millefolium (1 BRIX% na 20o C). Rezultati su pokazali da ektsrakti dobijeni maceracijom sadže bioaktivne komponente, tako da ovaj rad pruža osnovu za dalje i dublje istraživanje upotrebe ovih ekstrakata kao potencijalnih biostimulatora u organskoj poljoprivredi.