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Citogenetički efekat etilmetansulfonata u paprike
Cytologic Effect of Ethyl Methanesulfonate in Pepper
dc.creator | Spasojević, Vladimir | |
dc.creator | Webb, R.E. | |
dc.date.accessioned | 2023-06-22T12:37:32Z | |
dc.date.available | 2023-06-22T12:37:32Z | |
dc.date.issued | 1971 | |
dc.identifier.issn | 0365-5601 | |
dc.identifier.uri | http://RIVeC.institut-palanka.rs/handle/123456789/673 | |
dc.description.abstract | Osušeno seme paprike je tretirano 1%, 2% i 3% rastvorom etil metan sulfonatom (EMS) u trajanju od 6, 8 i 12 sati, na temperaturi od 20°C i pH podešenom na 7. Podaci o klijavosti i preživljavanju ukazali su na prilično smanjenje vitalnosti sa povećanjem koncentracije EMS-a i produženim tretmanom. Preživljavanje se smanjivalo za 20% sa produžavanjem 1% EMS tretmana sa 6 sati na 12 sati, a za 34% sa povećanjem koncentracije EMS sa 1% na 3% (u 8-časovnom tretmanu). Citološke analize preživelih M1 biljaka dobijenih iz semena tretiranih sa 1% EMS, pokazale su da je oko 9% ćelija u metafazi I sadržalo jedan neorijentisani bivalent, što ukazuje na blagu abnormalnost centromera. Ovo je bila jedina hromozomska aberacija primećena posle 1% EMS tretmana. Posle 2% EMS tretmana neorijentacija bivalenta je porasla na 13,3% i 6,6% kod ćelija u anafazi i koje su sadržale hromatinske mostove. Učestalost hromozomskih aberacija su značajno povećane 3% EMS tretmanom. Najčešći tipovi hromozomskih aberacija su bile pseudo-izohromozomi (25%), univalentni u metafazi I (8%), dicentrični hromatinski mostovi u anafazi I (9,26%), zaostali i mikronukleusi u telofazi I i II. Rezultati našeg istraživanja takođe potvrđuju da je centromerni region najpodložniji efektima EMS. Povreda centromera kod paprike izazvana je 1% EMS, dok su ozbiljnije strukturne hromozomske aberacije indukovane tek 2% i 3% EMS. Takođe, na osnovu ovih činjenica smo zaključili da su hromozomi paprike manje podložni mutagenom dejstvu EMS od hromozoma nekih drugih biljnih vrsta. | sr |
dc.description.abstract | Air dry seed of pepper was treated with 1%, 2% and 3% ethyl methane sulfonate (EMS) for duration of 6, 8 and 12 hours, at a temperature of 20°C and pH adjusted to 7. Data on the germinability and survival show a rather regular decrease of vitality with increased concentration of EMS and prolonged treatment. The survival is reduced by 20% when treatment with 1%, EMS is extended from 6 hours to 12 hours, and 34% by the increase of concentration of EMS from 1% to 31% (in the 8-hour treatment). Cytologic analyses of survived M1-plants produced from seeds treated with 1% EMS, revealed that about 9% of metaphase I cells contained one non-oriented bivalent, an indication of a slight centromere abnormality. This was the only chromosome aberration observed after 1% EMS treatment- After 2% EMS treatment non-orientation of bivalents increased to 13.3% and 6.6% anaphase, I cells contained chromatin bridges. The frequency of chromosome aberrations was increased greatly by the 3% EMS treatment. The most frequent kinds of chromosome aberrations we-re pseudo-isochromosomes (25%), univalent in metaphase I (8%), dicentric chromatin bridges in anaphase I (9,26%), laggards and micronuclei in telophase I and II. The results of our research confirm also that the centromere region is the most susceptible to the effects of EMS. Centromere injury in pepper was induced by 1% EMS, whereas more serious structural chromosome aberrations were induced only after 2% and 3% EMS. Also, on the basis of these facts we have concluded that the chromosomes of pepper are less susceptible than the chromosomes of some other plant species to the mutagenic effect of EMS. | en |
dc.language.iso | sr | sr |
dc.publisher | Beograd : Ministarstvo poljoprivrede i šumarstva | sr |
dc.publisher | Beograd : Savez poljoprivrednih inženjera i tehničara Jugoslavije | sr |
dc.relation | Ministarstvo poljoprivrede SAD PL 480, Projekat E30-CR-27 | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Arhiv za poljoprivredne nauke | sr |
dc.subject | ethyl methane sulfonate (EMS) | sr |
dc.subject | pepper seed | sr |
dc.title | Citogenetički efekat etilmetansulfonata u paprike | sr |
dc.title | Cytologic Effect of Ethyl Methanesulfonate in Pepper | en |
dc.type | article | sr |
dc.rights.license | BY | sr |
dc.citation.epage | 58 | |
dc.citation.issue | 85 | |
dc.citation.spage | 53 | |
dc.citation.volume | XXIV | |
dc.identifier.fulltext | http://RIVeC.institut-palanka.rs/bitstream/id/2186/S88.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_rivec_673 | |
dc.type.version | publishedVersion | sr |