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dc.creatorĐurić, Nenad
dc.creatorCvijanović, Gorica
dc.creatorMatković, Mirela
dc.creatorDozet, Gordana
dc.creatorBranković, Gordana
dc.date.accessioned2021-08-11T12:19:01Z
dc.date.available2021-08-11T12:19:01Z
dc.date.issued2015
dc.identifier.issn0002-1954
dc.identifier.urihttp://RIVeC.institut-palanka.rs/handle/123456789/324
dc.description.abstractOdržanje i kontrola genetske čistoće, odnosno genetskog identiteta, jedan je od krajnjih ciljeva sjemenarstva nakon priznavanja neke sorte pšenice. Primjena pozitivne i negativne selekcije, na razini fenotipa pri sortnoj reprodukciji pšenice, omogućila je sortnu čistoću od 99 i 100% u usjevima svih sjemenskih kategorija. U cilju ispitivanja genetske identičnosti, u ovom su radu analizirane rezervne bjelačevine pšenice – glijadini, koji su metodom poliakrilne gel elektroforeze razdvojeni na veliki broj frakcija koje pokazuju visoku heterogenost. Od analiziranih sorti pšenice jedino je sorta PKB Rodika imala genetsku čistoću od 100% za sve kategorije sjemena. Sorta PKB Kristina je imala genetsku čistoću od 99% za kategoriju predosnovno sjeme, a sorta BG Merkur 99% genetske čistoće za kategoriju – sjeme prve generacije C1. Elektroforeza bjelančevina pokazala je da postoji divergentnost između ispitivanih sorti pšenice po sastavu komponenti glijadinske frakcije, dok unutar sorti postoji ujednačenost.sr
dc.description.abstractMaintenance and control of genetic purity and genetic identity is one of the ultimate goals in seed production after the recognition of some varieties of wheat. The application of positive and negative selection at the phenotypic level in varietal reproduction of wheat, enabled 99 and 100% of varietal purity in crops of all seed categories. In order to study the genetic identity, in this paper the reserve proteins of wheat – gliadins were analyzed. They were separated by polyacrylic gel electrophoresis into numerous components that showed great heterogeneity. Of all the analyzed wheat varieties, only PKB Rodika variety had the genetic purity of 100% in all categories of seed. PKB Kristina had the genetic purity of 99% in the pre-basic seed category and the variety BG Merkur had genetic purity of 99% in the seed of first generation – C1. Protein electrophoresis showed there was the divergence among the varieties of wheat in its composition of gliadin fractions, while there was uniformity among defferent varietal reproductions within each variety.sr
dc.language.isoensr
dc.language.isoothersr
dc.publisherZagreb : Hrvatsko agronomsko društvosr
dc.rightsopenAccesssr
dc.rights.urihttps://creativecommons.org/licenses/by-nd/4.0/
dc.sourceAgronomski glasniksr
dc.subjectpšenicasr
dc.subjectgenetski identitetsr
dc.subjectelektroforeza proteinasr
dc.subjectwheatsr
dc.subjectgenetic identitysr
dc.subjectprotein electrophoresissr
dc.titleControl of genetic identity by protein electrophoresis in varietal reproduction of wheatsr
dc.typearticlesr
dc.rights.licenseBY-NDsr
dc.citation.epage183
dc.citation.issue4-6
dc.citation.spage173
dc.citation.volume77
dc.identifier.fulltexthttp://RIVeC.institut-palanka.rs/bitstream/id/750/2_Duric_005.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_rivec_324
dc.type.versionpublishedVersionsr


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