Citogenetički efekat etilmetansulfonata u paprike
Cytologic Effect of Ethyl Methanesulfonate in Pepper
Чланак у часопису (Објављена верзија)
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Osušeno seme paprike je tretirano 1%, 2% i 3% rastvorom etil metan sulfonatom (EMS) u trajanju od 6, 8 i 12 sati, na temperaturi od 20°C i pH podešenom na 7.
Podaci o klijavosti i preživljavanju ukazali su na prilično smanjenje vitalnosti sa povećanjem koncentracije EMS-a i produženim tretmanom. Preživljavanje se smanjivalo za 20% sa produžavanjem 1% EMS tretmana sa 6 sati na 12 sati, a za 34% sa povećanjem koncentracije EMS sa 1% na 3% (u 8-časovnom tretmanu).
Citološke analize preživelih M1 biljaka dobijenih iz semena tretiranih sa 1% EMS, pokazale su da je oko 9% ćelija u metafazi I sadržalo jedan neorijentisani bivalent, što ukazuje na blagu abnormalnost centromera. Ovo je bila jedina hromozomska aberacija primećena posle 1% EMS tretmana. Posle 2% EMS tretmana neorijentacija bivalenta je porasla na 13,3% i 6,6% kod ćelija u anafazi i koje su sadržale hromatinske mostove.
Učestalost hromozomskih aberacija su značajno povećane 3% EMS tretmanom. Najčešći tipovi hromozomskih... aberacija su bile pseudo-izohromozomi (25%), univalentni u metafazi I (8%), dicentrični hromatinski mostovi u anafazi I (9,26%), zaostali i mikronukleusi u telofazi I i II.
Rezultati našeg istraživanja takođe potvrđuju da je centromerni region najpodložniji efektima EMS. Povreda centromera kod paprike izazvana je 1% EMS, dok su ozbiljnije strukturne hromozomske aberacije indukovane tek 2% i 3% EMS. Takođe, na osnovu ovih činjenica smo zaključili da su hromozomi paprike manje podložni mutagenom dejstvu EMS od hromozoma nekih drugih biljnih vrsta.
Air dry seed of pepper was treated with 1%, 2% and 3% ethyl methane sulfonate (EMS) for duration of 6, 8 and 12 hours, at a temperature of 20°C and pH adjusted to 7.
Data on the germinability and survival show a rather regular decrease of vitality with increased concentration of EMS and prolonged treatment. The survival is reduced by 20% when treatment with 1%, EMS is extended from 6 hours to 12 hours, and 34% by the increase of concentration of EMS from 1% to 31% (in the 8-hour treatment).
Cytologic analyses of survived M1-plants produced from seeds treated with 1% EMS, revealed that about 9% of metaphase I cells contained one non-oriented bivalent, an indication of a slight centromere abnormality. This was the only chromosome aberration observed after 1% EMS treatment- After 2% EMS treatment non-orientation of bivalents increased to 13.3% and 6.6% anaphase, I cells contained chromatin bridges.
The frequency of chromosome aberrations was increased greatly by the 3% EMS tre...atment. The most frequent kinds of chromosome aberrations we-re pseudo-isochromosomes (25%), univalent in metaphase I (8%), dicentric chromatin bridges in anaphase I (9,26%), laggards and micronuclei in telophase I and II.
The results of our research confirm also that the centromere region is the most susceptible to the effects of EMS. Centromere injury in pepper was induced by 1% EMS, whereas more serious structural chromosome aberrations were induced only after 2% and 3% EMS. Also, on the basis of these facts we have concluded that the chromosomes of pepper are less susceptible than the chromosomes of some other plant species to the mutagenic effect of EMS.
