Wheat colonization by determinate bacteria
Аутори
Mićanović, DanicaHirsch, Penny
Curnow, Phil
Zečević, Veselinka
Raičević, Vera
Knežević, Desimir
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain pseudomonads which contains the nptII gene, which codes for an aminoglycosidephosphotransferase, this enzyme confers antibiotic ressistance, to both neomycin and kanamycin. Number of bacteria was obtained from bulk soil, rhizosphere and rhizoplane, using phase contrast microscopy. Mutants were detected using selective agar for pseudomonas supplied with neomycin and genomic fingerprinting of bacteria used molecular genetic method ERIC-PCR. On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heteretroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 using ERIC-PCR and ...antibiotic- resistance phenotype on selective agar and indicates that it may be more competitive than the other two mutants.
Кључне речи:
colonization / marker genes / nitrogen fixation / wheatИзвор:
Savremena poljoprivreda, 2005, 54, 3-4, 312-317Издавач:
- Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet Novi Sad
- Novi Sad : Naučni Institut za ratarstvo i povrtarstvo
- Novi Sad : Dnevnik holding, „Dnevnik-Poljoprivrednik”
Напомена:
- Međunarodni naučni skup „Održiva poljoprivreda i proces evropske integracije” : International conference on sustainable agriculture and European integration processes September 19–24, 2004, Novi Sad, Serbia and Montenegro
Институција/група
Institut za povrtarstvoTY - JOUR AU - Mićanović, Danica AU - Hirsch, Penny AU - Curnow, Phil AU - Zečević, Veselinka AU - Raičević, Vera AU - Knežević, Desimir PY - 2005 UR - http://RIVeC.institut-palanka.rs/handle/123456789/875 AB - The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain pseudomonads which contains the nptII gene, which codes for an aminoglycosidephosphotransferase, this enzyme confers antibiotic ressistance, to both neomycin and kanamycin. Number of bacteria was obtained from bulk soil, rhizosphere and rhizoplane, using phase contrast microscopy. Mutants were detected using selective agar for pseudomonas supplied with neomycin and genomic fingerprinting of bacteria used molecular genetic method ERIC-PCR. On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heteretroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 using ERIC-PCR and antibiotic- resistance phenotype on selective agar and indicates that it may be more competitive than the other two mutants. PB - Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet Novi Sad PB - Novi Sad : Naučni Institut za ratarstvo i povrtarstvo PB - Novi Sad : Dnevnik holding, „Dnevnik-Poljoprivrednik” T2 - Savremena poljoprivreda T1 - Wheat colonization by determinate bacteria EP - 317 IS - 3-4 SP - 312 VL - 54 UR - https://hdl.handle.net/21.15107/rcub_rivec_875 ER -
@article{ author = "Mićanović, Danica and Hirsch, Penny and Curnow, Phil and Zečević, Veselinka and Raičević, Vera and Knežević, Desimir", year = "2005", abstract = "The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain pseudomonads which contains the nptII gene, which codes for an aminoglycosidephosphotransferase, this enzyme confers antibiotic ressistance, to both neomycin and kanamycin. Number of bacteria was obtained from bulk soil, rhizosphere and rhizoplane, using phase contrast microscopy. Mutants were detected using selective agar for pseudomonas supplied with neomycin and genomic fingerprinting of bacteria used molecular genetic method ERIC-PCR. On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heteretroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 using ERIC-PCR and antibiotic- resistance phenotype on selective agar and indicates that it may be more competitive than the other two mutants.", publisher = "Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet Novi Sad, Novi Sad : Naučni Institut za ratarstvo i povrtarstvo, Novi Sad : Dnevnik holding, „Dnevnik-Poljoprivrednik”", journal = "Savremena poljoprivreda", title = "Wheat colonization by determinate bacteria", pages = "317-312", number = "3-4", volume = "54", url = "https://hdl.handle.net/21.15107/rcub_rivec_875" }
Mićanović, D., Hirsch, P., Curnow, P., Zečević, V., Raičević, V.,& Knežević, D.. (2005). Wheat colonization by determinate bacteria. in Savremena poljoprivreda Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet Novi Sad., 54(3-4), 312-317. https://hdl.handle.net/21.15107/rcub_rivec_875
Mićanović D, Hirsch P, Curnow P, Zečević V, Raičević V, Knežević D. Wheat colonization by determinate bacteria. in Savremena poljoprivreda. 2005;54(3-4):312-317. https://hdl.handle.net/21.15107/rcub_rivec_875 .
Mićanović, Danica, Hirsch, Penny, Curnow, Phil, Zečević, Veselinka, Raičević, Vera, Knežević, Desimir, "Wheat colonization by determinate bacteria" in Savremena poljoprivreda, 54, no. 3-4 (2005):312-317, https://hdl.handle.net/21.15107/rcub_rivec_875 .