Investigation of sublines of Kragujevačka 56 variety of wheat (Tr. aestivum ssp. vulgare)

Abstract

Chosen sublines of the Kg 56 cultivar were examined with main goals of detecting polymorphism within it and of identifying superior genotypes for some traits. Some morphological, physiological and technological traits were investigated, as well as resistance to disease (in general, traits with high heritability) using standard methods. Polymorphism of sublines was detected within the Kg. 56 cultivar and confirmed on a biochemical basis, by polyacrylamide gel electrophoresis of grain endosperm gliadins. Using the acid PAGE—method 4 different genotypes were identified in relation to gliadin composition, and on the basis of the remaining analyses 11 sublines were identified.

U cilju da se ispita polimorfizam unutar sorte Kg 56 i da se identifikuju superiorni genotipovi za pojedina svojstva proučavana su odabrane sublinije ove sorte. Ispitivanjima su obuhvaćene neke morfološke, fiziološke i tehnološke osobine, kao i otpornost na bolesti (uglavnom osobine visokih heritabilnosti) korišćenjem standardnih metoda. Na osnovu ovih istraživanja ustanovljen je polimorfizam sublinija unutar sorte Kg56, a što je potvrđeno i na biohemijskom nivou, analizom glijadina pomoću poliakrilamidne gel elektroforeze. Metodom 'acid PAGE' Lookhart (1982) identifikovana su 4 različita genotipa u odnosu na sastav glijadina, a na osnovu ostalih analiza identifikovano je 11 sublinija.