Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
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2014
Authors
Gašić, KatarinaIvanović, Milan
Kuzmanović, Nemanja
Prokić, Anđelka
Obradović, Aleksa
Šević, Milan
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In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
Keywords:
bacteriophages / host range / fire blight / biological controlSource:
Acta Horticulturae, 2014, 1056, 123-126Publisher:
- International Society for Horticultural Science
Funding / projects:
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
DOI: 10.17660/ActaHortic.2014.1056.17
ISBN: 978-94-62610-46-0
ISSN: 0567-7572
WoS: 000357733400017
Scopus: 2-s2.0-84912099519
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Institut za povrtarstvoTY - CONF AU - Gašić, Katarina AU - Ivanović, Milan AU - Kuzmanović, Nemanja AU - Prokić, Anđelka AU - Obradović, Aleksa AU - Šević, Milan PY - 2014 UR - http://RIVeC.institut-palanka.rs/handle/123456789/154 AB - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation. PB - International Society for Horticultural Science C3 - Acta Horticulturae T1 - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential EP - 126 SP - 123 VL - 1056 DO - 10.17660/ActaHortic.2014.1056.17 ER -
@conference{ author = "Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Anđelka and Obradović, Aleksa and Šević, Milan", year = "2014", abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.", publisher = "International Society for Horticultural Science", journal = "Acta Horticulturae", title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential", pages = "126-123", volume = "1056", doi = "10.17660/ActaHortic.2014.1056.17" }
Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae International Society for Horticultural Science., 1056, 123-126. https://doi.org/10.17660/ActaHortic.2014.1056.17
Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae. 2014;1056:123-126. doi:10.17660/ActaHortic.2014.1056.17 .
Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Anđelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in Acta Horticulturae, 1056 (2014):123-126, https://doi.org/10.17660/ActaHortic.2014.1056.17 . .