Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.
Keywords:
Agrobacterium / bar gene / Brassica oleracea var. sabauda / transformation
Source:
Biologia Plantarum, 2004, 48, 3, 431-436
Publisher:
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
TY - JOUR
AU - Sretenović-Rajičić, Tatjana
AU - Ninković, S.
AU - Vinterhalter, Branka
AU - Miljuš-Đukić, J
AU - Vinterhalter, Dragan
PY - 2004
UR - http://RIVeC.institut-palanka.rs/handle/123456789/55
AB - Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.
PB - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2 - Biologia Plantarum
T1 - Introduction of resistance to herbicide Basta (R) in Savoy cabbage
EP - 436
IS - 3
SP - 431
VL - 48
DO - 10.1023/B:BIOP.0000041098.18836.be
ER -
@article{
author = "Sretenović-Rajičić, Tatjana and Ninković, S. and Vinterhalter, Branka and Miljuš-Đukić, J and Vinterhalter, Dragan",
year = "2004",
abstract = "Resistance to herbicide Basta(R) was introduced into pure inbred lines of Savoy cabbage ( Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm(-3) 6-benzyladenine and 0.5 mg dm(-3) indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm(-3) phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta(R)-resistance by spraying with 15 - 60 mg dm(-3) L-PPT. Surviving plants were selfed and Basta(R)- resistance was demonstrated in T-1 progeny.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Introduction of resistance to herbicide Basta (R) in Savoy cabbage",
pages = "436-431",
number = "3",
volume = "48",
doi = "10.1023/B:BIOP.0000041098.18836.be"
}
Sretenović-Rajičić, T., Ninković, S., Vinterhalter, B., Miljuš-Đukić, J.,& Vinterhalter, D.. (2004). Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 48(3), 431-436.
https://doi.org/10.1023/B:BIOP.0000041098.18836.be
Sretenović-Rajičić T, Ninković S, Vinterhalter B, Miljuš-Đukić J, Vinterhalter D. Introduction of resistance to herbicide Basta (R) in Savoy cabbage. in Biologia Plantarum. 2004;48(3):431-436.
doi:10.1023/B:BIOP.0000041098.18836.be .
Sretenović-Rajičić, Tatjana, Ninković, S., Vinterhalter, Branka, Miljuš-Đukić, J, Vinterhalter, Dragan, "Introduction of resistance to herbicide Basta (R) in Savoy cabbage" in Biologia Plantarum, 48, no. 3 (2004):431-436,
https://doi.org/10.1023/B:BIOP.0000041098.18836.be . .
