Comparison of amino acids contents in barley and wheat

Abstract

Variability of amino acids composition of 6 barley and 6 wheat divergent genotypes were identified by using method of chromatography. Concentration of identified amino acids was established by method of spectrophotometry. Total concentration of free amino acids was determined by standard curved line for tyrosine while concentration for each amino acid was determined by using of standard curved line for glycine. Qualitative and quantitative analysis of amino acids showed that glutamic acid, proline, threonine, norvaline, tryptophan, were the most present in seeds of analyzed cereal species cultivars. Quantity of free amino acid in wheat varies in the ratio from 35 to 92 mg/ml and in barley from 41 to 81 mg/ml. Concentration of glutamic acid in wheat was 6.5 mg/ml and was higher than contents of other amino acids. In barley cultivars the highest concentration was established for amino acids proline (12.5 mg/ml)

Varijabilnost sadržaja aminokiselina kod 6 sorti ječma i 6 divergentnih genotipova pšenice je identifikovana korišćenjem metode hromatogragije. Koncentracija amino kiselina je ustanovljena metodom spektrofotometrije. Ukupna koncentracija slobodnih aminokiselina je odredjivana pomoću standardne krive linije za tirozin, dok je koncentracija pojedinih aminokiselina odredjivana korišćenjemstandardne krive linije za glicin. Kvalitativna i kvantitativna anliza aminokiselina je pokazala da su u semenu ječma i pšenice od slobodnih aminokiselina najzastupljenije: glutaminska kiselina, prolin, treonin, norvalin, triptofan. Kvantitet aminokiselina kod pšenice varira u rasponu od 35-92 mg/ml a kod ispitivanih sorti ječma od 41 do 81 mg/ml. Koncentracija glutaminske kiseline kod pšenice bila je najveća 6.5 mg/ml u poredjenju sa drugim aminokiselinama. Kod sorti ječma najveća koncetracija je ustanovljena za aminokiselinu prolin (12.5 mg/ml).