Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro
Само за регистроване кориснике
2013
Аутори
Đorđević, MladenDamnjanović, Jelena
Šević, Milan
Kostić, Miroslav
Pavlović, Suzana
Marković, Tatjana
Zečević, Bogoljub
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Effectiveness of essential oils of Pinus silvestris, Petroselinum crispum, Origanum vulgare, Pimpinella anisum, Mentha piperita, Eucaliptus citriodora and Rosmarinus officinalis in control of Botrytis cinerea were tested, in vitro.
Mycelial plug of B. cinerea was transferred to the center of the Petri plate. The oils were applied as a drop onto the inner side of the plate covers on the sterile filter paper, at the concetration of 0.04, 0.06, 0.1, 0.15, 0.3 and 0.6 µl/ml of air of the plate. To enable contact of volatile phase and pathogen, the plates were kept upside down and sealed with self-adhesive foil. Inhibitory effect of tested oils has been determined four days after setting the trial by calculating percentage of inhibition of radial growth of pathogen mycelium (PIRG), while minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) have been determined seven i.e. fourteen days after. For determining MFC, plates were ventilated in the sterile laminar flo...w for 30 minutes in order to remove volatiles of oils. Lowest MIC and MFC expressed O.vulgre applied at 0.04 µl/ml of air, followed by P. anisum applied at 0.06 µl/ml and 0.1µl/ml, M.piperita both MIC and MFC (0.15 µl/ml), E. citriodora (0.3 µI/ml), P. crispum (0.6 µl/ml) and P.silvestris (>0.6 µl/ml of air).
Based on these results we can conclude that essential oils can be adequate solution for mntrol of B. cinerea, in vitro.
Кључне речи:
essential oils / Pinus silvestris / Petroselinum crispum / Origanum vulgare / Pimpinella anisum / Mentha piperita / Eucaliptus citriodora / Rosmarinus officinalis / Botrytis cinerea / controlИзвор:
International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November, 2013, 33-Издавач:
- ICNPU Bulgaria
Финансирање / пројекти:
Институција/група
Institut za povrtarstvoTY - CONF AU - Đorđević, Mladen AU - Damnjanović, Jelena AU - Šević, Milan AU - Kostić, Miroslav AU - Pavlović, Suzana AU - Marković, Tatjana AU - Zečević, Bogoljub PY - 2013 UR - http://RIVeC.institut-palanka.rs/handle/123456789/574 AB - Effectiveness of essential oils of Pinus silvestris, Petroselinum crispum, Origanum vulgare, Pimpinella anisum, Mentha piperita, Eucaliptus citriodora and Rosmarinus officinalis in control of Botrytis cinerea were tested, in vitro. Mycelial plug of B. cinerea was transferred to the center of the Petri plate. The oils were applied as a drop onto the inner side of the plate covers on the sterile filter paper, at the concetration of 0.04, 0.06, 0.1, 0.15, 0.3 and 0.6 µl/ml of air of the plate. To enable contact of volatile phase and pathogen, the plates were kept upside down and sealed with self-adhesive foil. Inhibitory effect of tested oils has been determined four days after setting the trial by calculating percentage of inhibition of radial growth of pathogen mycelium (PIRG), while minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) have been determined seven i.e. fourteen days after. For determining MFC, plates were ventilated in the sterile laminar flow for 30 minutes in order to remove volatiles of oils. Lowest MIC and MFC expressed O.vulgre applied at 0.04 µl/ml of air, followed by P. anisum applied at 0.06 µl/ml and 0.1µl/ml, M.piperita both MIC and MFC (0.15 µl/ml), E. citriodora (0.3 µI/ml), P. crispum (0.6 µl/ml) and P.silvestris (>0.6 µl/ml of air). Based on these results we can conclude that essential oils can be adequate solution for mntrol of B. cinerea, in vitro. PB - ICNPU Bulgaria C3 - International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November T1 - Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro SP - 33 UR - https://hdl.handle.net/21.15107/rcub_rivec_574 ER -
@conference{ author = "Đorđević, Mladen and Damnjanović, Jelena and Šević, Milan and Kostić, Miroslav and Pavlović, Suzana and Marković, Tatjana and Zečević, Bogoljub", year = "2013", abstract = "Effectiveness of essential oils of Pinus silvestris, Petroselinum crispum, Origanum vulgare, Pimpinella anisum, Mentha piperita, Eucaliptus citriodora and Rosmarinus officinalis in control of Botrytis cinerea were tested, in vitro. Mycelial plug of B. cinerea was transferred to the center of the Petri plate. The oils were applied as a drop onto the inner side of the plate covers on the sterile filter paper, at the concetration of 0.04, 0.06, 0.1, 0.15, 0.3 and 0.6 µl/ml of air of the plate. To enable contact of volatile phase and pathogen, the plates were kept upside down and sealed with self-adhesive foil. Inhibitory effect of tested oils has been determined four days after setting the trial by calculating percentage of inhibition of radial growth of pathogen mycelium (PIRG), while minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) have been determined seven i.e. fourteen days after. For determining MFC, plates were ventilated in the sterile laminar flow for 30 minutes in order to remove volatiles of oils. Lowest MIC and MFC expressed O.vulgre applied at 0.04 µl/ml of air, followed by P. anisum applied at 0.06 µl/ml and 0.1µl/ml, M.piperita both MIC and MFC (0.15 µl/ml), E. citriodora (0.3 µI/ml), P. crispum (0.6 µl/ml) and P.silvestris (>0.6 µl/ml of air). Based on these results we can conclude that essential oils can be adequate solution for mntrol of B. cinerea, in vitro.", publisher = "ICNPU Bulgaria", journal = "International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November", title = "Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro", pages = "33", url = "https://hdl.handle.net/21.15107/rcub_rivec_574" }
Đorđević, M., Damnjanović, J., Šević, M., Kostić, M., Pavlović, S., Marković, T.,& Zečević, B.. (2013). Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro. in International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November ICNPU Bulgaria., 33. https://hdl.handle.net/21.15107/rcub_rivec_574
Đorđević M, Damnjanović J, Šević M, Kostić M, Pavlović S, Marković T, Zečević B. Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro. in International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November. 2013;:33. https://hdl.handle.net/21.15107/rcub_rivec_574 .
Đorđević, Mladen, Damnjanović, Jelena, Šević, Milan, Kostić, Miroslav, Pavlović, Suzana, Marković, Tatjana, Zečević, Bogoljub, "Biological control of botrytis cinerea, pathogen of vegetables, using different essential oils in vitro" in International conference on natural products utilization : From plants to pharmacy shelf, ICNPU, Bansko, Bulgaria, 3-6 November (2013):33, https://hdl.handle.net/21.15107/rcub_rivec_574 .