Приказ основних података о документу
In vitro kultura kao deo procesa oplemenjivanja Brassica oleracea
In vitro culture as a part of Brassica oleracea breeding
dc.creator | Pavlović, Suzana | |
dc.creator | Adžić, Slađan | |
dc.creator | Cvikić, Dejan | |
dc.creator | Zdravković, Jasmina | |
dc.date.accessioned | 2022-12-09T11:43:13Z | |
dc.date.available | 2022-12-09T11:43:13Z | |
dc.date.issued | 2011 | |
dc.identifier.isbn | 978-86-87109-06-3 | |
dc.identifier.uri | http://RIVeC.institut-palanka.rs/handle/123456789/580 | |
dc.description.abstract | Kod četrnaest genotipova kupusa (Brassica oleracea var. capitata L.), koji su deo kolekcije Instituta za povrtarstvo, ispitana je sposobnost in vitro regeneracije pupoljaka. Ranih kupusa je bilo pet gonotipova, dok je kasnih kupusa bilo devet genotipova. Bočni pupoljci biljaka gajenih na otvorenom polju korišćeni su kao eksplantati. Kod svih genotipova bočni pupoljci su pokazali visok procenat regeneracije pupoljaka, od 80% do 100%. Inkubirani su na Murashige and Skoog’s (MS) hranljivoj podlozi sa dodatkom 1.0 i 2.0 mg l-i benziladenina (BA) ili 6-furfurilaminopurina (KIN) u kombinacija 0, 0.5 and 1.0 mg l-1 indole-3-butirične kiseline (IBA). Podloge koje su sadržale BA su bile optimalne, kako za regeneraciju pupoljaka, tako i za njhovu kasniju multiplikaciju. Kod obe grupe genotipova najveći indeks multiplikacije je postignut na podlozi sa dodatkom 2.0 mgl-1 BA i 1.0 mgl-1 IBA, kod R9 ranog genotipa (IM 8.53) i kod K1 kasnog genotipa (IM 10.06). Kod R1i ranog genotipa i kod K29 i K75 kasnih genotipova nije bilo multiplikacije na podlozi sa 1.0 mgl-1 KIN (IM 1.00). Takođe, najmanji indeks multiplikacije kod svih genotipova je primećen na podlogama sa KIN (bez ili u kombinaciji sa IBA). | sr |
dc.description.abstract | Fourteen genotypes of cabbage (Brassica oleracea var. capitata L.), that are a part of Institute for Vegetable Crops collection, were tested for their ability to re gen er ate shoots in vitro. Five of them are early, while nine are late genotypes. Lateral buds from plants grown in the open field were used as explants. In all genotypes lateral buds showed the high percentage of shoot formation ranged from 80% to 100%. They were in cu bated on Murashige and Skoog’s (MS) media supplemented with 1.0 and 2.0 mg l-1 of benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.5 and 1.0 mg l-1 indole-3-butyric acid (IBA). The BA-supplemented media were optimal for both growth and multiplication of shoots. In both groups of genotypes, the highest index of multiplication (IM) was achieved on m dium supplemented with 2.0 mgl-1 BA and 1.0 mgl-1 IBA, in R9 early genotype (IM 8.53) and K1 late ge no type (IM 10.06). R11 early and in K29 and K75 late ge no types had no multiplication on medium with 1.0 mgl-1 KIN (IM 1.00). Also, the lowest index of multiplication was observed in all genotypes on media supplemented with KIN (without or in combination with IBA). | sr |
dc.language.iso | sr | sr |
dc.language.iso | en | sr |
dc.publisher | Beograd : Društvo genetičara Srbije | sr |
dc.rights | restrictedAccess | sr |
dc.source | Zbornik apstrakata IV simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Okrugli sto: Kvalitet hrane - doprinos nauke = Book of abstracts of the IV Symposium of the Section of the breeding of organisms of the Serbian genetic society, Round table: Food quality - contribution of science, Kladovo, 2-6. oktobar | sr |
dc.subject | kupus | sr |
dc.subject | mikropropagacija | sr |
dc.subject | bočni pupoljci | sr |
dc.subject | indeks multiplikacije | sr |
dc.subject | cabbage | sr |
dc.subject | micropropagation | sr |
dc.subject | lateral buds | sr |
dc.subject | multiplication index | sr |
dc.title | In vitro kultura kao deo procesa oplemenjivanja Brassica oleracea | sr |
dc.title | In vitro culture as a part of Brassica oleracea breeding | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.citation.spage | 71 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_rivec_580 | |
dc.type.version | publishedVersion | sr |