Кључне речи:
ethyl methane sulfonate (EMS) / pepper seedИзвор:
Arhiv za poljoprivredne nauke, 1971, XXIV, 85, 53-58Издавач:
- Beograd : Ministarstvo poljoprivrede i šumarstva
- Beograd : Savez poljoprivrednih inženjera i tehničara Jugoslavije
Финансирање / пројекти:
- Ministarstvo poljoprivrede SAD PL 480, Projekat E30-CR-27
Институција/група
Institut za povrtarstvoTY - JOUR AU - Spasojević, Vladimir AU - Webb, R.E. PY - 1971 UR - http://RIVeC.institut-palanka.rs/handle/123456789/673 AB - Osušeno seme paprike je tretirano 1%, 2% i 3% rastvorom etil metan sulfonatom (EMS) u trajanju od 6, 8 i 12 sati, na temperaturi od 20°C i pH podešenom na 7. Podaci o klijavosti i preživljavanju ukazali su na prilično smanjenje vitalnosti sa povećanjem koncentracije EMS-a i produženim tretmanom. Preživljavanje se smanjivalo za 20% sa produžavanjem 1% EMS tretmana sa 6 sati na 12 sati, a za 34% sa povećanjem koncentracije EMS sa 1% na 3% (u 8-časovnom tretmanu). Citološke analize preživelih M1 biljaka dobijenih iz semena tretiranih sa 1% EMS, pokazale su da je oko 9% ćelija u metafazi I sadržalo jedan neorijentisani bivalent, što ukazuje na blagu abnormalnost centromera. Ovo je bila jedina hromozomska aberacija primećena posle 1% EMS tretmana. Posle 2% EMS tretmana neorijentacija bivalenta je porasla na 13,3% i 6,6% kod ćelija u anafazi i koje su sadržale hromatinske mostove. Učestalost hromozomskih aberacija su značajno povećane 3% EMS tretmanom. Najčešći tipovi hromozomskih aberacija su bile pseudo-izohromozomi (25%), univalentni u metafazi I (8%), dicentrični hromatinski mostovi u anafazi I (9,26%), zaostali i mikronukleusi u telofazi I i II. Rezultati našeg istraživanja takođe potvrđuju da je centromerni region najpodložniji efektima EMS. Povreda centromera kod paprike izazvana je 1% EMS, dok su ozbiljnije strukturne hromozomske aberacije indukovane tek 2% i 3% EMS. Takođe, na osnovu ovih činjenica smo zaključili da su hromozomi paprike manje podložni mutagenom dejstvu EMS od hromozoma nekih drugih biljnih vrsta. AB - Air dry seed of pepper was treated with 1%, 2% and 3% ethyl methane sulfonate (EMS) for duration of 6, 8 and 12 hours, at a temperature of 20°C and pH adjusted to 7. Data on the germinability and survival show a rather regular decrease of vitality with increased concentration of EMS and prolonged treatment. The survival is reduced by 20% when treatment with 1%, EMS is extended from 6 hours to 12 hours, and 34% by the increase of concentration of EMS from 1% to 31% (in the 8-hour treatment). Cytologic analyses of survived M1-plants produced from seeds treated with 1% EMS, revealed that about 9% of metaphase I cells contained one non-oriented bivalent, an indication of a slight centromere abnormality. This was the only chromosome aberration observed after 1% EMS treatment- After 2% EMS treatment non-orientation of bivalents increased to 13.3% and 6.6% anaphase, I cells contained chromatin bridges. The frequency of chromosome aberrations was increased greatly by the 3% EMS treatment. The most frequent kinds of chromosome aberrations we-re pseudo-isochromosomes (25%), univalent in metaphase I (8%), dicentric chromatin bridges in anaphase I (9,26%), laggards and micronuclei in telophase I and II. The results of our research confirm also that the centromere region is the most susceptible to the effects of EMS. Centromere injury in pepper was induced by 1% EMS, whereas more serious structural chromosome aberrations were induced only after 2% and 3% EMS. Also, on the basis of these facts we have concluded that the chromosomes of pepper are less susceptible than the chromosomes of some other plant species to the mutagenic effect of EMS. PB - Beograd : Ministarstvo poljoprivrede i šumarstva PB - Beograd : Savez poljoprivrednih inženjera i tehničara Jugoslavije T2 - Arhiv za poljoprivredne nauke T1 - Citogenetički efekat etilmetansulfonata u paprike T1 - Cytologic Effect of Ethyl Methanesulfonate in Pepper EP - 58 IS - 85 SP - 53 VL - XXIV UR - https://hdl.handle.net/21.15107/rcub_rivec_673 ER -
