Freshwater algae Cladophora glomerata and Vaucheria sp. from Serbia as sources of bioactive compounds: chemical analysis and biological activities
Authors
Stefanović, Olgica D.![](/themes/MirageRIVeC/images/orcid.png)
Rakonjac, Aleksandra B.
![](/themes/MirageRIVeC/images/orcid.png)
Nikodijević, Danijela D.
![](/themes/MirageRIVeC/images/orcid.png)
Milojević, Sara D.
![](/themes/MirageRIVeC/images/orcid.png)
Dinić, Anica
Simić, Snežana B.
Article (Accepted Version)
Metadata
Show full item recordAbstract
We examined potential biological activities of two taxa of freshwater algae, Cladophora glomerata and Vaucheria sp., from Serbia. The total phenolic and pigment contents, antioxidant potential, antimicrobial, antibiofilm activities, and cytotoxicity of the ethanol and acetone extracts were evaluated. The extracts were also subjected to Fourier transform infrared spectroscopy (FTIR) analysis. The levels of total phenolic compounds, chlorophylls a and b, and carotenoids varied based on both the algal taxa and the type of extracts. FTIR analysis showed the presence of lipids, unsaturated fatty acids, protein, carbohydrates, and phenols in the algal extracts. The extracts had moderate DPPH radical scavenging activity and lower reducing power compared with ascorbic acid. The antimicrobial activity expressed as minimum inhibitory concentrations ranged from 0.31 mg/mL to 10 mg/mL. The strains of Staphylococcus aureus and Bacillus cereus isolated from food samples, as well as S. aureus ATCC 25...923, were the most sensitive. For the first time, the antibiofilm activity test revealed 98.7% inhibition of S. aureus biofilm formation. The extracts exhibited cytotoxic effects on choriocarcinoma JAR cells but without selectivity on normal fetal lung fibroblast MRC-5 cells. This is the first report on the biological activities of freshwater macroalgae from Serbia.
Keywords:
freshwater algae / algal extracts / antioxidant activity / antimicrobial activity / antibiofilmactivity / cytotoxicitySource:
Archives of Biological Sciences, , 00, 12-12Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200122 (University of Kragujevac, Faculty of Science) (RS-MESTD-inst-2020-200122)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200216 (Institute for Vegetable Crops, Smederevska Palanka) (RS-MESTD-inst-2020-200216)
URI
https://doiserbia.nb.rs/Article.aspx?ID=0354-46642400012Shttp://RIVeC.institut-palanka.rs/handle/123456789/895
Collections
Institution/Community
Institut za povrtarstvoTY - JOUR AU - Stefanović, Olgica D. AU - Rakonjac, Aleksandra B. AU - Nikodijević, Danijela D. AU - Milojević, Sara D. AU - Dinić, Anica AU - Simić, Snežana B. PY - online first UR - https://doiserbia.nb.rs/Article.aspx?ID=0354-46642400012S UR - http://RIVeC.institut-palanka.rs/handle/123456789/895 AB - We examined potential biological activities of two taxa of freshwater algae, Cladophora glomerata and Vaucheria sp., from Serbia. The total phenolic and pigment contents, antioxidant potential, antimicrobial, antibiofilm activities, and cytotoxicity of the ethanol and acetone extracts were evaluated. The extracts were also subjected to Fourier transform infrared spectroscopy (FTIR) analysis. The levels of total phenolic compounds, chlorophylls a and b, and carotenoids varied based on both the algal taxa and the type of extracts. FTIR analysis showed the presence of lipids, unsaturated fatty acids, protein, carbohydrates, and phenols in the algal extracts. The extracts had moderate DPPH radical scavenging activity and lower reducing power compared with ascorbic acid. The antimicrobial activity expressed as minimum inhibitory concentrations ranged from 0.31 mg/mL to 10 mg/mL. The strains of Staphylococcus aureus and Bacillus cereus isolated from food samples, as well as S. aureus ATCC 25923, were the most sensitive. For the first time, the antibiofilm activity test revealed 98.7% inhibition of S. aureus biofilm formation. The extracts exhibited cytotoxic effects on choriocarcinoma JAR cells but without selectivity on normal fetal lung fibroblast MRC-5 cells. This is the first report on the biological activities of freshwater macroalgae from Serbia. T2 - Archives of Biological Sciences T1 - Freshwater algae Cladophora glomerata and Vaucheria sp. from Serbia as sources of bioactive compounds: chemical analysis and biological activities EP - 12 IS - 00 SP - 12 UR - https://hdl.handle.net/21.15107/rcub_rivec_895 ER -
@article{ author = "Stefanović, Olgica D. and Rakonjac, Aleksandra B. and Nikodijević, Danijela D. and Milojević, Sara D. and Dinić, Anica and Simić, Snežana B.", year = "online first", abstract = "We examined potential biological activities of two taxa of freshwater algae, Cladophora glomerata and Vaucheria sp., from Serbia. The total phenolic and pigment contents, antioxidant potential, antimicrobial, antibiofilm activities, and cytotoxicity of the ethanol and acetone extracts were evaluated. The extracts were also subjected to Fourier transform infrared spectroscopy (FTIR) analysis. The levels of total phenolic compounds, chlorophylls a and b, and carotenoids varied based on both the algal taxa and the type of extracts. FTIR analysis showed the presence of lipids, unsaturated fatty acids, protein, carbohydrates, and phenols in the algal extracts. The extracts had moderate DPPH radical scavenging activity and lower reducing power compared with ascorbic acid. The antimicrobial activity expressed as minimum inhibitory concentrations ranged from 0.31 mg/mL to 10 mg/mL. The strains of Staphylococcus aureus and Bacillus cereus isolated from food samples, as well as S. aureus ATCC 25923, were the most sensitive. For the first time, the antibiofilm activity test revealed 98.7% inhibition of S. aureus biofilm formation. The extracts exhibited cytotoxic effects on choriocarcinoma JAR cells but without selectivity on normal fetal lung fibroblast MRC-5 cells. This is the first report on the biological activities of freshwater macroalgae from Serbia.", journal = "Archives of Biological Sciences", title = "Freshwater algae Cladophora glomerata and Vaucheria sp. from Serbia as sources of bioactive compounds: chemical analysis and biological activities", pages = "12-12", number = "00", url = "https://hdl.handle.net/21.15107/rcub_rivec_895" }
Stefanović, O. D., Rakonjac, A. B., Nikodijević, D. D., Milojević, S. D., Dinić, A.,& Simić, S. B.. (online first). Freshwater algae Cladophora glomerata and Vaucheria sp. from Serbia as sources of bioactive compounds: chemical analysis and biological activities. in Archives of Biological Sciences(00), 12-12. https://hdl.handle.net/21.15107/rcub_rivec_895
Stefanović OD, Rakonjac AB, Nikodijević DD, Milojević SD, Dinić A, Simić SB. Freshwater algae Cladophora glomerata and Vaucheria sp. from Serbia as sources of bioactive compounds: chemical analysis and biological activities. in Archives of Biological Sciences. onli;(00):12-12. https://hdl.handle.net/21.15107/rcub_rivec_895 .
Stefanović, Olgica D., Rakonjac, Aleksandra B., Nikodijević, Danijela D., Milojević, Sara D., Dinić, Anica, Simić, Snežana B., "Freshwater algae Cladophora glomerata and Vaucheria sp. from Serbia as sources of bioactive compounds: chemical analysis and biological activities" in Archives of Biological Sciences, no. 00 (online first):12-12, https://hdl.handle.net/21.15107/rcub_rivec_895 .