@article{ author = "Spasojević, Vladimir and Webb, R.E.", year = "1971", abstract = "Osušeno seme paprike je tretirano 1%, 2% i 3% rastvorom etil metan sulfonatom (EMS) u trajanju od 6, 8 i 12 sati, na temperaturi od 20°C i pH podešenom na 7. Podaci o klijavosti i preživljavanju ukazali su na prilično smanjenje vitalnosti sa povećanjem koncentracije EMS-a i produženim tretmanom. Preživljavanje se smanjivalo za 20% sa produžavanjem 1% EMS tretmana sa 6 sati na 12 sati, a za 34% sa povećanjem koncentracije EMS sa 1% na 3% (u 8-časovnom tretmanu). Citološke analize preživelih M1 biljaka dobijenih iz semena tretiranih sa 1% EMS, pokazale su da je oko 9% ćelija u metafazi I sadržalo jedan neorijentisani bivalent, što ukazuje na blagu abnormalnost centromera. Ovo je bila jedina hromozomska aberacija primećena posle 1% EMS tretmana. Posle 2% EMS tretmana neorijentacija bivalenta je porasla na 13,3% i 6,6% kod ćelija u anafazi i koje su sadržale hromatinske mostove. Učestalost hromozomskih aberacija su značajno povećane 3% EMS tretmanom. Najčešći tipovi hromozomskih aberacija su bile pseudo-izohromozomi (25%), univalentni u metafazi I (8%), dicentrični hromatinski mostovi u anafazi I (9,26%), zaostali i mikronukleusi u telofazi I i II. Rezultati našeg istraživanja takođe potvrđuju da je centromerni region najpodložniji efektima EMS. Povreda centromera kod paprike izazvana je 1% EMS, dok su ozbiljnije strukturne hromozomske aberacije indukovane tek 2% i 3% EMS. Takođe, na osnovu ovih činjenica smo zaključili da su hromozomi paprike manje podložni mutagenom dejstvu EMS od hromozoma nekih drugih biljnih vrsta., Air dry seed of pepper was treated with 1%, 2% and 3% ethyl methane sulfonate (EMS) for duration of 6, 8 and 12 hours, at a temperature of 20°C and pH adjusted to 7. Data on the germinability and survival show a rather regular decrease of vitality with increased concentration of EMS and prolonged treatment. The survival is reduced by 20% when treatment with 1%, EMS is extended from 6 hours to 12 hours, and 34% by the increase of concentration of EMS from 1% to 31% (in the 8-hour treatment). Cytologic analyses of survived M1-plants produced from seeds treated with 1% EMS, revealed that about 9% of metaphase I cells contained one non-oriented bivalent, an indication of a slight centromere abnormality. This was the only chromosome aberration observed after 1% EMS treatment- After 2% EMS treatment non-orientation of bivalents increased to 13.3% and 6.6% anaphase, I cells contained chromatin bridges. The frequency of chromosome aberrations was increased greatly by the 3% EMS treatment. The most frequent kinds of chromosome aberrations we-re pseudo-isochromosomes (25%), univalent in metaphase I (8%), dicentric chromatin bridges in anaphase I (9,26%), laggards and micronuclei in telophase I and II. The results of our research confirm also that the centromere region is the most susceptible to the effects of EMS. Centromere injury in pepper was induced by 1% EMS, whereas more serious structural chromosome aberrations were induced only after 2% and 3% EMS. Also, on the basis of these facts we have concluded that the chromosomes of pepper are less susceptible than the chromosomes of some other plant species to the mutagenic effect of EMS.", publisher = "Beograd : Ministarstvo poljoprivrede i šumarstva, Beograd : Savez poljoprivrednih inženjera i tehničara Jugoslavije", journal = "Arhiv za poljoprivredne nauke", title = "Citogenetički efekat etilmetansulfonata u paprike, Cytologic Effect of Ethyl Methanesulfonate in Pepper", pages = "58-53", number = "85", volume = "XXIV", url = "https://hdl.handle.net/21.15107/rcub_rivec_673" }
Spasojević, V.,& Webb, R.E.. (1971). Citogenetički efekat etilmetansulfonata u paprike. in Arhiv za poljoprivredne nauke Beograd : Ministarstvo poljoprivrede i šumarstva., XXIV(85), 53-58. https://hdl.handle.net/21.15107/rcub_rivec_673
Spasojević V, Webb R. Citogenetički efekat etilmetansulfonata u paprike. in Arhiv za poljoprivredne nauke. 1971;XXIV(85):53-58. https://hdl.handle.net/21.15107/rcub_rivec_673 .
Spasojević, Vladimir, Webb, R.E., "Citogenetički efekat etilmetansulfonata u paprike" in Arhiv za poljoprivredne nauke, XXIV, no. 85 (1971):53-58, https://hdl.handle.net/21.15107/rcub_rivec_673 .